梨火疫病抑菌制剂的室内筛选
|
摘要
【目的】筛选抑制梨火疫菌(Erwinia amylovora)的药剂,为防控梨火疫病提供参考与依据。【方法】采用最低抑制浓度和抑菌圈比较的方法,对30种抑菌制剂进行筛选。【结果】内吸性抑菌制剂1.8%辛菌胺醋酸盐,有机类抑菌制剂3%噻霉酮(ME)、3%噻霉酮(WP)和30%壬菌铜,植物源类型制剂80%乙蒜素以及微生物制剂荧光假单胞杆菌3000,是室内试验对梨火疫菌抑菌效果较好的制剂。【结论】不同药剂之间、同一种药剂不同剂型之间抑菌效果差异较大,研究筛选得到的6种抑菌制剂可为梨火疫的防治提供参考。
【Objective】 Fire blight(Erwinia amylovora) is one of the most devastating bacterial diseases of plant species in the Rosaceae family. Screening of the agents and microorganism preparation against fire blight bacteria can help prevent and control the disease.【Method】Thirty kinds of antibacterial agents and preparations were screened in the laboratory using the methods of minimum inhibitory concentration and bacteriostatic circle comparison.【Result】The results showed that among 30 kinds of agents, 1.8% symplectic bacteria amine acetate, 3% benziothiazolinone(ME) and 3% benziothiazolinone(WP), 30% nonyl bacteria copper, 80% ethylicin, and the microorganism preparation Fluorescence pseudomonas 300 billion spores per gram were the agents and microorganism preparation with better bacteriostatic effects.【Conclusion】 The antibacterial effects of different agents and different dosage forms of the same agent were significantly different. The six antibacterial agents obtained in this study can provide theoretical and practical basis for the quarantine control of fire blight.
【Objective】 Fire blight(Erwinia amylovora) is one of the most devastating bacterial diseases of plant species in the Rosaceae family. Screening of the agents and microorganism preparation against fire blight bacteria can help prevent and control the disease.【Method】Thirty kinds of antibacterial agents and preparations were screened in the laboratory using the methods of minimum inhibitory concentration and bacteriostatic circle comparison.【Result】The results showed that among 30 kinds of agents, 1.8% symplectic bacteria amine acetate, 3% benziothiazolinone(ME) and 3% benziothiazolinone(WP), 30% nonyl bacteria copper, 80% ethylicin, and the microorganism preparation Fluorescence pseudomonas 300 billion spores per gram were the agents and microorganism preparation with better bacteriostatic effects.【Conclusion】 The antibacterial effects of different agents and different dosage forms of the same agent were significantly different. The six antibacterial agents obtained in this study can provide theoretical and practical basis for the quarantine control of fire blight.
引文
[1]刘华威.梨火疫病原菌核糖体基因结构序列的特征研究及病菌的分子检测[D].新疆农业大学硕士论文,2008LIU Hua-wei.(2008).Sequence characteristics of ribosomal genetic structure Erwinia amylovora and Molecular Detetion for the pathogen [D].Master Dissertation,Xinjiang Agricultrual University.Urumqi.(in Chinese)
[2] Zwet,T.V.D.,& Keil,H.L.(1979).Fire blight,a bacterial disease of Rosaceous plants..Fire blight :a bacterial disease of rosaceous plants.U.S.Dept.of Agriculture.
[3] Cabrefiga,J.,& Montesinos,E.(2005).Analysis of aggressiveness of erwinia amylovora using disease-dose and time relationships.Phytopathology,95(12):1,430.
[4]胡白石.梨火疫病菌的风险分析及检测技术研究[J].南京:南京农业大学博士论文,2000,HU Bai-shi.(2000).Study on risk analysis and detection technology of Erwinia amylovora [J].PhD Dissertation.Nanjing Agricultural University,Nanjing.(in Chinese)
[5]胡白石,许志刚,周国梁,等.梨火疫病的进境风险分析[J].植物保护学报,2001,(4):303-308.HUA Bai-shi,XU Zhi-gang,ZHOU Guo-liang,et al.(2001).Risk analysis of pear fire blight in China [J].Journal of Plant Protection,(4):303-308.(in Chinese)
[6] Michele,G.,Urs,K.,Matthias,M.,Monika,M.,& Isabel,H.G.(2015).Fire blight control:the struggle goes on.a comparison of different fire blight control methods in switzerland with respect to biosafety,efficacy and durability.International Journal of Environmental Research and Public Health,12(9):11,422-11,447.
[7] Mcghee,G.C.,Guasco,J.,Bellomo,L.M.,Blumer-Schuette,S.E.,Shane,W.W.,& Irish-Brown,A.,et al.(2011).Genetic analysis of streptomycin-resistant (sm-r) strains of erwinia amylovora suggests that dissemination of two genotypes is responsible for the current distribution of sm-r e.amylovora in michigan.Phytopathology,101(2):182.
[8] Tancos,K.A.,Cox,K.D.Effects of consecutive streptomycin and kasugamycin applications on epiphyticbacteria in the apple phyllosphere.[J].Plant disease.2017,101(1):158-164.
[9] Johnson,K.B.(1993).Effect of antagonistic bacteria on establishment of honey bee-dispersed erwinia amylovora in pear blossoms and on fire blight control.Phytopathology,83(7):995-1,002.
[10] Johnson,K.B.,Stockwell,V.O.,Burgett,D.M.,Sugar,D.,& Loper,J.E.(1993).Dispersal of erwinia amylovora and pseudomonas fluorescens by honey bees from hives to apple and pear blossoms.Phytopathology,83(5):478-484.
[11] Lindow,S.E.,Mcgourty,G.,& Elkins,R.(1996).Interactions of antibiotics with pseudomonas fluorescens strain a506 in the control of fire blight and frost injury to pear.Phytopathology,86(8):841-848.
[12] Lehman S M .Development of a bacteriophage-based biopesticide for fire blight [J].Brock University,2007.
[13] 王钊.安祖花细菌性枯萎病病原菌的分子检测与药剂室内筛选[D].北京:中国农业科学院硕士论文,2013.WANG Zhao .(2013).Detection of Xanthomonas axonopodis pv.dieffenbachiae in Authurium andreanum and Laboratory Screening of bactericides [D].Master Dissertation.Chinese Academy of Agricultural Sciences,Beijing.(in Chinese)
[14] 慕立义.植物化学保护研究方法[M].北京:中国农业出版社,1994:39-45.MU Li -yi.(1994).Photochemical protection research methods for plant protection professional use [M].Beijing:China Agriculture Press:39-45.(in Chinese)
[15] 王璐瑶,仇智灵,姚海峰,等.甘薯茎腐病菌室内药剂毒力筛选试验[J].浙江农业科学,2018,59(2):300-304,321.WANG Lu-yao,QIU Zhi-ling,YAO Hai-feng,et al.(2018) Laboratory toxicity screening Test of Sweet Potato Stem Rot pathogen [J].Journal of Zhejiang Agricultural Sciences,59(2):300-304,321.(in Chinese)
[16] Baussanne,I.,Antoine,Bussière,Halder S.,et al.(2010).Synthesis and antimicrobial evaluation of amphiphilic neamine derivatives.Journal of Medicinal Chemistry,53(1):119-127.
[17] 郭近,范坤成,沈海峰,等.含噻唑锌和辛菌胺醋酸盐的组合物及其制剂和应用[P].2017.GUO Jin,FAN Kun-cheng,SHENG Hai-feng.(2017).Compositions containing zinc thiazole and octylamine acetate and their preparations and applications [P].(in Chinese)
[18] 汤小美,林天元,周珊珊,等.梨树根腐病病原菌的分离与鉴定及有效防控药剂筛选[J].南京农业大学学报,2017,(1):76-83.TANG Xiao-mei,LIN Tian-yuan,ZHOU Shan-shan,et al.(2017).Pathogen identification of root rot in pear plant andfungicide screening for its efficient control [J].Journal of Nanjing Agricultural University,(1):76-83.(in Chinese)
[19] 华乃震.农药微乳剂的研究和进展[J].现代农药,2004,3(5):19-23.HUA Nai-zhen.(2004).Research and development of pesticide microemulsion [J].Modern Agrochemicals,3(5):19-23.(in Chinese)
[20] Mingjing,Q.,Zhaojun,H.,Xinjun,X.,& Lina,Y..(2003).Triazophos resistance mechanisms in the rice stem borer (chilo suppressalis walker).Pesticide Biochemistry and Physiology,77(3):99-105.
[21] 范可地,麻理亚,胡益福.3%噻霉酮可湿性粉剂及其复配剂防治水稻细菌性条斑病药效试验[J].现代农业科技,2018,(14):120-122.FAN Ke-de,MA Li-ya,HU Yi-fu.(2018).Efficacy of 3% thiamethoxone wettable powder and its mixture in the control of bacterial stripe disease in rice [J].Modern Agricultural Technology,(14):120-122 .(in Chinese)
[22] 张惠淳,杨金琛,王立石,等.乙蒜素乳油防治黄瓜细菌性角斑病[J].吉林蔬菜,2007,(2):40-41.ZHANG Hui-chun,YANG Jin-chen,WANG Ii-shi.(2007).Ethyl allicin cream for the prevention and treatment of bacterial keratosis of cucumber [J].Jilin Vegetables,(2):40-41.(in Chinese)
[23] 曹涤环.广谱高效杀菌剂-乙蒜素[J].农村百事通,2014,(1):47-48.CAO Di-huan.(2014).Broad-spectrum highly effective fungicide - ethalin [J].Rural Bestong,(1):47-48.(in Chinese)
[24] 周艳丽.壬菌铜与乙蒜素增效复配制剂研究[D].杨凌:西北农林科技大学硕士论文,2014.ZHOU Yan-li.(2014).Study on synergistic preparation of copper-ethylallicin from nonbacterium [D].Master Dissertation.Northwest A&F University,Yangling.(in Chinese)
[25] Suresh,A.,Pallavi,P.,Srinivas,P.,Kumar,V.P.,Chandra,S.J.,& Reddy,S.R..(2010).Plant growth promoting activities of fluorescent pseudomonads associated with some crop plants.African Journal of Microbiology Research,4(14):1,491-1,494.
[26] 岳东霞,张要武,陈融,等.荧光假单胞菌工程菌株的构建及对黄瓜枯萎病的防治效果[J].华北农学报,2008,23(6):101-104.YUE Dong-xia,ZHANG Yao-wu,CHEN Rong,et al.(2008).Construction of Engineered Strains of Pseudomonas fluorescenceand Its Control Effects on Cucumber Fusarium Wilt Disease [J].North China agricultural journal,23(6):101-104.(in Chinese)
[27]董国菊.荧光假单胞菌Pseudomonas fluorescens P-72-10菌株对烟草黑胫病的生防机理研究[D].重庆:西南大学博士学位论文,2012.DONG Guo-ju.(2012).Biocontrol Mechanisms of Pseudomonas fluorescens strain P-72-10 on Black ShankDisease of Tobacco [D].Southwest university of China,Chongqing.(in Chinese)
[28] 黄成文,王希东,刘君,等.两种表达载体对西瓜食酸菌生物学特性的影响[J].新疆农业科学,2018,55(3):457-467.HUANG Cheng-wen,WANG Xi-dong,LIU Jun.(2018).Effects of Two types of Expression Vectors on Biological Characteristics of Acidovorax citrulli [J].Xinjiang Agricultural Sciences,55(3):457-467.(in Chinese)
[2] Zwet,T.V.D.,& Keil,H.L.(1979).Fire blight,a bacterial disease of Rosaceous plants..Fire blight :a bacterial disease of rosaceous plants.U.S.Dept.of Agriculture.
[3] Cabrefiga,J.,& Montesinos,E.(2005).Analysis of aggressiveness of erwinia amylovora using disease-dose and time relationships.Phytopathology,95(12):1,430.
[4]胡白石.梨火疫病菌的风险分析及检测技术研究[J].南京:南京农业大学博士论文,2000,HU Bai-shi.(2000).Study on risk analysis and detection technology of Erwinia amylovora [J].PhD Dissertation.Nanjing Agricultural University,Nanjing.(in Chinese)
[5]胡白石,许志刚,周国梁,等.梨火疫病的进境风险分析[J].植物保护学报,2001,(4):303-308.HUA Bai-shi,XU Zhi-gang,ZHOU Guo-liang,et al.(2001).Risk analysis of pear fire blight in China [J].Journal of Plant Protection,(4):303-308.(in Chinese)
[6] Michele,G.,Urs,K.,Matthias,M.,Monika,M.,& Isabel,H.G.(2015).Fire blight control:the struggle goes on.a comparison of different fire blight control methods in switzerland with respect to biosafety,efficacy and durability.International Journal of Environmental Research and Public Health,12(9):11,422-11,447.
[7] Mcghee,G.C.,Guasco,J.,Bellomo,L.M.,Blumer-Schuette,S.E.,Shane,W.W.,& Irish-Brown,A.,et al.(2011).Genetic analysis of streptomycin-resistant (sm-r) strains of erwinia amylovora suggests that dissemination of two genotypes is responsible for the current distribution of sm-r e.amylovora in michigan.Phytopathology,101(2):182.
[8] Tancos,K.A.,Cox,K.D.Effects of consecutive streptomycin and kasugamycin applications on epiphyticbacteria in the apple phyllosphere.[J].Plant disease.2017,101(1):158-164.
[9] Johnson,K.B.(1993).Effect of antagonistic bacteria on establishment of honey bee-dispersed erwinia amylovora in pear blossoms and on fire blight control.Phytopathology,83(7):995-1,002.
[10] Johnson,K.B.,Stockwell,V.O.,Burgett,D.M.,Sugar,D.,& Loper,J.E.(1993).Dispersal of erwinia amylovora and pseudomonas fluorescens by honey bees from hives to apple and pear blossoms.Phytopathology,83(5):478-484.
[11] Lindow,S.E.,Mcgourty,G.,& Elkins,R.(1996).Interactions of antibiotics with pseudomonas fluorescens strain a506 in the control of fire blight and frost injury to pear.Phytopathology,86(8):841-848.
[12] Lehman S M .Development of a bacteriophage-based biopesticide for fire blight [J].Brock University,2007.
[13] 王钊.安祖花细菌性枯萎病病原菌的分子检测与药剂室内筛选[D].北京:中国农业科学院硕士论文,2013.WANG Zhao .(2013).Detection of Xanthomonas axonopodis pv.dieffenbachiae in Authurium andreanum and Laboratory Screening of bactericides [D].Master Dissertation.Chinese Academy of Agricultural Sciences,Beijing.(in Chinese)
[14] 慕立义.植物化学保护研究方法[M].北京:中国农业出版社,1994:39-45.MU Li -yi.(1994).Photochemical protection research methods for plant protection professional use [M].Beijing:China Agriculture Press:39-45.(in Chinese)
[15] 王璐瑶,仇智灵,姚海峰,等.甘薯茎腐病菌室内药剂毒力筛选试验[J].浙江农业科学,2018,59(2):300-304,321.WANG Lu-yao,QIU Zhi-ling,YAO Hai-feng,et al.(2018) Laboratory toxicity screening Test of Sweet Potato Stem Rot pathogen [J].Journal of Zhejiang Agricultural Sciences,59(2):300-304,321.(in Chinese)
[16] Baussanne,I.,Antoine,Bussière,Halder S.,et al.(2010).Synthesis and antimicrobial evaluation of amphiphilic neamine derivatives.Journal of Medicinal Chemistry,53(1):119-127.
[17] 郭近,范坤成,沈海峰,等.含噻唑锌和辛菌胺醋酸盐的组合物及其制剂和应用[P].2017.GUO Jin,FAN Kun-cheng,SHENG Hai-feng.(2017).Compositions containing zinc thiazole and octylamine acetate and their preparations and applications [P].(in Chinese)
[18] 汤小美,林天元,周珊珊,等.梨树根腐病病原菌的分离与鉴定及有效防控药剂筛选[J].南京农业大学学报,2017,(1):76-83.TANG Xiao-mei,LIN Tian-yuan,ZHOU Shan-shan,et al.(2017).Pathogen identification of root rot in pear plant andfungicide screening for its efficient control [J].Journal of Nanjing Agricultural University,(1):76-83.(in Chinese)
[19] 华乃震.农药微乳剂的研究和进展[J].现代农药,2004,3(5):19-23.HUA Nai-zhen.(2004).Research and development of pesticide microemulsion [J].Modern Agrochemicals,3(5):19-23.(in Chinese)
[20] Mingjing,Q.,Zhaojun,H.,Xinjun,X.,& Lina,Y..(2003).Triazophos resistance mechanisms in the rice stem borer (chilo suppressalis walker).Pesticide Biochemistry and Physiology,77(3):99-105.
[21] 范可地,麻理亚,胡益福.3%噻霉酮可湿性粉剂及其复配剂防治水稻细菌性条斑病药效试验[J].现代农业科技,2018,(14):120-122.FAN Ke-de,MA Li-ya,HU Yi-fu.(2018).Efficacy of 3% thiamethoxone wettable powder and its mixture in the control of bacterial stripe disease in rice [J].Modern Agricultural Technology,(14):120-122 .(in Chinese)
[22] 张惠淳,杨金琛,王立石,等.乙蒜素乳油防治黄瓜细菌性角斑病[J].吉林蔬菜,2007,(2):40-41.ZHANG Hui-chun,YANG Jin-chen,WANG Ii-shi.(2007).Ethyl allicin cream for the prevention and treatment of bacterial keratosis of cucumber [J].Jilin Vegetables,(2):40-41.(in Chinese)
[23] 曹涤环.广谱高效杀菌剂-乙蒜素[J].农村百事通,2014,(1):47-48.CAO Di-huan.(2014).Broad-spectrum highly effective fungicide - ethalin [J].Rural Bestong,(1):47-48.(in Chinese)
[24] 周艳丽.壬菌铜与乙蒜素增效复配制剂研究[D].杨凌:西北农林科技大学硕士论文,2014.ZHOU Yan-li.(2014).Study on synergistic preparation of copper-ethylallicin from nonbacterium [D].Master Dissertation.Northwest A&F University,Yangling.(in Chinese)
[25] Suresh,A.,Pallavi,P.,Srinivas,P.,Kumar,V.P.,Chandra,S.J.,& Reddy,S.R..(2010).Plant growth promoting activities of fluorescent pseudomonads associated with some crop plants.African Journal of Microbiology Research,4(14):1,491-1,494.
[26] 岳东霞,张要武,陈融,等.荧光假单胞菌工程菌株的构建及对黄瓜枯萎病的防治效果[J].华北农学报,2008,23(6):101-104.YUE Dong-xia,ZHANG Yao-wu,CHEN Rong,et al.(2008).Construction of Engineered Strains of Pseudomonas fluorescenceand Its Control Effects on Cucumber Fusarium Wilt Disease [J].North China agricultural journal,23(6):101-104.(in Chinese)
[27]董国菊.荧光假单胞菌Pseudomonas fluorescens P-72-10菌株对烟草黑胫病的生防机理研究[D].重庆:西南大学博士学位论文,2012.DONG Guo-ju.(2012).Biocontrol Mechanisms of Pseudomonas fluorescens strain P-72-10 on Black ShankDisease of Tobacco [D].Southwest university of China,Chongqing.(in Chinese)
[28] 黄成文,王希东,刘君,等.两种表达载体对西瓜食酸菌生物学特性的影响[J].新疆农业科学,2018,55(3):457-467.HUANG Cheng-wen,WANG Xi-dong,LIU Jun.(2018).Effects of Two types of Expression Vectors on Biological Characteristics of Acidovorax citrulli [J].Xinjiang Agricultural Sciences,55(3):457-467.(in Chinese)