生白术对慢传输型便秘模型大鼠结肠水通道蛋白表达影响的研究
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摘要
目的:通过观察不同剂量生白术对慢传输型便秘(Slow transit constipation,STC)大鼠结肠动力及结肠水通道蛋白3(Aquaporin 3,AQP3)、水通道蛋白4 (Aquaporin 4,AQP4)表达的影响。方法:将50只健康SD大鼠随机分为空白组(A组)、模型组(B组)、白术低、中、高剂量组(C、D、E组)共5组。采用复方地芬诺酯混悬液按15 mg/(kg·d)的剂量灌胃建立STC大鼠模型,造模成功后,C、D、E组分别给予含生药3.15 g/kg,6.30 g/kg,9.45 g/kg的生白术水煎液干预治疗。通过比较大鼠一般情况、大便含水量、炭末推进率,观察不同剂量生白术治疗STC的疗效。采用免疫组化检测各组大鼠结肠组织中AQP3、AQP4分布及表达情况。结果:与A组比较,B组大鼠结肠炭末推进率、大便含水量及AQP3表达MD值降低(P <0.05),AQP4表达MD值升高(P <0.05);与B组比较,C组、D组、E组结肠炭末推进率、大便含水量及AQP3表达MD值均升高,仅D组、E组差异有统计学意义(P <0.05),AQP4表达MD值均降低(P<0.05)。结论:生白术可明显改善STC模型大鼠便秘症状,增加大便含水量、提高炭末推进率、促进肠道传输功能。白术通便存在一定的"剂量-效应"关系,中、高剂量作用优于低剂量。其作用机制可能与抑制结肠组织AQP4表达、增加AQP3表达有关。
Objective: To observe the effect of Rhizoma Atractylodis Macrocephalaee of different doses on the colonic motility as well as the expressions of Aquaporin 3(AQP3) and Aquaporin 4(AQP4) in the colon in rats with slow transit constipation(STC). Methods:A total of 50 healthy SD rats were randomly divided into five groups:the blank group(Group A),the model group(Group B), the Rhizoma Atractylodis Macrocephalaee groups of low-dose, medium-dose and high-dose(Group C,D and E). The rat models of STC were established by 15 mg/(kg·d) of compound diphenoxylate suspension?by gavage. After the successful establishment,Group C,D and E were respectively given the intervention treatment of water decoction containing crude drugs of 3.15 g/kg,6.30 g/kg and 9.45 g/kg of Rhizoma Atractylodis Macrocephalaee. The curative effect of Rhizoma Atractylodis Macrocephalaee of different doses in treating STC was observed by comparing the general condition,the water content of stool and the charcoal powder propelling rate of rats. The distribution and expression of AQP3 and AQP4 in the colon tissue of rats in each groups were detected by immunohistochemistry. Results:Compared with Group A, the charcoal powder propelling rate, the water content of stool and the mean density(MD) value in the expression of AQP3 in the colon in Group B was decreased(P < 0.05),and the MD value in the expression of AQP4 in the Group B was increased(P<0.05). Compared with Group B, the charcoal powder propelling rate, the water content of stool and the MD value in the expressions of AQP3 in the colon in Group C,D and E were increased,the difference being significant only in Group D and E(P < 0.05), and the MD value in the expression of AQP4 in Group C, D and E were decreased(P < 0.05).Conclusion:Rhizoma Atractylodis Macrocephalae can significantly improve the constipation symptoms of model rats with STC,increase the water content of stool,raise the charcoal powder propelling rate and promote the intestinal transmission function.There is a certain dose-effect relationship between Rhizoma Atractylodis Macrocephalae and catharsis, and the effect of medium-dose and high-dose is better than that of low-dose. Its mechanism may be related to the inhibition of the expression of AQP4 and the increase of the expression of AQP3 in colon tissue.
Objective: To observe the effect of Rhizoma Atractylodis Macrocephalaee of different doses on the colonic motility as well as the expressions of Aquaporin 3(AQP3) and Aquaporin 4(AQP4) in the colon in rats with slow transit constipation(STC). Methods:A total of 50 healthy SD rats were randomly divided into five groups:the blank group(Group A),the model group(Group B), the Rhizoma Atractylodis Macrocephalaee groups of low-dose, medium-dose and high-dose(Group C,D and E). The rat models of STC were established by 15 mg/(kg·d) of compound diphenoxylate suspension?by gavage. After the successful establishment,Group C,D and E were respectively given the intervention treatment of water decoction containing crude drugs of 3.15 g/kg,6.30 g/kg and 9.45 g/kg of Rhizoma Atractylodis Macrocephalaee. The curative effect of Rhizoma Atractylodis Macrocephalaee of different doses in treating STC was observed by comparing the general condition,the water content of stool and the charcoal powder propelling rate of rats. The distribution and expression of AQP3 and AQP4 in the colon tissue of rats in each groups were detected by immunohistochemistry. Results:Compared with Group A, the charcoal powder propelling rate, the water content of stool and the mean density(MD) value in the expression of AQP3 in the colon in Group B was decreased(P < 0.05),and the MD value in the expression of AQP4 in the Group B was increased(P<0.05). Compared with Group B, the charcoal powder propelling rate, the water content of stool and the MD value in the expressions of AQP3 in the colon in Group C,D and E were increased,the difference being significant only in Group D and E(P < 0.05), and the MD value in the expression of AQP4 in Group C, D and E were decreased(P < 0.05).Conclusion:Rhizoma Atractylodis Macrocephalae can significantly improve the constipation symptoms of model rats with STC,increase the water content of stool,raise the charcoal powder propelling rate and promote the intestinal transmission function.There is a certain dose-effect relationship between Rhizoma Atractylodis Macrocephalae and catharsis, and the effect of medium-dose and high-dose is better than that of low-dose. Its mechanism may be related to the inhibition of the expression of AQP4 and the increase of the expression of AQP3 in colon tissue.
引文
[1] BEKKALI N,DE JONGE H R,VAN DEN WIJINGAARD R M, et al. The role of rectal chloride secretion in childhood constipation[J].Neurogastroenterol Motil, 2011, 23(11):1007-1012.
[2]占煜,唐学贵.促分泌剂治疗慢性便秘的研究进展[J].中国新药杂志,2014,23(21):2509-2513.
[3]魏龙骧.医话四则[J].新医药学杂志,1978(4):9-10.
[4]冯广清.白术双向调节胃肠功能的初探[J].陕西中医函授,2001,18(1):24-25.
[5]吴鹏飞,顾勤.白术治疗功能性便秘及其机制的研究进展[J].世界华人消化杂志,2014,22(32):4934-4937.
[6]徐叔云,卞如濂,陈修.药理试验方法学[M].北京:人民卫生出版社,2002:1203.
[7]钱柏初,史红,郑晓亮.正常和便秘模型动物排便实验研究进展[J].中国临床药理学与治疗学,2007,12(12):1339-1343.
[8]丁曙晴,丁义江,张苏闽,等.白术水煎液治疗结肠慢传输性便秘36例疗效观察[J].新中医,2005,37(9):30-31.
[9]郑雪平.白术治疗结肠慢传输型便秘的研究[D].南京:南京中医药大学,2004.
[10]马慧萍,任宏颖.生白术治疗慢传输型便秘的护理体会[J].新疆中医药,2013,31(3):75-76.
[11]魏志军,张悦,张小慧,等.重用生白术治疗虚证便秘的临床及实验研究[J].中国中医药科技,2003,10(4):196-197.
[12]李希今.大剂量白术治疗便秘的临床验证[J].四川中医杂志,1994,7(3):29.
[13]朱冬雪.白术水煎液治疗结肠慢传输型便秘52例疗效观察[J].药物与人,2015,28(1):399.
[14]李超林.大剂量生白术合胃肠动力中药治疗老年性气虚型便秘65例[J].中医研究,2014,27(10):13-14.
[15]卜平,胡荣,卜乐.一种口服纳米生白术结肠片及其制备方法:200810021649.6[P].2009-01-07.
[16] LAFORENZA U.Water channel proteins in the gastrointestinal tract[J].Molecular Aspects of Medicine, 2012, 33(5-6):642-650.
[17] WANG K S,MA T,FILIZ F,et al.Colon water transport in transgenic mice lacking aquaporin-4 water channels[J].Am J Physiol Gastrointest Live Physiol, 2000, 279(2):G463-G470.
[2]占煜,唐学贵.促分泌剂治疗慢性便秘的研究进展[J].中国新药杂志,2014,23(21):2509-2513.
[3]魏龙骧.医话四则[J].新医药学杂志,1978(4):9-10.
[4]冯广清.白术双向调节胃肠功能的初探[J].陕西中医函授,2001,18(1):24-25.
[5]吴鹏飞,顾勤.白术治疗功能性便秘及其机制的研究进展[J].世界华人消化杂志,2014,22(32):4934-4937.
[6]徐叔云,卞如濂,陈修.药理试验方法学[M].北京:人民卫生出版社,2002:1203.
[7]钱柏初,史红,郑晓亮.正常和便秘模型动物排便实验研究进展[J].中国临床药理学与治疗学,2007,12(12):1339-1343.
[8]丁曙晴,丁义江,张苏闽,等.白术水煎液治疗结肠慢传输性便秘36例疗效观察[J].新中医,2005,37(9):30-31.
[9]郑雪平.白术治疗结肠慢传输型便秘的研究[D].南京:南京中医药大学,2004.
[10]马慧萍,任宏颖.生白术治疗慢传输型便秘的护理体会[J].新疆中医药,2013,31(3):75-76.
[11]魏志军,张悦,张小慧,等.重用生白术治疗虚证便秘的临床及实验研究[J].中国中医药科技,2003,10(4):196-197.
[12]李希今.大剂量白术治疗便秘的临床验证[J].四川中医杂志,1994,7(3):29.
[13]朱冬雪.白术水煎液治疗结肠慢传输型便秘52例疗效观察[J].药物与人,2015,28(1):399.
[14]李超林.大剂量生白术合胃肠动力中药治疗老年性气虚型便秘65例[J].中医研究,2014,27(10):13-14.
[15]卜平,胡荣,卜乐.一种口服纳米生白术结肠片及其制备方法:200810021649.6[P].2009-01-07.
[16] LAFORENZA U.Water channel proteins in the gastrointestinal tract[J].Molecular Aspects of Medicine, 2012, 33(5-6):642-650.
[17] WANG K S,MA T,FILIZ F,et al.Colon water transport in transgenic mice lacking aquaporin-4 water channels[J].Am J Physiol Gastrointest Live Physiol, 2000, 279(2):G463-G470.