肉苁蓉多糖闪式提取工艺及其抗糖基化分析
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摘要
【目的】确定肉苁蓉多糖的闪式提取工艺条件并分析其抗糖基化活性.【方法】采用单因素和响应面试验优化料液比、转速以及提取时间对多糖得率的影响,及其对赖氨酸/葡萄糖模拟体系中形成的晚期糖基化终产物(AGEs)的抑制效果.【结果】当料液比1∶53 (g/mL)、提取转速5 387 r/min、提取时间60 s时,多糖得率可达12.35%.制备多糖和阳性对照氨基胍(AG)对模拟体系中AGEs半数抑制浓度IC_(50)分别为0.438和0.341 mg/mL.【结论】采用闪式提取肉苁蓉多糖方法简单可行,回收率高,其多糖具有一定糖基化抑制效果.
【Objective】 To optimize the extraction process of polysaccharides from Cistanche deserticola(CDPs) using flash extraction,and to analyze antiglycosylation activity.【Method】 The effect of different parameters ranging from solid-liquid ratio,voltage,and extraction time on the yield of CDPs were optimized through single factor experiments and response surface experiments.The effects of CDPs on the advanced glycation end products(AGEs) in Lysine/glucose models were also investigated.【Result】 The optimal extraction conditions with a yield of 12.35% for extraction of CDPs were the ratio of solid to liquid of 1∶53(g/mL),a rotating speed of 5387 r/min,and the extraction time for 1 min.The IC_(50) values of CDPs and AG against AGEs were 0.438 and 0.341 mg/mL,respectively.【Conclusion】 As a simple and efficient technique,it can be used to extract CDPs,which possess the inhibitory effects of antiglycosylation activity.
【Objective】 To optimize the extraction process of polysaccharides from Cistanche deserticola(CDPs) using flash extraction,and to analyze antiglycosylation activity.【Method】 The effect of different parameters ranging from solid-liquid ratio,voltage,and extraction time on the yield of CDPs were optimized through single factor experiments and response surface experiments.The effects of CDPs on the advanced glycation end products(AGEs) in Lysine/glucose models were also investigated.【Result】 The optimal extraction conditions with a yield of 12.35% for extraction of CDPs were the ratio of solid to liquid of 1∶53(g/mL),a rotating speed of 5387 r/min,and the extraction time for 1 min.The IC_(50) values of CDPs and AG against AGEs were 0.438 and 0.341 mg/mL,respectively.【Conclusion】 As a simple and efficient technique,it can be used to extract CDPs,which possess the inhibitory effects of antiglycosylation activity.
引文
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[16] 卫强,任定美,李四聪,等.皖南山区红豆杉多糖提取,纯化方法及单糖组成分析[J].食品科学,2017,38(16):190-197.
[17] 李明华,陆正清,孟秀梅,等.金针菇多糖闪式提取工艺及其抗氧化活性研究[J].食品与发酵工业,2016,42(10):216.
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[21] 欧阳杰,赵兵,王晓东,等.循环超声强化提取肉苁蓉中多糖和甜菜碱[J].过程工程学报,2003,3(3):227-230.
[22] 王全泽,袁堂丰,刘磊磊,等.响应面法优化闪式提取罗汉松总黄酮及其抗氧化活性[J].精细化工,2018,35(1):65-71.
[23] 刘贵梅,章鼎敏,李普,等.大豆 7S蛋白-糖体系晚期糖基化终产物形成因素[J].食品科学,2017,38(15):20-25.
[24] 张霞,张訸,但汉龙,等.玫瑰花蒂多酚的超声辅助提取工艺优化及其抗氧化活性评价[J].甘肃农业大学学报,2017,52(3):102-109.
[25] 张晓静,龚婷,扶庆权,等.响应面法优化微波辅助提取苹果皮中总黄酮的工艺研究[J].甘肃农业大学学报,2015,50(6):148-155.
[2] Macías-Cervantes M H,Rodríguez-Soto J M D,Uribarri J,et al.Effect of an advanced glycation end product-restricted diet and exercise on metabolic parameters in adult overweight men[J].Nutrition,2015,31(3):446-451.
[3] 马宗艳,蔡宏斌,葛朝明.晚期糖基化终末产物与阿尔茨海默病的关系[J].解放军医学杂志,2018,1:016.
[4] Xiong Q,Kadota S,TANI T,et al.Antioxidative effects of phenylethanoids from Cistanche deserticola[J].Biological and Pharmaceutical Bulletin,1996,19(12):1580-1585.
[5] Li W,Qiang C,Bo Y,et al.Screening of phytoestrogenic effective extracts and dose of Cistanche deserticola[J].Chinese Herbal Medicines,2013,5(4):292-296.
[6] Jia Y,Guan Q,Guo Y,et al.Echinacoside stimulates cell proliferation and prevents cell apoptosis in intestinal epithelial MODE-K cells by up-regulation of transforming growth factor-β1 expression[J].Journal of Pharmacological Sciences,2012,118(1):99-108.
[7] Lin L W,Hsieh M T,Tsai F H,et al.Anti-nociceptive and anti-inflammatory activity caused by Cistanche deserticola in rodents[J].Journal of Ethnopharmacology,2002,83(3):177-182.
[8] Li T M,Huang H C,Su C M,et al.Cistanche deserticola extract increases bone formation in osteoblasts[J].Journal of Pharmacy and Pharmacology,2012,64(6):897-907.
[9] Liang H,Yu F,Tong Z,et al.Effect of Cistanches Herba aqueous extract on bone loss in ovariectomized rat[J].International Journal of Molecular Sciences,2011,12(8):5060-5069.
[10] Cai R L,Yang M H,Shi Y,et al.Antifatigue activity of phenylethanoid-rich extract from Cistanche deserticola[J].Phytotherapy Research,2010,24(2):313-315.
[11] Geng X,Tian X,Tu P,et al.Neuroprotective effects of echinacoside in the mouse MPTP model of Parkinson's disease[J].European Journal of Pharmacology,2007,564(1-3):66-74.
[12] Xiong W T,Gu L,Wang C,et al.Anti-hyperglycemic and hypolipidemic effects of Cistanche tubulosa in type 2 diabetic db/db mice[J].Journal of Ethnopharmacology,2013,150(3):935-945.
[13] Dong Q,Yao J,Fang J,et al.Structural characterization and immunological activity of two cold-water extractable polysaccharides from Cistanche deserticola YC Ma[J].Carbohydrate research,2007,342(10):1343-1349.
[14] Fan Y,Yan C P,Chen C,et al.Tissue-smashing based ultra-rapid extraction of chemical constituents in herbal medicines[J].Journal of Pharmaceutical and Biomedical Analysis,2014,95:213-219.
[15] Tang W,Zhao Y.Evaluation of fatty acid profiles of four kinds of Osmanthus fragrans seeds by combined smash tissue extraction and GC/MS analysis[J].European Journal of Integrative Medicine,2014,6(6):724-724.
[16] 卫强,任定美,李四聪,等.皖南山区红豆杉多糖提取,纯化方法及单糖组成分析[J].食品科学,2017,38(16):190-197.
[17] 李明华,陆正清,孟秀梅,等.金针菇多糖闪式提取工艺及其抗氧化活性研究[J].食品与发酵工业,2016,42(10):216.
[18] 程振玉,宋海燕,杨英杰,等.不同提取方法对北五味子多糖提取率的影响[J].湖北农业科学,2015,54(1):159-163.
[19] 肖道安.肉苁蓉多糖的提取工艺优化[J].世界科学技术-中医药现代化,2014(4):926-929.
[20] 王晓琴,曹礼,朱艳萍.肉苁蓉多糖提取工艺及抑菌作用研究[J].安徽农业科学,2009,37(32):15855-15856.
[21] 欧阳杰,赵兵,王晓东,等.循环超声强化提取肉苁蓉中多糖和甜菜碱[J].过程工程学报,2003,3(3):227-230.
[22] 王全泽,袁堂丰,刘磊磊,等.响应面法优化闪式提取罗汉松总黄酮及其抗氧化活性[J].精细化工,2018,35(1):65-71.
[23] 刘贵梅,章鼎敏,李普,等.大豆 7S蛋白-糖体系晚期糖基化终产物形成因素[J].食品科学,2017,38(15):20-25.
[24] 张霞,张訸,但汉龙,等.玫瑰花蒂多酚的超声辅助提取工艺优化及其抗氧化活性评价[J].甘肃农业大学学报,2017,52(3):102-109.
[25] 张晓静,龚婷,扶庆权,等.响应面法优化微波辅助提取苹果皮中总黄酮的工艺研究[J].甘肃农业大学学报,2015,50(6):148-155.