解磷菌的分离、筛选、鉴定及解磷能力研究
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摘要
为开发高效微生物解磷肥,利用解磷菌选择培养基(蒙吉娜卵磷脂培养基)从陕西省西安市周至县猕猴桃园农田土壤中分离出11株解磷菌,通过纯化培养,筛选出1株高效解磷菌JYP9。利用16S rDNA基因序列分析方法对该菌株的分类信息进行鉴定,鉴定结果表明该菌株为假单胞菌(Pseudomonas extremorientalis)。并用解磷圈法和液体摇瓶培养法,分别以卵磷脂为惟一磷源,确定了该菌株的最适培养温度为26℃、最适转速为200 r/min、最适起始pH为7和最适起始接种量为2%。
In order to develop an efficient microbial fertilizer solution, 11 strains from the farm-land soil of actinidia park in Zhouzhi county, Shaanxi prvince, were isolated, using phosphate volubility bacteria selective culture medium(Mongolia lecithin culture medium). A strain of phosphate volubility bacteria JYP9 was screened through purification cultivar. And classification information of this strain was identified by using 16 S r DNA gene sequence analysis, the results showed that the strain was a Pseudomonas extremorientalis. Then the phosphate solubilizing method and the liquid shake flask method were used in the experiment to confirm the optimum cultivation condition of JYP9 with lecithin as the only phosphorus source. The results showed that the optimal temperature was 26 ℃, optimal rotational was 200 r/min, optimal pH was 7, and optimum initial inoculation amount was 2%.
In order to develop an efficient microbial fertilizer solution, 11 strains from the farm-land soil of actinidia park in Zhouzhi county, Shaanxi prvince, were isolated, using phosphate volubility bacteria selective culture medium(Mongolia lecithin culture medium). A strain of phosphate volubility bacteria JYP9 was screened through purification cultivar. And classification information of this strain was identified by using 16 S r DNA gene sequence analysis, the results showed that the strain was a Pseudomonas extremorientalis. Then the phosphate solubilizing method and the liquid shake flask method were used in the experiment to confirm the optimum cultivation condition of JYP9 with lecithin as the only phosphorus source. The results showed that the optimal temperature was 26 ℃, optimal rotational was 200 r/min, optimal pH was 7, and optimum initial inoculation amount was 2%.
引文
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[15]杨艳华.土壤解磷微生物的分离鉴定[D].郑州:河南农业大学,2014.
[16]唐勇,陆玲,杨启银,等.解磷微生物及其应用的研究进展[J].天津农业科学,2001,7(2):1-5.
[2] DALAL R C. Soil organic phosphorus[J].Advances in Agronomy,1977,29(1):83-117.
[3]张宝贵,李贵桐.土壤生物在土壤磷有效化中的作用[J].土壤学报,1977,35(1):104-111.
[4]冯月红,姚拓,龙瑞军.土壤解磷菌研究进展[J].草原与草坪,2003,15(1):3-7.
[5]钟传青,黄为一.磷细菌P17对不同来源磷矿粉的溶磷作用及机制[J].土壤学报,2004,41(6):931-937.
[6] ILLMER P,BARBARA A,SCH-INNER F. Volubility of hardlysoluble AlPO4with P-volubility microorganisms[J].Soil Biochemistry,1995,27(3):265-270.
[7] SPERBER J I. Solution of mineral phosphates by soil bacteria[J]Nature,1957,180(1):994-995.
[8]张云翼,邹碧莹.土壤解磷细菌的研究进展[J].现代农业科技,2008(1):182-184.
[9]刘江,谷洁,高华,等.秦岭山区无机磷细菌筛选及其Biolog和分子生物学鉴定[J].干旱地区农业研究,2012,30(1):184-189.
[10]段秀梅,高晓蓉,吕军,等.两株土壤分离菌的解磷能力及对玉米的促生作用[J].中国土壤与肥料,2010,29(2):79-85.
[11]虞伟斌,杨兴明,沈其荣,等.K3解磷菌的解磷机理及其对缓冲容量的响应[J].植物营养与肥料学报,2010,16(2):354-361.
[12]胡秀芳,陈集双,陈海敏.一株有机磷降解菌的筛选、鉴定及其解磷功效[J].中国环境科学,2005,25(3):353-356.
[13]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科学技术出版社,2000.60-71.
[14]刘亚男,于水利,薛罡,等.聚磷菌PA01-1的筛选及除磷特性[J].中国给水排水,2005,21(10):13-17.
[15]杨艳华.土壤解磷微生物的分离鉴定[D].郑州:河南农业大学,2014.
[16]唐勇,陆玲,杨启银,等.解磷微生物及其应用的研究进展[J].天津农业科学,2001,7(2):1-5.