蛋白酶抑制子在植物与病原物互作中的作用
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摘要
蛋白酶抑制子(proteinase inhibitors,PIs)是普遍存在于生物体内的一类多肽和蛋白,通过位阻作用抑制蛋白酶的活性,在动物的生长发育及昆虫取食等方面具有重要作用.近些年的研究表明,PIs在植物抗病性中发挥着重要的作用.介绍了PIs的类别,综述了植物与病原物互作过程中植物PIs在增强或减弱抗病性与病原微生物在增强致病性方面的作用.
Proteinase inhibitors(PIs), were general polypeptide and protein in biology, played important roles in production and development of animal and insect herbivory by steric interaction. In recent year, studies suggested that PIs also played major roles in the interaction between plants and pathogens. In addition to introducing characteristic and the classification of PIs, it was reviewed the functions both of PIs from plants in enhancing/decreasing disease resistance and of PIs from pathogen in increasing susceptibility.
Proteinase inhibitors(PIs), were general polypeptide and protein in biology, played important roles in production and development of animal and insect herbivory by steric interaction. In recent year, studies suggested that PIs also played major roles in the interaction between plants and pathogens. In addition to introducing characteristic and the classification of PIs, it was reviewed the functions both of PIs from plants in enhancing/decreasing disease resistance and of PIs from pathogen in increasing susceptibility.
引文
[1]MANGAN M S J,KAISERMAN D,BIRD P I.The role of serpins in vertebrate immunity[J].Tissue Antigens,2008,72(1):1-10.
[2]李茶姿,苗爽,杨玲,等.植物蛋白酶抑制子的抗病性研究[J].浙江农业科学,2013(8):995-998.
[3]VAN D L K,HEMETSBERGER C,KASTNER C,et al.A maize cystatin suppresses host immunity by inhibiting apoplastic cysteine proteases[J].Plant Cell,2012,24(3):1285-1300.
[4]EDOUARD B F,SACHIN R,STEFFEN R,et al.A Kunitz-type protease inhibitor regulates programmed cell death during flower development in Arabidopsis thaliana[J].J Exp Bot,2015,66(20):6119-6135.
[5]王荣春,孙建华,何述栋,等.胰蛋白酶抑制剂的结构与功能研究进展[J].食品科学,2013,34(9):364-368.
[6]GAHLOTH D,SELVAKUMAR P,SHEE C,et al.Cloning,sequence analysis and crystal structure determination of a miraculin-like protein from Murraya koenigii[J].Arch Biochem Biophys,2010,494(1):15-22.
[7]曾仲奎,谢文胜,鲍锦库,等.水稻巯基蛋白酶抑制剂的纯化及其性质研究[J].中国生物化学与分子生物学报,1996,12(6):703-708.
[8]MAJOR I T,CONSTABEL C P.Functional analysis of the Kunitz trypsin inhibitor family in poplar reveals biochemical diversity and multiplicity in defense against herbivores[J].Plant Physiol,2008,146(3):888-903.
[9]BODE W,HUBER R.Natural protein proteinase inhibitors and their interaction with proteinases[J].Febs J,1992,204(2):433-451.
[10]HOPE-ONYEKWERE N S,OGUELI G I,CORTELAZZO A,et al.Effects of mucuna pruriens protease inhibitors on echis carinatus venom[J].Photosynth Res,2012,26(12):1913-1919.
[11]MELLO G C,OLIVA M L,SUMIKAWA J T,et al.Purification and characterization of a new trypsin inhibitor from Dimorphandra mollis seeds[J].J Pro Chem,2001,20(8):625-632.
[12]LASKOWSKI J M,KATO I.Protein inhibitors of proteinases[J].Annu Rev Biochem,1980,49(5):593-626.
[13]IRVING J A,PIKE R N,LESK A M,et al.Phylogeny of the serpin superfamily:Implications of patterns of amino acid conservation for structure and function[J].Genome Res,2000,10(12):1845-1864.
[14]RAWLINGS N D,TOLLE D P,Barrett A J.Evolutionary families of peptidase inhibitors[J].Bio Chem J,2004,378(3):705-716.
[15]WANG X,XUE Q,MAO X,et al.Two I84 family protease inhibitors from Chinese razor clams Sinonovacula constricta expressed in response to environmental challenges[J].Fish Shellfish Immun,2018,75:149-157.
[16]FAN S G,WU G J.Characteristics of plant proteinase inhibitors and their applications in combating phytophagous insects[J].Bot Bull Acad Sinica,2005,46(4):273-292.
[17]GUTIERREZCAMPOS R,TORRESACOSTA J A,SAUCEDOARIAS L J,et al.The use of cysteine proteinase inhibitors to engineer resistance against potyviruses in transgenic tobacco plants[J].Nat Bio Technol,1999,17(12):1223-1226.
[18]黄婷,袁治礼,成巨龙,等.烟草对蚀纹病毒和马铃薯Y病毒的抗病性鉴定[J].西北农林科技大学学报(自然科学版),2012,40(6):107-113.
[19]梁炫强,潘瑞炽,周桂元.花生种子胰蛋白酶抑制剂与抗黄曲霉侵染的关系[J].作物学报,2003,29(2):295-299.
[20]SUN D,CHEN J,ZHOU Z S,et al.Ectopic expression of a proteinase inhibitor I4(MtPiI4) gene from medicago truncatula confers plant resistance to Pseudomonas syringae pv.tomato DC3000[J].Plant Mol Biol Rep,2015,33(6):1686-1696.
[21]LAURA FELDMAN M,BALBINA ANDREU A,KORGAN S,et al.PLPKI:A novel serine protease inhibitor as a potential biochemical marker involved in horizontal resistance to Phytophthora infestans[J].Plant Breeding,2014,133(2):275-280.
[22]COSTA D C,VERMELHO A B,ALMEIDA C A,et al.Inhibitory effect of linalool-rich essential oil from Lippia alba on the peptidase and keratinase activities of dermatophytes[J].J Enzym Inhib,2014,29(1):12-17.
[23]LACERDA J T J G D,LACERDA R R E,ASSUNCAO N A,et al.New insights into lectin from Abelmoschus esculentus seeds as a Kunitz-type inhibitor and its toxic effects on Ceratitis capitata and root-knot nematodes Meloidogyne spp[J].Process Biochem,2017,63:96-104.
[24]XU Q F,CHENG W S,LI S S,et al.Identification of genes required for Cf-dependent hypersensitive cell death by combined proteomic and RNA interfering analyses[J].J Exp Bot,2012,63(7):2421-2435.
[25]MüNTZ K.Protein dynamics and proteolysis in plant vacuoles[J].J Exp Bot,2007,58(10):2391-2407.
[26]GRUDKOWSKA M,ZAGDA.Multifunctional role of plant cysteine proteinases[J].Acta Bio chim Pol,2004,51(3):609-624.
[27]罗岸.烟草早期胚胎发生中印迹基因的筛选与分析[D].武汉:武汉大学,2013.
[28]KIM N H,HWANG B K.Pepper pathogenesis-related protein 4c is a plasma-membrane localized cysteine protease inhibitor that is required for plant cell death and defense signaling[J].Plant J,2015,81(1):81-94.
[29]COLL N S,VERCAMMEN D,SMIDLER A,et al.Arabidopsis type Ⅰ metacaspases control cell death[J].Sci,2010,330(6009):1393-1397.
[30]LEMA ASQUI S,VERCAMMEN D,SERRANO I,et al.AtSERPIN1 is an inhibitor of the metacaspase AtMC1-mediated cell death and autocatalytic processing in planta[J].New Phytol,2018,218(3):1156-1166.
[31]TIAN M,CHAUDHRY F,RUZICKA D R,et al.Arabidopsis actin-depolymerizing factor AtADF4 Mediates defense signal transduction triggered by the pseudotnonas syringae effector AvrPphB[J].Plant Physiol,2009,150(2):815-824.
[32]RA V D H.Plant proteases:From phenotypes to molecular mechanisms[J].Annual Review of Plant Biol,2008,59(59):191-223.
[33]SONG J,WIN J,TIAN M,et al.Apoplastic effectors secreted by two unrelated eukaryotic plant pathogens target the tomato defense protease Rcr3[J].Proc Natl Acad Sci USA,2009,106(5):1654-1659.
[34]MUELLER A N,ZIEMANN S,TREITSCHKE S,et al.Compatibility in the Ustilago maydis-Maize interaction requires inhibition of host cysteine proteases by the fungal effector Pit2[J].Plos Pathog,2013,9(2):e1003177.
[35]SILVA Y,PORTIELES R,PUJOL M,et al.Expression of a microbial serine proteinase?Inhibitor gene enhances the tobacco defense against oomycete pathogens[J].Physiol Mol Plant Pathol,2013,84(5):99-106.
[2]李茶姿,苗爽,杨玲,等.植物蛋白酶抑制子的抗病性研究[J].浙江农业科学,2013(8):995-998.
[3]VAN D L K,HEMETSBERGER C,KASTNER C,et al.A maize cystatin suppresses host immunity by inhibiting apoplastic cysteine proteases[J].Plant Cell,2012,24(3):1285-1300.
[4]EDOUARD B F,SACHIN R,STEFFEN R,et al.A Kunitz-type protease inhibitor regulates programmed cell death during flower development in Arabidopsis thaliana[J].J Exp Bot,2015,66(20):6119-6135.
[5]王荣春,孙建华,何述栋,等.胰蛋白酶抑制剂的结构与功能研究进展[J].食品科学,2013,34(9):364-368.
[6]GAHLOTH D,SELVAKUMAR P,SHEE C,et al.Cloning,sequence analysis and crystal structure determination of a miraculin-like protein from Murraya koenigii[J].Arch Biochem Biophys,2010,494(1):15-22.
[7]曾仲奎,谢文胜,鲍锦库,等.水稻巯基蛋白酶抑制剂的纯化及其性质研究[J].中国生物化学与分子生物学报,1996,12(6):703-708.
[8]MAJOR I T,CONSTABEL C P.Functional analysis of the Kunitz trypsin inhibitor family in poplar reveals biochemical diversity and multiplicity in defense against herbivores[J].Plant Physiol,2008,146(3):888-903.
[9]BODE W,HUBER R.Natural protein proteinase inhibitors and their interaction with proteinases[J].Febs J,1992,204(2):433-451.
[10]HOPE-ONYEKWERE N S,OGUELI G I,CORTELAZZO A,et al.Effects of mucuna pruriens protease inhibitors on echis carinatus venom[J].Photosynth Res,2012,26(12):1913-1919.
[11]MELLO G C,OLIVA M L,SUMIKAWA J T,et al.Purification and characterization of a new trypsin inhibitor from Dimorphandra mollis seeds[J].J Pro Chem,2001,20(8):625-632.
[12]LASKOWSKI J M,KATO I.Protein inhibitors of proteinases[J].Annu Rev Biochem,1980,49(5):593-626.
[13]IRVING J A,PIKE R N,LESK A M,et al.Phylogeny of the serpin superfamily:Implications of patterns of amino acid conservation for structure and function[J].Genome Res,2000,10(12):1845-1864.
[14]RAWLINGS N D,TOLLE D P,Barrett A J.Evolutionary families of peptidase inhibitors[J].Bio Chem J,2004,378(3):705-716.
[15]WANG X,XUE Q,MAO X,et al.Two I84 family protease inhibitors from Chinese razor clams Sinonovacula constricta expressed in response to environmental challenges[J].Fish Shellfish Immun,2018,75:149-157.
[16]FAN S G,WU G J.Characteristics of plant proteinase inhibitors and their applications in combating phytophagous insects[J].Bot Bull Acad Sinica,2005,46(4):273-292.
[17]GUTIERREZCAMPOS R,TORRESACOSTA J A,SAUCEDOARIAS L J,et al.The use of cysteine proteinase inhibitors to engineer resistance against potyviruses in transgenic tobacco plants[J].Nat Bio Technol,1999,17(12):1223-1226.
[18]黄婷,袁治礼,成巨龙,等.烟草对蚀纹病毒和马铃薯Y病毒的抗病性鉴定[J].西北农林科技大学学报(自然科学版),2012,40(6):107-113.
[19]梁炫强,潘瑞炽,周桂元.花生种子胰蛋白酶抑制剂与抗黄曲霉侵染的关系[J].作物学报,2003,29(2):295-299.
[20]SUN D,CHEN J,ZHOU Z S,et al.Ectopic expression of a proteinase inhibitor I4(MtPiI4) gene from medicago truncatula confers plant resistance to Pseudomonas syringae pv.tomato DC3000[J].Plant Mol Biol Rep,2015,33(6):1686-1696.
[21]LAURA FELDMAN M,BALBINA ANDREU A,KORGAN S,et al.PLPKI:A novel serine protease inhibitor as a potential biochemical marker involved in horizontal resistance to Phytophthora infestans[J].Plant Breeding,2014,133(2):275-280.
[22]COSTA D C,VERMELHO A B,ALMEIDA C A,et al.Inhibitory effect of linalool-rich essential oil from Lippia alba on the peptidase and keratinase activities of dermatophytes[J].J Enzym Inhib,2014,29(1):12-17.
[23]LACERDA J T J G D,LACERDA R R E,ASSUNCAO N A,et al.New insights into lectin from Abelmoschus esculentus seeds as a Kunitz-type inhibitor and its toxic effects on Ceratitis capitata and root-knot nematodes Meloidogyne spp[J].Process Biochem,2017,63:96-104.
[24]XU Q F,CHENG W S,LI S S,et al.Identification of genes required for Cf-dependent hypersensitive cell death by combined proteomic and RNA interfering analyses[J].J Exp Bot,2012,63(7):2421-2435.
[25]MüNTZ K.Protein dynamics and proteolysis in plant vacuoles[J].J Exp Bot,2007,58(10):2391-2407.
[26]GRUDKOWSKA M,ZAGDA.Multifunctional role of plant cysteine proteinases[J].Acta Bio chim Pol,2004,51(3):609-624.
[27]罗岸.烟草早期胚胎发生中印迹基因的筛选与分析[D].武汉:武汉大学,2013.
[28]KIM N H,HWANG B K.Pepper pathogenesis-related protein 4c is a plasma-membrane localized cysteine protease inhibitor that is required for plant cell death and defense signaling[J].Plant J,2015,81(1):81-94.
[29]COLL N S,VERCAMMEN D,SMIDLER A,et al.Arabidopsis type Ⅰ metacaspases control cell death[J].Sci,2010,330(6009):1393-1397.
[30]LEMA ASQUI S,VERCAMMEN D,SERRANO I,et al.AtSERPIN1 is an inhibitor of the metacaspase AtMC1-mediated cell death and autocatalytic processing in planta[J].New Phytol,2018,218(3):1156-1166.
[31]TIAN M,CHAUDHRY F,RUZICKA D R,et al.Arabidopsis actin-depolymerizing factor AtADF4 Mediates defense signal transduction triggered by the pseudotnonas syringae effector AvrPphB[J].Plant Physiol,2009,150(2):815-824.
[32]RA V D H.Plant proteases:From phenotypes to molecular mechanisms[J].Annual Review of Plant Biol,2008,59(59):191-223.
[33]SONG J,WIN J,TIAN M,et al.Apoplastic effectors secreted by two unrelated eukaryotic plant pathogens target the tomato defense protease Rcr3[J].Proc Natl Acad Sci USA,2009,106(5):1654-1659.
[34]MUELLER A N,ZIEMANN S,TREITSCHKE S,et al.Compatibility in the Ustilago maydis-Maize interaction requires inhibition of host cysteine proteases by the fungal effector Pit2[J].Plos Pathog,2013,9(2):e1003177.
[35]SILVA Y,PORTIELES R,PUJOL M,et al.Expression of a microbial serine proteinase?Inhibitor gene enhances the tobacco defense against oomycete pathogens[J].Physiol Mol Plant Pathol,2013,84(5):99-106.