河西走廊三种生态型芦苇土壤中可培养链霉菌及其环境适应特征的研究
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摘要
以河西走廊不同生态型芦苇根际和非根际土壤为样品,采用选择性培养基进行了链霉菌的分离,研究了链霉菌的特性及其与生境的关系。共获得链霉菌355株,其中在2mol L-1Na Cl培养基上分离获得链霉菌70株。16S rRNA基因序列聚类分析表明来源于同一类型土壤中的菌株大致聚在一起。细菌和真菌拮抗性测定表明不同土壤中的链霉菌拮抗性差异显著,沼泽土壤和沙丘土壤中的拮抗性链霉菌多于盐化草甸土壤,根际土壤中拮抗性菌株多于非根际土壤,沙丘土壤中的菌株抗菌活性较弱,盐化土壤中拮抗性菌株种类和数量最少,但其抗菌活性最强。菌株的耐盐性与土壤类型密切相关。这些结果说明土壤的异质性决定着可培养链霉菌的种类和适应特征。本研究获得的细菌和真菌拮抗性链霉菌菌株具有进一步研究的价值。
The culturable Streptomyces strains were isolated from the rhizosphere and bulk soils of three ecotype reeds in the Hexi Corridor desert. A total of 355 Streptomyces strains were obtained,of which 70 Streptomyces strains were obtained from the medium containing 2 mol L-1 NaCl. The cluster analysis based on the 16 S rRNA gene sequences showed that the Streptomyces strains from the same soil type were approximately clustered together. The number of antibacterial strains from the swamp reed soil and dune reed soil were more than that from the saline meadow reed soil,and the antibacterial Streptomyces number from the rhizosphere soils was more than that from the bulk soils. The isolates from the dune reed soils had lower antimicrobial activity than the others. The saline meadow reed soils had the least antagonistic isolates but the strains had the highest antimicrobial activity.The analysis of salt tolerance of the isolated strains showed that the strain tolerance to salt is closely related to the soil types. In summary,the results suggest that the soil heterogeneity and environmental characteristics determine the species and adaptation of the culturable Streptomyces strains. The Streptomyces isolates obtained in this study are worth further studying.
The culturable Streptomyces strains were isolated from the rhizosphere and bulk soils of three ecotype reeds in the Hexi Corridor desert. A total of 355 Streptomyces strains were obtained,of which 70 Streptomyces strains were obtained from the medium containing 2 mol L-1 NaCl. The cluster analysis based on the 16 S rRNA gene sequences showed that the Streptomyces strains from the same soil type were approximately clustered together. The number of antibacterial strains from the swamp reed soil and dune reed soil were more than that from the saline meadow reed soil,and the antibacterial Streptomyces number from the rhizosphere soils was more than that from the bulk soils. The isolates from the dune reed soils had lower antimicrobial activity than the others. The saline meadow reed soils had the least antagonistic isolates but the strains had the highest antimicrobial activity.The analysis of salt tolerance of the isolated strains showed that the strain tolerance to salt is closely related to the soil types. In summary,the results suggest that the soil heterogeneity and environmental characteristics determine the species and adaptation of the culturable Streptomyces strains. The Streptomyces isolates obtained in this study are worth further studying.
引文
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[14]李欣欣,袁树忠,钱兰娟,等.番茄灰霉病菌拮抗放线菌的筛选[J].江苏农业科学,2011,39(5):138-140.
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[16]刘洪升,宋秋华,李凤民.根分泌物对根际矿物营养及根际微生物的效应[J].西北植物学报,2002,22(3):693-702.
[17]王少昆,赵学勇,张铜会,等.科尔沁沙地几种灌木对根际微生物的影响[J].干旱区资源与环境,2012,26(5):140-144.
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[20]Bakker M G,Schlatter D C,Otto-Hanson L,et al. Diffuse symbioses:roles of plant-plant,plant-microbe and microbe-microbe interactions in structuring the soil microbiome[J]. Molecular Ecology,2014,23(6):1571-1583.
[21]Shahzadi N,Basharat A,Shahida H. Growth promotion of Vigna mungo(L.)by Pseudomonas spp. exhibiting auxin production and ACC-deaminase activity[J]. Annals of Microbiology,2012,62(1):411-417.
[22]Karimi E,Sadeghi A,Dahaji P A,et al. Biocontrol activity of salt tolerant Streptomyces isolates against phytopathogens causing root rot of sugar beet[J]. Biocontrol Science&Technology,2012,22(3):333-349.
[23]De Maria S,Rivelli A R,Kuffner M,et al. Interactions between accumulation of trace elements and macronutrients in Salix caprea after inoculation with rhizosphere microorganisms[J]. Chemosphere,2011,84(9):1256-1261.
[24]Thumar J T,Dhulia K,Singh S P. Isolation and partial purification of an antimicrobial agent from halotolerant alkaliphilic Streptomyces aburaviensis Kut-8[J]. World Journal of Microbiology&Biotechnology,2010,26(11):2081-2087.
[25]马玥,来航线,韦小敏,等.新疆荒漠盐碱环境中抗动物病原菌的放线菌筛选与鉴定[J].西北农林科技大学学报(自然科学版),2013,41(3):133-140.
[2]Wilson Z E,Brimble M A. Molecules derived from the extremes of life[J]. Natural Product Reports,2009,26(1):44-71.
[3]张玲,李师翁,陈熙明,等.青藏高原土壤中链霉菌的分离鉴定及其抗菌活性研究[J].冰川冻土,2014,36(2):430-441.
[4]牛世全,阎薇如,李海云,等.河西走廊盐碱土中抗动物病原菌的放线菌筛选与鉴定[J].干旱区资源与环境,2016,30(2):133-138.
[5]Undabarrena A,Beltrametti F,Claverías F P,et al. Exploring the diversity and antimicrobial potential of marine actinobacteria from the Comau Fjord in Northern Patagonia,Chile[J]. Frontiers in Microbiology,2016(7):1135.
[6]Sanghvi G V,Ghevariya D,Gosai S,et al. Isolation and partial purification of erythromycin from alkaliphilic Streptomyces werraensis isolated from Rajkot,India[J]. Biotechnology Reports,2014(1-2):2-7.
[7]牛赟,成彩霞,李小燕,等.河西走廊荒漠区植物生长与水分变化关系分析[J].冰川冻土,2016,38(5):1417-1424.
[8]朱学义,王锁民,张承烈.河西走廊不同生态型芦苇对干旱和盐渍胁迫的响应调节[J].植物生理学通讯,2003,39(4):371-376.
[9]庄瑶,孙一香,王中生,等.芦苇生态型研究进展[J].生态学报,2010,30(8):2173-2181.
[10]王维奇,王纯,仝川,等.闽江河口区盐-淡水梯度下芦苇沼泽土壤有机碳特征[J].湿地科学,2012,10(2):164-169.
[11]李玉强,赵哈林,赵学勇,等.土壤温度和水分对不同类型沙丘土壤呼吸的影响[J].干旱区资源与环境,2006,20(3):154-158.
[12]李广,侯扶江.盐化草甸土壤理化性质的空间格局分析[J].草业学报,2003,12(2):46-53.
[13]李婷,王凯,师瑞芳,等.河西走廊土壤链霉菌的分离及其抗菌活性实验[J].兰州交通大学学报,2016,35(4):127-133.
[14]李欣欣,袁树忠,钱兰娟,等.番茄灰霉病菌拮抗放线菌的筛选[J].江苏农业科学,2011,39(5):138-140.
[15]Zhao F,Qin Y H,Zheng X,et al. Biogeography and adaptive evolution of Streptomyces strains from saline environments[J]. Scientific Reports,2016(6):32718.
[16]刘洪升,宋秋华,李凤民.根分泌物对根际矿物营养及根际微生物的效应[J].西北植物学报,2002,22(3):693-702.
[17]王少昆,赵学勇,张铜会,等.科尔沁沙地几种灌木对根际微生物的影响[J].干旱区资源与环境,2012,26(5):140-144.
[18]Raaijmakers J M,Paulitz C T,Steinberg C,et al. The rhizosphere:a playground and battlefield for soilborne pathogens and beneficial microorganisms[J]. Plant&Soil,2009,321(1-2):341-361.
[19]罗永清,赵学勇,李美霞.植物根系分泌物生态效应及其影响因素研究综述[J].应用生态学报,2012,23(12):3496-3504.
[20]Bakker M G,Schlatter D C,Otto-Hanson L,et al. Diffuse symbioses:roles of plant-plant,plant-microbe and microbe-microbe interactions in structuring the soil microbiome[J]. Molecular Ecology,2014,23(6):1571-1583.
[21]Shahzadi N,Basharat A,Shahida H. Growth promotion of Vigna mungo(L.)by Pseudomonas spp. exhibiting auxin production and ACC-deaminase activity[J]. Annals of Microbiology,2012,62(1):411-417.
[22]Karimi E,Sadeghi A,Dahaji P A,et al. Biocontrol activity of salt tolerant Streptomyces isolates against phytopathogens causing root rot of sugar beet[J]. Biocontrol Science&Technology,2012,22(3):333-349.
[23]De Maria S,Rivelli A R,Kuffner M,et al. Interactions between accumulation of trace elements and macronutrients in Salix caprea after inoculation with rhizosphere microorganisms[J]. Chemosphere,2011,84(9):1256-1261.
[24]Thumar J T,Dhulia K,Singh S P. Isolation and partial purification of an antimicrobial agent from halotolerant alkaliphilic Streptomyces aburaviensis Kut-8[J]. World Journal of Microbiology&Biotechnology,2010,26(11):2081-2087.
[25]马玥,来航线,韦小敏,等.新疆荒漠盐碱环境中抗动物病原菌的放线菌筛选与鉴定[J].西北农林科技大学学报(自然科学版),2013,41(3):133-140.