野生胡麻愈伤组织诱导及其对NaCl胁迫的生理响应
|
摘要
对从国内征集的8份野生胡麻种质资源进行愈伤组织诱导,筛选最佳诱导培养基。对愈伤组织进行不同浓度NaCl胁迫,测定耐盐型和敏感型野生胡麻愈伤生理指标,评价其耐盐性,以探明其对NaCl胁迫的生理响应。结果表明,对野生胡麻愈伤诱导效果最好的培养基为MS+NAA(0.15 mg/L)+6-BA(1.5 mg/L),愈伤诱导率从大到小依次为乌兰察布蓝花、张家口蓝花、定西蓝花、平凉蓝花、会宁蓝花、兰州蓝花、临洮蓝花、乌兰察布红花。耐盐性由强到弱依次为乌兰察布蓝花、定西蓝花、会宁蓝花、兰州蓝花、张家口蓝花、平凉蓝花、临洮蓝花、乌兰察布红花。耐盐性野生胡麻愈伤组织在NaCl胁迫下SOD、CAT和POD等酶活性较敏感型种质更高,MDA质量分数变化幅度较敏感型种质小,表明耐盐性种质在盐胁迫下膜细胞受盐害程度相对较小,保护酶修复能力强,保护性作用能够持续稳定发挥。
In this study,8 materials of wild flax were used to induce callus and screen the perfect culture medium;the salt tolerance of these materials were evaluated by NaCl stress conducted on callus;the physiological response of the haloduric and sensitive wild flax were tested,to investigate its physiological response to NaCl stress. The results show that the optimal culture medium to callus induction of wild flax was MS+NAA(0.15 mg/L)+6-BA(1.5 mg/L). The callus induction rates ranged from high to low in order of Blue Flower in Wulanchabu,Blue Flower in Zhangjiakou,Blue Flower in Dingxi,Blue Flower in Pingliang,Blue Flower in Huining,Blue Flower in Lintao,and Red Flower in Wulanchabu. The salt tolerance ranged from strong to weak in order of Blue Flower in Wulanchabu,Blue Flower in Huining,Blue Flower in Pingliang,Blue Flower in Lintao,and Red Flower in Wulanchabu. The callus under NaCl stress of haloduric wild flax had higher activities of SOD,CAT and POD,and smaller variation in MDA mass fraction than those of sensitive wild flax,which indicated that the salt-tolerant germplasm had relatively small salt damage to the membrane cells under salt stress,strong protective enzyme repair ability and sustained and stable protective effect.
In this study,8 materials of wild flax were used to induce callus and screen the perfect culture medium;the salt tolerance of these materials were evaluated by NaCl stress conducted on callus;the physiological response of the haloduric and sensitive wild flax were tested,to investigate its physiological response to NaCl stress. The results show that the optimal culture medium to callus induction of wild flax was MS+NAA(0.15 mg/L)+6-BA(1.5 mg/L). The callus induction rates ranged from high to low in order of Blue Flower in Wulanchabu,Blue Flower in Zhangjiakou,Blue Flower in Dingxi,Blue Flower in Pingliang,Blue Flower in Huining,Blue Flower in Lintao,and Red Flower in Wulanchabu. The salt tolerance ranged from strong to weak in order of Blue Flower in Wulanchabu,Blue Flower in Huining,Blue Flower in Pingliang,Blue Flower in Lintao,and Red Flower in Wulanchabu. The callus under NaCl stress of haloduric wild flax had higher activities of SOD,CAT and POD,and smaller variation in MDA mass fraction than those of sensitive wild flax,which indicated that the salt-tolerant germplasm had relatively small salt damage to the membrane cells under salt stress,strong protective enzyme repair ability and sustained and stable protective effect.
引文
[1]赵玮,党占海,李闻娟.强抗旱胡麻新品种陇亚11号组织培养技术优化研究[J].中国沙漠,2012,32(5):1355-1361.
[2]党占海,赵蓉英,王敏,等.国际视野下胡麻研究的可视化分析[J].中国麻业科学,2010,32(6):305-307.
[3]王利民,张建平,米君,等.国外引进油用亚麻品种资源农艺性状分析[J].中国油料作物学报,2011,33(4):356-361.
[4]杜彦斌,王立军,张金,等.胡麻新品种天亚11号选育报告[J].甘肃农业科技,2018(2):24-26.
[5]赵利,党占海,李毅,等.亚麻籽的保健功能和开发利用[J].中国油脂,2006,31(3):71-73.
[6]赵玮,党占海,张建平,等.胡麻胚状体诱导及植株再生的研究[J].北方园艺,2010(21):174-176.
[7]颉瑞霞,张建平,党占海,等.激素和低温预处理对胡麻花药愈伤组织诱导的影响[J].分子植物育种,2009,7(6):1180-1185.
[8]孙上峰,王树彦.提高胡麻花药培养中愈伤组织诱导率的关键技术研究[J].内蒙古农业科技,2009(3):36-38.
[9]姬妍茹,田玉杰,苑志辉,等.用组织培养法繁殖野生亚麻的研究[J].中国麻业,2001,23(4):8-11.
[10]郭建荣,郑聪聪,李艳迪,等.Na Cl处理对真盐生植物盐地碱蓬根系特征及活力的影响[J].植物生理学报,2017,53(1):63-70.
[11]赵玮,党占海,张建平,等.Na Cl胁迫对不同抗旱强度胡麻品种农艺性状和生理指标的影响[J].甘肃农业科技,2016(11):1-6.
[12]高晶,那日,朝鲁蒙,等.电晕电场将大豆Gy3基因导入花棒愈伤织及植株再生[J].中国沙漠,2011,31(5):1202-1207.
[13]崔林,范银燕.裸燕麦胚性愈伤组织培养及悬浮系的建立[J].生物工程学报,1998,14(1):46-50.
[14]武冲,尹燕雷,陶吉寒,等.盐胁迫对低温预处理石榴种子萌发及幼苗生理生化的影响[J].西北植物学报,2015,35(1):161-167.
[15]李志萍,张文辉,崔豫川.Na Cl和Na2CO3胁迫对栓皮栎种子萌发及幼苗生长的影响[J].生态学报,2015,35(3):742-751.
[16]葛春晖,计巧灵,王雪华,等.亚麻耐盐性愈伤组织的生理生化特性[J].植物研究,2008,9(5):603-607.
[17]张兆英,于秀俊.植物抗盐性评价生理指标的分析[J].沧州师范专科学校学报,2006,22(4):50-55.
[18]BOSE J,RODRIGO-MORENO A,SHABALA S.ROShomeostasis in halophytes in the context of salinity stress tolerance[J].Journal of Experimental Botany,2014,65(5):1241-1257.
[19]张金林,李慧茹,郭姝媛,等.高等植物适应盐逆境研究进展[J].草业学报,2015,24(12):220-230.
[2]党占海,赵蓉英,王敏,等.国际视野下胡麻研究的可视化分析[J].中国麻业科学,2010,32(6):305-307.
[3]王利民,张建平,米君,等.国外引进油用亚麻品种资源农艺性状分析[J].中国油料作物学报,2011,33(4):356-361.
[4]杜彦斌,王立军,张金,等.胡麻新品种天亚11号选育报告[J].甘肃农业科技,2018(2):24-26.
[5]赵利,党占海,李毅,等.亚麻籽的保健功能和开发利用[J].中国油脂,2006,31(3):71-73.
[6]赵玮,党占海,张建平,等.胡麻胚状体诱导及植株再生的研究[J].北方园艺,2010(21):174-176.
[7]颉瑞霞,张建平,党占海,等.激素和低温预处理对胡麻花药愈伤组织诱导的影响[J].分子植物育种,2009,7(6):1180-1185.
[8]孙上峰,王树彦.提高胡麻花药培养中愈伤组织诱导率的关键技术研究[J].内蒙古农业科技,2009(3):36-38.
[9]姬妍茹,田玉杰,苑志辉,等.用组织培养法繁殖野生亚麻的研究[J].中国麻业,2001,23(4):8-11.
[10]郭建荣,郑聪聪,李艳迪,等.Na Cl处理对真盐生植物盐地碱蓬根系特征及活力的影响[J].植物生理学报,2017,53(1):63-70.
[11]赵玮,党占海,张建平,等.Na Cl胁迫对不同抗旱强度胡麻品种农艺性状和生理指标的影响[J].甘肃农业科技,2016(11):1-6.
[12]高晶,那日,朝鲁蒙,等.电晕电场将大豆Gy3基因导入花棒愈伤织及植株再生[J].中国沙漠,2011,31(5):1202-1207.
[13]崔林,范银燕.裸燕麦胚性愈伤组织培养及悬浮系的建立[J].生物工程学报,1998,14(1):46-50.
[14]武冲,尹燕雷,陶吉寒,等.盐胁迫对低温预处理石榴种子萌发及幼苗生理生化的影响[J].西北植物学报,2015,35(1):161-167.
[15]李志萍,张文辉,崔豫川.Na Cl和Na2CO3胁迫对栓皮栎种子萌发及幼苗生长的影响[J].生态学报,2015,35(3):742-751.
[16]葛春晖,计巧灵,王雪华,等.亚麻耐盐性愈伤组织的生理生化特性[J].植物研究,2008,9(5):603-607.
[17]张兆英,于秀俊.植物抗盐性评价生理指标的分析[J].沧州师范专科学校学报,2006,22(4):50-55.
[18]BOSE J,RODRIGO-MORENO A,SHABALA S.ROShomeostasis in halophytes in the context of salinity stress tolerance[J].Journal of Experimental Botany,2014,65(5):1241-1257.
[19]张金林,李慧茹,郭姝媛,等.高等植物适应盐逆境研究进展[J].草业学报,2015,24(12):220-230.