西酞普兰对小鼠角膜上皮细胞和泪膜功能的影响
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摘要
目的研究西酞普兰对小鼠泪膜功能和角膜上皮细胞的影响。方法选取36只BABL/c雄性小鼠随机分为A、B、C 3组,A组:空白对照组(不进行任何处理);B组:生理盐水组,以10 mg/kg腹腔注射生理盐水;C组:西酞普兰组,以10 mg/kg腹腔注射西酞普兰。分别在实验第1、4和7天时行泪液分泌试验(SIT)、泪膜破裂时间(BUT)检测,同时于实验第7天进行角膜荧光素染色(FL),取眼球做扫描电镜及HE染色观察角膜上皮损伤情况。结果 (1) 3组不同时间点的SIT、BUT、FL有差异(F=9.749、10.427和6.752,P=0.008、0.019和0.032);(2) 3组的SIT、BUT、FL有差异(F=11.234、14.842和5.337,P=0.002、0.012和0.029);(3) 3组的SIT、BUT、FL变化趋势有差异(F=16.438、11.753和16.431,P=0.000、0.001和0.002)。干预后第7天,3组上皮空泡细胞数比较,差异有统计学意义(F=7.749,P=0.013)。3组微绒毛数量比较差异无统计学意义(F=0.917,P=0.092)。结论西酞普兰会损伤小鼠泪膜功能的稳定性,但对角膜上皮细胞无影响。
Objective To investigate the effect of Citalopram on tear film function and corneal epithelium in mice. Methods A total of 36 BABL/c male mice were randomly divided into group A, group B and group C. Mice in group A received no treatments. Mice in group B received saline(10 ml/kg) via intraperitoneal injection. Mice in group C were intraperitoneally injected with Citalopram(10 mg/kg) on daily basis. Schirmer I test(SIT) and the break-up time(BUT) were carried out on day 1, 4 and 7 post insults. On the 7th day, corneal fluorescein staining(FL) was carried out, and histological analysis of eyeballs of mice were evaluated by scanning electron microscope(SEM) and HE staining. Results SIT, BUT and FL were significantly different on day 1, 4, and 7 in three groups(F = 9.749, 10.427 and 6.752, P = 0.008, 0.019 and 0.032, respectively). SIT, BUT and FL were statistically different among group A, group B and group C(F = 11.234, 14.842 and 5.337, P = 0.002, 0.012 and 0.029, respectively). Sevendays after the intervention, numbers of epithelial cavitation cells in group A, group B and group C were obviously different(F = 7.749, P = 0.013). Mice in group C experienced villi decrease without significant difference among groups(F = 0.917, P = 0.092). Conclusions Citalopram damages the function of tear film while exerts no effect on corneal epithelial tissue in mice model.
Objective To investigate the effect of Citalopram on tear film function and corneal epithelium in mice. Methods A total of 36 BABL/c male mice were randomly divided into group A, group B and group C. Mice in group A received no treatments. Mice in group B received saline(10 ml/kg) via intraperitoneal injection. Mice in group C were intraperitoneally injected with Citalopram(10 mg/kg) on daily basis. Schirmer I test(SIT) and the break-up time(BUT) were carried out on day 1, 4 and 7 post insults. On the 7th day, corneal fluorescein staining(FL) was carried out, and histological analysis of eyeballs of mice were evaluated by scanning electron microscope(SEM) and HE staining. Results SIT, BUT and FL were significantly different on day 1, 4, and 7 in three groups(F = 9.749, 10.427 and 6.752, P = 0.008, 0.019 and 0.032, respectively). SIT, BUT and FL were statistically different among group A, group B and group C(F = 11.234, 14.842 and 5.337, P = 0.002, 0.012 and 0.029, respectively). Sevendays after the intervention, numbers of epithelial cavitation cells in group A, group B and group C were obviously different(F = 7.749, P = 0.013). Mice in group C experienced villi decrease without significant difference among groups(F = 0.917, P = 0.092). Conclusions Citalopram damages the function of tear film while exerts no effect on corneal epithelial tissue in mice model.
引文
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[3]LI M,GONG L,CHAPIN W J,et al.Assessment of vision-related quality of life in dry eye patients[J].Investigative Ophthalmology&Visual Science,2012,53(9):5722-5727.
[4]DEMPSEY L A.Stress-induced depression[J].Nature Immunology,2018,19(2).DOI:10.1038/s 41590-017-0038-2.
[5]MRUGACZ M,OSTROWSKA L,?AZARCZYK-KIREJCZYKJ,et al.Dry eye disease in patients treated with antidepressants[J].Klinika Oczna,2013,115(2):111-114.
[6]赵巍,杨燕宁,袁静,等.眼表分析仪观察人工泪液在抗抑郁药SSRI引起干眼症状前后的应用效果[J].临床眼科杂志,2015,23(1):66-69.
[7]闵幼兰,袁晴,马明洋,等.苯扎溴铵和西酞普兰对小鼠角膜上皮和角膜全层厚度的影响[J].眼科新进展,2018,38(2):101-105.
[8]YU Y,ZOU J,HAN Y,et al.Effects of intravitreal injection of netrin-1 in retinal neovascularization of streptozotocin-induced diabetic rats[J].Drug Design Development&Therapy,2015,9(Issue 1):6363-6377.
[9]SHAO Y,YU Y,LIU Q P,et al.Effects of Honghua preserved amniotic membrane on scar healing in experimental glaucoma surgery[J].Int J Ophthalmol,2014,7(2):226-231.
[10]LIN Z,LIU X,ZHOU T,et al.A mouse dry eye model induced by topical administration of benzalkonium chloride[J].Molecular Vision,2011,17(17):257-264.
[11]PAULY A,BRIGNOLEBAUDOUIN F,LABBE?A,et al.New tools for the evaluation of toxic ocular surface changes in the rat[J].Investigative Ophthalmology&Visual Science,2007,48(12):5473-5483.
[12]NA K S,HAN K,PARK Y G,et al.Depression,stress,quality of life,and dry eye disease in Korean women:a population-based Study[J].Cornea,2015,34(7):733-738.
[13]ACAN D,KURTGOZ P.Influence of selective serotonin reuptake inhibitors on ocular surface[J].Clinical&Experimental Optometry,2017,100(1):83-86.
[14]OUYANG H,XUE Y,LIN Y,et al.WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis[J].Nature,2014,511(7509):358-361.
[15]STERN M E,SCHAUMBURG C S,DANA R,et al.Autoimmunity at the ocular surface:pathogenesis and regulation[J].Mucosal Immunology,2010,3(5):425-442.
[16]CONRADY C D,JOOS Z P,PATEL B C K.Review:the lacrimal gland and its role in dry eye[J].Journal of Ophthalmology,2016,2016:1-11.
[17]BRAUN R J,KING-SMITH P E,BEGLEY C G,et al.Dynamics and function of the tear film in relation to the blink cycle[J].Progress in Retinal&Eye Research,2015,45(10):132-164.
[18]LI L,BRAUN R J,MAKI K L,et al.Tear film dynamics with evaporation,wetting,and time-dependent flux boundary condition on an eye-shaped domain[J].Physics of Fluids,2014,26(5):69-87.
[19]MOON J H,KIM K W,MOON N J.Smartphone use is a risk factor for pediatric dry eye disease according to region and age:a case control study[J].BMC Ophthalmology,2016,16(1):188-194.
[20]YANG C H,ALBIETZ J,HARKIN D G,et al.Impact of oral vitamin D supplementation on the ocular surface in people with dry eye and/or low serum vitamin D[J].Contact Lens&Anterior Eye,2017,41(1):69-76.
[21]AKTAS S,TETIKOGLU M,KO?AK A,et al.Impact of Smoking on the ocular surface,tear function,and tear osmolarity[J].Current Eye Research,2017,42(12):1585-1589.
[22]GIBSON E J,STAPLETON F,WOLFFSOHN J S,et al.Local synthesis of sex hormones:are there consequences for the ocular surface and dry eye[J].British Journal of Ophthalmology,2017,101(12):1596-1603.
[23]AYAKI M,KAWASHIMA M,NEGISHI K,et al.High prevalence of sleep and mood disorders in dry eye patients:survey of 1,000eye clinic visitors[J].Neuropsychiatric Disease&Treatment,2015(11):889-894.
[24]SZAKATS I,SEBESTYEN M,NEMETH J,et al.The role of health anxiety and depressive symptoms in dry eye disease[J].Curr Eye Res,2016,41(8):1044-1049.
[25]HALLAK J A,TIBRREWAL S,JAIN S.Depressive symptoms in patients with dry eye disease:a case-control study using the beck depression inventory[J].Cornea,2015,34(12):1545-1550.