不同极性薏苡仁提取物抗氧化活性研究
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摘要
目的研究不同极性薏苡仁提取物的抗氧化活性,筛选出最佳抗氧化活性极性段。方法对薏苡仁粉末用95%乙醇进行回流提取,对乙醇提取物进行石油醚、氯仿、正丁醇、水萃取,得到石油醚部位、氯仿部位、正丁醇部位和水部位。通过测定各极性段的清除ABTS~+自由基、DPPH自由基和还原Fe~(3+)的能力,并与BHT进行比较,来研究不同极性部位的抗氧化活性。结果不同极性的薏苡仁提取物对DPPH自由基和ABTS~+自由基有不同程度的清除作用,并都具有一定的还原能力。其中四个提取部位对ABTS~+自由基清除能力大小为:水部位>正丁醇部位>氯仿部位>石油醚部位;对DPPH自由基清除能力大小为:正丁醇部位>水部位>氯仿部位>石油醚部位;对Fe~(3+)的还原能力能力大小顺序为:水部位>正丁醇部位>石油醚部位>氯仿部位,清除自由基能力和总还原能力均低于阳性对照物BHT。结论不同极性薏苡仁提取物具有一定的自由基清除作用和抗氧化作用。其中其中水部位有最好的清除ABTS~+自由基能力,正丁醇部位有最好的清除DPPH自由基能力,而水部位有最好的还原Fe~(3+)的能力。
Objective To study the antioxidant activity of different polar extracts of coicis, and screen for optimum antioxidant active polarity segment. Methods Coix seed was extracted using reflux method by 95% ethanol. Then the concentrated ethanol extract was partitioned between petroleum ether, chloroform, n-butanol and water, respectively, which gave the oil ether part, chloroform part, n-butanol part and water part. The antioxidant activity of different polar regions was studied by determining the scavenging ability of ABTS~+ free radicals and DPPH free radicals and the ability of restoring Fe~(3+), and compared with BHT. Results Different polar extracts had different degrees of clearance ability of DPPH free radicals and ABTS~+ free radicals, and had certain reduction capabilities. The order of scavenging capacity of four extracts to ABTS~+ free radicals was: water part > n-butanol part > chloroform part > petroleum ether part. The order of scavenging capacity of four extraction sites to DPPH radical was: n-butanol part > water part > chloroform part >petroleum ether part. The order of the capacity of the four extraction sites to the reduction ability of Fe~(3+) was:water part > n-butanol part > petroleum ether part >chloroform part. The scavenging free radical ability and total reduction ability were lower than the positive control BHT. Conclusion The different polar extracts of Coix seed have free radical scavenging ability and antioxidant effects. The water part has the best ability to scavenge ABTS~+ free radical, the n-butanol fraction has the best ability to scavenge DPPH free radical, and the water site has the best ability to restore Fe~(3+).
Objective To study the antioxidant activity of different polar extracts of coicis, and screen for optimum antioxidant active polarity segment. Methods Coix seed was extracted using reflux method by 95% ethanol. Then the concentrated ethanol extract was partitioned between petroleum ether, chloroform, n-butanol and water, respectively, which gave the oil ether part, chloroform part, n-butanol part and water part. The antioxidant activity of different polar regions was studied by determining the scavenging ability of ABTS~+ free radicals and DPPH free radicals and the ability of restoring Fe~(3+), and compared with BHT. Results Different polar extracts had different degrees of clearance ability of DPPH free radicals and ABTS~+ free radicals, and had certain reduction capabilities. The order of scavenging capacity of four extracts to ABTS~+ free radicals was: water part > n-butanol part > chloroform part > petroleum ether part. The order of scavenging capacity of four extraction sites to DPPH radical was: n-butanol part > water part > chloroform part >petroleum ether part. The order of the capacity of the four extraction sites to the reduction ability of Fe~(3+) was:water part > n-butanol part > petroleum ether part >chloroform part. The scavenging free radical ability and total reduction ability were lower than the positive control BHT. Conclusion The different polar extracts of Coix seed have free radical scavenging ability and antioxidant effects. The water part has the best ability to scavenge ABTS~+ free radical, the n-butanol fraction has the best ability to scavenge DPPH free radical, and the water site has the best ability to restore Fe~(3+).
引文
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[10]王彦芳,季旭明,赵海军,等.薏苡仁多糖不同组分对脾虚水湿不化大鼠模型免疫功能的影响[J].中华中医药杂志,2017,32(3):1303-1306.
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[13]Lee MY,Lin HY,Cheng F,et al.Isolation and characterization of new lactam compounds that inhibit lung and colon cancer cells from adlay(Coix lachryma-jobi L.var.ma-yuen Stapf)bran[J].Food Chem Toxicol,2008,46(6):1933-1939.
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[15]Huang DW,Chengpei C,Yuehhsiung K,et al.Identification of compounds in adlay(Coix lachryma-jobi L.var.ma-yuen Stapf)seed hull extracts that inhibit lipopolysaccharide-induced inflammation in RAW 264.7macrophages[J].J Agric Food Chem,2009,57(22):10651-10 657.
[16]Chen HJ,Chung CP,Chiang W,et al.Anti-inflammatory effects and chemical study of a flavonoid-enriched fraction from adiay bran[J].Food Chem,2011,126(4):1741-1748.
[17]黄娇,范世明,陈丹,等.闽产薏苡种皮与薏苡仁的化学成分分析[J].福建中医药,2016,47(5):18-20.
[18]马小庭,黄锁义.薏苡仁抗氧化活性研究进展[J].食品研究与开发,2017,38(7):188-191.
[19]赵婕,王明力,汤翠,等.薏苡仁功能活性成分的研究进展[J].食品工业科技,2016,37(18):374-377.
[20]陈雯静,黄锁义,喻巧容,等.不同极性薏苡茎提取物的抗氧化活性研究[J].食品工业,2017,38(6):104-106.
[21]张声源,庄远杯,廖梅,等.虎舌红不同溶剂提取物抗氧化活性研究[J].现代中药研究与实践,2017,31(2):39-42.
[22]张强,陆玲,李东,等.薏苡仁不同溶剂提取物体外抗氧化活性研究[J].食品工业,2017,38(9):152-154.
[2]Apirattananusorn S,Tongta S,Cui SW,et al.Chemical,molecular,and structural characterization of alkali extractable nonstarch polysaccharides from Job′s tear[J].J Agric Food Chem,2008,56(18):8549-8557.
[3]李斌,李玲玲,冀会方,等.薏苡仁谷蛋白源降压肽稳定性研究[J].中医学报,2015,43(6):53-57.
[4]李涂蓝,林明霞,黄锁义,等.薏苡茎总碱抗氧化性研究[J].中国野生植物资源,2013,32(6):16-18.
[5]谢燕飞,覃冬,潘廷啟,等.广西壮药薏苡茎多糖的提取及抗氧化性研究[J].中国处方药,2012,10(2):43-45.
[6]朱英,杨志峰.红腺忍冬叶不同溶剂提取物中咖啡酸和多糖的含量变化研究[J].浙江中医药大学学报,2015,39(11):824-828.
[7]耿春霞.浅谈薏苡仁在肿瘤治疗中的作用[J].云南中医中药杂志,2014,35(12):74-76.
[8]侯兰梅,王晓洁,张恩宁,等.麦薏粉对荷瘤小鼠晚期的辅助作用及其机制[J].鲁东大学学报,2016,32(3):229-235.
[9]林瑶,陆世惠,喻巧容,等.薏苡茎叶提取物石油醚部位的体外抗肿瘤活性研究[J].中国临床药理学杂志,2018,34(3):282-284.
[10]王彦芳,季旭明,赵海军,等.薏苡仁多糖不同组分对脾虚水湿不化大鼠模型免疫功能的影响[J].中华中医药杂志,2017,32(3):1303-1306.
[11]覃丽,蓝琳云,张强,等.薏苡茎多酚的体外抗氧化活性研究[J].食品工业,2017,38(12):177-179.
[12]樊青玲,张平,任旻琼.薏苡的化学成分、药理活性及应用研究进展[J].天然产物研究与开发,2015,27(10):1831-1835.
[13]Lee MY,Lin HY,Cheng F,et al.Isolation and characterization of new lactam compounds that inhibit lung and colon cancer cells from adlay(Coix lachryma-jobi L.var.ma-yuen Stapf)bran[J].Food Chem Toxicol,2008,46(6):1933-1939.
[14]Chung CP,Hsu CY,Lin JH,et al.Antiproliferative lactams and spiroenone from adlay bran in human breast cancer cell lines[J].J Agric Food Chem,2011,59(4):1185-1194.
[15]Huang DW,Chengpei C,Yuehhsiung K,et al.Identification of compounds in adlay(Coix lachryma-jobi L.var.ma-yuen Stapf)seed hull extracts that inhibit lipopolysaccharide-induced inflammation in RAW 264.7macrophages[J].J Agric Food Chem,2009,57(22):10651-10 657.
[16]Chen HJ,Chung CP,Chiang W,et al.Anti-inflammatory effects and chemical study of a flavonoid-enriched fraction from adiay bran[J].Food Chem,2011,126(4):1741-1748.
[17]黄娇,范世明,陈丹,等.闽产薏苡种皮与薏苡仁的化学成分分析[J].福建中医药,2016,47(5):18-20.
[18]马小庭,黄锁义.薏苡仁抗氧化活性研究进展[J].食品研究与开发,2017,38(7):188-191.
[19]赵婕,王明力,汤翠,等.薏苡仁功能活性成分的研究进展[J].食品工业科技,2016,37(18):374-377.
[20]陈雯静,黄锁义,喻巧容,等.不同极性薏苡茎提取物的抗氧化活性研究[J].食品工业,2017,38(6):104-106.
[21]张声源,庄远杯,廖梅,等.虎舌红不同溶剂提取物抗氧化活性研究[J].现代中药研究与实践,2017,31(2):39-42.
[22]张强,陆玲,李东,等.薏苡仁不同溶剂提取物体外抗氧化活性研究[J].食品工业,2017,38(9):152-154.