碳氮比对活性污泥胞外聚合物的长期影响
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摘要
以人工模拟废水为研究对象,采用4组SBR反应器(R0,R5,R10和R15),重点考察了碳氮比(C/N)对胞外聚合物(EPS)含量及其组分(蛋白质(PN)、多糖(PS)和核酸(DNA))的影响.试验结果表明:C/N对EPS及其组分具有显著影响.随着C/N由0升高至15,EPS和紧密结合型胞外聚合物(TB-EPS)含量逐渐升高,而松散型胞外聚合物(LB-EPS)含量逐渐降低,EPS以TB-EPS为主(占77.4%~93.6%).EPS和TB-EPS中的PN、PS和DNA含量随着C/N值升高而升高,LB-EPS中的PN、PS和DNA含量随C/N升高而降低.此外,随着C/N的增大,毛细吸水时间(CST)和污泥比阻(SRF)值显著增大,污泥的脱水性能变差.
In this paper,the long-term effect of C/N ratio on extracellular polymeric substance(EPS) in the sequencing batch reactor(SBR) treating synthetic wastewater was investigated under four C/N ratio conditions(0,5,10,15).The results showed that the C/N ratio had a significant impact on EPS and its composition.With the increase of C/N ratio(0→5→10→15),EPS and tightly bound EPS(TB-EPS) content increased,while,loosely bound EPS(LB-EPS) decreased slowly.TB-EPS was dominant in EPS(the percentage of TB-EPS/EPS was 77.4~93.6%).Moreover,protein(PN),Carbohydrate(PS) and DNA in EPS and TB-EPS increased with the C/N ratio increasing.PN,PS and DNA in LB-EPS decreased as the C/N ratio increased.Moreover,capillary suction times(CST) and specific resistance to filtration(SRF) increased when the C/N ratio increased,and caused deterioration in sludge dewaterability.
In this paper,the long-term effect of C/N ratio on extracellular polymeric substance(EPS) in the sequencing batch reactor(SBR) treating synthetic wastewater was investigated under four C/N ratio conditions(0,5,10,15).The results showed that the C/N ratio had a significant impact on EPS and its composition.With the increase of C/N ratio(0→5→10→15),EPS and tightly bound EPS(TB-EPS) content increased,while,loosely bound EPS(LB-EPS) decreased slowly.TB-EPS was dominant in EPS(the percentage of TB-EPS/EPS was 77.4~93.6%).Moreover,protein(PN),Carbohydrate(PS) and DNA in EPS and TB-EPS increased with the C/N ratio increasing.PN,PS and DNA in LB-EPS decreased as the C/N ratio increased.Moreover,capillary suction times(CST) and specific resistance to filtration(SRF) increased when the C/N ratio increased,and caused deterioration in sludge dewaterability.
引文
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[27]Cetin S,Erdincler A.The role of carbohydrate and protein parts of extracellular polymeric substances on the dewaterability of biological sludges[J].Water Science&Technology A Journal of the International Association on Water Pollution Research,2004,50(9):49–56.
[28]Jahn A,Nielsen P H.Cell biomass and exopolymer composition in sewer biofilms[J].Water Science and Technology,1998,37(1):17-24.
[29]王红武,李晓岩,赵庆祥.胞外聚合物对活性污泥沉降和絮凝性能的影响研究[J].中国安全科学学报,2003,13(9):31-34.
[30]何培培,余光辉,邵立明,等.污泥中蛋白质和多糖的分布对脱水性能的影响[J].环境科学,2008,29(12):3457-3461.
[31]Higgins M J,Novak J T.The effect of cations on the settling and dewatering of activated sludges[J].Water Environment Research,1997,69(2):215-224.
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[2]Zhang X,Bishop P L,Kupferle M J.Measurement of polysaccharides and proteins in biofilm extracellular polymers[J].Water Science&Technology,2015,37(4/5):345-348.
[3]Liu Y Q,Liu Y,Tay J H.The effects of extracellular polymeric substances on the formation and stability of biogranules[J].Applied Microbiology&Biotechnology,2004,65(2):143-148.
[4]Poxon T L,Darby J L.Extracellular polyanions in digested sludge:measurement and relationship to sludge dewaterability[J].Water Research,1997,31(4):749-758.
[5]Ye F X,Ye Y F,Li Y.Effect of C/N ratio on extracellular polymeric substances(EPS)and physicochemical properties of activated sludge flocs[J].Journal of Hazardous Materials,2011,188(1-3):37-43.
[6]Wang Z,Gao M,Xin Y,et al.Effect of C/N ratio on extracellular polymeric substances of activated sludge from an anoxic-aerobic sequencing batch reactor treating saline wastewater[J].Environmental Technology,2014,35(24):2821-2828.
[7]Miqueleto A P,Dolosic C C,Pozzi E,et al.Influence of carbon sources and C/N ratio on EPS production in anaerobic sequencing batch biofilm reactors for wastewater treatment[J].Bioresource Technology,2010,101(4):1324-1330.
[8]吴志高.胞外聚合物(EPS)对污泥沉降性能的影响及其在生物除磷中的作用研究[D].重庆:重庆大学,2006.
[9]夏志红.碳氮比对硝化污泥胞外聚合物污泥特性的影响研究[D].西安:西安建筑科技大学,2014.
[10]Magara Y,Numbu S,Utosawa K.Biochemical and physical properties of an activated sludge on settling Characteristics[J].Water Research,1986,10(1):71-77.
[11]Ye F X,Peng G,Li Y.Influences of influent carbon source on extracellular polymeric substances(EPS)and physicochemical properties of activated sludge[J].Chemosphere,2011,84(9):1250-1255.
[12]国家环境保护局.水和废水监测分析方法[M].北京:中国环境科学出版社,2002:200-281.
[13]Mengistu Y,Edwards C,Saunders J R.Continuous culture studies on the synthesis of capsular polysaccharide by Klebsiella pneumoniae K1[J].Journal of Applied Bacteriology,2010,76(5):424-430.
[14]Neijssel O M,Tempest D W.The regulation of carbohydrate metabolism in Klebsiella aerogenes NCTC 418organisms,growing in chemostat culture[J].Archives of Microbiology,1975,106(3):251-258.
[15]Linton J D,Jones D S,Woodward S.Factors that control the rate of exopolysaccharide production by agrobacterium radiobacter NCIB 11883[J].Microbiology,1987,133(11):2979-2987.
[16]Yang S F,Li X Y.Influences of extracellular polymeric substances(EPS)on the characteristics of activated sludge under nonsteady-state conditions[J].Process Biochem,2009,44(1),91-96.
[17]Liu H,Fang H H P.Extraction of extracellularpolymeric substances(EPS)sludges[J].Journal of Biotechnology,2002,95(3):249-256.
[18]Liu H,Fang H H P.Influences of extracellularpolymeric subtances(EPS)on flocculation,settling,anddewatering of activated sludge[J].Critical Reviews in Environmental Science and Technology,2003,33(3):237-273.
[19]Fr?lund B,Palmgren R,Keiding K,et al.Extraction of extracellular polymers from activated sludge using a cation exchange resin[J].Water Research,1996,30(8):1749-1758.
[20]Morgan J W,Forster C F,Evison L.A comparative study of the nature of biopolymers extracted from anaerobic and activated sludges[J].Water Research,1990,24(6):743-750.
[21]Chen W,Westerhoff P,Leenheer J A,et al.Fluorescence excitation-Emission matrix regional integration to quantify spectra for dissolved organic matter[J].environmental science&technology,2003,37(24):5701-5710.
[22]Sanin F D,Vatansever A,Turtin I,et al.Operational conditions of activated sludge:influence on flocculation and dewaterability[J].Drying Technol,2006,24(10):1297-1306.
[23]Lee C H,Liu J C.Enhanced sludge dewatering by dual polyectrolytes conditioning[J].Water Research,2000,34(18):4430–4436.
[24]Li X Y,Yang S F.Influence of loosely bound extracellular polymeric substances(EPS)on the flocculation,sedimentation and dewaterability of activated sludge[J].Water Research,2007,41(5):1022-1030.
[25]Eriksson L,Alm B.Study of flocculation mechanism by observing effects of a complexing agent on activated sludge properties[J].Waterence&Technology,1991,24(7):21-28.
[26]Sponza D T.Investigation of extracellular polymer substances(EPS)and physicochemical properties of different activated sludge flocs under steady-state conditions[J].Enzyme and Microbial Technology,2003,32(3):375-385.
[27]Cetin S,Erdincler A.The role of carbohydrate and protein parts of extracellular polymeric substances on the dewaterability of biological sludges[J].Water Science&Technology A Journal of the International Association on Water Pollution Research,2004,50(9):49–56.
[28]Jahn A,Nielsen P H.Cell biomass and exopolymer composition in sewer biofilms[J].Water Science and Technology,1998,37(1):17-24.
[29]王红武,李晓岩,赵庆祥.胞外聚合物对活性污泥沉降和絮凝性能的影响研究[J].中国安全科学学报,2003,13(9):31-34.
[30]何培培,余光辉,邵立明,等.污泥中蛋白质和多糖的分布对脱水性能的影响[J].环境科学,2008,29(12):3457-3461.
[31]Higgins M J,Novak J T.The effect of cations on the settling and dewatering of activated sludges[J].Water Environment Research,1997,69(2):215-224.
[32]Forstor C F.Factors involved in the settlement of activated sludge-I:nutrients and surface polymers[J].Water Research,1985,19(10):1259-1264.