多氯联苯(Aroclor 1242)胁迫下鲤鱼肝脏组织氧化应激
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摘要
本文在实验室条件下通过静态水质染毒方式在生理生化和转录水平上探究了我国重要经济鱼种鲤鱼(Cyprinus carpioio)对多氯联苯Aroclor 1242的氧化应激:鲤鱼肝脏组织抗氧化酶[超氧化物歧化酶(SuperoxideDismutase,SOD)和过氧化氢酶(Catalase,CAT)]活性、脂质过氧化产物丙二醛(Malonydialdehyde,MDA)含量、相关抗氧化酶编码基因(Cu/Zn-SOD、Mn-SOD、CAT)、应激蛋白HSP70、转录因子Nrf2和芳烃受体AhR2基因表达。结果表明:Aroclor 1242胁迫下,鲤鱼肝脏组织抗氧化酶活性表现出低浓度促进高浓度抑制的趋势;肝脏组织丙二醛含量显著升高,其中390μg/L暴露组鲤鱼肝脏组织MDA含量在第6d高于对照组92.1%(P<0.01),表明肝脏组织发生脂质过氧化,膜系统受到损伤;鲤鱼肝脏组织中Cu/Zn-SOD、Mn-SOD、CAT、HSP70、Nrf2和AhR2编码基因表达出现不同程度下调。本文从酶学和基因转录水平上揭示了Aroclor1242胁迫下鲤鱼肝脏组织细胞的氧化应激。
We investigated the effects of polychlorinated biphenyls Aroclor 1242 on the oxidative stress of Cyprinus carpioio liver under static water exposure in the laboratory. The activities of antioxidant enzymes(SOD, CAT), content of lipid peroxidation products(MDA), expression of genes encoding Cu/Zn-SOD, Mn-SOD, and CAT, heat shock protein 70(HSP70), nuclear factor erythroid 2-related factor 2(Nrf2), and Aryl hydrocarbon receptor 2(AhR2) were analyzed. The results show that Aroclor 1242 changed significantly the SOD and CAT activities in the liver, showing promotion at low concentration and inhibition at high concentration. Aroclor 1242 significantly increased MDA content in the liver tissue. Compared to the control, the MDA content was 77.5% higher in the liver exposed to 390μg/L for three days, indicating that Aroclor 1242 induced strong lipid peroxidation. After exposure to medium-high concentrations of Aroclor 1242(78, 156, and 390μg/L) for 8 days, the expression of antioxidant enzyme encoding gene Cu/Zn-SOD, Mn-SOD, CAT, heat shock protein 70(HSP70), and Nrf2 and AhR2 genes were down-regulated in the liver tissue of the carp. Collectively, this study revealed the influences of Aroclor 1242 on oxidative stress of Cyprinus carpioio liver from the perspectives of enzyme and gene transcription levels.
We investigated the effects of polychlorinated biphenyls Aroclor 1242 on the oxidative stress of Cyprinus carpioio liver under static water exposure in the laboratory. The activities of antioxidant enzymes(SOD, CAT), content of lipid peroxidation products(MDA), expression of genes encoding Cu/Zn-SOD, Mn-SOD, and CAT, heat shock protein 70(HSP70), nuclear factor erythroid 2-related factor 2(Nrf2), and Aryl hydrocarbon receptor 2(AhR2) were analyzed. The results show that Aroclor 1242 changed significantly the SOD and CAT activities in the liver, showing promotion at low concentration and inhibition at high concentration. Aroclor 1242 significantly increased MDA content in the liver tissue. Compared to the control, the MDA content was 77.5% higher in the liver exposed to 390μg/L for three days, indicating that Aroclor 1242 induced strong lipid peroxidation. After exposure to medium-high concentrations of Aroclor 1242(78, 156, and 390μg/L) for 8 days, the expression of antioxidant enzyme encoding gene Cu/Zn-SOD, Mn-SOD, CAT, heat shock protein 70(HSP70), and Nrf2 and AhR2 genes were down-regulated in the liver tissue of the carp. Collectively, this study revealed the influences of Aroclor 1242 on oxidative stress of Cyprinus carpioio liver from the perspectives of enzyme and gene transcription levels.
引文
邢厚娟,2010.阿特拉津和毒死蜱单一及联合暴露对鲤鱼毒理作用的研究.哈尔滨:东北农业大学博士学位论文
刘燕,梁敏,张玄可等,2014.铬暴露对草鱼鳃抗氧化酶活性和脂质过氧化作用的影响.西南大学学报(自然科学版),36(5):46-51
刘仕博,2012.苯并三唑对黄斑蓝子鱼免疫毒性的研究.长春:东北师范大学硕士学位论文
许友卿,梅婕,丁兆坤等,2014.多氯联苯对水生动物抗氧化酶的影响及机理.水产科学,33(4):258-262
李娜,2012.多氯联苯在水生食物链中的生物毒性及富集效应研究.广州:暨南大学硕士学位论文
吴菲力,2016.Aroclor1254诱导稀有鮈鲫氧化应激反应和细胞凋亡相关基因表达的研究.杨凌:西北农林科技大学硕士学位论文
沈骅,王晓蓉,张景飞等,2004.低浓度Zn对幼龄鲫鱼肝脏组织应激蛋白HSP70诱导的影响.农业环境科学学报,23(3):441-443
陈家长,宋超,胡庚东等,2011.微囊藻毒素-LR对罗非鱼肝脏活性氧自由基含量及相关抗氧化酶活性的影响.农业环境科学学报,30(8):1521-1525
奉斌,代其林,王劲,2009.非生物胁迫下植物体内活性氧清除酶系统的研究进展.绵阳师范学院学报,28(11):50-53,77
赵娜,张万起,2005.芳香烃受体及其介导的二噁英毒性研究进展.毒理学杂志,19(1):67-69
赵雪松,任新,段小月等,2016.全氟辛烷磺酸盐暴露对斑马鱼胚胎发育毒性与氧化应激的影响.唐山学院学报,29(6):12-16
胡伟华,何辉,袁勇超等,2017.敌百虫、溴氰菊酯对大鳞副泥鳅仔鱼及多刺裸腹溞的急性毒性实验.淡水渔业,47(1):66-70
姜维丹,2008.肌醇对幼建鲤消化吸收能力和免疫能力的影响.雅安:四川农业大学硕士学位论文
袁立来,2013.典型多环芳烃暴露对稀有鮈鲫药物代谢系统相关基因表达的影响.武汉:华中农业大学博士学位论文
殷健,2014.重金属对斑马鱼的毒性效应及作用机制研究.北京:北京协和医学院博士学位论文
黄健,唐学玺,宫相忠等,2002.低浓度毒物对海洋微藻生长刺激效应的初步研究.应用生态学报,13(11):1516-1518
梅丹,周少奇,2012.垃圾渗滤液对斑马鱼的毒性及抗氧化酶活性的影响.生态环境学报,21(5):902-906
程逸飞,曾鸿鹄,于洋等,2018.磺胺类抗生素对不同革兰氏阴性菌的毒物兴奋效应研究.生态毒理学报,13(2):57-65
虞俊超,2014.多氯联苯和短链氯化石蜡在典型区域环境中的分布及迁移转化研究.济南:山东大学硕士学位论文
薄军,吴世军,李裕红等,2010.苯并[a]芘(BaP)对真鲷细胞色素P450和芳香烃受体基因表达的影响.中山大学学报(自然科学版),49(3):93-97
穆希岩,2015.苯醚甲环唑对斑马鱼毒性及作用机制研究.北京:中国农业大学博士学位论文
Brucka-Jastrzebska E,2010.The effect of aquatic cadmium and lead pollution on lipid peroxidation and superoxide dismutase activity in freshwater fish.Polish Journal of Environmental Studies,19(6):1139-1150
Diletti G,Scortichini G,Abete M C et al,2018.Intake estimates of dioxins and dioxin-like polychlorobiphenyls in the italian general population from the 2013-2016 results of official monitoring plans in food.Science of the Total Environment,627:11-19
Dorantes-Aranda J J,Seger A,Mardones J I et al,2015.Progress in understanding algal bloom-mediated fish kills:the role of superoxide radicals,phycotoxins and fatty acids.PLoS One,10(7):e0133549
Du J L,Cao L P,Liu Y J et al,2016.A study of2,3,7,8-tetrachlorodibenzo-p-dioxin induced liver injury in Jian Carp(Cyprinus carpio var.Jian)using precision-cut liver slices.Bulletin of Environmental Contamination and Toxicology,96(1):55-61
Fattore E,Fanelli R,Dellatte E et al,2008.Assessment of the dietary exposure to non-dioxin-like PCBs of the Italian general population.Chemosphere,73(S1):S278-S283
Giesy J P,Kannan K,1998.Dioxin-like and non-dioxin-like toxic effects of polychlorinated biphenyls(PCBs):implications for risk assessment.Critical Reviews in Toxicology,28(6):511-569
Jiang J,Shi D,Zhou X Q et al,2015.Effects of glutamate on growth,antioxidant capacity,and antioxidant-related signaling molecule expression in primary cultures of fish enterocytes.Fish Physiology and Biochemistry,41(5):1143-1153
Liu N,Wen F L,Li F M,et al,2016.Inhibitory mechanism of phthalate esters on Karenia brevis.Chemosphere,155:498-508
Safe S H,1994.Polychlorinated biphenyls(PCBs):environmental impact,biochemical and toxic responses,and implications for risk assessment.Critical Reviews in Toxicology,24(2):87-149
Stebbing A R D,1982.Hormesis-the stimulation of growth by low levels of inhibitors.Science of the Total Environment,22(3):213-234
Zhang Q,Chen S,Deng F,2014.Effect of heavy metals and organic pollutants on antioxidant enzyme activities in zebra fish by polyacrylamide gel electrophoresis.Journal of Chemical&Pharmaceutical Research,18(9):68-72
刘燕,梁敏,张玄可等,2014.铬暴露对草鱼鳃抗氧化酶活性和脂质过氧化作用的影响.西南大学学报(自然科学版),36(5):46-51
刘仕博,2012.苯并三唑对黄斑蓝子鱼免疫毒性的研究.长春:东北师范大学硕士学位论文
许友卿,梅婕,丁兆坤等,2014.多氯联苯对水生动物抗氧化酶的影响及机理.水产科学,33(4):258-262
李娜,2012.多氯联苯在水生食物链中的生物毒性及富集效应研究.广州:暨南大学硕士学位论文
吴菲力,2016.Aroclor1254诱导稀有鮈鲫氧化应激反应和细胞凋亡相关基因表达的研究.杨凌:西北农林科技大学硕士学位论文
沈骅,王晓蓉,张景飞等,2004.低浓度Zn对幼龄鲫鱼肝脏组织应激蛋白HSP70诱导的影响.农业环境科学学报,23(3):441-443
陈家长,宋超,胡庚东等,2011.微囊藻毒素-LR对罗非鱼肝脏活性氧自由基含量及相关抗氧化酶活性的影响.农业环境科学学报,30(8):1521-1525
奉斌,代其林,王劲,2009.非生物胁迫下植物体内活性氧清除酶系统的研究进展.绵阳师范学院学报,28(11):50-53,77
赵娜,张万起,2005.芳香烃受体及其介导的二噁英毒性研究进展.毒理学杂志,19(1):67-69
赵雪松,任新,段小月等,2016.全氟辛烷磺酸盐暴露对斑马鱼胚胎发育毒性与氧化应激的影响.唐山学院学报,29(6):12-16
胡伟华,何辉,袁勇超等,2017.敌百虫、溴氰菊酯对大鳞副泥鳅仔鱼及多刺裸腹溞的急性毒性实验.淡水渔业,47(1):66-70
姜维丹,2008.肌醇对幼建鲤消化吸收能力和免疫能力的影响.雅安:四川农业大学硕士学位论文
袁立来,2013.典型多环芳烃暴露对稀有鮈鲫药物代谢系统相关基因表达的影响.武汉:华中农业大学博士学位论文
殷健,2014.重金属对斑马鱼的毒性效应及作用机制研究.北京:北京协和医学院博士学位论文
黄健,唐学玺,宫相忠等,2002.低浓度毒物对海洋微藻生长刺激效应的初步研究.应用生态学报,13(11):1516-1518
梅丹,周少奇,2012.垃圾渗滤液对斑马鱼的毒性及抗氧化酶活性的影响.生态环境学报,21(5):902-906
程逸飞,曾鸿鹄,于洋等,2018.磺胺类抗生素对不同革兰氏阴性菌的毒物兴奋效应研究.生态毒理学报,13(2):57-65
虞俊超,2014.多氯联苯和短链氯化石蜡在典型区域环境中的分布及迁移转化研究.济南:山东大学硕士学位论文
薄军,吴世军,李裕红等,2010.苯并[a]芘(BaP)对真鲷细胞色素P450和芳香烃受体基因表达的影响.中山大学学报(自然科学版),49(3):93-97
穆希岩,2015.苯醚甲环唑对斑马鱼毒性及作用机制研究.北京:中国农业大学博士学位论文
Brucka-Jastrzebska E,2010.The effect of aquatic cadmium and lead pollution on lipid peroxidation and superoxide dismutase activity in freshwater fish.Polish Journal of Environmental Studies,19(6):1139-1150
Diletti G,Scortichini G,Abete M C et al,2018.Intake estimates of dioxins and dioxin-like polychlorobiphenyls in the italian general population from the 2013-2016 results of official monitoring plans in food.Science of the Total Environment,627:11-19
Dorantes-Aranda J J,Seger A,Mardones J I et al,2015.Progress in understanding algal bloom-mediated fish kills:the role of superoxide radicals,phycotoxins and fatty acids.PLoS One,10(7):e0133549
Du J L,Cao L P,Liu Y J et al,2016.A study of2,3,7,8-tetrachlorodibenzo-p-dioxin induced liver injury in Jian Carp(Cyprinus carpio var.Jian)using precision-cut liver slices.Bulletin of Environmental Contamination and Toxicology,96(1):55-61
Fattore E,Fanelli R,Dellatte E et al,2008.Assessment of the dietary exposure to non-dioxin-like PCBs of the Italian general population.Chemosphere,73(S1):S278-S283
Giesy J P,Kannan K,1998.Dioxin-like and non-dioxin-like toxic effects of polychlorinated biphenyls(PCBs):implications for risk assessment.Critical Reviews in Toxicology,28(6):511-569
Jiang J,Shi D,Zhou X Q et al,2015.Effects of glutamate on growth,antioxidant capacity,and antioxidant-related signaling molecule expression in primary cultures of fish enterocytes.Fish Physiology and Biochemistry,41(5):1143-1153
Liu N,Wen F L,Li F M,et al,2016.Inhibitory mechanism of phthalate esters on Karenia brevis.Chemosphere,155:498-508
Safe S H,1994.Polychlorinated biphenyls(PCBs):environmental impact,biochemical and toxic responses,and implications for risk assessment.Critical Reviews in Toxicology,24(2):87-149
Stebbing A R D,1982.Hormesis-the stimulation of growth by low levels of inhibitors.Science of the Total Environment,22(3):213-234
Zhang Q,Chen S,Deng F,2014.Effect of heavy metals and organic pollutants on antioxidant enzyme activities in zebra fish by polyacrylamide gel electrophoresis.Journal of Chemical&Pharmaceutical Research,18(9):68-72