丹艾痛经贴的提取工艺研究
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摘要
目的建立丹艾痛经贴的最佳提取工艺。方法采用超临界CO_2提取法提取肉桂、香附、艾叶挥发油,正交试验筛选最优提取工艺;采用高效液相法测定醇提药材中丹参酮ⅡA与丹酚酸B含量,以二者为指标进行正交试验优选最佳工艺。结果优选肉桂最佳提取条件为提取压力35 MPa、提取温度40℃、CO_2流量30 kg/h。香附最佳工艺条件为温度45℃、提取压力30 MPa、CO_2流量5.5 kg/h。艾叶最佳工艺条件为提取压力为20MPa,提取温度为30℃,CO_2流量24 kg/h;正交试验优选最佳醇提条件为10倍量75%乙醇,回流提取3次,每次1.5 h。结论本实验优选提取工艺简便,重复性好且准确可靠,可为中药贴剂的制备提供参考。
Objective To establish the optimal extraction process of Danai Dysmenorrhea Paste. Methods The volatile oil was extracted from Cinnamomi cortex, Cyperi rhizoma and Artemisiae argyi folium by supercritical CO_2 extraction. The optimal extraction process was screened by an orthogonal experiment with the contents of tanshinone Ⅱ A and salvianolic acid B in alcohol extract determined by HPLC as indexes. Results The optimal extraction conditions for Cinnamomi cortex were the extraction pressure of 35 MP, extraction temperature of 40 ℃ and CO_2 flow rate of 30 kg/h. The optimal extraction conditions for Cyperi rhizoma were the extraction pressure of 30 MPa, extraction temperature of 45 ℃ and CO_2 flow rate of 5.5 kg/h. The optimal extraction conditions for Artemisiae argyi folium were the extraction pressure of 20 MPa, extraction temperature of 30 ℃ and CO_2 flow rate of 24 kg/h. The optimal conditions of ethanol extraction were reflux extraction for 3 times with 10-fold of 75 % ethanol, 1.5 h each time. Conclusion The optimal extraction process can be simple, repeatable and accurate, providing reference for the preparation of traditional Chinese medicine patch.
Objective To establish the optimal extraction process of Danai Dysmenorrhea Paste. Methods The volatile oil was extracted from Cinnamomi cortex, Cyperi rhizoma and Artemisiae argyi folium by supercritical CO_2 extraction. The optimal extraction process was screened by an orthogonal experiment with the contents of tanshinone Ⅱ A and salvianolic acid B in alcohol extract determined by HPLC as indexes. Results The optimal extraction conditions for Cinnamomi cortex were the extraction pressure of 35 MP, extraction temperature of 40 ℃ and CO_2 flow rate of 30 kg/h. The optimal extraction conditions for Cyperi rhizoma were the extraction pressure of 30 MPa, extraction temperature of 45 ℃ and CO_2 flow rate of 5.5 kg/h. The optimal extraction conditions for Artemisiae argyi folium were the extraction pressure of 20 MPa, extraction temperature of 30 ℃ and CO_2 flow rate of 24 kg/h. The optimal conditions of ethanol extraction were reflux extraction for 3 times with 10-fold of 75 % ethanol, 1.5 h each time. Conclusion The optimal extraction process can be simple, repeatable and accurate, providing reference for the preparation of traditional Chinese medicine patch.
引文
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[3]张庆,樊奕琛,万朝霞,等.中医外治法治疗原发性痛经研究进展[J].中医临床研究, 2017, 9(12):140-141.
[4]胡永慧,佘一鸣,韩立云. 818中药透皮贴剂的临床应用进展[J].中草药, 2017, 48(13):2787-2792.
[5]黄彪,刘雁,陈彦,等.超临界二氧化碳提取肉桂精油的研究[J].林产化学与工业, 2003, 23(1):59-62.
[6]陈振华,宋必卫,苏香萍,等.香附的二氧化碳超临界提取工艺及其镇痛活性研究[J].医药导报, 2006, 25(3):192-194.
[7]王小生,熊维政,李静. CO2超临界萃取法提取艾叶挥发油工艺研究[J].中医学报, 2017, 32(9):170.
[8]姜琼,邹盛勤,周伟华,等.肉桂总多酚的提取工艺优选及降糖作用考察[J].中国实验方剂学杂志, 2013, 19(20):21-23.
[9]林三清,周中流,张华林,等.香附挥发油的提取工艺及其化学成分研究[J].岭南师范学院学报, 2017, 38(3):52-62
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[11]张玉,武一玫,潘真真,等.乌药总生物碱提取工艺的优选及镇痛作用的研究[J].药物评价研究, 2012, 35(5):348-350.
[12]黄一平,贾晓斌,施亚芳.养肝颗粒中延胡索提取工艺探讨[J].时珍国医国药, 1999, 10(11):818.