43.5%吡虫·氟虫腈悬浮种衣剂的高效液相色谱分析
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摘要
建立一种高效液相色谱法,对43.5%吡虫·氟虫腈悬浮种衣剂中有效成分进行分离和定量分析。方法使用HYPERSIL BDS-C18色谱柱和紫外可变波长检测器,以甲醇+水为流动相,在268nm波长下采用外标法定量。氟虫腈的标准偏差为0.045 3,变异系数为0.32%,回收率为98.3%~100.3%;吡虫啉的标准偏差为0.107 9,变异系数为0.37%,回收率为99.6%~100.2%。
A HPLC method for the determination of active ingredients in imidacloprid + fipronil 43.5% FS was established. The simulataneous determination of fipronil and imidacloprid in the samples was conducted with HYPERSIL BDS-C18 column and UV-absorbance detector, while the mobile phase was a mixture of methanol and water, and the determine wavelength was 268 nm. The results showed that the standard deviation of fipronil was 0.045 3, the coefficient of variation was 0.32%, and the recoveries were 98.3%-100.3%. The standard deviation of imidacloprid was 0.107 9, the coefficient of variation was 0.37%, and the recoveries were 99.6%-100.2%.
A HPLC method for the determination of active ingredients in imidacloprid + fipronil 43.5% FS was established. The simulataneous determination of fipronil and imidacloprid in the samples was conducted with HYPERSIL BDS-C18 column and UV-absorbance detector, while the mobile phase was a mixture of methanol and water, and the determine wavelength was 268 nm. The results showed that the standard deviation of fipronil was 0.045 3, the coefficient of variation was 0.32%, and the recoveries were 98.3%-100.3%. The standard deviation of imidacloprid was 0.107 9, the coefficient of variation was 0.37%, and the recoveries were 99.6%-100.2%.
引文
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[4]王丹丹,丁明祥.吡虫啉母药的高效液相色谱分析[J].农药,2010,49(8):576-577.
[5]许行义,潘荷芳,刘劲松,等.气相色谱法测定水中吡虫啉和氟虫腈[J].环境监测管理与技术,2011,23(4):43-45.
[2]王玉玲,史常春,李自华,等.氟虫腈高效液相色谱分析方法优化[J].农药,2014,53(8):577-578.
[3]刁传芸,孙立荣,常有宏,等.梨中吡虫啉的高效液相色谱分析[J].江苏农业学报,2007,23(6):638-641.
[4]王丹丹,丁明祥.吡虫啉母药的高效液相色谱分析[J].农药,2010,49(8):576-577.
[5]许行义,潘荷芳,刘劲松,等.气相色谱法测定水中吡虫啉和氟虫腈[J].环境监测管理与技术,2011,23(4):43-45.