刺五加内生真菌诱导子对宿主生长及抗氧化酶系统影响
|
摘要
该实验旨在研究刺五加内生真菌诱导子对刺五加生长发育及抗氧化酶系统的影响,探索促刺五加生长的诱导子组分。设置5个诱导子组分,分别是孢子诱导子组分(BZ)、整体灭活诱导子组分(MH)、多肽诱导子组分(DT)、菌丝诱导子组分(JS)和小分子诱导子组分(XFZ),收获时测定产量、生长指标、MDA含量及CAT,SOD,POD,ACP,APX酶活性。结果表明,不同组分诱导子和同一组分诱导子不同剂量促生长效果均有差异性,低剂量DT诱导子可显著提高刺五加生物量,降低植株细胞中MDA含量,增强了细胞抗氧化能力。结合产量、生长指标和抗氧化活性,低剂量DT诱导子组分促刺五加生长最佳。
The present study was conducted to explore the effect of endophytic fungi fraction on growth and anti-oxidative activity of Eleutherococcus senticosus. The growth,yield,contents of MDA,and antioxidant activities were assessed in E. senticosus under five fungi fractions,namely BZ,MH,DT,JS,and XFZ. The results showed that fungi fractions and component significantly affected the growth,low concentration of DT fungi fraction significantly increased the biomass of E. senticosus,reduced the MDA content in cells,and the antioxidant activities of the aqueous extracts were superior to the others. The results indicated that low concentration of DT fungi fraction was the optimum fraction to achieve high yield and quality of E. senticosus.
The present study was conducted to explore the effect of endophytic fungi fraction on growth and anti-oxidative activity of Eleutherococcus senticosus. The growth,yield,contents of MDA,and antioxidant activities were assessed in E. senticosus under five fungi fractions,namely BZ,MH,DT,JS,and XFZ. The results showed that fungi fractions and component significantly affected the growth,low concentration of DT fungi fraction significantly increased the biomass of E. senticosus,reduced the MDA content in cells,and the antioxidant activities of the aqueous extracts were superior to the others. The results indicated that low concentration of DT fungi fraction was the optimum fraction to achieve high yield and quality of E. senticosus.
引文
[1]曾建飞.中国植物志.第54卷[M].北京:科学出版社,1978:99.
[2]中国药典.一部[S].2015:206.
[3]中国药典.一部[S].1977:336.
[4] Li T,Kelsey F,Yan Z Q. Acanthopanax senticosus:photo-chemistry and anticancer potential[J]. Am J Chin Med,2016,44:1543.
[5]黄晓巍,刘玥欣,张啸环.刺五加叶化学成分及药理作用研究[J].吉林中医药,2017,37(1):75.
[6]樊如强.刺五加总苷片的制备及其质量研究[D].扬州:扬州大学,2014.
[7]康毅华,王宗权.野生与栽培刺五加叶的质量评价[J].黑龙江医药,2005(6):400.
[8]李阳.木本山野菜种子休眠解除和扦插快繁的研究[D].哈尔滨:东北农业大学,2017.
[9] Da S R A,Polonio J C,Costa A T,et al. Bioprospection of culturable endophytic fungi associated with the ornamental plant Pachystachys lutea[J]. Curr Microbiol,2018,75(5):588.
[10] Bilal L,Asaf S,Hamayun M,et al. Plant growth promoting endophytic fungi Asprgillus fumigatus,TS1 and Fusarium proliferatum,BRL1 produce gibberellins and regulates plant endogenous hormones[J]. Symbiosis,2018,17:1.
[11] Xiang L,Gong S,Yang L,et al. Biocontrol potential of endophytic fungi in medicinal plants from Wuhan Botanical Garden in China[J]. Biol Control,2016,94:47.
[12]刘军,刘艳明,徐在超,等.檀香内生真菌多样性及其抗菌与促生特性的研究[J].中国中药杂志,2018,43(17):3477.
[13]谭燕,贾茹,陶金华,等.内生真菌诱导子调控药用植物活性成分的生物合成[J].中草药,2013,44(14):2004.
[14]夏伟,熊玉萍,朱畇昊,等.真菌诱导子对怀地黄组培苗生长及次生代谢产物合成的影响[J].中国实验方剂学杂志,2017,23(3):25.
[15]张亚庆.棘孢木霉菌溶磷特性及其对植物抗盐性的影响[D].济南:山东师范大学,2015.
[16]王东旭.多肽-氨基酸液态肥料对作物生长和产量的影响[D].北京:中国农业科学院,2011.
[17] Sarathchandra S U,Ghani A,Yeates G W,et al. Effect of nitrogen and phosphate fertilisers on microbial and nematode diversity in pasture soils[J]. Soil Biol Biochem,2001,33(7):953.
[18] Wuest S B. Earthworm,infiltration,and tillage relationships in a dryland pea-wheat rotation[J]. Appl Soil Ecol,2001,18(2):187.
[19] Ming Q,Su C,Zheng C,et al. Elicitors from the endophytic fungus Trichoderma atroviride promote Salvia miltiorrhiza hairy root growth and tanshinone biosynthesis[J]. J Exp Bot,2013,64(18):5687.
[2]中国药典.一部[S].2015:206.
[3]中国药典.一部[S].1977:336.
[4] Li T,Kelsey F,Yan Z Q. Acanthopanax senticosus:photo-chemistry and anticancer potential[J]. Am J Chin Med,2016,44:1543.
[5]黄晓巍,刘玥欣,张啸环.刺五加叶化学成分及药理作用研究[J].吉林中医药,2017,37(1):75.
[6]樊如强.刺五加总苷片的制备及其质量研究[D].扬州:扬州大学,2014.
[7]康毅华,王宗权.野生与栽培刺五加叶的质量评价[J].黑龙江医药,2005(6):400.
[8]李阳.木本山野菜种子休眠解除和扦插快繁的研究[D].哈尔滨:东北农业大学,2017.
[9] Da S R A,Polonio J C,Costa A T,et al. Bioprospection of culturable endophytic fungi associated with the ornamental plant Pachystachys lutea[J]. Curr Microbiol,2018,75(5):588.
[10] Bilal L,Asaf S,Hamayun M,et al. Plant growth promoting endophytic fungi Asprgillus fumigatus,TS1 and Fusarium proliferatum,BRL1 produce gibberellins and regulates plant endogenous hormones[J]. Symbiosis,2018,17:1.
[11] Xiang L,Gong S,Yang L,et al. Biocontrol potential of endophytic fungi in medicinal plants from Wuhan Botanical Garden in China[J]. Biol Control,2016,94:47.
[12]刘军,刘艳明,徐在超,等.檀香内生真菌多样性及其抗菌与促生特性的研究[J].中国中药杂志,2018,43(17):3477.
[13]谭燕,贾茹,陶金华,等.内生真菌诱导子调控药用植物活性成分的生物合成[J].中草药,2013,44(14):2004.
[14]夏伟,熊玉萍,朱畇昊,等.真菌诱导子对怀地黄组培苗生长及次生代谢产物合成的影响[J].中国实验方剂学杂志,2017,23(3):25.
[15]张亚庆.棘孢木霉菌溶磷特性及其对植物抗盐性的影响[D].济南:山东师范大学,2015.
[16]王东旭.多肽-氨基酸液态肥料对作物生长和产量的影响[D].北京:中国农业科学院,2011.
[17] Sarathchandra S U,Ghani A,Yeates G W,et al. Effect of nitrogen and phosphate fertilisers on microbial and nematode diversity in pasture soils[J]. Soil Biol Biochem,2001,33(7):953.
[18] Wuest S B. Earthworm,infiltration,and tillage relationships in a dryland pea-wheat rotation[J]. Appl Soil Ecol,2001,18(2):187.
[19] Ming Q,Su C,Zheng C,et al. Elicitors from the endophytic fungus Trichoderma atroviride promote Salvia miltiorrhiza hairy root growth and tanshinone biosynthesis[J]. J Exp Bot,2013,64(18):5687.