Desulfovibrio desulfuricans G20生理特性及处理含铬(Ⅵ)硫酸盐废水的研究
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摘要
选取一株硫酸盐还原菌Desulfovibrio desulfuricans G20,探讨其生理特性及其对含重金属硫酸盐废水的处理效果。结果显示,D.desulfuricans G20在2~18 h进入对数生长期,18~26.5 h进入稳定期。该菌株最适宜温度为37℃,最佳初始p H为8.2;最佳生长碳源为乳酸钠和蔗糖;最适硫源为亚硫酸钠。D.desulfuricans G20对Cr~(6+)最大耐受度为150 mg/L。随着Cr~(6+)初始浓度的减少,SO2-4去除率逐渐增加,最高达75.67%。Cr~(6+)质量浓度低于120 mg/L的去除率接近100%。可见,D.desulfuricans G20有潜力应用于处理含重金属Cr~(6+)的硫酸盐废水。
We developed a microbial process to remediate heavy metals from waste water. Asulfatereducing bacterium Desulfovibrio desulfuricans G20 was selected to study its growth and removal of heavy metal from wastewater.We studied the growth conditions of the bacterium and its removal ability of SO_4~(2-) and Cr~(6+). D. desulfuricans G20 entered the logarithmic growth phase between 2 and 18 h,stationaryphase between 18 and 26. 5 h. The optimal temperature was 37 ℃. The optimum initial pH was 8. 2. The best carbon sourceswere sodium lactate and sucrose. The optimum sulfur source was sodium sulfite. The maximum tolerance of D. desulfuricans G20 to Cr~(6+)was 150 mg/L. With the increase of the concentration of Cr~(6+),the removal of SO_4~(2-) decreased gradually. The maximum removal of SO_4~(2-) was 75. 67%. When the initial concentration of Cr~(6+)was less than 30 mg/L,removal of Cr~(6+)was close to 100%. D. desulfuricans G20 has potential to treat wastewater containing heavy metal sulfate.
We developed a microbial process to remediate heavy metals from waste water. Asulfatereducing bacterium Desulfovibrio desulfuricans G20 was selected to study its growth and removal of heavy metal from wastewater.We studied the growth conditions of the bacterium and its removal ability of SO_4~(2-) and Cr~(6+). D. desulfuricans G20 entered the logarithmic growth phase between 2 and 18 h,stationaryphase between 18 and 26. 5 h. The optimal temperature was 37 ℃. The optimum initial pH was 8. 2. The best carbon sourceswere sodium lactate and sucrose. The optimum sulfur source was sodium sulfite. The maximum tolerance of D. desulfuricans G20 to Cr~(6+)was 150 mg/L. With the increase of the concentration of Cr~(6+),the removal of SO_4~(2-) decreased gradually. The maximum removal of SO_4~(2-) was 75. 67%. When the initial concentration of Cr~(6+)was less than 30 mg/L,removal of Cr~(6+)was close to 100%. D. desulfuricans G20 has potential to treat wastewater containing heavy metal sulfate.
引文
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[24]王谦,李延,孙平,等.含铬废水处理技术及研究进展[J].环境科学与技术,2013,36(12):150-156.
[25]张莹,章莹颖,唐杰,等.微小杆菌Exiguobacterium sp.MH3对六价铬的还原特性[J].应用与环境生物学报,2014,20(5):791-797.
[26]KANMANI P,ARAVIND J,PRESTON D.Remediation of chromium contaminants using bacteria[J].Int J Environ Sci Technol,2011,9(1):183-193.
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[28]FERRO OROZCO A M,CONTRERAS E M,ZARITZKY N E.Cr(VI)reduction capacity of activated sludge as affected by nitrogen and carbon sources,microbial acclimation and cell multiplication[J].J Hazard Mat,2010,176(1/2/3):657-665.
[29]ALAM M Z,MALIK A.Chromate resistance,transport and bioreduction by Exiguobacterium sp.ZM-2 isolated from agricultural soil irrigated with tannery effluent[J].J Basic Microbiol,2008,48(5):416-420.
[30]ALAM M Z,AHMAD S.Chromium removal through biosorption and bioaccumulation by bacteria from tannery effluents contaminated soil[J].CLEAN-Soil,Air,Water,2011,39(3):226-237.
[31]李福德,李昕,谢翼飞,等.微生物去除重金属和砷:复合硫酸盐还原菌法的机理与技术[M].北京:化学工业出版社,2011.
[32]LAXMAN R S,MORE S.Reduction of hexavalent chromium by Streptomyces griseus[J].Minerals Eng,2002,15(11):831-837.
[33]THACKER U,PARIKH R,SHOUCHE Y,et al.Reduction of chromate by cell-free extract of Brucella sp.isolated from Cr(VI)contaminated sites[J].Bioresour Technol,2007,98(8):1541-1547.
[2]MUYZER G,STAMS AJ M.The ecology and biotechnology of sulphate-reducing bacteria[J].Nat Rev Microb,2008,6(6):441-454.
[3]ANANDKUMAR B,GEORGE R P,MARUTHAMUTHUS,et al.Corrosion behavior of SRB Desulfobulbus propionicus isolated from an Indian petroleum refinery on mild steel[J].Mater Corros,2012,63(4):355-362.
[4]张子栋.六价铬毒性作用及其影响因素[J].生物技术世界,2013(8):71.
[5]闫旭,李亚峰.含铬废水的处理方法[J].辽宁化工,2010,39(2):143-146.
[6]任军俊,肖利萍.硫酸盐还原菌处理废水的研究进展与展望[J].水资源与水工程学报,2009,20(2):52-56.
[7]安文辉,马斯然,王磊峰.硫酸盐还原菌处理含铬废水的研究现状[J].广东化工,2012,17(39):97-98.
[8]贺气志,陈辉,王丹,等.混合硫酸盐还原菌与Cu/Fe颗粒协同处理含铬废水的研究[J].环境科学,2011,32(7):2000-2005.
[9]陈炜婷,张鸿郭,罗定贵,等.硫酸盐还原菌处理含重金属酸性废水的研究[J].安徽农业科学,2013,42(31):12422-12424.
[10]李猛,张鸿郭,周子倩,等.固定化SRB处理低浓度含铬废水[J].环境工程,2016(4):20-24.
[11]王婷,周俊,徐为中,等.生长因子对SRB处理硫酸盐废水的影响研究[J].工业水处理,2016,36(6):38-42.
[12]任南琪,王爱杰,赵阳国.废水厌氧处理硫酸盐还原菌生态学[M].北京:科学出版社,2009.
[13]胡永娟,李学梅,王海磊,等.抗铬菌株的筛选鉴定及其生物学特性和吸附特性[J].环境工程学报,2014,8(6):2585-2591.
[14]梁宇.硫酸盐还原菌的生长因子的探讨[J].山西建筑,2010(30):199-200.
[15]王辉,戴友芝,刘川,等.混合硫酸盐还原菌代谢过程的影响因素[J].环境工程学报,2012,6(6):1795-800.
[16]陈炜婷,张鸿郭,陈永亨,等.p H、温度及初始铊浓度对硫酸盐还原菌脱铊的影响[J].环境工程学报,2014,10(8):4105-4109.
[17]许雅玲,伍健东.p H值对硫酸盐还原菌颗粒污泥性能的影响[J].工业用水与废水,2010,4(1):32-35.
[18]韩煦,周天旭,刘勇.不同碳源对ZVI-SRB-SO2-4体系中生物活性的影响[J].天津工业大学学报,2015,34(2):58-63.
[19]马忠友,邓盾,汪建飞,等.一组混合菌群还原硫酸盐的特性[J].中国农学通报,2013,29(8):184-188.
[20]肖利萍,张镭,李月.硫酸盐还原菌及其在废水厌氧治理中的应用[J].水资源与水工程学报,2011,22(1):45-48.
[21]郑家传,张建荣,刘希雯,等.污染场地六价铬的还原和微生物稳定化研究[J].环境科学,2014,35(10):3882-3887.
[22]徐莲,孙纪全,吴晓磊,等.菌株Rhodococcus sp.Chr-9和Exiguobacterium sp.Chr-43的除铬(VI)特性[J].应用与环境生物学报,2012,18(6):971-977.
[23]秦利玲,王天贵.用细菌解毒水溶液中六价铬的实验研究[J].化学工程师,2010(10):37-41.
[24]王谦,李延,孙平,等.含铬废水处理技术及研究进展[J].环境科学与技术,2013,36(12):150-156.
[25]张莹,章莹颖,唐杰,等.微小杆菌Exiguobacterium sp.MH3对六价铬的还原特性[J].应用与环境生物学报,2014,20(5):791-797.
[26]KANMANI P,ARAVIND J,PRESTON D.Remediation of chromium contaminants using bacteria[J].Int J Environ Sci Technol,2011,9(1):183-193.
[27]SARANGI A,KRISHNAN C.Comparison of in vitro Cr(VI)reduction by CFEs of chromate resistant bacteria isolated from chromate contaminated soil[J].Bioresour Technol,2008,99(10):4130-4137.
[28]FERRO OROZCO A M,CONTRERAS E M,ZARITZKY N E.Cr(VI)reduction capacity of activated sludge as affected by nitrogen and carbon sources,microbial acclimation and cell multiplication[J].J Hazard Mat,2010,176(1/2/3):657-665.
[29]ALAM M Z,MALIK A.Chromate resistance,transport and bioreduction by Exiguobacterium sp.ZM-2 isolated from agricultural soil irrigated with tannery effluent[J].J Basic Microbiol,2008,48(5):416-420.
[30]ALAM M Z,AHMAD S.Chromium removal through biosorption and bioaccumulation by bacteria from tannery effluents contaminated soil[J].CLEAN-Soil,Air,Water,2011,39(3):226-237.
[31]李福德,李昕,谢翼飞,等.微生物去除重金属和砷:复合硫酸盐还原菌法的机理与技术[M].北京:化学工业出版社,2011.
[32]LAXMAN R S,MORE S.Reduction of hexavalent chromium by Streptomyces griseus[J].Minerals Eng,2002,15(11):831-837.
[33]THACKER U,PARIKH R,SHOUCHE Y,et al.Reduction of chromate by cell-free extract of Brucella sp.isolated from Cr(VI)contaminated sites[J].Bioresour Technol,2007,98(8):1541-1547.