3个地理种源滇黄精光合日变化及其影响因子分析
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摘要
为了解不同种源滇黄精(Polygonatum kingianum)光合生理特性与环境因子的关系,采用LI-6400XT光合作用测定系统,在自然环境条件下测定3个不同地理种源滇黄精植株的光合日变化,为良种选育以及科学栽培提供理论依据。结果表明:3个种源滇黄精的净光合速率(Pn)日变化存在明显差异,其中云南永德种源滇黄精Pn日变化呈单峰曲线,四川会理、云南金平种源滇黄精Pn日变化均呈双峰曲线,金平种源具有典型的"午休"现象。3个种源滇黄精的气孔导度(Gs)和蒸腾速率(Tr)日变化均呈双峰曲线;胞间CO2浓度(Ci)和水分利用率(WUE)日变化均不规则。3个种源之间光合特性存在差异,Pn日均值依次为:云南永德种源>四川会理种源>云南金平种源;除云南永德种源的Tr未出现下降外,其余各光合参数在午后均有较大幅度下降。相关分析表明,云南永德种源的Pn与Gs呈显著正相关;云南金平种源的Pn与Gs、Tr均呈显著正相关;四川会理种源的Pn与Gs、Tr和空气相对湿度(RH)均呈显著正相关,与大气温度(Ta)呈显著负相关。此外,3个种源滇黄精的Gs与RH均呈显著正相关,且各种源的Pn与Gs均呈显著正相关,因此RH对3个种源滇黄精的Pn影响也较大。3个种源滇黄精的光合日进程存在明显差异,说明不同种源对同一环境的适应能力存在较大差别,这种差别在引种栽培时应加以考虑。
In order to understand the relations between photosynthesis parameters and its influencing factors of different geographical populations of Polygonatum kingianum.The photosynthetic day changes of of three different geographical populations of P. kingianum were measured by LI-6400 XT portable photosynthetic system under natural condition,which provides a basis for breeding and cultivation. The results showed that the curves of Pn diurnal variation of the three provenances were different.The daily variation of Pn in provenance of Yongde Yunnan was single peak curve with obvious midday decrease in photosynthesis,but the other two provenances were presented as doublepeak curves. The stomatal conductance(Gs) and transpiration rate(Tr) of the three provenances were presented as double-peak curves,the daily variation of intercellular CO2 concentration(Ci) and water use efficiency(WUE) were irregular.There were differences in photosynthetic characteristics among the three provenances,the rank of Pn averagefrom big to small was Yongde Yunnan,Huili Sichuan and Jinping Yunnan. The photosynthetic parameters showed a large decline except the Tr of Yongde Yunnan in the afternoon.Correlation analysis demonstrated that there was a significant positive correlation between Pn and Gs in provenance of Yongde Yunnan,a significant positive correlation between Pn,Gs and Tr in provenance of Jinping Yunnan,and was a significant positive correlation between Pn, Gs, Tr and air relative humidity(RH) and a significant negative correlation with atmospheric temperature(Ta) in provenance of Huili Sichuan. In addition,there was a significant positive correlation between Gs and RH of three provenances, and Pn and Gs of all provenances showed a significant positive correlation. Therefore, RH was the main influencing factor on Pn of three provenances. There were obvious differences in the photosynthetic daily process of three geographical populations of Polygonatum kingianum. It showed that there was a great difference in the adaptability of different populations to the same environment. This difference would be considered when introducing and cultivating.
In order to understand the relations between photosynthesis parameters and its influencing factors of different geographical populations of Polygonatum kingianum.The photosynthetic day changes of of three different geographical populations of P. kingianum were measured by LI-6400 XT portable photosynthetic system under natural condition,which provides a basis for breeding and cultivation. The results showed that the curves of Pn diurnal variation of the three provenances were different.The daily variation of Pn in provenance of Yongde Yunnan was single peak curve with obvious midday decrease in photosynthesis,but the other two provenances were presented as doublepeak curves. The stomatal conductance(Gs) and transpiration rate(Tr) of the three provenances were presented as double-peak curves,the daily variation of intercellular CO2 concentration(Ci) and water use efficiency(WUE) were irregular.There were differences in photosynthetic characteristics among the three provenances,the rank of Pn averagefrom big to small was Yongde Yunnan,Huili Sichuan and Jinping Yunnan. The photosynthetic parameters showed a large decline except the Tr of Yongde Yunnan in the afternoon.Correlation analysis demonstrated that there was a significant positive correlation between Pn and Gs in provenance of Yongde Yunnan,a significant positive correlation between Pn,Gs and Tr in provenance of Jinping Yunnan,and was a significant positive correlation between Pn, Gs, Tr and air relative humidity(RH) and a significant negative correlation with atmospheric temperature(Ta) in provenance of Huili Sichuan. In addition,there was a significant positive correlation between Gs and RH of three provenances, and Pn and Gs of all provenances showed a significant positive correlation. Therefore, RH was the main influencing factor on Pn of three provenances. There were obvious differences in the photosynthetic daily process of three geographical populations of Polygonatum kingianum. It showed that there was a great difference in the adaptability of different populations to the same environment. This difference would be considered when introducing and cultivating.
引文
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[15]杜晓华,刘会超,袁少寒.三色堇花期光合日变化特性研究[J].河南农业科学,2011,40(4):120-126.
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[18]史开奇,沙吉旦·尕依提,吾马尔·吾哈甫,等.引种杂交杨光合特性及综合评价[J].广东农业科学,2018,45(4):70-79.
[19]刁松锋,邵文豪,董汝湘,等.无患子光合生理日变化及其与生理生态因子的关系[J].西北植物学报,2014,34(4):828-834.
[20]赖晓莲,郭圣茂,丁菲,等.四个种源粉防己夏季光合特性比较[J].北方园艺,2014(20):156-160.
[21]Farquhar G D,Sharkey T D.Stomatal conductance and photosynthesis.Annual Review of Plant Physiology,1982,33(1):317-345.
[22]符云鹏,刘秋员,贺晓辉,等.旺长期不同土壤水分条件下香料烟光合作用的日变化[J].中国烟草学报,2013,19(2):54-60.
[23]姜霞,苏春花,伍生磊,等.白云质砂石山地区翅荚香槐春季光合生理特性研究[J].广东农业科学,2017,44(9):41-47.
[24]王瑞,陈永忠,王湘南,等.油茶优良无性系光合作用的日变化[J].中国农学报,2009,25(24):236-239.
[25]夏尚光,张金池,梁淑英.南方岩榆光合作用日变化及其影响因子研究[J].亚热带植物科学,2007,36(3):8-11,36.
[26]匡经舸,李琬婷,程小毛,等.两种樱花植物的光合速率日变化及其与环境因子的相关性分析[J].北方园艺,2017,41(12):78-82.
[2]柳威,林懋怡,刘晋杰,等.滇黄精研究进展及黄精研究现状[J].中国实验方剂学杂志,2017,23(14):226-234.
[3]年金玉,年贵发,王婷,等.滇黄精的资源分布及仿野生栽培研究[J].农村实用技术,2017(1):22-24.
[4]郝俊杰,韩芸.滇黄精与管花鹿药的药材鉴别研究[J].大理学院学报,2014,13(4):11-13.
[5]周培军,李学芳,符德欢,等.滇黄精与易混品轮叶黄精的比较鉴别[J].广州中医药大学学报,2017,34(4):587-591.
[6]王易芬,穆天慧,陈纪军,等.滇黄精化学成分研究[J].中国中药杂志,2003,28(6):47-50.
[7]陈辉,冯珊珊,孙彦君,等.3种药用黄精的化学成分及药理活性研究进展[J].中草药,2015,46(15):2329-2338.
[8]戴万生,赵声兰,张鹏慧,等.滇黄精3种炮制方法的比较研究[J].西部中医药,2016,29(8):32-34.
[9]郭未艳,孙秋燕,徐晓红,等.滇黄精多糖提取的工艺组合及其优化[J].时珍国医国药,2013,24(6):1391-1393.
[10]陈兴荣,陈玲,马志敏.滇黄精的药用价值与开发利用[J].医药导报,2003,22(4):261-263.
[11]党康,梁宗锁,张跃进.黄精属3种植物的光合特性研究[J].安徽农业科学,2006,34(11):2441-2443.
[12]陈芳软.不同生境条件下多花黄精生长特征及光合特性研究[D].福州:福建农林大学,2013.
[13]骆绪美,郭婉琳,韩文妍.安徽3种黄精植物光合生理生态特性分析[J].安徽农业大学学报,2012,39(5):821-824.
[14]袁名安,蒙世岛,吴梅,等.多花黄精的光合特性日变化规律探析[J].园艺与种苗,2014(12):5-8.
[15]杜晓华,刘会超,袁少寒.三色堇花期光合日变化特性研究[J].河南农业科学,2011,40(4):120-126.
[16]Eamus D.The interaction of rising CO2 and temperatures with water use efficiency[J].Plant,Cell and Environment,1991,14(8):843-852.
[17]夏尚光,肖正东,丁增发,等.油茶优良无性系光合日进程及其与环境因子关系的研究[J].江西农业大学学报,2011,33(5):911-917.
[18]史开奇,沙吉旦·尕依提,吾马尔·吾哈甫,等.引种杂交杨光合特性及综合评价[J].广东农业科学,2018,45(4):70-79.
[19]刁松锋,邵文豪,董汝湘,等.无患子光合生理日变化及其与生理生态因子的关系[J].西北植物学报,2014,34(4):828-834.
[20]赖晓莲,郭圣茂,丁菲,等.四个种源粉防己夏季光合特性比较[J].北方园艺,2014(20):156-160.
[21]Farquhar G D,Sharkey T D.Stomatal conductance and photosynthesis.Annual Review of Plant Physiology,1982,33(1):317-345.
[22]符云鹏,刘秋员,贺晓辉,等.旺长期不同土壤水分条件下香料烟光合作用的日变化[J].中国烟草学报,2013,19(2):54-60.
[23]姜霞,苏春花,伍生磊,等.白云质砂石山地区翅荚香槐春季光合生理特性研究[J].广东农业科学,2017,44(9):41-47.
[24]王瑞,陈永忠,王湘南,等.油茶优良无性系光合作用的日变化[J].中国农学报,2009,25(24):236-239.
[25]夏尚光,张金池,梁淑英.南方岩榆光合作用日变化及其影响因子研究[J].亚热带植物科学,2007,36(3):8-11,36.
[26]匡经舸,李琬婷,程小毛,等.两种樱花植物的光合速率日变化及其与环境因子的相关性分析[J].北方园艺,2017,41(12):78-82.