非洲猪瘟检测技术进展
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摘要
非洲猪瘟(African swine fever,ASF)是由非洲猪瘟病毒(African swine fever virus,ASFV)引起的一种急性、烈性、高病死率的传染病。ASFV能感染所有品种及各个年龄段的猪,目前暂无针对ASF的疫苗。在流行ASF的国家,防止其传播只能依靠准确的诊断和严格的检疫措施。因此,快速可靠的检测方法对于及时诊断和预防该病是至关重要的。本文旨在归纳总结非洲猪瘟检测方法和技术手段,为在不同现场情况下都能快速排查非洲猪瘟提供更多选择。
African swine fever(ASF)is an infectious disease caused by the African swine fever virus(ASFV)with high fatality rate that affects pigs of all breeds and ages. Currently,there is no vaccine against ASF. In countries where ASF is prevalent,the only way to prevent its spread is to rely on accurate diagnostic procedures and quarantine measures. Therefore,rapid and reliable detection of the ASFV is of great importance for timely implementation of control measures to stop ASF spread. Here,we summarize the Office International des Epi-zooties(OIE)test for ASF and common diagnostic testing methods to provide a theoretical basis for rapid clini-cal screening for ASF.
African swine fever(ASF)is an infectious disease caused by the African swine fever virus(ASFV)with high fatality rate that affects pigs of all breeds and ages. Currently,there is no vaccine against ASF. In countries where ASF is prevalent,the only way to prevent its spread is to rely on accurate diagnostic procedures and quarantine measures. Therefore,rapid and reliable detection of the ASFV is of great importance for timely implementation of control measures to stop ASF spread. Here,we summarize the Office International des Epi-zooties(OIE)test for ASF and common diagnostic testing methods to provide a theoretical basis for rapid clini-cal screening for ASF.
引文
[1]World Organization for Animal Health(OIE).African swine fever.Chapter 2.8.1.In:Manual of Diagnostic Tests and Vaccines for Terrestrial Animals[M].Paris,France:OIE,2012.
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[5]Mckillen J,Mcmenamy M,Hjertner B,McNeilly F,Uttenthal?,Gallardo C,Adair B,Allan G.Sensitive detection of African swine fever virus using real-time PCR with a 5′conjugated minor groove binder probe[J].J Virol Methods,2010,168(1):141-146.
[6]Tignon M,Gallardo C,Iscaro C,Hutet E,der Stede YV,Kolbasov D,De Mia G M,Le Potier M F,Bishop R P,Arias M,Koenen F.Development and inter-labora-tory validation study of an improved new real-time PCRassay with internal control for detection and laboratory di-agnosis of African swine fever virus[J].J Virol Meth-ods,2011,178(1-2):161-170.
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[8]Grau F R,Schroeder M E,Mulhern E L,McIntosh MT,Bounpheng M A.Detection of African swine fever,classical swine fever,and foot-and-mouth disease viruses in swine oral fluids by multiplex reverse transcription real-time polymerase chain reaction[J].J Vet Diagn Invest,2015,27(2):140-149.
[9]Petrov A,Beer M,Blome S.Development and valida-tion of a harmonized TaqMan-based triplex real-time RT-PCR protocol for the quantitative detection of normalized gene expression profiles of seven porcine cytokines[J/OL].PLoS One,2014,9(9):e108910.
[10]Luo Y,Atim S A,Shao L,Ayebazibwe C,Sun Y,Liu Y,Ji S,Meng X Y,Li S,Li Y,Masembe C,St?hl K,Widén F,Liu L,Qiu H J.Development of an updated PCR assay for detection of African swine fever virus[J].Arch Virol,2017,162(1):191-199.
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[12]Hu L,Lin X Y,Yang Z X,Yao X P,Li G L,Peng ZS,Wang Y.A multiplex PCR for simultaneous detec-tion of classical swine fever virus,African swine fever vi-rus,highly pathogenic porcine reproductive and respira-tory syndrome virus,porcine reproductive and respirato-ry syndrome virus and pseudorabies in swines[J].Pol JVet Sci,2015,18(4):715-723.
[13]冷依伊,任梅渗,蒙正群,张鹏飞,杨泽晓,姚学萍,王印.5种猪病毒性传染病病原的多重PCR检测方法的建立[J].中国预防兽医学报,2017,39(2):123-126.
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[17]Xu G,Hu L,Zhong H,Wang H,Yusa S,Weiss T C,Romaniuk P J,Pickerill S,You Q.Cross Priming Am-plification:Mechanism and optimization for isothermal DNA amplification[J].Sci Rep,2012,2:246.
[18]交叉引物扩增-试纸条法:一种快速现地检测非洲猪瘟病毒的新技术[J/OL].中国预防兽医学报,2018(11):1086[2019-01-12].http://kns.cnki.net/kcms/detail/23.1417.s.20181203.0936.044.html.
[19]Fr?czyk M,Wo?niakowski G,Kowalczyk A,Niemczuk K,Pejsak Z.Development of cross priming-amplifica-tion for direct detection of the African Swine Fever Vi-rus,in pig and wild boar blood and sera samples[J].Lett Appl Microbiol,2016,62(5):386-391.
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[22]郭正洋,刘钟栋,刘小青,陈晶,兰全学.重组酶聚合酶扩增技术的研究进展[J].食品科技,2018,43(9):55-59.
[23]高威芳,朱鹏,黄海龙.重组酶聚合酶扩增技术:一种新的核酸扩增策略[J].中国生物化学与分子生物学报,2016,32(6):627-634.
[24]Wang J,Wang J,Geng Y,Yuan W.A recombinase polymerase amplification-based assay for rapid detection of African swine fever virus[J].Can J Vet Res,2017,81(4):308-312.
[25]Wo?niakowski G,Fr?czyk M,Kowalczyk A,Pomorska-Mól M,Niemczuk K,Pejsak Z.Polymerase cross-link-ing spiral reaction(PCLSR)for detection of African swine fever virus(ASFV)in pigs and wild boars[J].Sci Rep,2017,7(1):42903.
[26]Larsson G L,Helenius G.Digital droplet PCR(ddP-CR)for the detection and quantification of HPV 16,18,33 and 45-a short report[J].Cell Oncol,2017,40(Suppl 5):1-7.
[27]OlmedillasLópez S,GarcíaArranz M,GarcíaOlmo D.Current and emerging applications of droplet digital PCRin oncology[J].Mol Diagn Ther,2017,21(5):493-510.
[28]邬旭龙,肖璐,宋勇,林华,陈世界,杨苗,安微,姚学萍,杨泽晓,王印.非洲猪瘟病毒微滴数字PCR(ddP-CR)方法的建立及应用[J].微生物学通报,2017,44(12):2839-2846.
[29]Hu L,Lin X,Nie F,Yang Z,Yao X,Li G,Wu X,Ren M,Wang Y.Simultaneous typing of seven porcine pathogens by multiplex PCR with a GeXP analyser[J].J Virol Methods,2016,232:21-28.
[30]Song Q,Yang H,Zou B,Kajiyama T,Kambara H,Zhou G.Improvement of LATE-PCR to allow single-cell analysis by pyrosequencing[J].Analyst,2013,138:4991-4997.
[31]Lyu J,Yu Y,Pan C,Zhou J,Ren X.Development and validation of a method for human papillomavirus genotyp-ing based on molecular beacon probes[J/OL].PLoSOne,2018,13(11):e0207930.
[32]Ronish B,Hakhverdyan M,St?hl K,Belák S,LeBlanc N,Wangh L.Design and verification of a highly reliable Linear-After-The-Exponential PCR(LATE-PCR)as-say for the detection of African swine fever virus[J].JVirol Methods,2011,172(1):8-15.
[33]Biagetti M,Cuccioloni M,Bonfili L,Cecarini V,Se-bastiani C,Curcio L,Giammarioli M,De Mia G M,Eleuteri A M,Angeletti M.Chimeric DNA/LNA-based biosensor for the rapid detection of African swine fever virus[J].Talanta,2018,184:35-41.
[34]Fernández-Pinero J,Gallardo C,Elizalde M,Robles A,Gómez C,Bishop R,Heath L,Couacy-Hymann E,Fasina F Q,Pelayo V,Soler A,Arias M.Molecular di-agnosis of African Swine Fever by a new real-time PCRusing universal probe library[J].Transbound Emerg Dis,2013,60(1):48-58.
[35]Vidal M I,Stiene M,Henkel J,Bilitewski U,Costa JV,Oliva A G.A solid-phase enzyme linked immunosor-bent assay using monoclonal antibodies,for the detection of African swine fever virus antigens and antibodies[J].J Virol Methods,1997,66(2):211-218.
[36]Sastre P,Gallardo C,Monedero A,Ruiz T,Arias M,Sanz A,Rueda P.Development of a novel lateral flow assay for detection of African swine fever in blood[J].BMC Vet Res,2016,12(1):206.
[37]Sastre P,Pérez T,Costa S,Yang X,R?ber A,Blome S,Goller K V,Gallardo C,Tapia I,García J,Sanz A,Rueda P.Development of a duplex lateral flow assay for simultaneous detection of antibodies against African and Classical swine fever viruses[J].J Vet Diagn Invest,2016,28(5):543-549.
[38]Mur L,Gallardo C,Soler A,Zimmermman J,Pelayo V,Nieto R,Sánchez-Vizcaíno J M,Arias M.Potential use of oral fluid samples for serological diagnosis of Afri-can swine fever[J].Vet Microbiol,2013,165(1-2):135-139.
[39]Gallardo C,Reis A L,Kalema-Zikusoka G,Malta J,Soler A,Blanco E,Parkhouse R M E,Leit?o A.Re-combinant antigen targets for serodiagnosis of African Swine Fever[J].Clin Vaccine Immunol,2009,16(7):1012-1020.
[40]Bergeron H C,Glas P S,Schumann K R.Diagnostic specificity of the African swine fever virus antibody de-tection enzyme-linked immunosorbent assay in feral and domestic pigs in the United States[J].Transbound Emerg Dis,2017,64(1).
[41]邬旭龙,彭彬,姜睿姣,肖璐,王印,姚学萍,杨泽晓,张鹏飞,张博.以原核表达的非洲猪瘟病毒pK205R蛋白为包被抗原的间接ELISA抗体检测方法的建立[J].中国预防兽医学报,2016,38(10):800-803.
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[2]王涛,孙元,罗玉子,仇华吉.非洲猪瘟防控及疫苗研发:挑战与对策[J].生物工程学报,2018,34(12):1931-1942.
[3]包雨鑫,丁军莉,郑海英,吴佳琦.非洲猪瘟研究进展[J].黑龙江畜牧兽医,2018(24):78-80.
[4]King D P,Reid S M,Hutchings G H,Grierson S S,Wilkinson P J,Dixon L K,Bastos Armanda D S,Drew T W.Development of a TaqMan?PCR assay with inter-nal amplification control for the detection of African swine fever virus[J].J Virol Methods,2003,107(1):53-61.
[5]Mckillen J,Mcmenamy M,Hjertner B,McNeilly F,Uttenthal?,Gallardo C,Adair B,Allan G.Sensitive detection of African swine fever virus using real-time PCR with a 5′conjugated minor groove binder probe[J].J Virol Methods,2010,168(1):141-146.
[6]Tignon M,Gallardo C,Iscaro C,Hutet E,der Stede YV,Kolbasov D,De Mia G M,Le Potier M F,Bishop R P,Arias M,Koenen F.Development and inter-labora-tory validation study of an improved new real-time PCRassay with internal control for detection and laboratory di-agnosis of African swine fever virus[J].J Virol Meth-ods,2011,178(1-2):161-170.
[7]Haines F J,Hofmann M A,King D P,Drew T W,Crooke H R.Development and validation of a multiplex,real-time RT PCR assay for the simultaneous detection of classical and African swine fever viruses[J/OL].PLoS One,2013,8(7):e71019.
[8]Grau F R,Schroeder M E,Mulhern E L,McIntosh MT,Bounpheng M A.Detection of African swine fever,classical swine fever,and foot-and-mouth disease viruses in swine oral fluids by multiplex reverse transcription real-time polymerase chain reaction[J].J Vet Diagn Invest,2015,27(2):140-149.
[9]Petrov A,Beer M,Blome S.Development and valida-tion of a harmonized TaqMan-based triplex real-time RT-PCR protocol for the quantitative detection of normalized gene expression profiles of seven porcine cytokines[J/OL].PLoS One,2014,9(9):e108910.
[10]Luo Y,Atim S A,Shao L,Ayebazibwe C,Sun Y,Liu Y,Ji S,Meng X Y,Li S,Li Y,Masembe C,St?hl K,Widén F,Liu L,Qiu H J.Development of an updated PCR assay for detection of African swine fever virus[J].Arch Virol,2017,162(1):191-199.
[11]任梅渗,冷依伊,蒙正群,张鹏飞,杨泽晓,姚学萍,王印.7种猪呼吸道综合征疾病病原多重PCR方法的建立[J].中国农业大学学报,2017,22(7):47-53.
[12]Hu L,Lin X Y,Yang Z X,Yao X P,Li G L,Peng ZS,Wang Y.A multiplex PCR for simultaneous detec-tion of classical swine fever virus,African swine fever vi-rus,highly pathogenic porcine reproductive and respira-tory syndrome virus,porcine reproductive and respirato-ry syndrome virus and pseudorabies in swines[J].Pol JVet Sci,2015,18(4):715-723.
[13]冷依伊,任梅渗,蒙正群,张鹏飞,杨泽晓,姚学萍,王印.5种猪病毒性传染病病原的多重PCR检测方法的建立[J].中国预防兽医学报,2017,39(2):123-126.
[14]Zhang X,Lowe S B,Gooding J J.Brief review of moni-toring methods for loop-mediated isothermal amplifica-tion(LAMP)[J].Biosens Bioelectron,2014,61:491-499.
[15]James H E,Ebert K,Mcgonigle R,Reid S M,Boon-ham N,Tomlinson J A,Hutchings G H,Denyer M,Oura C,Dukes J P,King D P.Detection of African swine fever virus by loop-mediated isothermal amplifica-tion[J].J Virol Methods,2010,164(1-2):68-74.
[16]邬旭龙.快速检测非洲猪瘟病毒环介导等温扩增方法的建立[D].四川农业大学,2014.
[17]Xu G,Hu L,Zhong H,Wang H,Yusa S,Weiss T C,Romaniuk P J,Pickerill S,You Q.Cross Priming Am-plification:Mechanism and optimization for isothermal DNA amplification[J].Sci Rep,2012,2:246.
[18]交叉引物扩增-试纸条法:一种快速现地检测非洲猪瘟病毒的新技术[J/OL].中国预防兽医学报,2018(11):1086[2019-01-12].http://kns.cnki.net/kcms/detail/23.1417.s.20181203.0936.044.html.
[19]Fr?czyk M,Wo?niakowski G,Kowalczyk A,Niemczuk K,Pejsak Z.Development of cross priming-amplifica-tion for direct detection of the African Swine Fever Vi-rus,in pig and wild boar blood and sera samples[J].Lett Appl Microbiol,2016,62(5):386-391.
[20]Li J,Macdonald J,Stetten F V.Review:a comprehen-sive summary of a decade development of the recombi-nase polymerase amplification[J].Analyst,2018,144(1):31-67.
[21]Daher R K,Stewart G,Boissinot M,Bergeron M G.Recombinase polymerase amplification for diagnostic ap-plications[J].Clin Chem,2016,62(7):947-958.
[22]郭正洋,刘钟栋,刘小青,陈晶,兰全学.重组酶聚合酶扩增技术的研究进展[J].食品科技,2018,43(9):55-59.
[23]高威芳,朱鹏,黄海龙.重组酶聚合酶扩增技术:一种新的核酸扩增策略[J].中国生物化学与分子生物学报,2016,32(6):627-634.
[24]Wang J,Wang J,Geng Y,Yuan W.A recombinase polymerase amplification-based assay for rapid detection of African swine fever virus[J].Can J Vet Res,2017,81(4):308-312.
[25]Wo?niakowski G,Fr?czyk M,Kowalczyk A,Pomorska-Mól M,Niemczuk K,Pejsak Z.Polymerase cross-link-ing spiral reaction(PCLSR)for detection of African swine fever virus(ASFV)in pigs and wild boars[J].Sci Rep,2017,7(1):42903.
[26]Larsson G L,Helenius G.Digital droplet PCR(ddP-CR)for the detection and quantification of HPV 16,18,33 and 45-a short report[J].Cell Oncol,2017,40(Suppl 5):1-7.
[27]OlmedillasLópez S,GarcíaArranz M,GarcíaOlmo D.Current and emerging applications of droplet digital PCRin oncology[J].Mol Diagn Ther,2017,21(5):493-510.
[28]邬旭龙,肖璐,宋勇,林华,陈世界,杨苗,安微,姚学萍,杨泽晓,王印.非洲猪瘟病毒微滴数字PCR(ddP-CR)方法的建立及应用[J].微生物学通报,2017,44(12):2839-2846.
[29]Hu L,Lin X,Nie F,Yang Z,Yao X,Li G,Wu X,Ren M,Wang Y.Simultaneous typing of seven porcine pathogens by multiplex PCR with a GeXP analyser[J].J Virol Methods,2016,232:21-28.
[30]Song Q,Yang H,Zou B,Kajiyama T,Kambara H,Zhou G.Improvement of LATE-PCR to allow single-cell analysis by pyrosequencing[J].Analyst,2013,138:4991-4997.
[31]Lyu J,Yu Y,Pan C,Zhou J,Ren X.Development and validation of a method for human papillomavirus genotyp-ing based on molecular beacon probes[J/OL].PLoSOne,2018,13(11):e0207930.
[32]Ronish B,Hakhverdyan M,St?hl K,Belák S,LeBlanc N,Wangh L.Design and verification of a highly reliable Linear-After-The-Exponential PCR(LATE-PCR)as-say for the detection of African swine fever virus[J].JVirol Methods,2011,172(1):8-15.
[33]Biagetti M,Cuccioloni M,Bonfili L,Cecarini V,Se-bastiani C,Curcio L,Giammarioli M,De Mia G M,Eleuteri A M,Angeletti M.Chimeric DNA/LNA-based biosensor for the rapid detection of African swine fever virus[J].Talanta,2018,184:35-41.
[34]Fernández-Pinero J,Gallardo C,Elizalde M,Robles A,Gómez C,Bishop R,Heath L,Couacy-Hymann E,Fasina F Q,Pelayo V,Soler A,Arias M.Molecular di-agnosis of African Swine Fever by a new real-time PCRusing universal probe library[J].Transbound Emerg Dis,2013,60(1):48-58.
[35]Vidal M I,Stiene M,Henkel J,Bilitewski U,Costa JV,Oliva A G.A solid-phase enzyme linked immunosor-bent assay using monoclonal antibodies,for the detection of African swine fever virus antigens and antibodies[J].J Virol Methods,1997,66(2):211-218.
[36]Sastre P,Gallardo C,Monedero A,Ruiz T,Arias M,Sanz A,Rueda P.Development of a novel lateral flow assay for detection of African swine fever in blood[J].BMC Vet Res,2016,12(1):206.
[37]Sastre P,Pérez T,Costa S,Yang X,R?ber A,Blome S,Goller K V,Gallardo C,Tapia I,García J,Sanz A,Rueda P.Development of a duplex lateral flow assay for simultaneous detection of antibodies against African and Classical swine fever viruses[J].J Vet Diagn Invest,2016,28(5):543-549.
[38]Mur L,Gallardo C,Soler A,Zimmermman J,Pelayo V,Nieto R,Sánchez-Vizcaíno J M,Arias M.Potential use of oral fluid samples for serological diagnosis of Afri-can swine fever[J].Vet Microbiol,2013,165(1-2):135-139.
[39]Gallardo C,Reis A L,Kalema-Zikusoka G,Malta J,Soler A,Blanco E,Parkhouse R M E,Leit?o A.Re-combinant antigen targets for serodiagnosis of African Swine Fever[J].Clin Vaccine Immunol,2009,16(7):1012-1020.
[40]Bergeron H C,Glas P S,Schumann K R.Diagnostic specificity of the African swine fever virus antibody de-tection enzyme-linked immunosorbent assay in feral and domestic pigs in the United States[J].Transbound Emerg Dis,2017,64(1).
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