宁夏枸杞水提物对四氧嘧啶诱导糖尿病小鼠的降糖作用
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摘要
枸杞子被证明具有延缓衰老、抗疲劳、抗脂肪肝以及提高免疫力的功效。作者采用四氧嘧啶建立糖尿病小鼠模型研究宁夏枸杞子水提物(LBWE)的降血糖活性,实验中采用盐酸二甲双胍溶液(Met)作为阳性对照。结果证实,与糖尿病模型组相比较,连续28 d灌胃LBWE,小鼠血糖水平显著降低,四氧嘧啶引起的小鼠体重减轻以及摄食、饮水异常症状得到显著改善。LBWE可以抑制葡萄糖耐量试验中血糖水平的升高,进一步证实在四氧嘧啶诱导的糖尿病小鼠中,宁夏枸杞子水提物具有显著的抗糖尿病的效果。此外,4周的LBWE口服,能够显著降低血清中MDA水平并提高SOD和GSH-Px活性,证明LBWE有助于防止与氧化压力相关的糖尿病并发症。本研究表明宁夏枸杞子水提物通过防止氧化损伤而起到降血糖作用。
Lycium barbarum L. plays roles in anti-aging,anti-fatigue,anti-fatty and immunity improvement. We used alloxan-induced diabetic mouse model to investigate hypoglycemic activity of Lycium Barbarum L. water extract(LBWE). Metformin hydrochloride solution(Met) was served as positive drug. 28-day LBWE treatment significantly reduced mouse blood glucose compared to model group. LBWE strongly improved alloxan-induced weight loss,abnormal symptoms of food and water intake. The hypoglycemic activity of LBWE was further confirmed by the inhibition of serum glucose in glucose tolerance test. Moreover,LBEW-treated mice showed low MDA and high SOD and GSH-Px activity compared to model group,suggested that LBWE prevented oxidative stress associated with diabetes complications. In conclusion,LBWE has a hypoglycemic effect by preventing oxidative damage.
Lycium barbarum L. plays roles in anti-aging,anti-fatigue,anti-fatty and immunity improvement. We used alloxan-induced diabetic mouse model to investigate hypoglycemic activity of Lycium Barbarum L. water extract(LBWE). Metformin hydrochloride solution(Met) was served as positive drug. 28-day LBWE treatment significantly reduced mouse blood glucose compared to model group. LBWE strongly improved alloxan-induced weight loss,abnormal symptoms of food and water intake. The hypoglycemic activity of LBWE was further confirmed by the inhibition of serum glucose in glucose tolerance test. Moreover,LBEW-treated mice showed low MDA and high SOD and GSH-Px activity compared to model group,suggested that LBWE prevented oxidative stress associated with diabetes complications. In conclusion,LBWE has a hypoglycemic effect by preventing oxidative damage.
引文
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[3]SHEN Ziling,ZHANG neng,YU Huarong.The prevention and mechanism of 1,25-dihydroxyvitamin D3 to type 1 diabetes induced by streptozotocin in KM mice through regulating Bax and Bcl-xL protein expression[J].Journal of Chengdu Medical College,2006,1(2):91-95.(in Chinese)
[4]HAMDEN K,JAOUADI B,ZARAI N,et al.Inhibitory effects of estrogens on digestive enzymes,insulin deficiency,and pancreas toxicity in diabetic rats[J].Journal of Physiology and Biochemistry,2011,67:121-128.
[5]郑梅琴.抗糖尿病药物筛选模型的建立及应用[D].沈阳:沈阳药科大学,2006.
[6]PRABHAKAR P K,DOBLE M.Mechanism of action of natural products used in the treatment of diabetes mellitus[J].Chinese Journal of Integartive Medicine,2011,17(8):563-574.
[7]YANG B K,JUNG Y S,SONG C H.Hypoglycemic effects of Ganoderma applanatum and Collybia confluens exo-polymers in streptozotocin-induced diabetic rats[J].Phytotherapy Research,2007,21:1066-1069.
[8]LIU Yanji,LIU Jinfeng,TIAN Xiaoyan,et al.Polysaccharide of actinidia arguta and activity of blood glucose and lipid of decline[J].Journal of Food Science and Biotechnology,2012,31(1):86-89.(in Chinese)
[9]PARK J H,PARK N S,LEE S M,et al.Effect of dongchunghacho rice on blood glucose level,lipid profile,and antioxidant metabolism in streptozotocin-induced diabetic rats[J].Food Science and Biotechnology,2011,20(4):933-940.
[10]ADEWOLE S,ADENOWO T,NAICKER T,et al.Hypoglycaemic and hypotensive effects of Ficus exasperata vahl.(moraceae)leaf aqueous extract in rats[J].African Journal of Traditional Complementary and Alternative Medicines,2011,8(3):275-283.
[11]WANG B S,LEE C P,CHEN Z T,et al.Comparison of the hepatoprotective activity between cultured Cordyceps militaris and natural Cordyceps sinensis[J].Journal of Functional Foods,2012,4(2):489-495.
[12]YANG Shaobo,ZHANG Qian,WANG Juan.Research advances in natural food resources with hypoglycemic effects and their mechanism[J].Food Research and Development,2012,33(4):211-215.(in Chinese)
[13]WU Qingping,XIAO Chun,YANG Xiaobing,et al.Food(medical)use bacteria polysaccharide material down-regulate blood sugar function and its mechanism[J].Acta Edulis Fungi,2009,16(3):80-86.(in Chinese)