膝骨性关节炎动物模型选择与制备的比较
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摘要
膝骨性关节炎(KOA)是退行性、多发性、难以治愈的疾病。KOA动物疾病模型是研究KOA发病机制、治疗方法及起效机制的有效载体。目前,诱导KOA动物模型的方法多种多样,各有其特点和适用性,本文通过对KOA动物模型选择与制备方法的文献进行综述比较,结合笔者对KOA动物模型的研究实践,探讨实验性KOA动物模型的选择与制备,以期对研究KOA动物模型提供有效参考。
Knee osteoarthritis(KOA) is a degenerative and intractable disease with high morbidity. Animal models of KOA provide an effective tool for studying the disease pathogenesis, potential treatments and their mechanism of action. A variety of animal models currently exist for studies of KOA, each with its own characteristics and advantages. In addition to the author's own research on animal models of this disease, this article reviews the literature on method ologies for establishing animal models of KOA. This article explores the selection criteria and method of preparing experimental KOA animal models, in order to provide an effective reference for researchers who wish to apply these models to their own work.
Knee osteoarthritis(KOA) is a degenerative and intractable disease with high morbidity. Animal models of KOA provide an effective tool for studying the disease pathogenesis, potential treatments and their mechanism of action. A variety of animal models currently exist for studies of KOA, each with its own characteristics and advantages. In addition to the author's own research on animal models of this disease, this article reviews the literature on method ologies for establishing animal models of KOA. This article explores the selection criteria and method of preparing experimental KOA animal models, in order to provide an effective reference for researchers who wish to apply these models to their own work.
引文
[1] 黄桂成,王拥军.中医骨伤科学 [M].北京:中国中医药出版社,2016:321-322.
[2] 郭义.实验针灸学 [M].北京:中国中医药出版社,2016:19.
[3] 蒋洋洋,朱浩,张海.人类与小鼠同源lncRNA基因及其表观遗传调控靶基因分析 [J].南方医科大学学报,2018,38(6):731-735.
[4] 曾俊华,马笃军,彭力平,等.实验兔膝骨关节炎模型的建立及鉴定 [J].中国临床研究,2016,29(5):679-682.
[5] 尚平,贺宪,陈孝银,等.过伸位和过屈位固定复制骨性关节炎动物模型的比较 [J].生物骨科材料与临床研究,2006,3(1):11-14.
[6] 陈达,彭力平,廖州伟,等.木瓜蛋白酶与石膏制动建立兔膝骨关节炎模型的比较 [J].广东医学,2017,38(14):2114-2118.
[7] Hermeto LC,Derossi R,Oliveira RJ,et al.Effects of intra-articular injection of mesenchymal stem cells associated with platelet-rich plasma in a rabbit model of osteoarthritis [J].Genet Mol Res,2016,15(3).
[8] Hulth A,Lindberg L,Telhag H.Experimental osteoarthritis in rabbits.Preliminary report [J].Acta Orthop Scand,1970,41(5):522-530.
[9] 熊元,赵振国,李传郡,等.兔膝骨关节炎模型的制备及鉴定 [J].实验动物科学,2013,30(3):31-34,63.
[10] Roman-Blas JA,Mediero A,Tardío L,et al.The combined therapy with chondroitin sulfate plus glucosamine sulfate or chondroitin sulfate plus glucosamine hydrochloride does not improve joint damage in an experimental model of knee osteoarthritis in rabbits [J].Eur J Pharmacol,2017,794:8-14.
[11] 李炳辉,牛东生,孙玺淳,等.直立负重法与Hulth法建立兔膝骨关节炎模型的对比研究 [J].宁夏医科大学学报,2013,35(3):256-258,262.
[12] 黄吉利,罗明,潘捷,等.持续被动运动对兔骨关节炎软骨细胞凋亡相关基因P53、caspase-3、Bcl-2和Bax的影响 [J].中国老年学杂志,2016,36(24):6067-6069.
[13] 张冲,王恒树,阚秀莲,等.传统中药补肾固筋方对膝骨性关节炎IL-1、TNF-α的作用机制 [J].中国实验动物学报,2015,23(3):312-315.
[14] 钱洁,邢雪松,梁军.大鼠膝关节骨性关节炎动物模型的两种实验方案 [J].实验室研究与探索,2014,33(11):23-27.
[15] Kellyy S,Dunham JP,Murray F,et al.Spontaneous firing in C-fibers and increased mechanical sensitivity in A-fibers of knee joint-associated mechanoreceptive primary afferent neurones during MIA-induced osteoarthritis in the rat [J].Osteoarthr Cartil,2012,20(4):305-313.
[16] 汪宗保,徐亚林,廖威明,等.木瓜蛋白酶诱导大鼠膝早期骨关节炎软骨表面的电镜扫描 [J].中国组织工程研究,2014,18(2):177-182.
[17] 黄永明,潘建科,郭达,等.Ⅱ型胶原蛋白酶诱导SD大鼠膝骨关节炎模型的建立 [J].广东医学,2015,36(8):1145-1148.
[18] Salazar-Noratto GE,De Nijs N,Stevens HY,et al.Regional gene expression analysis of multiple tissues in an experimental animal model of post-traumatic osteoarthritis [J].Osteoarthr Cartil,2019,27(2):294-303.
[19] 李安琪,冯伟,何东仪,等.改良韧带缺损法建立大鼠膝骨关节炎模型 [J].上海中医药杂志,2018,52(5):13-17.
[20] Pernille HA,László BT,Thomas LA,et al.Ovariectomized rats as a model of postmenopausal osteoarthritis:validation and application [J].Arthritis Res Ther,2004,6(2):169-180.
[21] 谢靖,杨冠.一种新的半月板切断导致小鼠骨关节炎方法的建立 [J].军事医学,2013,37(9):708-711.
[22] van der Kraan PM,Vitters EL,van de Putte LB,et al.Development of osteoarthritic lesions in mice by “metabolic” and “mechanical” alterations in the knee joints [J].Am J Pathol,1989,135(6):1001-1014.
[23] Arita M,Li SW,Kopen G,et al.Skeletal abnormalities and ultrastructural changes of cartilage in transgenic mice expressing a collagen II gene (COL2A1) with a Cys for Arg-α1-519 substitution [J].Osteoarthr Cartil,2002,10(10):808-815.
[24] 曹斌,李彦林,李晓林,等.骨关节炎的转基因动物模型 [J].中国组织工程研究与临床康复,2011,15(7):1269-1272.
[25] Griffin TM,Huebner JL,Kraus VB,et al.Induction of osteoarthritis and metabolic inflammation by a very high-fat diet in mice:effects of short-term exercise [J].Arthritis Rheum,2014,64(2):443-453.
[26] Louer CR,Furman BD,Huebner JL,et al.Diet-induced obesity significantly increases the severity of posttraumatic arthritis in mice [J].Arthritis Rheum,2012,64(10):3220-3230.
[27] Asou Y,Iwata M,Ochi H,et al.Pleiotropic functions of high fat diet in the etiology of osteoarthritis [J].PLoS One,2016,11(9):e0162794.
[28] 马驯.软骨下骨在Hartley豚鼠自发性骨性关节炎的发生与发展中属性变化的基础研究 [D].中国医科大学,2018.
[29] Marijnissen AC,van Roermund PM,Tekoppele JM,et al.The canine groove model,compared with the ACLT model of osteoarthritis [J].Osteoarthr Cartil,2002,10(2):145-155.
[30] 罗春海,刘健莹,刘瑶,等.高频超声对试验性犬膝关节骨关节炎诊断意义 [J].中国兽医学报,2018,38(1):189-194.
[31] 彭诗,刘娟,朱兆荣,等.电针对木瓜蛋白酶致骨关节炎模型犬血清中IL-1、TNF-α、SP的影响 [J].中国兽医杂志,2014,50(6):52-54.
[32] 王斌,邢丹,董圣杰,等.中国膝骨关节炎流行病学和疾病负担的系统评价 [J].中国循证医学杂志,2018,18(2):134-142.
[33] Fischenich K,Pauly H,Button K,et al.A study of acute and chronic tissue changes in surgical and traumatically-induced experimental models of knee joint injury using magnetic resonance imaging and micro-computed tomography [J].Osteoarthr Cartil,2017,25(4):561-569.
[34] 王文胜,陈伟,沈堡垒,等.上、下坡跑台训练膝骨关节炎模型大鼠关节软骨及炎症因子的变化 [J].中国组织工程研究,2016,20(2):197-201.
[35] Ou YS,Tan C,An H,et al.The effects of NSAIDs on types I,II,and III collagen metabolism in a rat osteoarthritis model [J].Rheumatol Int,2012,32(8):2401-2405.
[36] 黄肖华,段戡,袁长深,等.结扎股静脉对兔膝关节软骨组织形态学的影响 [J].中医正骨,2010,22(8):16-18.
[37] 戴子一.基于1HNMR平台探讨揉髌手法对兔膝关节骨关节炎模型血清代谢组学的影响 [D].广西中医药大学,2017.
[38] 尼加提·外力,居来提·买提肉孜,李艳娜,等.沙疗温度对骨重建实验的影响及传热数值模拟研究 [J].中国实验动物学报,2017,25(2):181-185.
[39] 王维山,史晨辉,周卓浩,等.一侧兔膝关节注射外源性尿激酶型纤溶酶原激活物引起双侧膝关节软骨降解的实验 [J].中国组织工程研究与临床康复,2007,11(19):3738-3741.
[40] Malfait AM,Tortorella M,Thompson J,et al.Intra-articular injection of tumor necrosis factor-α in the rat:an acute and reversible in vivo model of cartilage proteoglycan degradation [J].Osteoarthritis Cartilage,2009,17(5):627-635.
[41] 马玉峰,祁印泽,王庆甫,等.关节内注射药物建立骨性关节炎动物模型研究进展 [J].中国骨伤,2015,28(1):90-95.
[42] 何名江,张洪美,荆琳,等.骨关节炎动物模型:谁最适宜你的研究?[J].中国组织工程研究,2013,17(46):8069-8074.
[2] 郭义.实验针灸学 [M].北京:中国中医药出版社,2016:19.
[3] 蒋洋洋,朱浩,张海.人类与小鼠同源lncRNA基因及其表观遗传调控靶基因分析 [J].南方医科大学学报,2018,38(6):731-735.
[4] 曾俊华,马笃军,彭力平,等.实验兔膝骨关节炎模型的建立及鉴定 [J].中国临床研究,2016,29(5):679-682.
[5] 尚平,贺宪,陈孝银,等.过伸位和过屈位固定复制骨性关节炎动物模型的比较 [J].生物骨科材料与临床研究,2006,3(1):11-14.
[6] 陈达,彭力平,廖州伟,等.木瓜蛋白酶与石膏制动建立兔膝骨关节炎模型的比较 [J].广东医学,2017,38(14):2114-2118.
[7] Hermeto LC,Derossi R,Oliveira RJ,et al.Effects of intra-articular injection of mesenchymal stem cells associated with platelet-rich plasma in a rabbit model of osteoarthritis [J].Genet Mol Res,2016,15(3).
[8] Hulth A,Lindberg L,Telhag H.Experimental osteoarthritis in rabbits.Preliminary report [J].Acta Orthop Scand,1970,41(5):522-530.
[9] 熊元,赵振国,李传郡,等.兔膝骨关节炎模型的制备及鉴定 [J].实验动物科学,2013,30(3):31-34,63.
[10] Roman-Blas JA,Mediero A,Tardío L,et al.The combined therapy with chondroitin sulfate plus glucosamine sulfate or chondroitin sulfate plus glucosamine hydrochloride does not improve joint damage in an experimental model of knee osteoarthritis in rabbits [J].Eur J Pharmacol,2017,794:8-14.
[11] 李炳辉,牛东生,孙玺淳,等.直立负重法与Hulth法建立兔膝骨关节炎模型的对比研究 [J].宁夏医科大学学报,2013,35(3):256-258,262.
[12] 黄吉利,罗明,潘捷,等.持续被动运动对兔骨关节炎软骨细胞凋亡相关基因P53、caspase-3、Bcl-2和Bax的影响 [J].中国老年学杂志,2016,36(24):6067-6069.
[13] 张冲,王恒树,阚秀莲,等.传统中药补肾固筋方对膝骨性关节炎IL-1、TNF-α的作用机制 [J].中国实验动物学报,2015,23(3):312-315.
[14] 钱洁,邢雪松,梁军.大鼠膝关节骨性关节炎动物模型的两种实验方案 [J].实验室研究与探索,2014,33(11):23-27.
[15] Kellyy S,Dunham JP,Murray F,et al.Spontaneous firing in C-fibers and increased mechanical sensitivity in A-fibers of knee joint-associated mechanoreceptive primary afferent neurones during MIA-induced osteoarthritis in the rat [J].Osteoarthr Cartil,2012,20(4):305-313.
[16] 汪宗保,徐亚林,廖威明,等.木瓜蛋白酶诱导大鼠膝早期骨关节炎软骨表面的电镜扫描 [J].中国组织工程研究,2014,18(2):177-182.
[17] 黄永明,潘建科,郭达,等.Ⅱ型胶原蛋白酶诱导SD大鼠膝骨关节炎模型的建立 [J].广东医学,2015,36(8):1145-1148.
[18] Salazar-Noratto GE,De Nijs N,Stevens HY,et al.Regional gene expression analysis of multiple tissues in an experimental animal model of post-traumatic osteoarthritis [J].Osteoarthr Cartil,2019,27(2):294-303.
[19] 李安琪,冯伟,何东仪,等.改良韧带缺损法建立大鼠膝骨关节炎模型 [J].上海中医药杂志,2018,52(5):13-17.
[20] Pernille HA,László BT,Thomas LA,et al.Ovariectomized rats as a model of postmenopausal osteoarthritis:validation and application [J].Arthritis Res Ther,2004,6(2):169-180.
[21] 谢靖,杨冠.一种新的半月板切断导致小鼠骨关节炎方法的建立 [J].军事医学,2013,37(9):708-711.
[22] van der Kraan PM,Vitters EL,van de Putte LB,et al.Development of osteoarthritic lesions in mice by “metabolic” and “mechanical” alterations in the knee joints [J].Am J Pathol,1989,135(6):1001-1014.
[23] Arita M,Li SW,Kopen G,et al.Skeletal abnormalities and ultrastructural changes of cartilage in transgenic mice expressing a collagen II gene (COL2A1) with a Cys for Arg-α1-519 substitution [J].Osteoarthr Cartil,2002,10(10):808-815.
[24] 曹斌,李彦林,李晓林,等.骨关节炎的转基因动物模型 [J].中国组织工程研究与临床康复,2011,15(7):1269-1272.
[25] Griffin TM,Huebner JL,Kraus VB,et al.Induction of osteoarthritis and metabolic inflammation by a very high-fat diet in mice:effects of short-term exercise [J].Arthritis Rheum,2014,64(2):443-453.
[26] Louer CR,Furman BD,Huebner JL,et al.Diet-induced obesity significantly increases the severity of posttraumatic arthritis in mice [J].Arthritis Rheum,2012,64(10):3220-3230.
[27] Asou Y,Iwata M,Ochi H,et al.Pleiotropic functions of high fat diet in the etiology of osteoarthritis [J].PLoS One,2016,11(9):e0162794.
[28] 马驯.软骨下骨在Hartley豚鼠自发性骨性关节炎的发生与发展中属性变化的基础研究 [D].中国医科大学,2018.
[29] Marijnissen AC,van Roermund PM,Tekoppele JM,et al.The canine groove model,compared with the ACLT model of osteoarthritis [J].Osteoarthr Cartil,2002,10(2):145-155.
[30] 罗春海,刘健莹,刘瑶,等.高频超声对试验性犬膝关节骨关节炎诊断意义 [J].中国兽医学报,2018,38(1):189-194.
[31] 彭诗,刘娟,朱兆荣,等.电针对木瓜蛋白酶致骨关节炎模型犬血清中IL-1、TNF-α、SP的影响 [J].中国兽医杂志,2014,50(6):52-54.
[32] 王斌,邢丹,董圣杰,等.中国膝骨关节炎流行病学和疾病负担的系统评价 [J].中国循证医学杂志,2018,18(2):134-142.
[33] Fischenich K,Pauly H,Button K,et al.A study of acute and chronic tissue changes in surgical and traumatically-induced experimental models of knee joint injury using magnetic resonance imaging and micro-computed tomography [J].Osteoarthr Cartil,2017,25(4):561-569.
[34] 王文胜,陈伟,沈堡垒,等.上、下坡跑台训练膝骨关节炎模型大鼠关节软骨及炎症因子的变化 [J].中国组织工程研究,2016,20(2):197-201.
[35] Ou YS,Tan C,An H,et al.The effects of NSAIDs on types I,II,and III collagen metabolism in a rat osteoarthritis model [J].Rheumatol Int,2012,32(8):2401-2405.
[36] 黄肖华,段戡,袁长深,等.结扎股静脉对兔膝关节软骨组织形态学的影响 [J].中医正骨,2010,22(8):16-18.
[37] 戴子一.基于1HNMR平台探讨揉髌手法对兔膝关节骨关节炎模型血清代谢组学的影响 [D].广西中医药大学,2017.
[38] 尼加提·外力,居来提·买提肉孜,李艳娜,等.沙疗温度对骨重建实验的影响及传热数值模拟研究 [J].中国实验动物学报,2017,25(2):181-185.
[39] 王维山,史晨辉,周卓浩,等.一侧兔膝关节注射外源性尿激酶型纤溶酶原激活物引起双侧膝关节软骨降解的实验 [J].中国组织工程研究与临床康复,2007,11(19):3738-3741.
[40] Malfait AM,Tortorella M,Thompson J,et al.Intra-articular injection of tumor necrosis factor-α in the rat:an acute and reversible in vivo model of cartilage proteoglycan degradation [J].Osteoarthritis Cartilage,2009,17(5):627-635.
[41] 马玉峰,祁印泽,王庆甫,等.关节内注射药物建立骨性关节炎动物模型研究进展 [J].中国骨伤,2015,28(1):90-95.
[42] 何名江,张洪美,荆琳,等.骨关节炎动物模型:谁最适宜你的研究?[J].中国组织工程研究,2013,17(46):8069-8074.