入侵重庆地区的草地贪夜蛾种群生物型鉴定
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摘要
草地贪夜蛾是一种跨国迁飞性农业重大害虫,目前已在重庆的巫山、巫溪、云阳、北碚、潼南等地区出现并有迁飞的趋势.利用两个分子标记基因COI和Tpi对重庆地区采集的样本进行遗传特征比较,结果表明COI基因的亚型全为"水稻型",而Tpi基因的亚型既有"水稻型"又有"玉米型",说明入侵到重庆地区的草地贪夜蛾来源可能并不单一,暗示了重庆地区的草地贪夜蛾有可能存在分化为"水稻型"的风险.分析了入侵重庆地区的草地贪夜蛾相关的遗传特征,其结果不仅有助于认识草地贪夜蛾在中国大面积迁飞过程中发生的种群差异变化,也有利于农业部门根据重庆地区的地理环境属性,开展有针对性的监测防控工作.
Fall armyworm(FAW, Spodoptera frugiperda) is one of the most important agricultural pests, which has a great flight capacity. This insect pest is native to tropical and subtropical regions of the Americas, but recently it has been detected in Wushan, Wuxi, Yunyang, Beibei, Tongnan and other areas of Chongqing. It prefers corn, but can feed on more than 80 additional species of plants, including rice, sorghum and others. In an experiment reported herein, we compared the genetic characteristics of the pest samples collected from Chongqing by using two molecular marker genes(COI and Tpi). The results showed that all the samples belonged to Rice-strain when COI was used as the marker, and both Corn-strain and Rice-strain were present when Tpi was used as the marker, which suggested that the source of S. frugiperda might be variable, and that there is a potential risk of S. frugiperda differentiating into Rice-strain. The analysis of the genetic characteristics of S. frugiperda in Chongqing is helpful to working out corresponding prevention and control measures in combination with the geographical and environmental features in Chongqing, and sheds more light on the understanding of the population differences during the large-scale migration and flight of S. frugiperda in China.
Fall armyworm(FAW, Spodoptera frugiperda) is one of the most important agricultural pests, which has a great flight capacity. This insect pest is native to tropical and subtropical regions of the Americas, but recently it has been detected in Wushan, Wuxi, Yunyang, Beibei, Tongnan and other areas of Chongqing. It prefers corn, but can feed on more than 80 additional species of plants, including rice, sorghum and others. In an experiment reported herein, we compared the genetic characteristics of the pest samples collected from Chongqing by using two molecular marker genes(COI and Tpi). The results showed that all the samples belonged to Rice-strain when COI was used as the marker, and both Corn-strain and Rice-strain were present when Tpi was used as the marker, which suggested that the source of S. frugiperda might be variable, and that there is a potential risk of S. frugiperda differentiating into Rice-strain. The analysis of the genetic characteristics of S. frugiperda in Chongqing is helpful to working out corresponding prevention and control measures in combination with the geographical and environmental features in Chongqing, and sheds more light on the understanding of the population differences during the large-scale migration and flight of S. frugiperda in China.
引文
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[9] LEVY H C,GARCIA-MARUNIAK A,MARUNIAK J E.Strain Identification of Spodoptera frugiperda (lepidoptera:Noctuidae) Insects and Cell Line:Pcr-Rflp of Cytochrome Oxidase c Subunit i Gene [J].Florida Entomologist,2002,85(1):186-190.
[10] NAGOSHI R N,MEAGHER R L,FLANDERS K,et al.Using Haplotypes to Monitor the Migration of Fall Armyworm (Lepidoptera:Noctuidae) Corn-Strain Populations from Texas and Florida [J].Journal of Economic Entomology,2008,101(3):742-749.
[11] NAGOSHI R N,MEAGHER R L.Using Intron Sequence Comparisons in Thetriose-Phosphate Isomerase gene to Study the Divergence of the Fall Armyworm Host Strains [J].Insect Molecular Biology,2016,25(3):324-337.
[12] NAGOSHI R N.The Fall Armyworm Triose Phosphate Isomerase (Tpi) Gene as a Marker of Strain Identity and Interstrain Mating [J].Annals of the Entomological Society of America,2010,103(2):283-292.
[13] ARSENEAU J R,STEEVES R,LAFLAMME M.Modified Low-Salt CTAB Extraction of High-Quality DNA from Contaminant-Rich Tissues [J].Molecular Ecology Resources,2017,17(4):686-693.
[14] 张磊,靳明辉,张丹丹,等.入侵云南草地贪夜蛾的分子鉴定 [J].植物保护,2019,45(2):19-24,56.
[15] 张磊,柳贝,姜玉英,等.中国不同地区草地贪夜蛾种群生物型分子特征分析 [J/OL].植物保护:1-10 (2019-06-17)[2019-06-27].http://kns.cnki.net/KCM/detail/11.1982.S2019-0617.0915.001.html.
[16] 陈冬平,陈志廷,徐汉虹,等.不同分子标记对入侵广州草地贪夜蛾的寄主型鉴别能力比较 [J/OL].华南农业大学学报:1-5 (2019-06-17)[2019-06-27].http://kns.cnki.net/kcms/detail/44.1110.S.20190617.1027.002.html.
[17] GARCIA G,GODOY C,THOMAS G,et al.Delimiting Strategic Zones for the Development of Fall Armyworm (Lepidoptera:Noctuidae) on Corn in the State of Florida [J].Journal of Economic Entomology,2018,111(1):120-126.
[2] 郭井菲,静大鹏,太红坤,等.草地贪夜蛾形态特征及与3种玉米田为害特征和形态相近鳞翅目昆虫的比较 [J].植物保护,2019,45(2):7-12.
[3] DUMAS P,LEGEAI F,LEMAITRE C,et al.Spodoptera frugiperda (Lepidoptera:Noctuidae) Host-Plant Variants:Two Host Strains or Two Distinct Species?[J].Genetica,2015,143(3):305-316.
[4] UNBEHEND M,H?NNIGER S,MEAGHER R L,et al.Pheromonal Divergence between Two Strains of Spodoptera frugiperda [J].Journal of Chemical Ecology,2013,39(3):364-376.
[5] NAGOSHI R N,MEAGHER R L.Behavior and Distribution of the Two Fall Armyworm Host Strains in Florida [J].Florida Entomologist,2004,87(4):440-449.
[6] KOST S,HECKEL D G,YOSHIDO A,et al.A Z-Linked Sterility Locus Causes Sexual Abstinence in Hybrid Females and Facilitates Speciation in Spodoptera frugiperda [J].Evolution,2016,70(6):1418-1427.
[7] HAJIBABAEI M,JANZEN D H,BURNS J M,et al.DNA Barcodes Distinguish Species of Tropical Lepidoptera [J].Proceedings of the National Academy of Sciences,2006,103(4):968-971.
[8] NAGOSHI R N,MEAGHER R L,HAY-ROE M.Inferring the Annual Migration Patterns of Fall Armyworm (Lepidoptera:Noctuidae) in the United States from Mitochondrial Haplotypes [J].Ecology and Evolution,2012,2(7):1458-1467.
[9] LEVY H C,GARCIA-MARUNIAK A,MARUNIAK J E.Strain Identification of Spodoptera frugiperda (lepidoptera:Noctuidae) Insects and Cell Line:Pcr-Rflp of Cytochrome Oxidase c Subunit i Gene [J].Florida Entomologist,2002,85(1):186-190.
[10] NAGOSHI R N,MEAGHER R L,FLANDERS K,et al.Using Haplotypes to Monitor the Migration of Fall Armyworm (Lepidoptera:Noctuidae) Corn-Strain Populations from Texas and Florida [J].Journal of Economic Entomology,2008,101(3):742-749.
[11] NAGOSHI R N,MEAGHER R L.Using Intron Sequence Comparisons in Thetriose-Phosphate Isomerase gene to Study the Divergence of the Fall Armyworm Host Strains [J].Insect Molecular Biology,2016,25(3):324-337.
[12] NAGOSHI R N.The Fall Armyworm Triose Phosphate Isomerase (Tpi) Gene as a Marker of Strain Identity and Interstrain Mating [J].Annals of the Entomological Society of America,2010,103(2):283-292.
[13] ARSENEAU J R,STEEVES R,LAFLAMME M.Modified Low-Salt CTAB Extraction of High-Quality DNA from Contaminant-Rich Tissues [J].Molecular Ecology Resources,2017,17(4):686-693.
[14] 张磊,靳明辉,张丹丹,等.入侵云南草地贪夜蛾的分子鉴定 [J].植物保护,2019,45(2):19-24,56.
[15] 张磊,柳贝,姜玉英,等.中国不同地区草地贪夜蛾种群生物型分子特征分析 [J/OL].植物保护:1-10 (2019-06-17)[2019-06-27].http://kns.cnki.net/KCM/detail/11.1982.S2019-0617.0915.001.html.
[16] 陈冬平,陈志廷,徐汉虹,等.不同分子标记对入侵广州草地贪夜蛾的寄主型鉴别能力比较 [J/OL].华南农业大学学报:1-5 (2019-06-17)[2019-06-27].http://kns.cnki.net/kcms/detail/44.1110.S.20190617.1027.002.html.
[17] GARCIA G,GODOY C,THOMAS G,et al.Delimiting Strategic Zones for the Development of Fall Armyworm (Lepidoptera:Noctuidae) on Corn in the State of Florida [J].Journal of Economic Entomology,2018,111(1):120-126.