雾霾空气中细菌特征及对健康的潜在影响
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摘要
论文从群落结构、浓度变化及粒径分布的角度论述了雾霾天气对空气细菌特征的影响,并结合了雾霾天气相关疾病的发病率,综合评价了雾霾天气空气病原菌导致的人群潜在健康风险的变化,最后提出了以前研究存在的不足及未来的发展趋势。综合研究结果,雾霾天气对空气细菌群落结构、浓度变化及粒径分布的影响,研究人员在不同城市得出的研究结果不同,可能由样点的时空环境、气象因素、雾霾程度及采集、检验、鉴定空气细菌方法等多种因素的差异引起。雾霾空气中已发现病原细菌均为条件致病菌,在空气中含量很低,但雾霾天气下部分病原菌的相对丰度增加,致病力会显著增强。此外,高浓度的细颗粒物和化学污染物可损伤皮肤黏膜屏障,打破呼吸道和皮肤的微生态平衡,为病原菌侵入创造较好的机会。两者的协同作用,显著增加了雾霾天气空气中病原菌的健康风险。
This study explored the impacts of atmospheric haze on airborne bacteria in terms of bacterial community structure, as well as concentrations and size distributions. We evaluated changes in the potential risks to human health caused by airborne pathogens, based on the morbidity of atmospheric haze-related diseases. Finally, we addressed the shortcomings of previous studies and proposed future trends for related research. The dynamics of bacterial community structure, and concentration and size distribution of bacterial communities in atmospheric haze—on which domestic studies have focused—have not shown consistent patterns to date. These uncertainties may be influenced by collection and detection methods, spatial and temporal disparities, meteorological factors, haze levels, etc. Pathogenic bacteria found on hazy days were opportunistic pathogens with low concentrations. However, the relative abundance and virulence of some bacterial species could significantly increase under hazy conditions. In addition, the high concentration of fine particulate matter with chemical pollutants could damage the skin or the mucous membrane barrier, break the micro-ecological balance of the respiratory tract and skin, and create opportunities for pathogen invasion. The synergistic effects of particulate matter and chemical pollutants have significantly contributed to the health risks from pathogenic bacteria in atmospheric haze.
This study explored the impacts of atmospheric haze on airborne bacteria in terms of bacterial community structure, as well as concentrations and size distributions. We evaluated changes in the potential risks to human health caused by airborne pathogens, based on the morbidity of atmospheric haze-related diseases. Finally, we addressed the shortcomings of previous studies and proposed future trends for related research. The dynamics of bacterial community structure, and concentration and size distribution of bacterial communities in atmospheric haze—on which domestic studies have focused—have not shown consistent patterns to date. These uncertainties may be influenced by collection and detection methods, spatial and temporal disparities, meteorological factors, haze levels, etc. Pathogenic bacteria found on hazy days were opportunistic pathogens with low concentrations. However, the relative abundance and virulence of some bacterial species could significantly increase under hazy conditions. In addition, the high concentration of fine particulate matter with chemical pollutants could damage the skin or the mucous membrane barrier, break the micro-ecological balance of the respiratory tract and skin, and create opportunities for pathogen invasion. The synergistic effects of particulate matter and chemical pollutants have significantly contributed to the health risks from pathogenic bacteria in atmospheric haze.
引文
[1] 徐羽贞.微生物气溶胶静电收集技术研究[D].杭州:浙江大学,2015.
[2] Cao C,Jiang W J,Wang B Y,Fang J H,Lang J D,Tian G,Jiang J K,Zhu T F.Inhalable microorganisms in Beijing′s PM2.5 and PM10 pollutants during a severe smog event.Environmental Science & Technology,2014,48(3):1499- 1507.
[3] 马曼曼,甄毓,米铁柱,祁建华,邵聪聪,冯文荣.青岛冬季霾天不同粒径生物气溶胶中细菌群落特征研究.中国环境科学,2017,37(8):2855- 2865.
[4] Smets W,Moretti S,Denys S,Lebeer S.Airborne bacteria in the atmosphere:presence,purpose,and potential.Atmospheric Environment,2016,139:214- 221.
[5] 方治国,郝翠梅,姚文冲,欧阳志云.空气微生物群落解析方法:从培养到非培养.生态学报,2016,36(14):4244- 4253.
[6] 路凤,金银龙,程义斌.军团菌病的流行概况.国外医学卫生学分册,2008,35(2):78- 83.
[7] 李明远,徐志凯.医学微生物学(第三版).北京:人民卫生出版社,2015:117- 206.
[8] 李兰娟.感染微生态学(第二版).北京:人民卫生出版社,2012:79- 97,196- 219,264- 299.
[9] Gao J F,Fan X Y,Li H Y,Pan K L.Airborne bacterial communities of PM2.5 in Beijing-Tianjin-Hebei Megalopolis,China as revealed by illumina miseq sequencing:a case study.Aerosol and Air Quality Research,2016,17(3):788- 798.
[10] Smets W,Moretti S,Lebeer S.Study of airborne bacteria and their relation to air pollutants//Proceedings of the 9th International Conference on Urban Regeneration and Sustainability.Siena,Italy:WIT Press,2014:1449- 1458.
[11] Zhen Q,Deng Y,Wang Y Q,Wang X K,Zhang H X,Sun X,Ouyang Z Y.Meteorological factors had more impact on airborne bacterial communities than air pollutants.Science of the Total Environment,2017,601- 602:703- 712.
[12] Tong Y Y,Lighthart B.Solar radiation has a lethal effect on natural populations of culturable outdoor atmospheric bacteria.Atmospheric Environment,1997,31(6):897- 900.
[13] Alghamdi M A,Shamy M,Redal M A,Khoder M,Awad A H,Elserougy S.Microorganisms associated particulate matter:a preliminary study.Science of the Total Environment,2014,479- 480:109- 116.
[14] Li Y P,Fu H L,Wang W,Liu J,Meng Q L,Wang W K.Characteristics of bacterial and fungal aerosols during the autumn haze days in Xi′an,China.Atmospheric Environment,2015,122:439- 447.
[15] 王伟,付红蕾,王廷路,宋颖,扶娟,项麦祺,李彦鹏.西安市秋季灰霾天气微生物气溶胶的特性研究.环境科学学报,2016,36(1):279- 288.
[16] 司恒波.微生物气溶胶与大气细颗粒物的相关性研究[D].西安:长安大学,2014.
[17] 胡亚东,马安周,吕鹏翼,张扬,庄国强.北京和保定地区大气细颗粒物中可培养细菌的种群特征.环境科学,2017,38(4):1327- 1339.
[18] Xu C H,Wei M,Chen J M,Wang X F,Zhu C,Li J R,Zheng L L,Sui G D,Li W J,Wang W X,Zhang Q Z,Mellouki A.Bacterial characterization in ambient submicron particles during severe haze episodes at Ji′nan,China.Science of the Total Environment,2017,580:188- 196.
[19] 武丽婧.青岛及黄海生物气溶胶中微生物群落多样性研究[D].青岛:中国海洋大学,2014.
[20] Wei K,Zou Z L,Zheng Y H,Li J,Shen F X,Wu C Y,Wu Y S,Hu M,Yao M S.Ambient bioaerosol particle dynamics observed during haze and sunny days in Beijing.Science of the Total Environment,2016,550:751- 759.
[21] 邵聪聪,甄毓,米铁柱,马曼曼,冯文荣.青岛冬季霾天与非霾天大气细菌群落结构特征研究.城市环境与城市生态,2016,29(3):7- 13.
[22] 袁兴程,杜娟.徐州市城郊雾霾天气中PM2.5物化及微生物特征分析.广东化工,2017,44(1):18- 18,38- 38.
[23] Dong L J,Qi J H,Shao C C,Zhong X,Gao D M,Cao W W,Gao J W,Bai R,Long G Y,Chu C C.Concentration and size distribution of total airborne microbes in hazy and foggy weather.Science of the Total Environment,2016,541:1011- 1018.
[24] Gao M,Jia R Z,Qiu T L,Han M L,Song Y,Wang X M.Seasonal size distribution of airborne culturable bacteria and fungi and preliminary estimation of their deposition in human lungs during non-haze and haze days.Atmospheric Environment,2015,118:203- 210.
[25] 高敏,仇天雷,贾瑞志,韩梅琳,宋渊,王旭明.北京雾霾天气生物气溶胶浓度和粒径特征.环境科学,2014,35(12):4415- 4421.
[26] 付红蕾.西安市不同天气可培养微生物气溶胶的分布特性研究[D].西安:长安大学,2016.
[27] 李婉欣,路瑞,谢铮胜,王金龙,范春兰,刘鹏霞,李彦鹏.西安市秋冬季不同空气质量下可培养微生物气溶胶浓度和粒径分布.环境科学,2017,38(11):4494- 4500.
[28] 路瑞,李婉欣,宋颖,谢铮胜,李彦鹏.西安市不同天气下可培养微生物气溶胶浓度变化特征.环境科学研究,2017,30(7):1012- 1019.
[29] 胡凌飞,张柯,王洪宝,李娜,王洁,杨文慧,殷喆,焦周光,温占波,李劲松.北京雾霾天大气颗粒物中微生物气溶胶的浓度及粒谱特征.环境科学,2015,36(9):3144- 3149.
[30] Gao M,Yan X,Qiu T L,Han M L,Wang X M.Variation of correlations between factors and culturable airborne bacteria and fungi.Atmospheric Environment,2016,128:10- 19.
[31] Gao M,Qiu T L,Jia R Z,Han M L,Song Y,Wang X M.Concentration and size distribution of viable bioaerosols during non-haze and haze days in Beijing.Environmental Science and Pollution Research,2015,22(6):4359- 4368.
[32] 韩晨,谢绵测,祁建华,张文倩,李先国,张大海.青岛市不同空气质量下可培养生物气溶胶分布特征及影响因素.环境科学研究,2016,29(9):1264- 1271.
[33] 薛林贵,姜金融,Famous E.城市空气微生物的监测及研究进展.环境工程,2017,35(3):152- 157,162- 162.
[34] 于玺华.现代空气微生物学.北京:人民军医出版社,2002.
[35] 方治国,欧阳志云,胡利锋,王效科,林学强.室外空气细菌群落特征研究进展.应用与环境生物学报,2005,11(1):123- 128.
[36] Shaffer B T,Lighthart B.Survey of culturable airborne bacteria at four diverse locations in Oregon:urban,rural,forest,and coastal.Microbial Ecology,1997,34(3):167- 177.
[37] Fang Z G,Ouyang Z Y,Zheng H,Wang X K.Concentration and size distribution of culturable airborne microorganisms in outdoor environments in Beijing,China.Aerosol Science and Technology,2008,42(5):325- 334.
[38] Li M F,Qi J H,Zhang H D,Huang S,Li L,Gao D M.Concentration and size distribution of bioaerosols in an outdoor environment in the Qingdao coastal region.Science of the Total Environment,2011,409(19):3812- 3819.
[39] Yan D,Zhang T,Su J,Zhao L L,Wang H,Fang X M,Zhang Y Q,Liu H Y,Yu L Y.Diversity and composition of airborne fungal community associated with particulate matters in beijing during haze and Non-haze days.Frontiers in Microbiology,2016,7:487.
[40] 姚文冲.基于高通量测序的南方典型旅游城市空气细菌群落特征研究[D].杭州:浙江工商大学,2017.
[41] 李林佳.青岛市不同季节雾霾期与非雾霾期空气微生物多样性比较研究[D].青岛:青岛理工大学,2015.
[42] 陆德源.医学微生物学(第五版).北京:人民卫生出版社,2001:69- 86.
[43] Kaushik R,Balasubramanian R.Assessment of bacterial pathogens in fresh rainwater and airborne particulate matter using Real-Time PCR.Atmospheric Environment,2012,46:131- 139.
[44] 周蓉,朱卫民.肺炎克雷伯菌分子流行病学及耐药机制研究进展.国外医药抗生素分册,2012,33(1):1- 5,42- 42.
[45] 任南,文细毛,吴安华.2014年全国医院感染横断面调查报告.中国感染控制杂志,2016,15(2):83- 87.
[46] U.S.Environmental Protection Agency.Product performance test guidelines:OCSPP 810.2500:air sanitizers—efficacy data recommendations.(2012).https://www.regulations.gov/contentStreamer?documentId=EPA-HQ-OPPT- 2009-0150-0025&contentType=pdf.
[47] Fang Z G,Ouyang Z Y,Zheng H,Wang X K,Hu L F.Culturable airborne bacteria in outdoor environments in Beijing,China.Microbial Ecology,2007,54(3):487- 496.
[48] Woo A C,Brar M S,Chan Y,Lau M C Y,Leung F C C,Scott J A,Vrijmoed L L P,Zawar-Reza P Z,Pointing S B.Temporal variation in airborne microbial populations and microbially-derived allergens in a tropical urban landscape.Atmospheric Environment,2013,74:291- 300.
[49] 陈佰义,何礼贤,胡必杰,倪语星,邱海波,石岩,施毅,王辉,王明贵,杨毅,张菁,俞云松.中国鲍曼不动杆菌感染诊治与防控专家共识.中华医学杂志,2012,92(2):76- 85.
[50] Fahlgren C,Hagstr?m ?,Nilsson D,Zweifel U L.Annual variations in the diversity,viability,and origin of airborne bacteria.Applied and Environmental Microbiology,2010,76(9):3015- 3025.
[51] 高秀娟,张晓丽,刘骙骙,张瑜.某医院临床分离阴沟肠杆菌分布及耐药性变迁.中国消毒学杂志,2017,34(4):350- 352.
[52] 姚雪华,杨跃杰,王文豪.破伤风患者预后的影响因素分析.中国实用医药,2016,11(17):90- 92.
[53] 蒋琪霞,王建东,徐元玲,彭青,周昕,李洋,黄秀玲.慢性伤口感染常见病原菌及其干预效果研究.护理学杂志,2015,30(12):19- 23.
[54] 王新华.住院患者1304例呼吸道感染原体检测结果分析.实用医技杂志,2015,22(2):167- 168.
[55] 刘丹,赵雨佳,王祎星,张慧峰,高慧明,周辉.雾霾和沙尘暴天气细颗粒物对人角质细胞促炎症作用和过敏反应的影响.毒理学杂志,2017,31(2):102- 108.
[56] 邱勇,张志红.大气细颗粒物免疫毒性研究进展.环境与健康杂志,2011,28(12):1117- 1120.
[57] Mancebo S E,Wang S Q.Recognizing the impact of ambient air pollution on skin health.Journal of the European Academy of Dermatology and Venereology,2015,29(12):2326- 2332.
[58] 吴迪,李潇,卢永波.雾霾对皮肤屏障功能的毒性作用研究//中国毒理学会,湖北省科学技术协会.中国毒理学会第七次全国毒理学大会暨第八届湖北科技论坛论文集.武汉:中国毒理学会,湖北省科学技术协会,2015:1.
[59] 肖纯凌,韩秀珍,魏德洲,孙文娟,叶丽杰,席淑华,崔金山.大气污染对人体免疫功能及微生态的影响.中国微生态学杂志,2002,14(1):12- 13.
[60] 肖纯凌.大气污染与呼吸道微生态.沈阳医学院学报,2006,8(1):1- 3.
[61] 张瑜,肖纯凌.呼吸道微生态在大气污染中的改变与调节的研究进展.中国当代医药,2017,24(12):12- 15.
[62] 仲崇山.雾霾诱发呼吸道感染者增多.新华日报,2010- 10- 12(A06).
[63] 李惠,赵学良,程芳,王辉,金小力.雾霾天气对荨麻疹就诊量和病情的影响.皮肤性病诊疗学杂志,2017,24(2):112- 115.
[64] Hussey S J K,Purves J,Allcock N,Fernandes V E,Monks P S,Ketley J M,Andrew P W,Morrissey J A.Air pollution alters Staphylococcus aureus and Streptococcus pneumoniae biofilms,antibiotic tolerance and colonisation.Environmental Microbiology,2017,19(5):1868- 1880.
[65] 高丽娜.冬季雾霾天气手术室空气净化效果观察.中国消毒学杂志,2016,33(12):1227- 1228.
[2] Cao C,Jiang W J,Wang B Y,Fang J H,Lang J D,Tian G,Jiang J K,Zhu T F.Inhalable microorganisms in Beijing′s PM2.5 and PM10 pollutants during a severe smog event.Environmental Science & Technology,2014,48(3):1499- 1507.
[3] 马曼曼,甄毓,米铁柱,祁建华,邵聪聪,冯文荣.青岛冬季霾天不同粒径生物气溶胶中细菌群落特征研究.中国环境科学,2017,37(8):2855- 2865.
[4] Smets W,Moretti S,Denys S,Lebeer S.Airborne bacteria in the atmosphere:presence,purpose,and potential.Atmospheric Environment,2016,139:214- 221.
[5] 方治国,郝翠梅,姚文冲,欧阳志云.空气微生物群落解析方法:从培养到非培养.生态学报,2016,36(14):4244- 4253.
[6] 路凤,金银龙,程义斌.军团菌病的流行概况.国外医学卫生学分册,2008,35(2):78- 83.
[7] 李明远,徐志凯.医学微生物学(第三版).北京:人民卫生出版社,2015:117- 206.
[8] 李兰娟.感染微生态学(第二版).北京:人民卫生出版社,2012:79- 97,196- 219,264- 299.
[9] Gao J F,Fan X Y,Li H Y,Pan K L.Airborne bacterial communities of PM2.5 in Beijing-Tianjin-Hebei Megalopolis,China as revealed by illumina miseq sequencing:a case study.Aerosol and Air Quality Research,2016,17(3):788- 798.
[10] Smets W,Moretti S,Lebeer S.Study of airborne bacteria and their relation to air pollutants//Proceedings of the 9th International Conference on Urban Regeneration and Sustainability.Siena,Italy:WIT Press,2014:1449- 1458.
[11] Zhen Q,Deng Y,Wang Y Q,Wang X K,Zhang H X,Sun X,Ouyang Z Y.Meteorological factors had more impact on airborne bacterial communities than air pollutants.Science of the Total Environment,2017,601- 602:703- 712.
[12] Tong Y Y,Lighthart B.Solar radiation has a lethal effect on natural populations of culturable outdoor atmospheric bacteria.Atmospheric Environment,1997,31(6):897- 900.
[13] Alghamdi M A,Shamy M,Redal M A,Khoder M,Awad A H,Elserougy S.Microorganisms associated particulate matter:a preliminary study.Science of the Total Environment,2014,479- 480:109- 116.
[14] Li Y P,Fu H L,Wang W,Liu J,Meng Q L,Wang W K.Characteristics of bacterial and fungal aerosols during the autumn haze days in Xi′an,China.Atmospheric Environment,2015,122:439- 447.
[15] 王伟,付红蕾,王廷路,宋颖,扶娟,项麦祺,李彦鹏.西安市秋季灰霾天气微生物气溶胶的特性研究.环境科学学报,2016,36(1):279- 288.
[16] 司恒波.微生物气溶胶与大气细颗粒物的相关性研究[D].西安:长安大学,2014.
[17] 胡亚东,马安周,吕鹏翼,张扬,庄国强.北京和保定地区大气细颗粒物中可培养细菌的种群特征.环境科学,2017,38(4):1327- 1339.
[18] Xu C H,Wei M,Chen J M,Wang X F,Zhu C,Li J R,Zheng L L,Sui G D,Li W J,Wang W X,Zhang Q Z,Mellouki A.Bacterial characterization in ambient submicron particles during severe haze episodes at Ji′nan,China.Science of the Total Environment,2017,580:188- 196.
[19] 武丽婧.青岛及黄海生物气溶胶中微生物群落多样性研究[D].青岛:中国海洋大学,2014.
[20] Wei K,Zou Z L,Zheng Y H,Li J,Shen F X,Wu C Y,Wu Y S,Hu M,Yao M S.Ambient bioaerosol particle dynamics observed during haze and sunny days in Beijing.Science of the Total Environment,2016,550:751- 759.
[21] 邵聪聪,甄毓,米铁柱,马曼曼,冯文荣.青岛冬季霾天与非霾天大气细菌群落结构特征研究.城市环境与城市生态,2016,29(3):7- 13.
[22] 袁兴程,杜娟.徐州市城郊雾霾天气中PM2.5物化及微生物特征分析.广东化工,2017,44(1):18- 18,38- 38.
[23] Dong L J,Qi J H,Shao C C,Zhong X,Gao D M,Cao W W,Gao J W,Bai R,Long G Y,Chu C C.Concentration and size distribution of total airborne microbes in hazy and foggy weather.Science of the Total Environment,2016,541:1011- 1018.
[24] Gao M,Jia R Z,Qiu T L,Han M L,Song Y,Wang X M.Seasonal size distribution of airborne culturable bacteria and fungi and preliminary estimation of their deposition in human lungs during non-haze and haze days.Atmospheric Environment,2015,118:203- 210.
[25] 高敏,仇天雷,贾瑞志,韩梅琳,宋渊,王旭明.北京雾霾天气生物气溶胶浓度和粒径特征.环境科学,2014,35(12):4415- 4421.
[26] 付红蕾.西安市不同天气可培养微生物气溶胶的分布特性研究[D].西安:长安大学,2016.
[27] 李婉欣,路瑞,谢铮胜,王金龙,范春兰,刘鹏霞,李彦鹏.西安市秋冬季不同空气质量下可培养微生物气溶胶浓度和粒径分布.环境科学,2017,38(11):4494- 4500.
[28] 路瑞,李婉欣,宋颖,谢铮胜,李彦鹏.西安市不同天气下可培养微生物气溶胶浓度变化特征.环境科学研究,2017,30(7):1012- 1019.
[29] 胡凌飞,张柯,王洪宝,李娜,王洁,杨文慧,殷喆,焦周光,温占波,李劲松.北京雾霾天大气颗粒物中微生物气溶胶的浓度及粒谱特征.环境科学,2015,36(9):3144- 3149.
[30] Gao M,Yan X,Qiu T L,Han M L,Wang X M.Variation of correlations between factors and culturable airborne bacteria and fungi.Atmospheric Environment,2016,128:10- 19.
[31] Gao M,Qiu T L,Jia R Z,Han M L,Song Y,Wang X M.Concentration and size distribution of viable bioaerosols during non-haze and haze days in Beijing.Environmental Science and Pollution Research,2015,22(6):4359- 4368.
[32] 韩晨,谢绵测,祁建华,张文倩,李先国,张大海.青岛市不同空气质量下可培养生物气溶胶分布特征及影响因素.环境科学研究,2016,29(9):1264- 1271.
[33] 薛林贵,姜金融,Famous E.城市空气微生物的监测及研究进展.环境工程,2017,35(3):152- 157,162- 162.
[34] 于玺华.现代空气微生物学.北京:人民军医出版社,2002.
[35] 方治国,欧阳志云,胡利锋,王效科,林学强.室外空气细菌群落特征研究进展.应用与环境生物学报,2005,11(1):123- 128.
[36] Shaffer B T,Lighthart B.Survey of culturable airborne bacteria at four diverse locations in Oregon:urban,rural,forest,and coastal.Microbial Ecology,1997,34(3):167- 177.
[37] Fang Z G,Ouyang Z Y,Zheng H,Wang X K.Concentration and size distribution of culturable airborne microorganisms in outdoor environments in Beijing,China.Aerosol Science and Technology,2008,42(5):325- 334.
[38] Li M F,Qi J H,Zhang H D,Huang S,Li L,Gao D M.Concentration and size distribution of bioaerosols in an outdoor environment in the Qingdao coastal region.Science of the Total Environment,2011,409(19):3812- 3819.
[39] Yan D,Zhang T,Su J,Zhao L L,Wang H,Fang X M,Zhang Y Q,Liu H Y,Yu L Y.Diversity and composition of airborne fungal community associated with particulate matters in beijing during haze and Non-haze days.Frontiers in Microbiology,2016,7:487.
[40] 姚文冲.基于高通量测序的南方典型旅游城市空气细菌群落特征研究[D].杭州:浙江工商大学,2017.
[41] 李林佳.青岛市不同季节雾霾期与非雾霾期空气微生物多样性比较研究[D].青岛:青岛理工大学,2015.
[42] 陆德源.医学微生物学(第五版).北京:人民卫生出版社,2001:69- 86.
[43] Kaushik R,Balasubramanian R.Assessment of bacterial pathogens in fresh rainwater and airborne particulate matter using Real-Time PCR.Atmospheric Environment,2012,46:131- 139.
[44] 周蓉,朱卫民.肺炎克雷伯菌分子流行病学及耐药机制研究进展.国外医药抗生素分册,2012,33(1):1- 5,42- 42.
[45] 任南,文细毛,吴安华.2014年全国医院感染横断面调查报告.中国感染控制杂志,2016,15(2):83- 87.
[46] U.S.Environmental Protection Agency.Product performance test guidelines:OCSPP 810.2500:air sanitizers—efficacy data recommendations.(2012).https://www.regulations.gov/contentStreamer?documentId=EPA-HQ-OPPT- 2009-0150-0025&contentType=pdf.
[47] Fang Z G,Ouyang Z Y,Zheng H,Wang X K,Hu L F.Culturable airborne bacteria in outdoor environments in Beijing,China.Microbial Ecology,2007,54(3):487- 496.
[48] Woo A C,Brar M S,Chan Y,Lau M C Y,Leung F C C,Scott J A,Vrijmoed L L P,Zawar-Reza P Z,Pointing S B.Temporal variation in airborne microbial populations and microbially-derived allergens in a tropical urban landscape.Atmospheric Environment,2013,74:291- 300.
[49] 陈佰义,何礼贤,胡必杰,倪语星,邱海波,石岩,施毅,王辉,王明贵,杨毅,张菁,俞云松.中国鲍曼不动杆菌感染诊治与防控专家共识.中华医学杂志,2012,92(2):76- 85.
[50] Fahlgren C,Hagstr?m ?,Nilsson D,Zweifel U L.Annual variations in the diversity,viability,and origin of airborne bacteria.Applied and Environmental Microbiology,2010,76(9):3015- 3025.
[51] 高秀娟,张晓丽,刘骙骙,张瑜.某医院临床分离阴沟肠杆菌分布及耐药性变迁.中国消毒学杂志,2017,34(4):350- 352.
[52] 姚雪华,杨跃杰,王文豪.破伤风患者预后的影响因素分析.中国实用医药,2016,11(17):90- 92.
[53] 蒋琪霞,王建东,徐元玲,彭青,周昕,李洋,黄秀玲.慢性伤口感染常见病原菌及其干预效果研究.护理学杂志,2015,30(12):19- 23.
[54] 王新华.住院患者1304例呼吸道感染原体检测结果分析.实用医技杂志,2015,22(2):167- 168.
[55] 刘丹,赵雨佳,王祎星,张慧峰,高慧明,周辉.雾霾和沙尘暴天气细颗粒物对人角质细胞促炎症作用和过敏反应的影响.毒理学杂志,2017,31(2):102- 108.
[56] 邱勇,张志红.大气细颗粒物免疫毒性研究进展.环境与健康杂志,2011,28(12):1117- 1120.
[57] Mancebo S E,Wang S Q.Recognizing the impact of ambient air pollution on skin health.Journal of the European Academy of Dermatology and Venereology,2015,29(12):2326- 2332.
[58] 吴迪,李潇,卢永波.雾霾对皮肤屏障功能的毒性作用研究//中国毒理学会,湖北省科学技术协会.中国毒理学会第七次全国毒理学大会暨第八届湖北科技论坛论文集.武汉:中国毒理学会,湖北省科学技术协会,2015:1.
[59] 肖纯凌,韩秀珍,魏德洲,孙文娟,叶丽杰,席淑华,崔金山.大气污染对人体免疫功能及微生态的影响.中国微生态学杂志,2002,14(1):12- 13.
[60] 肖纯凌.大气污染与呼吸道微生态.沈阳医学院学报,2006,8(1):1- 3.
[61] 张瑜,肖纯凌.呼吸道微生态在大气污染中的改变与调节的研究进展.中国当代医药,2017,24(12):12- 15.
[62] 仲崇山.雾霾诱发呼吸道感染者增多.新华日报,2010- 10- 12(A06).
[63] 李惠,赵学良,程芳,王辉,金小力.雾霾天气对荨麻疹就诊量和病情的影响.皮肤性病诊疗学杂志,2017,24(2):112- 115.
[64] Hussey S J K,Purves J,Allcock N,Fernandes V E,Monks P S,Ketley J M,Andrew P W,Morrissey J A.Air pollution alters Staphylococcus aureus and Streptococcus pneumoniae biofilms,antibiotic tolerance and colonisation.Environmental Microbiology,2017,19(5):1868- 1880.
[65] 高丽娜.冬季雾霾天气手术室空气净化效果观察.中国消毒学杂志,2016,33(12):1227- 1228.