HPLC法测定丹参地上部分中丹酚酸B和迷迭香酸的含量
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摘要
建立了使用HPLC同时测定丹参地上部分中丹酚酸B和迷迭香酸含量的分析方法。在流动相甲醇(A)-0.8%冰乙酸水溶液(B)梯度洗脱,流速1 m L/min,柱温35℃,检测波长250 nm色谱条件下测定结果表明,丹酚酸B和迷迭香酸的质量浓度分别在14.0～700.0μg/m L (r=1.000 0)和12～600μg/m L (r=0.999 6)的线性范围内,有良好的线性关系;丹酚酸B和迷迭香酸的检出限LOD (S/N=3)分别为0.2,0.3μg/m L,定量限LOQ (S/N=10)分别为1.0,1.0μg/m L。丹酚酸B和迷迭香酸的平均加样回收率分别为99.16%(RSD=0.735%)和101.11%(RSD=2.11%)。该方法操作简便、分析效果好、灵敏度高,具有良好的重复性和回收率,可同时测得丹参地上部分中水溶性成分丹酚酸B和迷迭香酸的含量。
To develop a HPLC method for investigate the determination of the content of salvianolic acid B and rosmarinic acid in the aerial part of Salvia miltiorrhiza. Gradient elution in mobile phase methanol(A)-0.8% aqueous glacial acetic acid(B),flow rate 1 m L/min,column temperature 35 ℃,detection wavelength 250 nm. The results showed that a good linearIity was obtained over 14.0～700.0 μg/m L(r=1.000 0) for salvianolic acid B,12～600 μg/m L(r=0.999 6) for rosmarinic acid,respectively. The average recoveries of salvianolic acid B and rosmarinic acid were 99.16%(RSD=0.735%) and 101.11%(RSD=2.11%),respectively. The HPLC method was simple and easy to perform,and the analytical effect is good. The content of two water-soluble components,salvianolic acid B and rosmarinic acid,can be measured simultaneously.
To develop a HPLC method for investigate the determination of the content of salvianolic acid B and rosmarinic acid in the aerial part of Salvia miltiorrhiza. Gradient elution in mobile phase methanol(A)-0.8% aqueous glacial acetic acid(B),flow rate 1 m L/min,column temperature 35 ℃,detection wavelength 250 nm. The results showed that a good linearIity was obtained over 14.0～700.0 μg/m L(r=1.000 0) for salvianolic acid B,12～600 μg/m L(r=0.999 6) for rosmarinic acid,respectively. The average recoveries of salvianolic acid B and rosmarinic acid were 99.16%(RSD=0.735%) and 101.11%(RSD=2.11%),respectively. The HPLC method was simple and easy to perform,and the analytical effect is good. The content of two water-soluble components,salvianolic acid B and rosmarinic acid,can be measured simultaneously.
引文
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[2]肖禾,宋民宪,赖娟.国家药品标准中丹参的质量控制情况[J].中国药事,2006,20(1):57-59.
[3]段金廒,宿树兰,郭盛,等.中药资源产业化过程废弃物的产生及其利用策略与资源化模式[J].中草药,2018, 20(3):298-300.
[4]孙国平,徐亚莉,司亚楠.丹参叶的研究进展[J].医药前沿,2012(2):128-129.
[5]沙秀秀,宿树兰,沈飞,等.不同生长期丹参茎叶及花序中丹酚酸类化学成分的分布与积累动态分析评价[J].中草药,2015,46(2):3 414-3 419.
[6]李欣,薛治浦,朱文学.丹参不同部位总酚酸和总黄酮含量分析及其抗氧化活性研究[J].食品科学,2011,32(3):108-111.
[7] Matkowski A, Sylwia Zielinska, Jan Oszmianski, et al.Antioxidant activity of extracts from leaves and roots of Salvia miltiorrhiza Bunge, S. przewalskii Maxim and S. verticillata L.[J]. Bioresour Technol, 2008(16):7 892-7 896.
[8]张寒,贾敏,杨颖.丹参地上部分抗脑缺血活性部位筛选[J].中药药理与临床,2013,29(3):133-136.
[9]刁保忠,靳维荣,张文周.正交试验优选丹参茎叶中丹参素和丹酚酸B的提取工艺研究[J].中华中医药杂志,2013, 28 0:2 717-2 720.
[10]杨志霞,林谦,马利.丹参对心血管疾病药理作用的文献研究[J].世界中西医结合杂志,2012, 7(2):93-96.
[11]李霞,杨阳,黄豆豆,等.甘西鼠尾草药材和提取物中迷迭香酸、丹酚酸B的含量测定[J].中国医院药学杂志,2014,34(19):1 634-1 638.
[12]王招玉,徐依萍,焦蓉,等.HPLC法同时测定丹参中原儿茶醛、迷迭香酸、丹酚酸A的含量[J].中国药师,2014, 17(9):1 473-1 475.