银鲳(Pampus argenteus)幼鱼饥饿前后侧囊及肝脏超微结构的变化
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摘要
分别采用扫描和透射电镜,观察了正常和饥饿5d银鲳(Pampus argenteus)幼鱼的食道侧囊及肝脏超微结构的变化。结果表明,正常银鲳幼鱼侧囊内壁具有许多平均高度为800 m的乳突,上有次级突起。乳突黏膜由复层扁平上皮构成,上皮细胞表面有指纹状的微嵴,有缓解上皮细胞的机械损伤作用;银鲳肝脏具肝血窦、胆小管、狄氏窦等结构,肝细胞排列较紧密,内含丰富的细胞器和脂滴,核仁大而明显,线粒体数目多,多分布在细胞核、内质网和狄氏窦周围。饥饿后,侧囊乳突变低矮,平均高度700 m,乳突上黏膜变薄,逐渐退缩,次级突起顶端露出了尖刺状的角质刺;肝细胞体积缩小,核变小,细胞器数目减少,线粒体水肿,粗面内质网数量显著降低,脂滴数量降低,这是银鲳幼鱼对饥饿的生理性防御。
The differences in ultrastructure changes of esophageal sac and liver in normal and 5d hunger juvenile of Pampus argenteus was observed in scanning and transmission electron microscopy.Result showsa number of mastoids in average height of 800μm in the inner wall of esophageal sac,on which the secondary mastoids were found in normal juvenile of P.argenteus.Mastoid mucosa consisted of a stratified squamous epithelium,with fingerprint-like microridges at the surface of epithelial cellsfor playing a role of mitigation to mechanical damage of epithelial cells.The liver cells of normal fish show textures of hepatic sinus,erythrocytes,microvilli,Dieldrin sinus,bile canaliculi,and mitochondria,Hepatic cell arranged closely,containing many organelles and lipid droplets,nucleoli were large and obvious.Many mitochondria located around the nucleus,endoplasmic reticulum,and Dieldrin sinus.After starvation,the mastoids in the inner wall of esophageal sac became lowered(average height 700μm),mastoid mucosa thinned and retreated gradually,during which sharp horny spine was exposed from the top of secondary mastoid.The structure of liver cells changed greatly after starvation:sizes of liver cells and nuclear shrank,the number of organelles reduced,mitochondria swelled,the number of rough endoplasmic reticulum and lipid droplets decreased significantly,which is a physiological defense against starvation by juvenile of P.argenteus.
The differences in ultrastructure changes of esophageal sac and liver in normal and 5d hunger juvenile of Pampus argenteus was observed in scanning and transmission electron microscopy.Result showsa number of mastoids in average height of 800μm in the inner wall of esophageal sac,on which the secondary mastoids were found in normal juvenile of P.argenteus.Mastoid mucosa consisted of a stratified squamous epithelium,with fingerprint-like microridges at the surface of epithelial cellsfor playing a role of mitigation to mechanical damage of epithelial cells.The liver cells of normal fish show textures of hepatic sinus,erythrocytes,microvilli,Dieldrin sinus,bile canaliculi,and mitochondria,Hepatic cell arranged closely,containing many organelles and lipid droplets,nucleoli were large and obvious.Many mitochondria located around the nucleus,endoplasmic reticulum,and Dieldrin sinus.After starvation,the mastoids in the inner wall of esophageal sac became lowered(average height 700μm),mastoid mucosa thinned and retreated gradually,during which sharp horny spine was exposed from the top of secondary mastoid.The structure of liver cells changed greatly after starvation:sizes of liver cells and nuclear shrank,the number of organelles reduced,mitochondria swelled,the number of rough endoplasmic reticulum and lipid droplets decreased significantly,which is a physiological defense against starvation by juvenile of P.argenteus.
引文
方静,谢林,李逊等,1995.齐口裂腹鱼消化道黏膜上皮的扫描电镜观察.水生生生学报,19(2):188—189
方展强,林敏朝,2006.剑尾鱼肝脏结构的光镜和透射电镜观察.电子显微学报,25(3):265—270
方展强,郑文彪,符路娣,2002.苏氏鱼芒鲶肝脏的超微结构.中国水产科学,9(4):296—299
方展强,符路娣,郑文彪,2003.不同生殖期鳜肝脏超微结构变化的观察.水生生物学报,27(6):64—70
付世建,邓利,张文兵等,1999.南方鲇幼鱼胃和肝脏的组织结构及其在饥饿过程中的变化.西南师范大学学报(自然科学版),24(3):336—342
刘静,李春生,李显森,2002.中国鲳属鱼类的分类研究.海洋科学集刊,44:240—251
李霞,姜志强,谭晓珍等,2002.饥饿和再投喂对美国红鱼消化器官组织学的影响.中国水产科学,9(3):211—215
李加儿,区又君,刘匆,2009.斑点叉尾鳃结构的扫描电镜观察.南方水产,5(5):52—56
李玉和,郭淑华,1992.乌鳢消化道黏膜的扫描电镜研究.解剖学报,23(1):98—101
宋昭彬,何学福,2000.饥饿对南方鲇仔稚鱼消化系统的形态和组织学影响.水生生物学报,24(2):155—160
宋海霞,翁幼竹,方琼珊等,2008.斜带石斑鱼幼鱼饥饿前后胃肠道的组织学研究.台湾海峡,27(1):74—78
张守信,纳索尔J R,1990.金鱼、八目鳗和蟾蜍蝌蚪表皮Malpinghian细胞超微结构.动物学报,2:110—113
林仕梅,罗莉,叶元土,2003.黄颡鱼、大鳍消化道黏膜的扫描电镜观察.四川动物,22(2):63—65
施兆鸿,马凌波,高露姣等,2007.人工育苗条件下银鲳仔稚幼鱼摄食与生长特性.海洋水产研究,28(4):38—47
徐豪,马淑懿,1980.几种淡水养殖鱼类肝脏组织的细胞结构和亚显微结构.中山大学学报(自然科学版),(3):93—99
徐善良,王丹丽,徐继林等,2012.东海银鲳(Pampus argenteus)、灰鲳(P.cinereus)和中国鲳(P.sinensis)肌肉主要营养成分分析与评价.海洋与湖沼,43(4):775—781
殷帅文,林学群,陈洁辉,2008.鲮鱼肝细胞超微结构研究.水生态学杂志,1(2):85—89
殷帅文,林学群,陈洁辉,2011.饥饿和再恢复投喂对鲻鱼肝细胞超微结构的影响.井冈山大学学报(自然科学版),32(3):60—64
高露姣,施兆鸿,严莹,2007.银鲳仔鱼消化系统的组织学研究.中国水产科学,14(4):540—544
郭琼林,卢全章,1994.鳗鲡肝脏、脾脏显微与超微结构.动物学报,40(2):125—130
黄旭雄,施兆鸿,李伟微等,2009.银鲳亲鱼不同组织的氨基酸及其随性腺发育的变化.水产学报,33(2):278—287
黄建华,李加儿,刘匆等,2005.鲑点石斑鱼和大眼鳜鳃的扫描电镜观察.动物学研究,26(1):82—88
崔龙波,周雪莹,陆瑶华,2002.皱纹盘鲍食道的结构与功能.水产学报,26(1):7—14
彭士明,施兆鸿,孙鹏等,2010.养殖密度对银鲳幼鱼生长及组织生化指标的影响.生态学杂志,7:1371—1376
彭士明,施兆鸿,李杰等,2011.运输胁迫对银鲳血清皮质醇、血糖、组织中糖元及乳酸含量的影响.水产学报,35(6):831 —837
Arilo A,Gaino E,Mragiocco C et al,1984.Biochemical and ultrastructural effects of nitrite in rainbow trout:liver hypoxia as the root of the acute toxicity mechanism.Env Res,34:135—154
Bisbal G A,Bengtson D A,1995.Description of starving condition in summer flounder Paralichthys dentatus early history stage.Fish Bulletin,93:217—230
Bonates A B,Ferri S,1980.Fine structure of a fresh water Teleost(Pimelodus maculatus)hepatocytes revealed by ultrathin sections.Anat Anz,148:132—144
Calaldi E,1987.A study of the histology and morphology of the digestive tract of the sea-bream Sparus aurata.J Fish Biol,30(2):135—145
Ehrlish K,Blaxter J,1976.Morphological and histological changes during growth and starvation of Herring and plaice larvae.Mar Biol,35:105—108
Ezeasor D N,Stokoe W M,1980.Scanning electron microscopic study of the gut mucosa of the rainbow trout Saimo gairdneri Richardson.J Fish Biol,17:529—539
Fretter V,Graham A,1962.British Prosobranch Mollusks.London:Ray Society,207—222
Kjorsvik E,Meeren T V,Kryvi H et al,1991.Early development of the digestive tract of food larvae,Gadus morhua L.,during start-feeding and starvation.Journal of Fish Biology,38:
MacLeod M G,1978.Effects of salinity and starvation on the alimentary canal anatomy of the rainbow trout,Salmo gairdneri Richardson.J Fish Biol,12:71—79
Storch V,Juario J V,1983.The effect of starvation and subsequent feeding on the hepatopancreas of chanos(Forsskal)fingerlings and fry.Journal of Fish Biology,23(1):95—103
Weisel G F,1973.Anatomy and histology of the digestive system of the paddle fish(Polyodon spathula).J Morph,140:243—256
方展强,林敏朝,2006.剑尾鱼肝脏结构的光镜和透射电镜观察.电子显微学报,25(3):265—270
方展强,郑文彪,符路娣,2002.苏氏鱼芒鲶肝脏的超微结构.中国水产科学,9(4):296—299
方展强,符路娣,郑文彪,2003.不同生殖期鳜肝脏超微结构变化的观察.水生生物学报,27(6):64—70
付世建,邓利,张文兵等,1999.南方鲇幼鱼胃和肝脏的组织结构及其在饥饿过程中的变化.西南师范大学学报(自然科学版),24(3):336—342
刘静,李春生,李显森,2002.中国鲳属鱼类的分类研究.海洋科学集刊,44:240—251
李霞,姜志强,谭晓珍等,2002.饥饿和再投喂对美国红鱼消化器官组织学的影响.中国水产科学,9(3):211—215
李加儿,区又君,刘匆,2009.斑点叉尾鳃结构的扫描电镜观察.南方水产,5(5):52—56
李玉和,郭淑华,1992.乌鳢消化道黏膜的扫描电镜研究.解剖学报,23(1):98—101
宋昭彬,何学福,2000.饥饿对南方鲇仔稚鱼消化系统的形态和组织学影响.水生生物学报,24(2):155—160
宋海霞,翁幼竹,方琼珊等,2008.斜带石斑鱼幼鱼饥饿前后胃肠道的组织学研究.台湾海峡,27(1):74—78
张守信,纳索尔J R,1990.金鱼、八目鳗和蟾蜍蝌蚪表皮Malpinghian细胞超微结构.动物学报,2:110—113
林仕梅,罗莉,叶元土,2003.黄颡鱼、大鳍消化道黏膜的扫描电镜观察.四川动物,22(2):63—65
施兆鸿,马凌波,高露姣等,2007.人工育苗条件下银鲳仔稚幼鱼摄食与生长特性.海洋水产研究,28(4):38—47
徐豪,马淑懿,1980.几种淡水养殖鱼类肝脏组织的细胞结构和亚显微结构.中山大学学报(自然科学版),(3):93—99
徐善良,王丹丽,徐继林等,2012.东海银鲳(Pampus argenteus)、灰鲳(P.cinereus)和中国鲳(P.sinensis)肌肉主要营养成分分析与评价.海洋与湖沼,43(4):775—781
殷帅文,林学群,陈洁辉,2008.鲮鱼肝细胞超微结构研究.水生态学杂志,1(2):85—89
殷帅文,林学群,陈洁辉,2011.饥饿和再恢复投喂对鲻鱼肝细胞超微结构的影响.井冈山大学学报(自然科学版),32(3):60—64
高露姣,施兆鸿,严莹,2007.银鲳仔鱼消化系统的组织学研究.中国水产科学,14(4):540—544
郭琼林,卢全章,1994.鳗鲡肝脏、脾脏显微与超微结构.动物学报,40(2):125—130
黄旭雄,施兆鸿,李伟微等,2009.银鲳亲鱼不同组织的氨基酸及其随性腺发育的变化.水产学报,33(2):278—287
黄建华,李加儿,刘匆等,2005.鲑点石斑鱼和大眼鳜鳃的扫描电镜观察.动物学研究,26(1):82—88
崔龙波,周雪莹,陆瑶华,2002.皱纹盘鲍食道的结构与功能.水产学报,26(1):7—14
彭士明,施兆鸿,孙鹏等,2010.养殖密度对银鲳幼鱼生长及组织生化指标的影响.生态学杂志,7:1371—1376
彭士明,施兆鸿,李杰等,2011.运输胁迫对银鲳血清皮质醇、血糖、组织中糖元及乳酸含量的影响.水产学报,35(6):831 —837
Arilo A,Gaino E,Mragiocco C et al,1984.Biochemical and ultrastructural effects of nitrite in rainbow trout:liver hypoxia as the root of the acute toxicity mechanism.Env Res,34:135—154
Bisbal G A,Bengtson D A,1995.Description of starving condition in summer flounder Paralichthys dentatus early history stage.Fish Bulletin,93:217—230
Bonates A B,Ferri S,1980.Fine structure of a fresh water Teleost(Pimelodus maculatus)hepatocytes revealed by ultrathin sections.Anat Anz,148:132—144
Calaldi E,1987.A study of the histology and morphology of the digestive tract of the sea-bream Sparus aurata.J Fish Biol,30(2):135—145
Ehrlish K,Blaxter J,1976.Morphological and histological changes during growth and starvation of Herring and plaice larvae.Mar Biol,35:105—108
Ezeasor D N,Stokoe W M,1980.Scanning electron microscopic study of the gut mucosa of the rainbow trout Saimo gairdneri Richardson.J Fish Biol,17:529—539
Fretter V,Graham A,1962.British Prosobranch Mollusks.London:Ray Society,207—222
Kjorsvik E,Meeren T V,Kryvi H et al,1991.Early development of the digestive tract of food larvae,Gadus morhua L.,during start-feeding and starvation.Journal of Fish Biology,38:
MacLeod M G,1978.Effects of salinity and starvation on the alimentary canal anatomy of the rainbow trout,Salmo gairdneri Richardson.J Fish Biol,12:71—79
Storch V,Juario J V,1983.The effect of starvation and subsequent feeding on the hepatopancreas of chanos(Forsskal)fingerlings and fry.Journal of Fish Biology,23(1):95—103
Weisel G F,1973.Anatomy and histology of the digestive system of the paddle fish(Polyodon spathula).J Morph,140:243—256