宁夏男男性行为人群HIV-1感染者基因亚型及耐药分析
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摘要
目的了解宁夏男男性行为(MSM)人群HIV-1的流行亚型分布及耐药基因变异情况。方法采用方便抽样方法,抽取宁夏籍男男性行为人群HIV-1感染者共33份血浆标本,进行反转录/巢式PCR扩增HIV病毒的pol基因片段、DNA测序、亚型鉴定,并与国际HIV耐药数据库进行比对分析。结果宁夏MSM人群HIV-1毒株总体上未见明显的区域聚集性,33例样本呈现4种基因亚型分布:CRF07_BC、CRF01_AE、B、CRF55_01B,优势毒株为CRF07_BC(66.67%)和CRF01_AE(18.18%)亚型。CRF07_BC亚型序列主要形成2个基因簇。发现7例耐药突变株,全部为原发耐药,5例产生NRTIs与NNRTIs交叉耐药。结论宁夏MSM人群HIV-1流行毒株亚型分布较复杂,重组型毒株已成为优势流行株。原发耐药发生率高,交叉耐药明显,暂未见传播耐药株。
Objective To investigate the subtype distribution and gene mutations and drug resistance of human immunodeficiency virus(HIV)-1among men who have sex with men(MSM) in Ningxia.Methods A convenient sampling method was adopted to collect 33 plasma specimens from HIV-1 infected persons among MSM in Ningxia.And RNA was extracted and reverse transcription PCR and nested PCR for amplification of pol gene fragments,and DNA was sequenced and then phylogenetically analyzed.Finally subtypes were identified and drug resistance was analyzed in comparison with International HIV drug resistance database.Results In general,there was no obvious regional aggregation in the MSM HIV-1 strain in Ningxia.The samples presented in 33 samples showed 4 genotype subtypes:CRF07 _ BC,CRF01 _ AE,B,CRF55 _ 01 B,and the dominant strains were CRF07 _ BC(66.67%) and CRF01 _ AE(18.18%) subtypes.The CRF07_BC subtype sequence mainly formed 2 gene clusters.Seven drug resistant mutant strains were found,all of which were resistant to the drug,and 5 were the drug resistance of NRTIs and NNRTIs.Conclusion In the MSM population of Ningxia,the subtype distribution of HIV-1 popular strains is more complex,and the regroup strain has become an advantage.The incidence of drug resistance is high and the cross resistance is obvious,but no resistant strain is found.
Objective To investigate the subtype distribution and gene mutations and drug resistance of human immunodeficiency virus(HIV)-1among men who have sex with men(MSM) in Ningxia.Methods A convenient sampling method was adopted to collect 33 plasma specimens from HIV-1 infected persons among MSM in Ningxia.And RNA was extracted and reverse transcription PCR and nested PCR for amplification of pol gene fragments,and DNA was sequenced and then phylogenetically analyzed.Finally subtypes were identified and drug resistance was analyzed in comparison with International HIV drug resistance database.Results In general,there was no obvious regional aggregation in the MSM HIV-1 strain in Ningxia.The samples presented in 33 samples showed 4 genotype subtypes:CRF07 _ BC,CRF01 _ AE,B,CRF55 _ 01 B,and the dominant strains were CRF07 _ BC(66.67%) and CRF01 _ AE(18.18%) subtypes.The CRF07_BC subtype sequence mainly formed 2 gene clusters.Seven drug resistant mutant strains were found,all of which were resistant to the drug,and 5 were the drug resistance of NRTIs and NNRTIs.Conclusion In the MSM population of Ningxia,the subtype distribution of HIV-1 popular strains is more complex,and the regroup strain has become an advantage.The incidence of drug resistance is high and the cross resistance is obvious,but no resistant strain is found.
引文
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[10]王万海,明亮,姜树林,等.北京市男男性行为者中人类免疫缺陷病毒1的流行亚型及其变化趋势[J].中华传染病杂志,2012,30(11):673-676.
[11]吴健,王绪琴,尹鹤峰,等.2013年上海市男男性接触人群人类免疫缺陷病毒-1毒株耐药基因的变异[J].中华传染病杂志,2016,34(1):23-26.
[12]苏迎盈,刘慧鑫,吴静,等.中国HIV-1基因亚型分布及流行趋势[J].中华流行病学杂志,2014,35(10):1164-1168.
[13]吴忠兰,赵伟明,曹敏,等.宁夏回族自治区艾滋病合并丙型肝炎感染状况分析[J].中国艾滋病性病,2016,22(2):81-83.
[14]Zhao J,Cai WD,Zheng CL,et al.Origin and outbreak of HIV-1CRF55-01B among MSM in shenzhen,China[J].JAIDS Journal of Acquired Immune Deficiency Syndromes,2014,66(3):E65-E67.
[15]梁欣,彭晓霞.中国HIV感染者/AIDS患者服用抗病毒治疗药物后耐药性的系统综述[J].中国感染控制杂志,2016,15(4):254-257,261.
[16]曹慜,杨东智,吴忠兰,等.宁夏抗病毒治疗患者HIV基因型耐药及亚型分析[J].现代预防医学,2018,45(1):149-154.
[2]Ministry of Health of the People’s Republic of China.2012 China AIDS response progress report[R].Beijing:Other Organisations and Agencies,2012.
[3]赵立华,蒋岸,张蕴慧,等.宁夏男男性行为者HIV新发感染及影响因素分析[J].中国皮肤性病学杂志,2015,29(1):50-52.
[4]杨东智,吴忠兰,曹愍,等.2011年-2015年宁夏男男性行为哨点人群人类免疫缺陷病毒-1新发感染率调查研究[J].中国卫生检验杂志,2017,27(8):1164-1165,1184.
[5]Yang RS,Zhang C.Identification and characterization of a new class of human immunodeficiency virus type 1 recombinants comprised of two circulating recombinant forms,CRF07-BC and CRF08-BC,in China[J].Journal of Virology,2003,77:685-695.
[6]张佳峰,杨介者,潘晓红,等.浙江省男男性行为人群HIV-1新近感染者的毒株亚型和传播簇研究[J].中华流行病学杂志,2015,36(1):61-66.
[7]张海兰,闫永平,李恒新,等.西安市男男性行为人群感染HIV-1的分子流行病学研究[J].中华流行病学杂志,2014,35(4):421-424.
[8]郑敏娜,于茂河,宁铁林,等.天津市男男性接触人群人类免疫缺陷病毒-1感染者亚型与耐药性分析[J].中华传染病杂志,2010,28(5):303-308.
[9]于国龙,秦冰,鄢心革,等.广东男男性行为HIV-1感染者中耐药情况及基因型分布[J].分子诊断与治疗杂志,2015,7(3):156-160,198.
[10]王万海,明亮,姜树林,等.北京市男男性行为者中人类免疫缺陷病毒1的流行亚型及其变化趋势[J].中华传染病杂志,2012,30(11):673-676.
[11]吴健,王绪琴,尹鹤峰,等.2013年上海市男男性接触人群人类免疫缺陷病毒-1毒株耐药基因的变异[J].中华传染病杂志,2016,34(1):23-26.
[12]苏迎盈,刘慧鑫,吴静,等.中国HIV-1基因亚型分布及流行趋势[J].中华流行病学杂志,2014,35(10):1164-1168.
[13]吴忠兰,赵伟明,曹敏,等.宁夏回族自治区艾滋病合并丙型肝炎感染状况分析[J].中国艾滋病性病,2016,22(2):81-83.
[14]Zhao J,Cai WD,Zheng CL,et al.Origin and outbreak of HIV-1CRF55-01B among MSM in shenzhen,China[J].JAIDS Journal of Acquired Immune Deficiency Syndromes,2014,66(3):E65-E67.
[15]梁欣,彭晓霞.中国HIV感染者/AIDS患者服用抗病毒治疗药物后耐药性的系统综述[J].中国感染控制杂志,2016,15(4):254-257,261.
[16]曹慜,杨东智,吴忠兰,等.宁夏抗病毒治疗患者HIV基因型耐药及亚型分析[J].现代预防医学,2018,45(1):149-154.