摘要
[目的]分析南丹牛的Y染色体遗传多样性及父系起源。[方法]用PCR扩增、测序及生物信息学方法进行分析。[结果]通过对25头南丹牛的Y-SNPs和Y-STRs分析,发现南丹牛仅包含瘤牛Y3单倍型组,细分为Y3-88-156和Y3-90-156两种单倍型,单倍型频率分别为92%和8%,南丹牛的Y染色体单倍型多样度为0.1533±0.0915。[结论]南丹牛是瘤牛父系起源,其遗传基础很稳定。
[Objective]The aim of this paper was to analyze Y chromosome genetic diversity and the paternal origin of Nandan cattle. [Methods] Polymerase chain reaction amplification, sequencing and bioinformatics were used for this study. [Results] Based on the analysis of Y-SNPs and Y-STRs markers of 25 Nandan cattle male samples, the results indicated that the Nandan cattle belonged to only zebu Y3 haplogroup,was divided into two haplotypes: Y3-88-156 and Y3-90-156,and their haplotype frequencies were 92% and 8%, respectively.The Y chromosome haplotypediversity was 0.1533 ± 0.0915 in Nandan cattle.[Conclusion] Nandan cattle belong to the zebu paternal origin with low paternal genetic diversity, indicating a very stable genetic basis.
引文
[1] 国家畜禽遗传资源委员会.中国畜禽遗传资源志:牛志[M].北京:中国农业出版社,2011.
[2] 陆维和,磨考诗,吴柱月,等.浅谈广西地方黄牛品种资源的现状与对策[C]//中国畜牧业协会,西北农林科技大学,中国畜牧业协会养牛学分会,中国良种黄牛育种委员会.第2届中国牛业发展大会论文集.北京:中国畜牧业协会,2007.
[3] 李冉,常振华,徐苹,等.黄牛Y染色体分子遗传多样性研究进展[J].中国牛业科学,2012,38(4):43-47.
[4] Edwards C J,Ginja C,Kantanen J,et al.Dual origins of dairy cattle farming-evidence from a comprehensive survey of European Y-chromosomal variation[J].Plos One,2011,6(1):e15922.
[5] 侯佳雯,夏小婷,贾玉堂,等.皖南牛Y-STRs与Y-SNPs遗传多样性研究[J].中国牛业科学,2018,44(1):30-32.
[6] Gōtherstrōm A,Anderung C,Hellborg L,et al.Cattle domestication in the Near East was followed by hybridization with aurochs bulls in Europe[J].Proceedings of the Royal Society B Biological Sciences,2005,272(1579):2345.
[7] 顿珠加才,张俸伟,夏小婷,等.西藏牛和日喀则驼峰牛Y-SNPs遗传多样性及父系起源研究[J].中国牛业科学,2017,43(6):7-9.
[8] 雷初朝,陈宏,胡沈荣.Y染色体多态性与中国黄牛起源和分类研究[J].西北农业学报,2000,9(4):43-47.
[9] Li R,Zhang X M,Campana M G,et al.Paternal origins of Chinese cattle[J].Animal Genetics,2013,44(4):446-449.
[10] 李芳玉,夏小婷,贾玉堂,等.大别山牛Y-STRs与Y-SNPs遗传多样性及父系起源研究[J].中国牛业科学,2018,44(2):4-6.
[11] 李铭,陈芳云,刘瑞鑫.广西地方黄牛优良品种保种与开发利用问题探讨[C]//中国畜牧业协会,西北农林科技大学,中国畜牧业协会养牛学分会,中国良种黄牛育种委员会.第3届中国牛业发展大会论文集.北京:中国畜牧业协会,2008.