Smac与Survivin在胃溃疡组织中的表达
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摘要
目的研究促凋亡因子(Smac)与生存素(Survivin)在胃溃疡组织中的表达情况。方法选择内江市第一人民医院收治的胃溃疡患者80例,其中无癌前病变胃溃疡40例(N组),癌前病变胃溃疡40例(Y组),使用实时荧光定量聚合酶链反应(RT-PCR)法对两组患者标本中的Smac mRNA和Survivin mRNA进行检测,免疫组化(SP)法对标本中的Smac与Survivin表达进行检测。胃溃疡患者经三联用药治疗,采用脱氧核苷酸末端转移酶介导的dUTP缺口末端标记(TUNEL技术)检测治疗前后细胞凋亡指数。结果 Smac在N组中的阳性(++)与强阳性(+++)的表达占比82.5%,显著高于Y组,占比为47.5%;Survivin在N组中(++)与(+++)的表达占比15.0%,显著低于Y组,占比为35.0%;Smac mRNA在N组中的相对表达量显著高于Y组,而Survivin mRNA在N组中的表达则显著低于Y组;N组经三联用药治疗后的细胞凋亡指数相比治疗前显著降低,差异有统计学意义(P<0.05)。结论临床可将Smac的降低与Survivin的升高作为判定胃溃疡患者组织发生癌前病变的辅助诊断指标,无癌前病变胃溃疡患者经三联用药治疗,能有效控制患者的细胞凋亡指数,提高患者的生存率。
Objective To investigate the expression of pro-apoptotic factor(Smac)and Survivin in gastric ulcer tissue.Methods Selected the 80 cases of gastric ulcer patients as the research object in the first people′s hospital of neijiang,in which no precancerous lesions of 40 cases of gastric ulcer(N group),40 cases of precancerous lesions of gastric ulcer(Y group),two groups of patients were Smac mRNA and Survivin mRNA were detected by using PCR method,immunohistochemical SP method of Smac and Survivin in specimens of table detect.Gastric ulcer patients were treated by triple therapy,and the apoptosis index was detected by TUNEL.Results Smac in N group(++)and(+++)in the expression accounted for 82.5% was higher than that in Y group accounted for 47.5%,Survivin in group N(+ +)and(+ + +)in the expression accounted for 15.0% was significantly lower than Y group accounted for 35.0%;And Smac mRNA in the N group relative expression the amount was significantly higher than that of Y group,while the expression of Survivin mRNA in the N group were significantly lower than Y group;N group the apoptosis index of the triple therapy after treatment than before treatment significantly decreased,the difference was statistically significant(P<0.05).Conclusion The clinical application of Smac and Survivin can be used as an auxiliary diagnostic index for the diagnosis of precancerous lesions in patients with gastric ulcer.Patients with gastric ulcer without precancerous lesion treated by triple therapy which can effectively control the apoptosis index of patients,improve the survival rate of patients.
Objective To investigate the expression of pro-apoptotic factor(Smac)and Survivin in gastric ulcer tissue.Methods Selected the 80 cases of gastric ulcer patients as the research object in the first people′s hospital of neijiang,in which no precancerous lesions of 40 cases of gastric ulcer(N group),40 cases of precancerous lesions of gastric ulcer(Y group),two groups of patients were Smac mRNA and Survivin mRNA were detected by using PCR method,immunohistochemical SP method of Smac and Survivin in specimens of table detect.Gastric ulcer patients were treated by triple therapy,and the apoptosis index was detected by TUNEL.Results Smac in N group(++)and(+++)in the expression accounted for 82.5% was higher than that in Y group accounted for 47.5%,Survivin in group N(+ +)and(+ + +)in the expression accounted for 15.0% was significantly lower than Y group accounted for 35.0%;And Smac mRNA in the N group relative expression the amount was significantly higher than that of Y group,while the expression of Survivin mRNA in the N group were significantly lower than Y group;N group the apoptosis index of the triple therapy after treatment than before treatment significantly decreased,the difference was statistically significant(P<0.05).Conclusion The clinical application of Smac and Survivin can be used as an auxiliary diagnostic index for the diagnosis of precancerous lesions in patients with gastric ulcer.Patients with gastric ulcer without precancerous lesion treated by triple therapy which can effectively control the apoptosis index of patients,improve the survival rate of patients.
引文
[1]陈雷,尚培中.Xiap、Smac与消化系肿瘤的研究进展[J].中国现代普通外科进展,2016,19(4):300-303.
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[12]韩国达,白希永,陆志良,等.凋亡抑制蛋白Survivin在胃癌中的表达及与预后的关系[J].中国现代医学杂志,2015,25(8):29-33.
[13]殷舞,莫祥兰,韦海明,等.Survivin、Apaf-1和caspase-9在胃癌腹膜转移中的表达及意义[J].广东医学,2014,35(2):221-223.
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[15]邢春瑶,冷维春,周家文.Bcl-2和Survivin在宫颈癌中的研究进展[J].中国实验诊断学,2014,18(1):167-169.
[16]叶闻远,欧阳学农,余宗阳.非小细胞肺癌XIAP和Smac的表达与临床病理特征及预后的关系[J].中国肿瘤临床,2014,9(7):444-448.
[17]曲贝贝,左金华.凋亡抑制基因Survivin的研究进展[J].中华临床医师杂志:电子版,2014,9(15):2842-2846.
[2]杨颖,刘国敏,闫国华.促凋亡因子与凋亡抑制蛋白在胃溃疡组织中的表达[J].胃肠病学和肝病学杂志,2014,23(7):759-761.
[3]胡金甫,汪辉,李科珍.Survivin、Bcl-2、HPV16/18在宫颈癌中的表达及相关性研究[J].中国妇幼保健,2014,29(5):781-784.
[4]朱文嘉,李诗敏,王纾宜.Survivin和增殖细胞核抗原(PCNA)在鼻腔鼻窦恶性黑色素瘤(SNM)中的表达及其临床意义[J].复旦学报:医学版,2014,41(5):596-601.
[5]王志红,鲍德明,石振旺,等.溃疡型胃癌和癌前病变中PTEN、Survivin的表达及临床意义[J].临床与实验病理学杂志,2015,24(10):1153-1156.
[6]李艳东,雷海梅,周永莉,等.胃腺癌中survivin和EGFR的作用及其相互关系[J].山西医科大学学报,2014,45(9):839-842.
[7]赵东菊,石太新,王艳蕊,等.儿童急性白血病促凋亡蛋白Smac、HtrA2表达的意义[J].中华实用儿科临床杂志,2015,30(3):207-210.
[8]张小玲,田志逢,王萍,等.KLF5和Survivin蛋白异常表达与胃癌预后的关系[J].临床与实验病理学杂志,2016,32(2):142-145.
[9]许天祥,胡江,刘彦恒.Survivin在胃癌中的表达及其临床意义[J].内蒙古医学杂志,2014,46(10):1153-1156.
[10]郭云霞,王郁杰,王瑞勤,等.胃癌病变过程中Krfippel样因子8及生存蛋白的表达与幽门螺杆菌感染关系[J].中华医院感染学杂志,2015,29(7):1462-1464.
[11]田忠成,邵飞飞,党雪菲,等.Survivin在甲状腺癌中的表达及临床意义[J].中国实验诊断学,2014,18(3):390-393.
[12]韩国达,白希永,陆志良,等.凋亡抑制蛋白Survivin在胃癌中的表达及与预后的关系[J].中国现代医学杂志,2015,25(8):29-33.
[13]殷舞,莫祥兰,韦海明,等.Survivin、Apaf-1和caspase-9在胃癌腹膜转移中的表达及意义[J].广东医学,2014,35(2):221-223.
[14]李冬斌,许香梅,张伟,等.胃癌组织Survivin、RHAMM表达及意义[J].山东医药,2016,56(28):53-54.
[15]邢春瑶,冷维春,周家文.Bcl-2和Survivin在宫颈癌中的研究进展[J].中国实验诊断学,2014,18(1):167-169.
[16]叶闻远,欧阳学农,余宗阳.非小细胞肺癌XIAP和Smac的表达与临床病理特征及预后的关系[J].中国肿瘤临床,2014,9(7):444-448.
[17]曲贝贝,左金华.凋亡抑制基因Survivin的研究进展[J].中华临床医师杂志:电子版,2014,9(15):2842-2846.