菊酯农药微生物降解的异构体选择性特征
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摘要
从农药厂排放污泥中筛选分离得到1株可以以甲氰菊酯为唯一碳源生长的细菌菌株W-10,经鉴定为粪产碱杆菌。考察其对甲氰菊酯(FP)、氯菊酯(PM)和高效氟氯氰菊酯(β-CF)的降解特征,降解半衰期分别为5.06、3.40、4.03 d,重点研究菌株W-10对不同异构体和对映体的降解差异。结果表明,3种菊酯虽然结构相近,但氯菊酯和高效氟氯氰菊酯的顺反异构体呈现不同的降解选择性,菌株W-10分别优先降解氯菊酯的顺式体和高效氟氯氰菊酯的反式体;此外,菌株W-10对对映体的降解选择性则主要体现在高效氟氯氰菊酯反式异构体所包含的一对对映体上。
引文
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[3]Chen S H,Hu M Y,Liu J J,et al.Biodegradation of beta-cypermethrin and 3-phenoxybenzoic acid by a novel Ochrobactrum lupini DG-S-01[J].Journal of Hazardous Materials,2011,187(1/2/3):433-440.
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[5]Liu W P,Gan J Y,Schlenk D,et al.Enantioselectivity in environmental safety of current chiral insecticides[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(3):701-706.
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[7]Sakata S,Mikami N,Yamada H.Degradation of pyrethroid optical isomers in soils[J].Journal of Pesticide Science,1992,17(3):169-180.
[8]Liu W P,Gan J J,Lee S,et al.Isomer selectivity in aquatic toxicity and biodegradation of cypermethrin[J].Journal of Agricultural and Food Chemistry,2004,52(20):6233-6238.
[9]Liu W P,Gan J Y,Lee S,et al.Isomer selectivity in aquatic toxicity and biodegradation of bifenthrin and permethrin[J].Environmental Toxicology and Chemistry,2005,24(8):1861-1866.
[10]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:370-410.
[11]奥斯伯F M,布伦特R,金斯顿R E,等.精编分子生物学实验指南[M].5版.金由辛,包慧中,赵丽云,等译.北京:科学出版社,2001.
[12]李森,田晶,李朝阳,等.氯菊酯微生物手性降解的研究[J].江苏农业科学,2017,45(20):282-284.
[13]Qin S J,Gan J.Enantiomeric differences in permethrin degradation pathways in soil and sediment[J].Journal of Agricultural and Food Chemistry,2006,54(24):9145-9151.
[14]Li Z Y,Luo X N,Li Q L,et al.Stereo and enantioselective separation and identification of synthetic pyrethroids,and photolytical isomerization analysis[J].Bulletin of Environmental Contamination and Toxicology,2015,94(2):254-259.