盘状结构域受体2在前列腺癌组织标本中的表达及临床意义
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摘要
目的探讨盘状结构域受体2(DDR2)蛋白在前列腺癌组织标本中的表达,分析其与其临床病理特征的关系,初步明确DDR2在前列腺中的作用,为探寻新的治疗靶点提供基础。方法采用免疫组化检测46例前列腺癌和18例良性前列腺增生组织标本中DDR2的表达情况。结果前列腺癌组织的DDR2阳性表达率(82.61%)明显高于良性前列腺增生组织的DDR2的阳性表达率(11.11%,P<0.05)。Gleason 5~7分组和8~10分组前列腺癌组织的DDR2阳性表达率明显高于Gleason 2~4分组前列腺癌组织DDR2阳性表达率(P<0.05)。DDR2蛋白阳性表达与TNM分期比较,TNM分期越晚,DDR2阳性表达率越高(P<0.05)。血清PSA水平≤20μg/L组前列腺癌组织的DDR2阳性表达率明显低于PSA>20μg/L组(P<0.05)。而DDR2阳性表达率与患者的年龄因素无关(P>0.05)。结论 DDR2在前列腺癌中高表达,提示其在前列腺癌的发生发展中可能起重要作用。
Objective To investigate the expression of discoidin domain receptor 2(DDR2)in human prostate cancer and its correlation with the clinicopathologic parameters.Methods Immunohistochemistry assay was performed to detect the expression of DDR2 in 46 cases of prostate cancer and 18 cases of benign prostate hyperplasia.Results The positive expression of DDR2 in prostate cancer(82.61%)was significantly higher than that in benign prostate hyperplasia(11.11%,P<0.05).The positive expression of DDR2 in Gleason 5~7 group and 8~19 group was obviously higher than that in Gleason 2~4 group(P<0.05).The expression of DDR2 was positively related to the TNM stages and increased with tumor progression(P<0.05).The expression of DDR2 was significantly related to serum level of PSA(P<0.05),but not to age.Conclusion The overexpression of DDR2 may play an important role in the pathogenesis of prostate cancer.
Objective To investigate the expression of discoidin domain receptor 2(DDR2)in human prostate cancer and its correlation with the clinicopathologic parameters.Methods Immunohistochemistry assay was performed to detect the expression of DDR2 in 46 cases of prostate cancer and 18 cases of benign prostate hyperplasia.Results The positive expression of DDR2 in prostate cancer(82.61%)was significantly higher than that in benign prostate hyperplasia(11.11%,P<0.05).The positive expression of DDR2 in Gleason 5~7 group and 8~19 group was obviously higher than that in Gleason 2~4 group(P<0.05).The expression of DDR2 was positively related to the TNM stages and increased with tumor progression(P<0.05).The expression of DDR2 was significantly related to serum level of PSA(P<0.05),but not to age.Conclusion The overexpression of DDR2 may play an important role in the pathogenesis of prostate cancer.
引文
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[13]BEAUCHEMIN N.The colorectal tumor microenvironment:the next decade[J].Cancer Microenviron,2011,4(2):181-185.
[14]CHUA HH,YEH TH,WANG YP,et al.Upregulation of discoidin domain receptor 2in nasopharyngeal carcinoma[J].Head Neck,2008,30(4):427-436.
[15]RODRIGUES R,ROQUE L,ESPADINHA C,et al.Comparative genomic hybridization,BRAF,RAS,RET,and oligo-array analysis in aneuploid papillary thyroid carcinomas[J].Oncol Rep,2007,18(4):917-926.
[2]韩苏军,张思维,陈万青,等.中国前列腺癌发病现状和流行趋势分析[J].临床肿瘤学杂志,2013,18(4):330-334.
[3]杜然,郑莉,张惠箴,等.前列腺癌的分子病理学研究进展[J].临床与实验病理学杂志,2011,27(6):632-634.
[4]韩仁强,武鸣,陈万青,等.2003~2007年中国前列腺癌发病与死亡分析[J].中国肿瘤,2012,21(11):805-811.
[5]BORZA CM,POZZI A.Discoidin domain receptors in disease[J].Matrix Biol,2014,34(1):185-192.
[6]CARAFOLI F,HOHENESTER E.Collagen recognition and transmembrane signalling by discoidin domain receptors[J].Biochim Biophys Acta,2013,1834(10):2187-2194.
[7]LEITINGER B.Discoidin domain receptor functions in physiological and pathological conditions[J].Int Rev Cell Mol Biol,2014,310:39-87.
[8]BEAUCHEMIN N.The colorectal tumor microenvironment:the next decade[J].Cancer Microenviron,2011,4(2):181-185.
[9]YASHIMA H,SHIMIZU K,ARAKI T,et al.Assessment of DDR2,BRAF,EGFR and KRAS mutations as therapeutic targets in non-adenocarcinoma lung cancer patients[J].Mol Clin Oncol,2014,2(5):714-718.
[10]REN T,ZHANG W,LIU X,et al.Discoidin domain receptor 2(DDR2)promotes breast cancer cell metastasis and the mechanism implicates epithelial-mesenchymal transition programme under hypoxia[J].J Pathol,2014,234(4):526-537.
[11]BADIOLA I,VILLACP,BASALDUA I,et al.Downregulation of discoidin domain receptor 2in A375human melanoma cells reduces its experimental liver metastasis ability[J].Oncol Rep,2011,26(4):971-978.
[12]LEE NO,PARK JW,LEE JA,et al.Dual action of a selective cyclooxygenase-2inhibitor on vascular endothelial growth factor expression in human hepatocellular carcinoma cells:novel involvement of discoidin domain receptor 2[J].J Cancer Res Clin Oncol,2012,138(1):73-84.
[13]BEAUCHEMIN N.The colorectal tumor microenvironment:the next decade[J].Cancer Microenviron,2011,4(2):181-185.
[14]CHUA HH,YEH TH,WANG YP,et al.Upregulation of discoidin domain receptor 2in nasopharyngeal carcinoma[J].Head Neck,2008,30(4):427-436.
[15]RODRIGUES R,ROQUE L,ESPADINHA C,et al.Comparative genomic hybridization,BRAF,RAS,RET,and oligo-array analysis in aneuploid papillary thyroid carcinomas[J].Oncol Rep,2007,18(4):917-926.