蜜环菌提取物对佐剂性关节炎大鼠的治疗作用
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摘要
本文通过对佐剂性关节炎大鼠原发性和继发性足肿胀度的测量、全身关节炎指数评分、体重及体态变化、免疫脏器指数、炎性踝关节切片的形态以及血清中肿瘤坏死因子α、白细胞介素1β、白细胞介素6、前列腺素E2、一氧化氮和一氧化氮合成酶等指标来考察蜜环菌Armillaria mellea的5个不同提取物对佐剂性关节炎大鼠的影响及作用机理。结果表明,蜜环菌甲醇提取物对原发性和继发性足肿胀有明显的抑制;甲醇组能明显抑制佐剂性关节炎大鼠脾脏和胸腺萎缩,并能抑制血清中肿瘤坏死因子α、白细胞介素1β、白细胞介素6和前列腺素E2的分泌,抑制大鼠踝关节炎细胞的形成。因此蜜环菌提取物中甲醇提取物对佐剂性关节炎大鼠治疗效果最显著。
The primary and secondary foot swelling in adjuvant arthritis rats(AA rats), systemic arthritis index score, body weight and body state changes, immune organ index, inflammatory ankle joint morphology and serum TNF-α, IL-1β, IL-6, PGE2, NO and NOS were used to investigate the effects of five different extracts of Armillaria mellea on adjuvant arthritis rats. The results showed that the high-dose and low-dose experimental groups treated with A. mellea methanol extract significantly manifested inhibiting effects on primary and secondary foot swelling of rats. The methanol extract can significantly inhibit the spleen and thymus atrophy and secretion of TNF-α, IL-1β, IL-6 and PGE2 in serum of AA rats, and also inhibit the formation of rat sputum arthritis cells. Facts have proved that the methanol extract of A. mellea has therapeutic effect on adjuvant arthritis rats.
The primary and secondary foot swelling in adjuvant arthritis rats(AA rats), systemic arthritis index score, body weight and body state changes, immune organ index, inflammatory ankle joint morphology and serum TNF-α, IL-1β, IL-6, PGE2, NO and NOS were used to investigate the effects of five different extracts of Armillaria mellea on adjuvant arthritis rats. The results showed that the high-dose and low-dose experimental groups treated with A. mellea methanol extract significantly manifested inhibiting effects on primary and secondary foot swelling of rats. The methanol extract can significantly inhibit the spleen and thymus atrophy and secretion of TNF-α, IL-1β, IL-6 and PGE2 in serum of AA rats, and also inhibit the formation of rat sputum arthritis cells. Facts have proved that the methanol extract of A. mellea has therapeutic effect on adjuvant arthritis rats.
引文
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Bradley JR,2010.“TNF-mediated inflammatory disease.”Journal of Pathology,214(2):149-160
Chen JS,Yu HR,Zhang YL,Tong JM,2012.Effects of total saponins of radix paeoniae alba on serum cytokines in rats with rheumatoid arthritis.Lishizhen Medicine and Materia Medica Research,23(5):1097-1098(in Chinese)
Dai YC,2012.Research on wood decay fungi in Chinese woody plant pathogens.Mycosystema,31:493-509(in Chinese)
Dai YC,Yang ZL,2008.The list of Chinese medicinal fungi and the revision of some names.Mycosystema,27:801-824(in Chinese)
Dai YC,Zhou LW,Yang ZL,Wen HA,Bau T,Li TH,2010.List of edible fungi in China.Mycosystema,29:1-21(in Chinese)
Koch AE,2007.The pathogenesis of rheumatoid arthritis.American Journal of Orthopedics,36(7):5-12
Komatsu N,Takayanagi H,2012.Autoimmune arthritis:the interface between the immune system and joints.Advances in Immunology,115:45-71
Li CY,Li XH,Lv JM,Gao H,2008.Experimental study on the regulation of immune function of mice by cucumber aroma.Lishizhen Medicine and Materia Medica Research,19(1):40-41(in Chinese)
Lu XH,2015.Observation on the curative effect of turf sputum extract on rheumatoid arthritis.Pharmacology and Clinics of Chinese Materia Medica,31(1):258-260(in Chinese)
LüSM,Meng LS,Zhu WH,2014.Toll-like receptors are involved in the pathogenesis of rheumatoid arthritis.Journal of Xi’an Jiaotong University(Medical Sciences),35(4):423-428(in Chinese)
Mcinnes IB,Buckley CD,Isaacs JD,2015.Cytokines in rheumatoid arthritis-shaping the immunological landscape.Nature Reviews Rheumatology,12:63-68
Qian L,LüLJ,Xu M,Wang XT,2011.Toll-like receptor 4induced by peripheral blood mononuclear cells in patients with activated rheumatoid arthritis induces th17cell differentiation.Chinese Journal of Rheumatology,15(12):816-820(in Chinese)
Rao C,Huang X,2010.Advances in the anti-inflammatory and anti-tumor mechanisms of flavonoids.Strait Pharmaceutical Journal,22(6):8-11(in Chinese)
Rudnicka W,Burakowski T,Warnawin E,Jastrzebska M,Bik M,Kontny E,2010.Functional tlr9 modulates bone marrow b cells from rheumatoid arthritis patients.European Journal of Immunology,39(5):1211-1220
Song SS,Zhang LL,Wei W,2011.Progress in the establishment of animal models of experimental arthritis and pathological mechanisms.Chinese Pharmacological Bulletin,27(12):1648-1653(in Chinese)
Szekanecz Z,Koch AE,2016.Successes and failures of chemokine-pathway targeting in rheumatoid arthritis.Nature Reviews Rheumatology,12(1):5-13
Tian J,Chen JW,Gao JS,Li L,Xie X,2013.Resveratrol inhibits tnf-α-induced iL-1β,mmp-3 production in human rheumatoid arthritis fibroblast-like synoviocytes via modulation of pi3kinase/akt pathway.Rheumatology International,33(7):1829-1835
Uhlig T,Moe RH,Kvien TK,2014.The burden of disease in rheumatoid arthritis.Pharmaco Economics,32(9):841-851
Wang Q,Xu YC,Dong W,2002.Effects of systemic regulation of nitric oxide levels on adjuvant arthritis in rats.Chinese Journal of Rheumatology,6(3):209-210(in Chinese)
Wu H,Huang Q,Qi Z,Chen Y,Wang A,Chen C,2017.Irreversible inhibition of btk kinase by a novel highly selective inhibitor chmfl-btk-11 suppresses inflammatory response in rheumatoid arthritis model.Scientific Reports,7(1):466
Zeng XF,Zhu SL,Tan AC,Xie XP,2013.Systematic evaluation of disease burden and quality of life in rheumatoid arthritis in China.Chinese Journal of Evidence-based Medicine,13(3):300-307(in Chinese)
Zhang L,Bai RY,2008.New progress in the treatment of cytokines and rheumatoid arthritis.China Medical Herald,5(11):27-29(in Chinese)
Zhang MN,2011.Experimental animal model of rheumatoid arthritis and its evaluation.Laboratory Animal Science,28(4):57-59(in Chinese)
Zhu SL,2016.Screening of anti-inflammatory active ingredients of Armillaria mellea and its mechanism of action.Master Thesis,Jiangnan University,Wuxi.8-40(in Chinese)
陈建双,于海荣,张玉玲,佟继铭,2012.赤雹根总皂苷对类风湿性关节炎大鼠血清细胞因子的影响.时珍国医国药,23(5):1097-1098
戴玉成,2012.中国木本植物病原木材腐朽菌研究.菌物学报,31:493-509
戴玉成,杨祝良,2008.中国药用真菌名录及部分名称的修订.菌物学报,27:801-824
戴玉成,周丽伟,杨祝良,文华安,图力古尔,李泰辉,2010.中国食用菌名录.菌物学报,29:1-21
李春艳,李先辉,吕江明,高辉,2008.黄瓜香对小鼠免疫功能调节的实验研究.时珍国医国药,19(1):40-41
卢秀花,2015.草木樨流浸膏片对类风湿性关节炎的疗效观察.中药药理与临床,31(1):258-260
吕社民,孟列素,朱文华,2014.Toll样受体参与类风湿性关节炎发病机制的研究进展.西安交通大学学报(医学版),35(4):423-428
钱雷,吕丽君,徐敏,汪晓莺,2011.活化的类风湿关节炎患者外周血单个核细胞toll样受体4诱导th17细胞分化.中华风湿病学杂志,15(12):816-820
饶澄,黄显,2010.黄酮类化合物抗炎和抗肿瘤共同作用机制的研究进展.海峡药学,22(6):8-11
宋珊珊,张玲玲,魏伟,2011.实验性关节炎动物模型建立及病理机制研究进展.中国药理学通报,27(12):1648-1653
王强,徐玉川,董巍,2002.全身调控一氧化氮水平对大鼠佐剂性关节炎的影响.中华风湿病学杂志,6(3):209-210
曾小峰,朱松林,谭爱春,谢小平,2013.我国类风湿关节炎疾病负担和生存质量研究的系统评价.中国循证医学杂志,13(3):300-307
张良,白人骁,2008.细胞因子与类风湿性关节炎治疗新进展.中国医药导报,5(11):27-29
张敏娜,2011.类风湿关节炎的实验动物模型及其评价.实验动物科学,28(4):57-59
朱水玲,2016.蜜环菌抗炎活性成分筛选及作用机制初探.江南大学硕士论文,无锡.8-40
Bradley JR,2010.“TNF-mediated inflammatory disease.”Journal of Pathology,214(2):149-160
Chen JS,Yu HR,Zhang YL,Tong JM,2012.Effects of total saponins of radix paeoniae alba on serum cytokines in rats with rheumatoid arthritis.Lishizhen Medicine and Materia Medica Research,23(5):1097-1098(in Chinese)
Dai YC,2012.Research on wood decay fungi in Chinese woody plant pathogens.Mycosystema,31:493-509(in Chinese)
Dai YC,Yang ZL,2008.The list of Chinese medicinal fungi and the revision of some names.Mycosystema,27:801-824(in Chinese)
Dai YC,Zhou LW,Yang ZL,Wen HA,Bau T,Li TH,2010.List of edible fungi in China.Mycosystema,29:1-21(in Chinese)
Koch AE,2007.The pathogenesis of rheumatoid arthritis.American Journal of Orthopedics,36(7):5-12
Komatsu N,Takayanagi H,2012.Autoimmune arthritis:the interface between the immune system and joints.Advances in Immunology,115:45-71
Li CY,Li XH,Lv JM,Gao H,2008.Experimental study on the regulation of immune function of mice by cucumber aroma.Lishizhen Medicine and Materia Medica Research,19(1):40-41(in Chinese)
Lu XH,2015.Observation on the curative effect of turf sputum extract on rheumatoid arthritis.Pharmacology and Clinics of Chinese Materia Medica,31(1):258-260(in Chinese)
LüSM,Meng LS,Zhu WH,2014.Toll-like receptors are involved in the pathogenesis of rheumatoid arthritis.Journal of Xi’an Jiaotong University(Medical Sciences),35(4):423-428(in Chinese)
Mcinnes IB,Buckley CD,Isaacs JD,2015.Cytokines in rheumatoid arthritis-shaping the immunological landscape.Nature Reviews Rheumatology,12:63-68
Qian L,LüLJ,Xu M,Wang XT,2011.Toll-like receptor 4induced by peripheral blood mononuclear cells in patients with activated rheumatoid arthritis induces th17cell differentiation.Chinese Journal of Rheumatology,15(12):816-820(in Chinese)
Rao C,Huang X,2010.Advances in the anti-inflammatory and anti-tumor mechanisms of flavonoids.Strait Pharmaceutical Journal,22(6):8-11(in Chinese)
Rudnicka W,Burakowski T,Warnawin E,Jastrzebska M,Bik M,Kontny E,2010.Functional tlr9 modulates bone marrow b cells from rheumatoid arthritis patients.European Journal of Immunology,39(5):1211-1220
Song SS,Zhang LL,Wei W,2011.Progress in the establishment of animal models of experimental arthritis and pathological mechanisms.Chinese Pharmacological Bulletin,27(12):1648-1653(in Chinese)
Szekanecz Z,Koch AE,2016.Successes and failures of chemokine-pathway targeting in rheumatoid arthritis.Nature Reviews Rheumatology,12(1):5-13
Tian J,Chen JW,Gao JS,Li L,Xie X,2013.Resveratrol inhibits tnf-α-induced iL-1β,mmp-3 production in human rheumatoid arthritis fibroblast-like synoviocytes via modulation of pi3kinase/akt pathway.Rheumatology International,33(7):1829-1835
Uhlig T,Moe RH,Kvien TK,2014.The burden of disease in rheumatoid arthritis.Pharmaco Economics,32(9):841-851
Wang Q,Xu YC,Dong W,2002.Effects of systemic regulation of nitric oxide levels on adjuvant arthritis in rats.Chinese Journal of Rheumatology,6(3):209-210(in Chinese)
Wu H,Huang Q,Qi Z,Chen Y,Wang A,Chen C,2017.Irreversible inhibition of btk kinase by a novel highly selective inhibitor chmfl-btk-11 suppresses inflammatory response in rheumatoid arthritis model.Scientific Reports,7(1):466
Zeng XF,Zhu SL,Tan AC,Xie XP,2013.Systematic evaluation of disease burden and quality of life in rheumatoid arthritis in China.Chinese Journal of Evidence-based Medicine,13(3):300-307(in Chinese)
Zhang L,Bai RY,2008.New progress in the treatment of cytokines and rheumatoid arthritis.China Medical Herald,5(11):27-29(in Chinese)
Zhang MN,2011.Experimental animal model of rheumatoid arthritis and its evaluation.Laboratory Animal Science,28(4):57-59(in Chinese)
Zhu SL,2016.Screening of anti-inflammatory active ingredients of Armillaria mellea and its mechanism of action.Master Thesis,Jiangnan University,Wuxi.8-40(in Chinese)
陈建双,于海荣,张玉玲,佟继铭,2012.赤雹根总皂苷对类风湿性关节炎大鼠血清细胞因子的影响.时珍国医国药,23(5):1097-1098
戴玉成,2012.中国木本植物病原木材腐朽菌研究.菌物学报,31:493-509
戴玉成,杨祝良,2008.中国药用真菌名录及部分名称的修订.菌物学报,27:801-824
戴玉成,周丽伟,杨祝良,文华安,图力古尔,李泰辉,2010.中国食用菌名录.菌物学报,29:1-21
李春艳,李先辉,吕江明,高辉,2008.黄瓜香对小鼠免疫功能调节的实验研究.时珍国医国药,19(1):40-41
卢秀花,2015.草木樨流浸膏片对类风湿性关节炎的疗效观察.中药药理与临床,31(1):258-260
吕社民,孟列素,朱文华,2014.Toll样受体参与类风湿性关节炎发病机制的研究进展.西安交通大学学报(医学版),35(4):423-428
钱雷,吕丽君,徐敏,汪晓莺,2011.活化的类风湿关节炎患者外周血单个核细胞toll样受体4诱导th17细胞分化.中华风湿病学杂志,15(12):816-820
饶澄,黄显,2010.黄酮类化合物抗炎和抗肿瘤共同作用机制的研究进展.海峡药学,22(6):8-11
宋珊珊,张玲玲,魏伟,2011.实验性关节炎动物模型建立及病理机制研究进展.中国药理学通报,27(12):1648-1653
王强,徐玉川,董巍,2002.全身调控一氧化氮水平对大鼠佐剂性关节炎的影响.中华风湿病学杂志,6(3):209-210
曾小峰,朱松林,谭爱春,谢小平,2013.我国类风湿关节炎疾病负担和生存质量研究的系统评价.中国循证医学杂志,13(3):300-307
张良,白人骁,2008.细胞因子与类风湿性关节炎治疗新进展.中国医药导报,5(11):27-29
张敏娜,2011.类风湿关节炎的实验动物模型及其评价.实验动物科学,28(4):57-59
朱水玲,2016.蜜环菌抗炎活性成分筛选及作用机制初探.江南大学硕士论文,无锡.8-40