陆地棉Gh NHX7基因特征及表达的生物信息学分析
摘要
为了挖掘棉花盐胁迫调控基因,解析Gh NHX7基因在棉花基因组中的结构与功能,根据拟南芥盐胁迫调控基因At NHX7/SOS1的蛋白序列,通过同源性搜索,在陆地棉基因组中获得了2个GhNHX7基因。同时,对GhNHX7基因结构、蛋白结构、启动子顺式作用元件及盐胁迫下的表达进行了分析。结果表明,Gh NHX7a和GhNHX7b具有与At NHX7/SOS1相似的基因序列与蛋白结构域和类似的顺式作用元件,可能参与陆地棉盐胁迫响应。
引文
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[4]Ishitani M,Liu J,Halfter U,et al.SOS3 function in plant salt tolerance requires N-myristoylation and calcium binding[J].Plant Cell,2000,12(9):1667-1678.
[5]Guo Y,Qiu Q S,Quintero F J,et al.Transgenic evaluation of activated mutant alleles of SOS2 reveals a critical requirement for its kinase activity and C-terminal regulatory domain for salt tolerance in Arabidopsis thaliana[J].Plant Cell,2004,16(2):435-449.
[6]Guo Y,Halfter U,Ishitani M,et al.Molecular characterization of functional domains in the protein kinase SOS2 that is required for plant salt tolerance[J].Plant Cell,2001,13(6):1383-1400.
[7]杨平,周峰,张边江,等.植物盐超敏感基因(SOS1)的演化分析与选择压力检测[J].分子植物育种,2016,14(8):2098-2105.
[8]Wang K,Wang Z,Li F,et al.The draft genome of a diploid cotton Gossypium raimondii[J].Nature Genetics,2012,44(10):1098-1103.
[9]Li F,Fan G,Wang K,et al.Genome sequence of the cultivated cotton Gossypium arboreum[J].Nature Genetics,2014,3(6):567-572.
[10]Du X,Huang G,He S,et al.Resequencing of 243 diploid cotton accessions based on an updated A genome identifies the genetic basis of key agronomic traits[J].Nature Genetics,2018,50(3):796-802.
[11]Zhang T,Hu Y,Jiang W,et al.Sequencing of allotetraploid cotton(Gossypium hirsutum L.acc.TM-1)provides a resource for fiber improvement[J].Nature Biotechnology,2015,33(5):531-537.
[12]Hu B,Jin J,Guo AY,et al.GSDS 2.0:an upgraded gene feature visualization server[J].Bioinformatics,2015,31(8):1296-1297.
[13]Wang Y,Tang H,Debarry JD,et al.MCScanX:a toolkit for detection and evolutionary analysis of gene synteny and collinearity[J].Nucleic Acids Research,2012,40(7):e49.https://doi.org/10.1093/nar/gkr1293.
[14]Higo K,Ugawa Y,Iwamoto M,et al.Plant cis-acting regulatory DNA elements(PLACE)database:1999[J].Nucleic Acids Research,1999,27(1):297-300.
[15]许艳超,韦洋洋,李振庆,等.复合盐碱胁迫下半野生棉苗期耐盐性综合评价及其关键生理指标的变化[J].棉花学报,2018,30(3):231-241.
[16]Zhu J K.Abiotic stress signaling and responses in plants[J].Cell,2016,167(2):313-324.
[17]Gong Z,Koiwa H,Cushman M A,et al.Genes that are uniquely stress regulated in salt overly sensitive(sos)mutants[J].Plant Physiology,2001,126(1):363-375.
[18]Gong D,Guo Y,Jagendorf A T,et al.Biochemical characterization of the Arabidopsis protein kinase SOS2 that functions in salt tolerance[J].Plant Physiology,2002,130(1):256-264.
[19]Sanchez-Barrena M J,Martinez-Ripoll M,Zhu J K,et al.SOS3(salt overly sensitive 3)from Arabidopsis thaliana:expression,purification,crystallization and preliminary X-ray analysis[J].Acta Crystallographica,2004,60(Pt7):1272-1274.
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