贡柑果园高效解磷菌的筛选及其解磷条件优化
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摘要
以广西钟山县贡柑果园的土壤为试材,从贡柑果园土壤中筛选出可以将难溶性或不溶性磷转化成可溶性磷的高效解磷菌;通过无机磷平板初筛和钼锑抗比色法复筛,经过6次传代获得一株高效解无机磷且遗传稳定的菌株W3;根据菌落形态特征、18S rDNA同源性分析,NCBI数据库对比分析等研究,初步鉴定为黄丝曲霉属(Talaromyces),并对菌株W3的解无机磷效果展开探究试验。结果表明:解磷菌W3解无机磷的最佳条件分别为接种量7%、培养温度30℃、摇床转速220 r·min~(-1)、培养时间7 d的条件下解无机磷效果最佳,最高可达到148.57 mg·L~(-1)。
Based on the soil of the Gonggan orchards of the Guangxi Zhongshan county,a high-efficiency phosphorous-solubilizing bacteria capable of converting insoluble or indissolvable into soluble phosphorus was selected by molybdenum-antimony colorimetric method.A high strain,through 6 passages,a highly stable solution of inorganic phosphorus and genetically stable strain W3 was obtained.According to strain W3 colony morphological characteristics,18S rDNA homology analysis,NCBI database comparative analysis and other studies,the preliminary identification strain W3 was Aspergillus flavus.The effect of inorganic phosphorus solution of strain W3 was investigated.The results showed that phosphorus bacteria W3 in the inoculation amount of 7%,culture temperature 30℃,shaking speed 220 r·min-1.Under the condition of 7 days,the optimal solution of inorganic phosphorus was 148.57 mg·L-1.
Based on the soil of the Gonggan orchards of the Guangxi Zhongshan county,a high-efficiency phosphorous-solubilizing bacteria capable of converting insoluble or indissolvable into soluble phosphorus was selected by molybdenum-antimony colorimetric method.A high strain,through 6 passages,a highly stable solution of inorganic phosphorus and genetically stable strain W3 was obtained.According to strain W3 colony morphological characteristics,18S rDNA homology analysis,NCBI database comparative analysis and other studies,the preliminary identification strain W3 was Aspergillus flavus.The effect of inorganic phosphorus solution of strain W3 was investigated.The results showed that phosphorus bacteria W3 in the inoculation amount of 7%,culture temperature 30℃,shaking speed 220 r·min-1.Under the condition of 7 days,the optimal solution of inorganic phosphorus was 148.57 mg·L-1.
引文
[1] 柯春亮,陈宇丰,周登博,等.香蕉根际土壤解磷细菌的筛选、鉴定及解磷能力[J].微生物学通报,2015,42(6):1032-1042.
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[13] 张云霞,雷鹏,许宗奇,等.一株高效解磷菌Bacillus subtilis JT-1的筛选及其对土壤微生态和小麦生长的影响[J].江苏农业报,2016,32(5):1073-1080.
[14] 姜瑛,吴越,王国文,等.一株固氮解磷菌的筛选鉴定及其对花生的促生作用研究[J].土壤,2015,47(4):698-703.
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[17] 杜雷,王素萍,陈钢,等.一株高效解磷细菌的筛选、鉴定及其溶磷能力的研究[J].中国土壤与肥料,2017(3):136-141.
[18] SPERBER J I.The incidence of apatite-solubilizinng organisms in the rhizosphere and soil[J].Crop & Pasture Science,1958,9(6):778-781.
[19] 吕睿,贾凤安,刘晨,等.无机磷降解菌株的分离、鉴定及解磷能力[J].江苏农业科学,2017,45(20):295-298.
[20] BASHAN Y,KAMNEV A A,DE-BASHAN L E.Tricalcium phosphate is inappropriate as a universal selection factor for isolating and testing phosphate-solubilizing bacteria that enhance plant growth:A proposal for an alternative procedure[J].Biol Fertil Soils,2013,49:465-479.
[21] 肖春玲,宋勇生,王安萍,等.菜园根际土壤解磷真菌的分离筛选及初步鉴定[J].井冈山大学学报(自然科学版),2012,33(4):48-51.
[22] LIU F P,LIU H Q,ZHOU H L,et al.Isolation and characterization of phosphate-solubilizing bacteria from betel nut (Areca catechu) and their effects on plant growth and phosphorus mobilization in tropical soils[J].Biol Fertil Soils,2014,50:927.
[2] 任友花,王羿超,李娜,等.微生物肥料高效解磷菌筛选及解磷机理探究[J].江苏农业科学,2016,44(12):537-540.
[3] 银婷婷,王敬敬,柳影,等.高效解磷菌的筛选及其促生机制的初步研究[J].生物技术通报,2015,31(12):234-242.
[4] 王雪,刘颖,辛雨,等.无机解磷真菌A2的分离鉴定及解磷条件的响应面优化[J].曲阜师范大学学报(自然科学版),2016,42(4):73-78.
[5] KHAN M,AHMAD E,ZAIDI A,etal.Bacteria in agrobiology:Crop productivity[M].Berlin Heidelberg:Springer,2013.
[6] YADAV B K,TARAFDAR J C.Penicillium purpurogenum,unique P mobilizers in arid agro-ecosystems[J].Arid Land Research and Management,2011,25(1):235-239.
[7] 蒙基娜 P A,陈淑筠.矿化有机磷化物的磷细菌[J].土壤学报,1953(3):200-203.
[8] 陈治宇,郎南军,李甜江.解磷微生物研究进展[J].绿色科技,2014(10):232-234.
[9] 魏伟,吴小芹,乔欢.马尾松根际高效解磷真菌的筛选鉴定及其促生效应[J].林业科学,2014,50(9):82-88.
[10] 田甜,沈丽英,施刘砚,等.含解磷菌螯合微量元素肥在番茄上的施用效果[J].磷肥与复肥,2017,32(3):39-41.
[11] 邢芳芳,高明夫,禚优优,等.玉米根际高效溶磷菌的筛选、鉴定及溶磷特性研究[J].中国农学通报,2016,32(9):119-124.
[12] 李学平,谢文军,范延辉,等.盐碱地塔宾曲霉菌的解磷能力及其对小麦生长的影响[J].水土保持通报,2017,37(1):93-96.
[13] 张云霞,雷鹏,许宗奇,等.一株高效解磷菌Bacillus subtilis JT-1的筛选及其对土壤微生态和小麦生长的影响[J].江苏农业报,2016,32(5):1073-1080.
[14] 姜瑛,吴越,王国文,等.一株固氮解磷菌的筛选鉴定及其对花生的促生作用研究[J].土壤,2015,47(4):698-703.
[15] 王亚艺.使用解磷细菌对小油菜产量及土壤磷含量的影响[J].北方园艺,2014(5):155-158.
[16] 郑喜清,邸娜,李旭红,等.土壤中解磷细菌的分离筛选[J].北方园艺,2017(9):164-168.
[17] 杜雷,王素萍,陈钢,等.一株高效解磷细菌的筛选、鉴定及其溶磷能力的研究[J].中国土壤与肥料,2017(3):136-141.
[18] SPERBER J I.The incidence of apatite-solubilizinng organisms in the rhizosphere and soil[J].Crop & Pasture Science,1958,9(6):778-781.
[19] 吕睿,贾凤安,刘晨,等.无机磷降解菌株的分离、鉴定及解磷能力[J].江苏农业科学,2017,45(20):295-298.
[20] BASHAN Y,KAMNEV A A,DE-BASHAN L E.Tricalcium phosphate is inappropriate as a universal selection factor for isolating and testing phosphate-solubilizing bacteria that enhance plant growth:A proposal for an alternative procedure[J].Biol Fertil Soils,2013,49:465-479.
[21] 肖春玲,宋勇生,王安萍,等.菜园根际土壤解磷真菌的分离筛选及初步鉴定[J].井冈山大学学报(自然科学版),2012,33(4):48-51.
[22] LIU F P,LIU H Q,ZHOU H L,et al.Isolation and characterization of phosphate-solubilizing bacteria from betel nut (Areca catechu) and their effects on plant growth and phosphorus mobilization in tropical soils[J].Biol Fertil Soils,2014,50:927.