正交设计优选合爽舒颗粒提取工艺
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摘要
目的优化合爽舒颗粒的提取工艺。方法采用正交设计,以欧前胡素含量和浸膏得率为评价指标,考察含乙醇浓度、乙醇用量、提取时间、提取次数对醇提工艺的影响;以橙皮苷含量和浸膏得率为评价指标,考察提取次数、提取时间、水的用量对水提工艺的影响;采用HPLC测定欧前胡素和橙皮苷的含量。结果醇提最佳工艺为:加8倍药材量的70%乙醇提取3次,每次1.5 h;水提最佳工艺为:加18倍药材量的水提取煎煮3次,每次1.5 h。结论该提取工艺稳定、可行,为合爽舒颗粒的合理开发提供参考。
Objective To optimize the extraction technology of Heshuangshu granule. Methods With ammidin content and dry extract rate as the indexes,orthogonal test was adopted to optimize the alcohol extraction process including extraction times,extraction time,the concentration and consumption of ethanol as factors. With dry extract rate and aurantiamarin amount as the indexes,orthogonal test was adopted to investigate the effects of the amount of water,boiling time and decocting times on the water extraction process. Contents of ammidin and aurantiamarin were determined by HPLC. Results Optimum alcohol extraction process was determined as follows: solid-liquid ratio 1: 8,70% ethanol,refluxing and extracting for 1. 5 h,3 times. Optimum water extraction process was determined as follows: decocted 3 times with 18 times the amount water,1. 5 h each time. Conclusion The optimized processes are stable and efficient,which could provide a reference for rational development of Heshuangshu granule.
Objective To optimize the extraction technology of Heshuangshu granule. Methods With ammidin content and dry extract rate as the indexes,orthogonal test was adopted to optimize the alcohol extraction process including extraction times,extraction time,the concentration and consumption of ethanol as factors. With dry extract rate and aurantiamarin amount as the indexes,orthogonal test was adopted to investigate the effects of the amount of water,boiling time and decocting times on the water extraction process. Contents of ammidin and aurantiamarin were determined by HPLC. Results Optimum alcohol extraction process was determined as follows: solid-liquid ratio 1: 8,70% ethanol,refluxing and extracting for 1. 5 h,3 times. Optimum water extraction process was determined as follows: decocted 3 times with 18 times the amount water,1. 5 h each time. Conclusion The optimized processes are stable and efficient,which could provide a reference for rational development of Heshuangshu granule.
引文
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[2]舒翔,叶小春,陈军,等.高效液相色谱法同时测定白芷中欧前胡素和异欧前胡素的含量[J].中国医院药学杂志,2017,33(5):427-429.
[3]李昌勤,彭琳娜,姚辰,等.加热处理对白芷中欧前胡素、异欧前胡素稳定性及酪氨酸酶活性的影响[J].中国医院药学杂志,2017,18(5):407-412.
[4]袁子民,王静,贾天柱,等.白芷酒炖前后4种香豆素类成分的含量测定及相互转化机制研究[J].中国中药杂志,2017,21(16):3032-3035.
[5]王旭,侯豹,蔡维维,等.王不留行黄酮苷的分离纯化及对细胞增殖活性的影响[J].华西药学杂志,2017,29(2):316-321.
[6]汪晶晶,任红立,武洪志,等.中药王不留行的化学成分及药理作用研究进展[J].黑龙江畜牧兽医,2017,17(7):101-103.
[7]齐梁煜,黄月维,黄春阳,等.王不留行总黄酮对离体蛙心心肌收缩力和心率的影响[J].中国医院药学杂志,2016,36(10):802-806.
[8]徐玉玲,伍利华,李鹏程,等.基于橙皮苷临床利用量的青皮品质评价研究[J].西北植物学报,2016,31(22):4009-4015.
[9]邱蓉丽,吴玉兰,乐巍.陈皮、青皮中4种黄酮成分的比较研究[J].中成药,2015,30(1):149-153.
[10]王桂红,张远远,肖伊,等.不同产地个青皮高效液相色谱指纹图谱研究[J].医药导报,2011,30(4):429-431.
[11]王春林,杨伟明,邬江华.遵义市16 606例女性乳腺增生症普查结果分析[J].遵义医学院学报,2012,35(3):244-246.
[12]蒋国元,申霞,袁西伟.钼靶表现为肿块样改变的乳腺增生症的影像诊断[J].遵义医学院学报,2011,34(4):377-379.