油莎豆快速繁殖体系的研究
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摘要
为建立油莎豆(Cyperus esculentus)的快繁体系,以茎尖为外植体,MS为基本培养基,采用正交设计法研究了6-BA、KT和NAA组合对丛生芽增殖和生根的影响。结果表明,3种生长调节剂对油莎豆丛生芽增殖的影响为6-BA> NAA> KT,最佳生长调节剂组合为1.0 mg L~(-1) 6-BA+0.2 mg L~(-1) KT,培养4周的增殖系数为7.58。MS培养基为最优生根培养基,生根率可达88.10%,平均生根数为2.04条。这为高效繁育油莎豆优质种苗和种质资源优化奠定基础。
In order to construct rapid propagation system of yellow nutsedge(Cyperus esculentus), the effect of growth regulator combinations, including 6-BA, KT and NAA, was studied by using orthogonal design method with shoot tip as explants on basic medium of MS. The results showed that the effects of growth regulators on proliferation of multiole shoots was in order of 6-BA >NAA> KT. The optimal growth regulator combination was 1.0 mg L~(-1) 6-BA+0.2 mg L~(-1) KT with proliferation coefficient of 7.58 after cultured for 4 weeks. MS medium was optimal rooting medium with rooting rate of 88.10%, and an average roots was 2.04. So, these would lay a foundation for efficient breeding of high quality seedlings and optimization of germplasm resource of yellow nutsedge.
In order to construct rapid propagation system of yellow nutsedge(Cyperus esculentus), the effect of growth regulator combinations, including 6-BA, KT and NAA, was studied by using orthogonal design method with shoot tip as explants on basic medium of MS. The results showed that the effects of growth regulators on proliferation of multiole shoots was in order of 6-BA >NAA> KT. The optimal growth regulator combination was 1.0 mg L~(-1) 6-BA+0.2 mg L~(-1) KT with proliferation coefficient of 7.58 after cultured for 4 weeks. MS medium was optimal rooting medium with rooting rate of 88.10%, and an average roots was 2.04. So, these would lay a foundation for efficient breeding of high quality seedlings and optimization of germplasm resource of yellow nutsedge.
引文
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[17]FENG F,YE C H,GUO J Y,et al.Study on the effects of growth inhibitors on the growth of Dioscorea alata Linn.in vitro[J].Crops,2007,23(2):29-31.丰锋,叶春海,郭锦云,等.植物生长抑制剂对淮山药组培苗生长的影响[J].作物杂志,2007,23(2):29-31.
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[20]WU Q.The regeneration system of yellow nutsedge and the polyploid induction[D].Lanzhou:Lanzhou University,2009:1-42.吴琼.油莎豆再生体系的建立及多倍体诱导[D].兰州:兰州大学,2009:1-42.
[2]DUAN J X,MA J.Cultivation of Yellow Nutsedge[M].Beijing:Science Popular Press,1983:1-44.段俊喜,马杰.油莎豆栽培[M].北京:科学普及出版社,1983:1-44.
[3]YANG Z L.Characteristics and research progress of Cyperus esculent[J].N Hort,2017,41(17):192-201.doi:10.11937/bfyy.20170541.阳振乐.油莎豆的特性及其研究进展[J].北方园艺,2017,41(17):192-201.doi:10.11937/bfyy.2017 0541.
[4]China Oil Plant Compilation Group.China Oil Plant[M].Beijing:Science Press,1987:33-35.中国油脂植物编写组.中国油脂植物[M].北京:科学出版社,1987:33-35.
[5]WU Y X.The oil of tigernet(Cyperus esculentus L.)[J].Grain Sci Technol Econ,1993(1):30.吴艳霞.油莎豆油[J].粮油科技与经济,1993(1):30.
[6]TILKAT E,ONAY A.Direct shoot organogenesis from in vitroderived mature leaf explants of pistachio[J].In Vitro Cell Dev Biol Plant,2009,45(1):92-98.doi:10.1007/s11627-008-9168-4.
[7]QU P M,LONG C L,CHENG Z Y,et al.Tissue culture and rapid propagation of Cyperus esculentus L.var.sativus Boeck[J].Plant Physiol Commun,2007,43(2):331.瞿萍梅,龙春林,程治英,等.油莎豆的组织培养和快速繁殖[J].植物生理学通讯,2007,43(2):331.
[8]ZUO J J,YAN G Y,AN X N,et al.Tissue culture of Atractylodes chinensis by orthogonal design[J].Chin Agric Bull,2017,33(11):21-24.doi:10.11924/j.issn.1000-6850.casb16070023.左静静,闫贵云,安晓宁,等.正交设计优化北苍术组织培养研究[J].中国农学通报,2017,33(11):21-24.doi:10.11924/j.issn.1000-6850.casb16070023.
[9]WANG Y Y,LIN X H,HONG X.Studies on explants selection and callus induction of Anoectochilus roxburghii[J].Subtrop Plant Sci,2011,40(3):41-43.doi:10.3969/j.issn.1009-7791.2011.03.011.王雅英,林小华,洪璇.金线莲外植体筛选及愈伤组织诱导研究[J].亚热带植物科学,2011,40(3):41-43.doi:10.3969/j.issn.1009-7791.2011.03.011.
[10]YAN H B,YANG L T,LI Y R.Axillary shoot proliferation and tuberization of Dioscorea fordii Prain et Burk[J].Plant Cell Tiss Organ Cult,2011,104(2):193-198.doi:10.1007/s11240-010-9818-1.
[11]CHEN Y L,PENG J P,LIU F,et al.An orthogonal design study of proliferation and flowering in vitro of Dianthus caryophyllus L.[J].Chin J Trop Crops,2017,38(10):1907-1912.doi:10.3969/j.issn.1000-2561.2017.10.022.陈雨露,彭洁平,刘芳,等.基于正交设计的康乃馨试管苗增殖和开花诱导研究[J].热带作物学报,2017,38(10):1907-1912.doi:10.3969/j.issn.1000-2561.2017.10.022.
[12]GAO J M,LI J Z,LUO C B,et al.Callus induction and plant regeneration in Alternanthera philoxeroides[J].Mol Biol Rep,2011,38(2):1413-1417.doi:10.1007/s11033-010-0245-5.
[13]JIN S,CHEN Z B,LI Y C,et al.Establishment of rapid propagation system of Pinellia ternata[J].Lishizhen Med Mat Med Res,2017,28(7):1759-1761.靳松,陈泽斌,李育川,等.半夏组培快繁体系的建立[J].时珍国医国药,2017,28(7):1759-1761.
[14]CHEN Z H,HUA S M,LI L H,et al.Optimization of technical conditions for tissue culture and rapid propagation of Chinese Yam[J].Fujian J Agric Sci,2018,33(2):164-170.陈芝华,华树妹,李丽红,等.山药组培快繁技术条件的优化[J].福建农业学报,2018,33(2):164-170.
[15]ZHANG H Y,WEI S L.Rapid propagation of Eleocharis tuberosa plantlet in vitro[J].J Guangxi Agric Biol Sci,2007,26(S1):99-102.张慧英,韦绍丽.荸荠试管苗的快速繁殖[J].广西农业生物科学,2007,26(S1):99-102.
[16]HAN X Y,YAN R X,YIN J M,et al.Research on tissue culture for rapid propagation and in vitro bulbils regeneration system of Dioscorea opposita Thunb.[J].Acta Bot Boreali-Occid Sin,2013,33(10):2120-2125.韩晓勇,闫瑞霞,殷剑美,等.铁棍山药组织培养快繁及试管珠芽离体再生体系研究[J].西北植物学报,2013,33(10):2120-2125.
[17]FENG F,YE C H,GUO J Y,et al.Study on the effects of growth inhibitors on the growth of Dioscorea alata Linn.in vitro[J].Crops,2007,23(2):29-31.丰锋,叶春海,郭锦云,等.植物生长抑制剂对淮山药组培苗生长的影响[J].作物杂志,2007,23(2):29-31.
[18]CAI J R.Study on tissue culture of Dioscorea opposita[D].Fuzhou:Fujian Agriculture and Forestry University,2010:1-32.蔡建荣.山药组织培养技术的研究[D].福州:福建农林大学,2010:1-32.
[19]PAN M,HUANG S,WANG J F,et al.Study on in vitro cultivation conditions and cultivating techniques of Dioscorea opposita[J].Guizhou Agric Sci,2014,42(3):125-129.潘梅,黄赛,王景飞,等.山药茎段的离体培养与育苗基质筛选[J].贵州农业科学,2014,42(3):125-129.
[20]WU Q.The regeneration system of yellow nutsedge and the polyploid induction[D].Lanzhou:Lanzhou University,2009:1-42.吴琼.油莎豆再生体系的建立及多倍体诱导[D].兰州:兰州大学,2009:1-42.