水牛发情周期生殖激素变化规律及唾液结晶的分析
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摘要
试验对水牛发情周期血清和唾液中雌二醇(E_2)和孕酮(P_4)的浓度变化规律、水牛唾液结晶与卵泡发育变化分别进行了分析研究,为进一步探讨水牛发情规律、指导生产提供依据。采用酶联免疫分析法(ELISA)测定发情母水牛血清和唾液中E_2和P_4的浓度变化,并对血清和唾液的激素变化规律进行相关性分析。结果表明,水牛血清和唾液中的E_2和P_4呈波动性变化。发情前期,唾液中P_4浓度一直维持在6.50～7.10 ng/mL,发情第13天达到11.09 ng/mL,随后快速下降。唾液中E_2浓度在发情第3～5天出现一个峰值178.53 pg/mL,在第14～17天唾液中E_2浓度显著升高,出现第二个峰值179.10 pg/mL。母水牛唾液中E_2和P_4浓度的变化趋势与其在血清中的变化趋势基本一致,均呈显著相关(P<0.05);唾液中E_2与P_4浓度呈极显著相关(P<0.01)。水牛发情当天唾液结晶呈现明显的蕨类作物形状且分维值显著低于其他时间点(P<0.05)。水牛发情周期唾液结晶图形的变化与卵巢卵泡发育基本同步,可作为监测水牛发情及预测排卵的可靠指标之一。
The aim of the experiment was to explore the buffalo estrus and provide theoretical references for buffalo production by studying the change laws of estradiol(E_2) and progesterone(P_4) concentrations during estrus cycle of salivary and serum,investigating the salivary ferning and the development of ovaries in buffalo.The concentrations of E_2 and P_4 were determined by the enzyme linked immunosorbent assay(ELISA) in estrus cycle,and the correlation between the serum and saliva hormones were analyzed.The results show that the concentrations of E_2 and P_4 varied fluctuantly.The concentration of saliva P_4 was maintained from 6.50 to 7.10 ng/mL at the beginning of estrus,and reached 11.09 ng/mL at 13 d of estrus,then fell fast.The concentration of saliva E_2 had two peaks,respectively at 3-5 d(178.53 pg/mL) and 14-17 d(179.10 pg/mL) of estrus.The change trends of E_2 and P_4 levels in salivary and serum had the same change trends,the concentrations of salivary P_4 and E_2 had significant correlations with that in serum(P<0.05),while the salivary E_2 concentration also had a extremely significant correlation with the salivary P_4 concentration(P<0.01).Saliva showed a typical fern-like crystallization patterns at 0 d of estrus,with significantly lower fractal dimension values compared with the other days of estrus(P<0.05).The results were also showed the basic synchronization between saliva crystallization and ovarian follicle.In conclusion,saliva crystallization could be used as a reliable index for monitoring estrus and predicting ovulation in buffalo.
The aim of the experiment was to explore the buffalo estrus and provide theoretical references for buffalo production by studying the change laws of estradiol(E_2) and progesterone(P_4) concentrations during estrus cycle of salivary and serum,investigating the salivary ferning and the development of ovaries in buffalo.The concentrations of E_2 and P_4 were determined by the enzyme linked immunosorbent assay(ELISA) in estrus cycle,and the correlation between the serum and saliva hormones were analyzed.The results show that the concentrations of E_2 and P_4 varied fluctuantly.The concentration of saliva P_4 was maintained from 6.50 to 7.10 ng/mL at the beginning of estrus,and reached 11.09 ng/mL at 13 d of estrus,then fell fast.The concentration of saliva E_2 had two peaks,respectively at 3-5 d(178.53 pg/mL) and 14-17 d(179.10 pg/mL) of estrus.The change trends of E_2 and P_4 levels in salivary and serum had the same change trends,the concentrations of salivary P_4 and E_2 had significant correlations with that in serum(P<0.05),while the salivary E_2 concentration also had a extremely significant correlation with the salivary P_4 concentration(P<0.01).Saliva showed a typical fern-like crystallization patterns at 0 d of estrus,with significantly lower fractal dimension values compared with the other days of estrus(P<0.05).The results were also showed the basic synchronization between saliva crystallization and ovarian follicle.In conclusion,saliva crystallization could be used as a reliable index for monitoring estrus and predicting ovulation in buffalo.
引文
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[14] SKALOVA I,FEDOROVA T,BRANDLOVA K.Saliva crystallization in cattle:New possibility for early pregnancy diagnosis?[J].Agricultural Tropica et Subtropica,2013,46(3):102-104.
[15] HABEROVA T.A preliminary study of saliva crystallization inbactrian camels (Camelus bactrianus)[A].4th Scientific Conference of Institute of Tropics and Subtropics:Sustainable Use of Natural Resources in Tropics and Subtropics[C].2010.
[16] CHANDRA S S,PUROHIT G N.Repeat breeding:Incidence,risk factors and diagnosis in buffaloes[J].Asian Pacific Journal of Reproduction,2016,5(2):87-95.
[17] TOM F,CAROLINE D,SARA S,et al.Salivary estradiol as a surrogate marker for serum estradiol in assisted reproduction treatment[J].Clinical Biochemistry,2016,50(3):145-149.
[18] THOZHUKAT S,AHMED A Q,ERIC S,et al.Salivary and serum androgens with anti-Müllerian hormone measurement for the diagnosis of polycystic ovary syndrome[J].Scientific Reports,2018,8(1):3795-3800.
[19] NADIA E F,ALED REES D,CAROL E.Measuring cortisol in serum,urine and saliva are our assays good enough[J].Annals of Clinical Biochemistry,2017,54(3):308-322.
[20] WARRIACH H M,MCGILL D M,BUSH R D,et al.A review of recent developments in buffalo reproduction——A review[J].Asian Australa Animal Science,2015,28:451-455.
[21] SHOKO K,ELEANOR B,AMANDA G,et al.Salivary concentration of progesterone and cortisol significantly differs across individuals after correcting for blood hormone values[J].American Journal of Physical Anthropology,2012,149 (2):231-234.
[22] SABAH P,TAICHI I,IAIN C,et al.The saliva proteome of dogs:Variations within and between breeds and between species[J].Animal Proteomics,2018,18(3-4):1-7.
[2] KOSUKE I G A,NAOKI T,MANABU S,et al.Possibility of diagnosing uterine function in cows[J].Japan Agricultural Research Quarterly,2016,50(2):115-119.
[3] DASH S,CHAKRAVARTY A K,SINGH A.Effect of heat stress on reproductive performances of dairy cattle and buffaloes:A review[J].Veterinary World,2016,9(3):235-244.
[4] ANKITA D V,MANJIT P,NASEER A B.Differential expression of tencandidategenes regulating prostaglandin action in reproductive tissues of buffalo during estrous cycle and pregnancy[J].Theriogenology,2017,105(4):7-14.
[5] AMY R L,ANTHONY C,HACKNEY.Relationship between salivary and serum testosterone levels in response to different exercise intensities[J].Hormones,2015,14(2):258-265.
[6] AJANARAYANAN S R,ARCHUNAN G.Identification of urinary sex pheromones in female buffaloes and their influence on bull reproductive behavior[J].Research in Veterinary Science,2011,91(2):301-305.
[7] KANDASAMY K,SAMUTHIRAPANDI M,DEVARAJ S,et al.Faecal chemical cues in water buffalo that facilitate estrus detection[J].Animal Reproduction Science,2013,138(3-4):163-167.
[8] 祝晓丽,许慧艳,刘晓华,等.广西沼泽型母水牛发情淡季生殖激素的变化规律研究[J].安徽农业科学,2012,40(5):2721-2723. ZHU X L,XU H Y,LIU X H,et al.Variations of reproductive hormones of female swamp buffalos from Guangxi region in an estrus[J].Journal of Anhui Agricultural Science,2012,40(5):2721-2723.(in Chinese)
[9] 谢炳坤.广西黄牛和水牛发情周期血清生殖激素变化规律的研究[D].南宁:广西大学,2005. XIE B K.Studies on changes of serum concentrations of reproductive hormones during the estrous cycle in cattle and buffaloes in Guangxi[D].Nanning:Guangxi University,2005.(in Chinese)
[10] INDU D,PAWAN S,SURERDER S L,et al.Evaluation of salivary electrolytes during estrous cycle in Murrah buffaloes with reference to estrus detection[J].Veterinary World,2016,9(10):1157-1161.
[11] RAVINDER R,ONNUREDDY K,VIJAY S B,et al.Salivaferning,an unorthodox estrus detetion method in water buffaloes (Bubalus bubalis)[J].Theriogenology,2016,86(5):1147-1155.
[12] ANNA K,TAMARA P.Saliva crystallization occurs in female Bornean orangutans (Pongopygmaeus):Could it be a new option for monitoring of menstrual cycle in captive great apes?[J].Public library of science one,2016,11(7):1-11.
[13] PARDO-CARMONA B,MOVANO M R,FERNANDEZ-PALACINOS R,et al.Saliva crystallisation as a means of determining optimal mating time in bitches[J].Journal of Small Animal Practice,2010,51(8):437-442.
[14] SKALOVA I,FEDOROVA T,BRANDLOVA K.Saliva crystallization in cattle:New possibility for early pregnancy diagnosis?[J].Agricultural Tropica et Subtropica,2013,46(3):102-104.
[15] HABEROVA T.A preliminary study of saliva crystallization inbactrian camels (Camelus bactrianus)[A].4th Scientific Conference of Institute of Tropics and Subtropics:Sustainable Use of Natural Resources in Tropics and Subtropics[C].2010.
[16] CHANDRA S S,PUROHIT G N.Repeat breeding:Incidence,risk factors and diagnosis in buffaloes[J].Asian Pacific Journal of Reproduction,2016,5(2):87-95.
[17] TOM F,CAROLINE D,SARA S,et al.Salivary estradiol as a surrogate marker for serum estradiol in assisted reproduction treatment[J].Clinical Biochemistry,2016,50(3):145-149.
[18] THOZHUKAT S,AHMED A Q,ERIC S,et al.Salivary and serum androgens with anti-Müllerian hormone measurement for the diagnosis of polycystic ovary syndrome[J].Scientific Reports,2018,8(1):3795-3800.
[19] NADIA E F,ALED REES D,CAROL E.Measuring cortisol in serum,urine and saliva are our assays good enough[J].Annals of Clinical Biochemistry,2017,54(3):308-322.
[20] WARRIACH H M,MCGILL D M,BUSH R D,et al.A review of recent developments in buffalo reproduction——A review[J].Asian Australa Animal Science,2015,28:451-455.
[21] SHOKO K,ELEANOR B,AMANDA G,et al.Salivary concentration of progesterone and cortisol significantly differs across individuals after correcting for blood hormone values[J].American Journal of Physical Anthropology,2012,149 (2):231-234.
[22] SABAH P,TAICHI I,IAIN C,et al.The saliva proteome of dogs:Variations within and between breeds and between species[J].Animal Proteomics,2018,18(3-4):1-7.