摘要
基于已筛选出的鼠李糖脂产生菌M7-6,在模拟油藏条件(温度、pH、矿化度及缺氧)下,对该菌株的激活剂配方进行了碳源、氮源、碳氮比(C/N)、无机盐等因素的优化,并考察了该菌株在模拟油藏条件下的最佳接种量;利用厌氧发酵罐对菌株M7-6进行了扩大培养,评价菌M7-6的原位代谢活性及与其他微生物类群的竞争作用。结果表明:以甘油为碳源、硝酸盐为氮源、C/N为14.4∶1时,最利于菌株M7-6在模拟油藏条件下生产鼠李糖脂,最小接种量为1%(体积分数)。在厌氧发酵罐中,菌株M7-6可以将培养体系的表面张力降至38.4 mN/m;并且体系中烃降解菌和产酸菌数量有所增加,而硫酸盐还原菌数逐渐减少。
Based on a rhamnolipid producing bacterial strain Pseudomonas aeruginosa M7-6,medium( carbon source,nitrogen source,C/N ratio,and mineral factors) was optimized for efficiently producing rhamnolipid under simulated oil reservoir conditions( temperature,p H and absence of oxygen). Using the optimized medium,effect of different inoculums amount on anaerobic production of rhamnolipid by M7-6was studied. Strain M7-6 was cultured in a 6-L anaerobic fermentor using oilfield production water to prepare medium,to evaluate the rhamnolipid production by M7-6 under simulated conditions and study the competitive activity of M7-6 with other native microorganisms. M7-6 could efficiently produce rhamnolipid under simulated conditions with glycerol as carbon source and nitrate as nitrogen source. Theoptimum C/N ratio is 14. 4 ∶ 1,and the least inoculums amount is 1%( V/V). Strain M7-6 can reduce surface tension of culture system to 38. 4 m N/m in the anaerobic fermenter. Moreover,the numbers of oil degrading bacteria and acid producing bacteria were increased,and the number of sulfate reducing bacteria was decreased.
引文
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