白介素-6及血清肿瘤坏死因子-α诊断尖锐湿疣的检验分析
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摘要
目的探讨血清肿瘤坏死因子-α(TNF-α)及白介素-6(IL-6)诊断尖锐湿疣的价值。方法选取2015年6月至2017年3月鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)诊治的81例尖锐湿疣患者作为研究对象,将这81例患者设为观察组。另选取2015年6月至2017年3月鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)接待的56例健康体检者作为对照组。再将观察组81例患者中的尖锐湿疣初发患者30例设为初发组,尖锐湿疣复发患者51例设为复发组。入组研究对象均于清晨空腹抽取3 mL外周静脉血,分离血清,采用酶联免疫吸附法(ELISA法)测定血清TNF-α和IL-6含量。比较观察组和对照组组间血清TNF-α和IL-6含量变化,观察组组内所分的初发组和复发组血清TNF-α和IL-6含量变化,及TNF-α+IL-6诊断方法学效能。结果观察组血清TNF-α和IL-6含量低于对照组,且差异具有统计学意义(P<0.05);初发组血清TNF-α和IL-6含量高于对照组,且差异具有统计学意义(P<0.05);TNF-α+IL-6诊断特异度和灵敏度高于TNF-α和IL-6单项检测。结论尖锐湿疣患者血清TNF-α和IL-6含量均降低,TNF-α+IL-6诊断特异度和灵敏度较高,具有重要临床研究价值。
Objective To investigate the value of serum tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in the diagnosis of condylomaacuminatum(CA). Methods 81 patients with CA and 56 healthy people for physical examination in Huangshi Central Hospital of Eastern Hubei Medical Group from June 2015 to March 2017 were selected as the observation group and control group respectively. Of the 81 patients in the observation group, 30 ones with initial CA were regarded as the initial group and the rest 51 ones with recurrent CA as the recurrent group. 3 mL fasting peripheral venous blood was taken and the levels of serum TNF-α and IL-6 were measured by enzyme-linked immunosorbent assay(ELISA). The changes in serum TNF-α and IL-6 levels were compared between the observation group and the control group, as well as between the initial group and the recurrent groups. The diagnostic efficacy of TNF-α and IL-6 was also compared. Results The levels of serum TNF-α and IL-6 in the observation group were lower than those in control group, with statistically significant differences(P< 0 05). The levels of serum TNF-α and IL-6 in the initial group were significantly higher than those in the control group, with statistically significant differences(P< 0 05). The specificity and sensitivity of TNF-α joint with IL-6 was higher than those of TNF-α and IL-6 alone. Conclusions The serum levels of TNF-α and IL-6 in patients with CA are lower with high diagnostic specificity and sensitivity, which is of important clinical value
Objective To investigate the value of serum tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in the diagnosis of condylomaacuminatum(CA). Methods 81 patients with CA and 56 healthy people for physical examination in Huangshi Central Hospital of Eastern Hubei Medical Group from June 2015 to March 2017 were selected as the observation group and control group respectively. Of the 81 patients in the observation group, 30 ones with initial CA were regarded as the initial group and the rest 51 ones with recurrent CA as the recurrent group. 3 mL fasting peripheral venous blood was taken and the levels of serum TNF-α and IL-6 were measured by enzyme-linked immunosorbent assay(ELISA). The changes in serum TNF-α and IL-6 levels were compared between the observation group and the control group, as well as between the initial group and the recurrent groups. The diagnostic efficacy of TNF-α and IL-6 was also compared. Results The levels of serum TNF-α and IL-6 in the observation group were lower than those in control group, with statistically significant differences(P< 0 05). The levels of serum TNF-α and IL-6 in the initial group were significantly higher than those in the control group, with statistically significant differences(P< 0 05). The specificity and sensitivity of TNF-α joint with IL-6 was higher than those of TNF-α and IL-6 alone. Conclusions The serum levels of TNF-α and IL-6 in patients with CA are lower with high diagnostic specificity and sensitivity, which is of important clinical value
引文
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[2] 杨宪鲁,王海燕,张锐利,等.5-氨基酮戊酸光动力疗法联合胸腺五肽对复发性尖锐湿疣患者外周血白细胞介素-17、白细胞介素-23的影响[J].中国基层医药,2016,23(16):2530-2533.
[3] 郭欣颖,郭爱敏.尖锐湿疣患者生活质量及其影响因素的调查研究[J].中国护理管理,2016,16(4):477-480.
[4] 郑青,梁宁,陈夏明.尖锐湿疣患者治疗前后血清IL-2、IL-6、TNF-α和外周血B细胞、T淋巴细胞亚群检测的临床意义[J].放射免疫学杂志,2007,20(3):238-240.
[5] 牟勖东,任虹,解春桃.尖锐湿疣患者治疗前后血清TNF-α、hs-CRP和GM-CSF检测的临床意义[J].放射免疫学杂志,2009,22(3):210-211.
[6] 中华人民共和国卫生部卫生防疫司.性病防治手册[M].南京:江苏科学技术出版社,1994:57-64.
[7] 王一鸣,程燕,树瑜.尖锐湿疣患者的细胞免疫功能及其与阴茎包皮组织中HPV DNA水平的相关分析[J].山东医药,2015,7(25):87-89.
[8] 雷振春,郑一斐,汪英俊,等.尖锐湿疣患者人乳头状瘤病毒的感染与基因分型研究[J].中华医院感染学杂志,2015(7):1475-1477.
[9] 方军,何弘.尖锐湿疣患者血清IL-8、IL-10、TNF-α和hs-CRP检测的临床意义[J].放射免疫学杂志,2010,23(3):305-306.
[10] 王亚平.尖锐湿疣患者治疗前后血清TNF-α、IL-6、IL-8、IL-10检测的临床意义[J].放射免疫学杂志,2007,20(6):525-526.
[11] 陆玲娜,冯广红,石年.血清肿瘤坏死因子-α和白介素-6的水平检测在尖锐湿疣诊断中的应用研究[J].中国性科学,2015,24(3):61-63.
[12] 覃永健,冷文婷,黄琴.疣毒清合剂对复发性尖锐湿疣患者血清TNF-α、IL-2的影响及疗效观察[J].中药新药与临床药理,2011,15(5):566-568.
[13] 徐小英.新型三阶段疗法对肛周尖锐湿疣患者血清IL-2、IFN-γ、IL-6细胞因子的影响[J].现代诊断与治疗,2016,24(12):2157-2159.
[14] 王希晶,王谦可,梁凤仪,等.ALA-PDT联合CO2激光治疗尖锐湿疣疗效观察及治疗前后外周血TNF-α和IL-6变化[J].中国皮肤性病学杂志,2012,26(3):233-234.
[15] 马静,温炬,郑荣昌,等.低剂量阿维A对尖锐湿疣复发患者血清细胞因子水平的影响[J].广东医学,2013,34(11):1768-1770.
[16] Kofoed K,Norrbom C,Forslund O,et al.Low prevalence of oral and nasal human papillomavirus in employees performing CO2-laser evaporation of genital warts or loop electrode excision procedure of cervical dysplasia[J].Acta dermato-venereologica,2015,95(2):173-176.
[17] Semu M,Fenta TG,Medhin G,et al.Effectiveness of isoniazid preventative therapy in reducing incidence of active tuberculosis among people living with HIV/AIDS in public health facilities of Addis Ababa,Ethiopia:A historical cohort study[J].BMC Infectious Diseases,2017,17(1):1-8.