远志对DPN大鼠背根节神经元损伤的预防保护作用
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摘要
目的:观察远志对糖尿病周围神经病变(DPN)大鼠坐骨神经相关神经元胞体损伤的预防保护作用。方法:Wistar大鼠随机分为3组(n=12):空白对照组,DPN模型组及远志预防组。DPN模型组和远志预防组大鼠建立链脲佐菌素致糖尿病模型后,远志预防组大鼠给予远志(2.7g/kg/d)灌胃6周。以大鼠尾部感觉神经传导速度(SNCV)<30m/s为标准建立DPN大鼠模型。采用免疫组织化学染色法检测坐骨神经对应背根节神经元Bcl-2、Bax阳性细胞数,免疫印迹法检测背根节Bcl-2、Bax蛋白的表达,TUNEL法检测背根节神经元的凋亡指数。结果:与空白对照组比较,DPN模型组大鼠背根节Bcl-2阳性细胞数明显减少、Bcl-2的表达明显降低,Bax阳性细胞数明显增多、Bax的表达和凋亡指数明显升高(P<0.01)。与DPN模型组比较,远志预防组大鼠背根节Bcl-2阳性细胞数明显增多、Bcl-2的表达明显升高,Bax阳性细胞数明显减少、Bax的表达和凋亡指数明显降低(P<0.01)。结论:远志可通过上调DPN大鼠背根节神经元胞体Bcl-2的表达,减少Bax的表达,抑制背根节神经元凋亡,预防DPN大鼠背根节神经元胞体损伤。
Objective: To investigate the preventive effects of Polygala on sciatic nerve-related neurons injury of diabetic peripheral neuropathy(DPN) rats. Methods: The Wistar rats were randomly divided into 3 groups: blank control group, DPN model group and Polygala prevention group(n=12). The rats in DPN model group and Polygala prevention group were made streptozotocin induced diabetes model. Then the rats in Polygala prevention group were lavaged with Polygala(2.7 g/kg/d)for 6 weeks. The DPN rats' model was established with SNCV<30 m/s as the standard. The immunohistochemical staining was used to detect the number of Bcl-2 and Bax positive cells in sciatic nerve-related dorsal root ganglia; Western Blot to detect Bcl-2 and Bax expression in dorsal root ganglia and TUNEL to detect apoptotic index of neurons in dorsal root ganglia.Results: Compared with blank control group, the Bcl-2 immuno-positive cells and Bcl-2 expression in dorsal root ganglia of DPN rats decreased obviously(P<0.01); while the Bax immuno-positive cells, Bax expression and apoptotic index in dorsal root ganglia increased obviously(P<0.01). Compared with DPN model group, the Bcl-2 immuno-positive cells and Bcl-2 expression in dorsal root ganglia of rats in Polygala prevention group increased obviously(P<0.01); while the Bax immunopositive cells, Bax expression and apoptotic index in dorsal root ganglia of rats in Polygala prevention group decreased obviously(P<0.01). Conclusions: Polygala can inhibit neurons apoptosis in dorsal root ganglia of DPN rats by upregulating Bcl-2 expression and downregulating Bax expression, so has preventive effects on sciatic nerve-related neurons injury.
Objective: To investigate the preventive effects of Polygala on sciatic nerve-related neurons injury of diabetic peripheral neuropathy(DPN) rats. Methods: The Wistar rats were randomly divided into 3 groups: blank control group, DPN model group and Polygala prevention group(n=12). The rats in DPN model group and Polygala prevention group were made streptozotocin induced diabetes model. Then the rats in Polygala prevention group were lavaged with Polygala(2.7 g/kg/d)for 6 weeks. The DPN rats' model was established with SNCV<30 m/s as the standard. The immunohistochemical staining was used to detect the number of Bcl-2 and Bax positive cells in sciatic nerve-related dorsal root ganglia; Western Blot to detect Bcl-2 and Bax expression in dorsal root ganglia and TUNEL to detect apoptotic index of neurons in dorsal root ganglia.Results: Compared with blank control group, the Bcl-2 immuno-positive cells and Bcl-2 expression in dorsal root ganglia of DPN rats decreased obviously(P<0.01); while the Bax immuno-positive cells, Bax expression and apoptotic index in dorsal root ganglia increased obviously(P<0.01). Compared with DPN model group, the Bcl-2 immuno-positive cells and Bcl-2 expression in dorsal root ganglia of rats in Polygala prevention group increased obviously(P<0.01); while the Bax immunopositive cells, Bax expression and apoptotic index in dorsal root ganglia of rats in Polygala prevention group decreased obviously(P<0.01). Conclusions: Polygala can inhibit neurons apoptosis in dorsal root ganglia of DPN rats by upregulating Bcl-2 expression and downregulating Bax expression, so has preventive effects on sciatic nerve-related neurons injury.
引文
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[8]马洪伟,付秀美,付文亮,等.远志对糖尿病周围神经病变大鼠坐骨神经的保护作用[J].中国医科大学学报,2010,39(12):1001-1004.
[2]SOLTESOVA P M,BALLEKOVA J,GAJDOSIKOVA A,et al.A novel carboxymethylated mercaptotriazinoindole inhibitor of aldose reductase interferes with the polyol pathway in streptozotocininduced diabetic rats[J].Physiol Res,2015,64(4):587-591.
[3]GUO C,SHEN J,MENG Z,et al.Neuroprotective effects of polygalacic acid on scopolamine-induced memory deficits in mice[J].Phytomedicine,2016,23(2):149-155.
[4]黄炎,陈逸青,陈勤,等.远志皂苷对Aβ1-40诱导痴呆大鼠海马神经细胞线粒体的保护作用[J].中药药理与临床,2015,31(1):93-97.
[5]SADOSKY A,MARDEKIAN J,PARSONS B,et al.Health care utilization and costs in diabetes relative to the clinical spectrum of painful diabetic peripheral neuropathy[J].Diabetes Res Clin Pract,2015,29(2):212-217.
[6]薛景凤,张炎,张昆.大鼠坐骨神经局部缺血后其神经元胞体超微结构的病理变化[J].解剖学杂志,1996,19(6):502-504.
[7]王永艳,蔡小艳,刘彦刚,等.卵叶远志的研究进展[J].中国医药导报,2016,13(8):33-35.
[8]马洪伟,付秀美,付文亮,等.远志对糖尿病周围神经病变大鼠坐骨神经的保护作用[J].中国医科大学学报,2010,39(12):1001-1004.