针刺对乳腺增生大鼠乳腺组织Caspase-8mRNA和Caspase-3mRNA表达的影响
|
摘要
目的:探究针刺对乳腺增生大鼠乳腺组织中Caspase-8mRNA和Caspase-3mRNA表达的影响。方法:选取40只12周龄的健康雌性未孕SD大鼠(SPF级),将其随机分为正常组、模型组、针刺组与三苯氧胺组,每组10只。造模后进行4周的干预治疗,HE染色后光镜下观察各组大鼠乳腺组织病理状态,PCR法检测各组大鼠乳腺组织Caspase-8mRNA和Caspase-3mRNA的表达情况。结果:HE染色显示模型组较正常组出现乳腺小叶增多、腺泡结构紊乱等组织增生现象,Caspase-8mRNA、Caspase-3mRNA表达水平明显降低(P<0.01);与模型组相比,治疗后的针刺组与三苯氧胺组组织增生的病理变化减轻,Caspase-8mRNA、Caspase-3mRNA表达水平明显增高(P<0.01)。结论:针刺对乳腺增生大鼠乳腺组织中Caspase-8mRNA、Caspase-3mRNA表达有一定影响,可能通过干预凋亡通路对大鼠乳腺增生有调节和治疗作用。
Objective: To explore effects of acupuncture on Caspase-8 mRNA and Caspase-3 mRNA expressions of experimental hyperplasia rats' breast gland. Methods: We chose 40 female 12 weeks aged and non-pregnant SD rats and then randomly divided them into four groups: normal group,model group,acupuncture group,tamoxifen group. Each group had 10 rats. The treatment intervention was 4 weeks after the success of disease model. HE staining was used to observe the pathological changes of breast tissue. Real-time PCR technology was used to observe Caspase-8 mRNA and Caspase-3 mRNA expressions. Result:Compared with the normal group,lobuli mammae of the model group increased significantly. The disorder of acinar structure and tissue hyperplasia phenomenon were obvious. The expressions of Caspase-8 mRNA and Caspase-3 mRNA were significantly decreased( P < 0. 01). After treatment,the acupuncture group and tamoxifen group 's pathological changes of tissue hyperplasia were reduced compared with those of the model group. The expressions of Caspase-8 mRNA and Caspase-3 mRNA were significantly increased( P < 0. 01). Conclusion: Acupuncture can effect expressions of Caspase-8 mRNA and Caspase-3 mRNA in breast glands,regulate and treat hyperplasia of mammary glands in rats by intervening the apoptosis pathway.
Objective: To explore effects of acupuncture on Caspase-8 mRNA and Caspase-3 mRNA expressions of experimental hyperplasia rats' breast gland. Methods: We chose 40 female 12 weeks aged and non-pregnant SD rats and then randomly divided them into four groups: normal group,model group,acupuncture group,tamoxifen group. Each group had 10 rats. The treatment intervention was 4 weeks after the success of disease model. HE staining was used to observe the pathological changes of breast tissue. Real-time PCR technology was used to observe Caspase-8 mRNA and Caspase-3 mRNA expressions. Result:Compared with the normal group,lobuli mammae of the model group increased significantly. The disorder of acinar structure and tissue hyperplasia phenomenon were obvious. The expressions of Caspase-8 mRNA and Caspase-3 mRNA were significantly decreased( P < 0. 01). After treatment,the acupuncture group and tamoxifen group 's pathological changes of tissue hyperplasia were reduced compared with those of the model group. The expressions of Caspase-8 mRNA and Caspase-3 mRNA were significantly increased( P < 0. 01). Conclusion: Acupuncture can effect expressions of Caspase-8 mRNA and Caspase-3 mRNA in breast glands,regulate and treat hyperplasia of mammary glands in rats by intervening the apoptosis pathway.
引文
[1]谷丽艳,易佳丽,樊延宏,等.中医药疗法治疗乳腺增生研究进展[J].辽宁中医药大学学报,2014(1):173-176.
[2]王莹.针刺治疗乳腺增生病及其对乳腺组织影像和血流动力的影响[J].中国针灸,2005(9):619-621.
[3]张卫华,李青燕.针刺对MGH模型大鼠乳腺组织PCNA和BCL-2蛋白表达的影响[J].辽宁中医药大学学报,2008(9):153-154.
[4]贺妮娜.针灸推拿治疗乳腺增生的临床观察[J].世界最新医学信息文摘,2016(24):113-117.
[5]饶金才,李兰珍,陈云生,等.乳腺增生病动物模型的复制及病理类型[J].中国病理生理杂志,1992,8(6):671-672.
[6]李才.人类疾病动物模型的复制[M].北京:人民卫生出版社,2008:462-463.
[7]魏谭军,王毅,李霞,等.理气散结汤对大鼠乳腺增生的治疗作用及其机制[J].中国医院药学杂志,2017(7):603-607.
[8]林旭丰,严梅娣,谢小红,等.中医周期疗法对乳腺增生伴乳痛患者内分泌激素及症状的改善观察[J].中华中医药学刊,2017(3):745-748.
[9]李佳奕,张立德,谷丽艳.分析针刺治疗乳腺增生理论依据[J].辽宁中医药大学学报,2016(3):129-131.
[10]黄英.肺泡Ⅱ型上皮细胞凋亡在急性肺损伤中的作用[J].中国现代医学杂志,2002,12(3):35-37.
[11]Ferguson DJ,Anderson TJ.Morphological evaluation of cell turnover in relation to the menstrual cycle in the resting human breast[J].Br J Cancer,1981,44(2):177-181.
[12]Hu Qingliang,Cui Xinjie,Tao Lin,et al.Staphylococcus aureus induces apoptosis in primary bovine mammary epithelial cells through Fas-FADD death receptor-linked Caspase-8signaling[J].DNA Cell Biol,2014 33(6):388-397.
[13]Andreotti CS,Pereyra EA,Sacco SC,et al.Proliferation-apoptosis balance in Staphylococcus aureus chronically infected bovine mammary glands during involution[J].J Dairy Res,2017(1):1-9.
[14]赵相轩,温锋,卢再鸣.细胞凋亡在乳腺癌治疗中的研究进展[J].临床肿瘤学杂志,2017(2):175-179.
[15]刘春晓,石月萍.加减枳实薤白桂枝汤对大鼠心肌缺血再灌注损伤细胞凋亡及Caspase相关蛋白的影响[J].中药药理与临床,2017(1):11-14.
[2]王莹.针刺治疗乳腺增生病及其对乳腺组织影像和血流动力的影响[J].中国针灸,2005(9):619-621.
[3]张卫华,李青燕.针刺对MGH模型大鼠乳腺组织PCNA和BCL-2蛋白表达的影响[J].辽宁中医药大学学报,2008(9):153-154.
[4]贺妮娜.针灸推拿治疗乳腺增生的临床观察[J].世界最新医学信息文摘,2016(24):113-117.
[5]饶金才,李兰珍,陈云生,等.乳腺增生病动物模型的复制及病理类型[J].中国病理生理杂志,1992,8(6):671-672.
[6]李才.人类疾病动物模型的复制[M].北京:人民卫生出版社,2008:462-463.
[7]魏谭军,王毅,李霞,等.理气散结汤对大鼠乳腺增生的治疗作用及其机制[J].中国医院药学杂志,2017(7):603-607.
[8]林旭丰,严梅娣,谢小红,等.中医周期疗法对乳腺增生伴乳痛患者内分泌激素及症状的改善观察[J].中华中医药学刊,2017(3):745-748.
[9]李佳奕,张立德,谷丽艳.分析针刺治疗乳腺增生理论依据[J].辽宁中医药大学学报,2016(3):129-131.
[10]黄英.肺泡Ⅱ型上皮细胞凋亡在急性肺损伤中的作用[J].中国现代医学杂志,2002,12(3):35-37.
[11]Ferguson DJ,Anderson TJ.Morphological evaluation of cell turnover in relation to the menstrual cycle in the resting human breast[J].Br J Cancer,1981,44(2):177-181.
[12]Hu Qingliang,Cui Xinjie,Tao Lin,et al.Staphylococcus aureus induces apoptosis in primary bovine mammary epithelial cells through Fas-FADD death receptor-linked Caspase-8signaling[J].DNA Cell Biol,2014 33(6):388-397.
[13]Andreotti CS,Pereyra EA,Sacco SC,et al.Proliferation-apoptosis balance in Staphylococcus aureus chronically infected bovine mammary glands during involution[J].J Dairy Res,2017(1):1-9.
[14]赵相轩,温锋,卢再鸣.细胞凋亡在乳腺癌治疗中的研究进展[J].临床肿瘤学杂志,2017(2):175-179.
[15]刘春晓,石月萍.加减枳实薤白桂枝汤对大鼠心肌缺血再灌注损伤细胞凋亡及Caspase相关蛋白的影响[J].中药药理与临床,2017(1):11-14.