穴位贴敷诱发的急、慢性接触性皮炎皮损特点及胸腺基质淋巴细胞生成素的表达差异性
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摘要
目的观察穴位贴敷诱发的急性接触性皮炎与慢性接触性皮炎皮损特点以及皮损内胸腺基质淋巴细胞生成素(thymal stromal,tymphopoietin,TSLP)表达的差异性。方法选取三伏天接受穴位贴敷治疗哮喘且诱发急性或慢性接触性皮炎的患者20例,取皮分为急性接触性皮炎组(简称急性组)和慢性接触性皮炎组(简称慢性组),观察两组患者皮损特点及皮损组织中TSLP表达的差异性。结果急性组有大量的TSLP分布,弥漫性分布在表皮层(角质层、颗粒层、棘层和基底层)以及真皮层血管周围;慢性组TSLP数量较急性组少,集中分布在表皮层的角质层、棘层以及真皮层血管周围。结论 TSLP在穴位贴敷诱发的急性接触性皮炎与慢性接触性皮炎中的分布具有明显的数量差异性,而分布部位差异性不明显。推测皮炎性质(急性或慢性)会影响穴位贴敷疗效,而TSLP可以作为一个监测指标。
Objective To observe the characteristics of acute contact dermatitis and chronic contact dermatitis induced by acupoint application and the differential expression of thymic stromal lymphopoietin(TSLP) in the lesions. Methods A total of 20 asthma patients with acute or chronic contact dermatitis who received acupoint application in our department were selected. The patients was divided into acute contact dermatitis group(abbreviated as acute group) and chronic contact dermatitis group(referred to as chronic group). Observed the characteristics of acute contact dermatitis and chronic contact dermatitis induced by acupoint application and the differential expression of thymic stromal lymphopoietin(TSLP) in the lesions. Results 1.There was a great distribution of TSLP in the acute group, and the diffuse distribution was distributed around the epidermis( stratum corneum, granular layer, ratchet layer and basal layer) and perivascular vessels; 2.The number of TSLP in the chronic group was less than that in the acute group, and concentrated in the stratum corneum, the ratchet layer and the dermis of the epidermis. Conclusion The distribution of TSLP in acute contact dermatitis and chronic contact dermatitis induced by acupoint application has a significant quantitative difference, but the distribution site is not significantly different. It is speculated that the nature of dermatitis(acute or chronic) affects the therapeutic effect of acupoint application, and TSLP can be used as a monitoring index.
Objective To observe the characteristics of acute contact dermatitis and chronic contact dermatitis induced by acupoint application and the differential expression of thymic stromal lymphopoietin(TSLP) in the lesions. Methods A total of 20 asthma patients with acute or chronic contact dermatitis who received acupoint application in our department were selected. The patients was divided into acute contact dermatitis group(abbreviated as acute group) and chronic contact dermatitis group(referred to as chronic group). Observed the characteristics of acute contact dermatitis and chronic contact dermatitis induced by acupoint application and the differential expression of thymic stromal lymphopoietin(TSLP) in the lesions. Results 1.There was a great distribution of TSLP in the acute group, and the diffuse distribution was distributed around the epidermis( stratum corneum, granular layer, ratchet layer and basal layer) and perivascular vessels; 2.The number of TSLP in the chronic group was less than that in the acute group, and concentrated in the stratum corneum, the ratchet layer and the dermis of the epidermis. Conclusion The distribution of TSLP in acute contact dermatitis and chronic contact dermatitis induced by acupoint application has a significant quantitative difference, but the distribution site is not significantly different. It is speculated that the nature of dermatitis(acute or chronic) affects the therapeutic effect of acupoint application, and TSLP can be used as a monitoring index.
引文
[1] 陈卫华,辛坤,蔡臣安,等.中药穴位贴敷治疗支气管哮喘疗效观察[J].中国针灸,2009,29(4):272.
[2] Lu LS,Wang MM,Chen HL.Point Application of Medicated Cakes for Treatment of Asthma in Children-A Report of 60 Cases[J].J Tradit Chin Med.2006,26(3):165.
[3] 刘兰英,胡骏,王和生,等.穴位贴敷致急性接触性皮炎1例[J].中国皮肤性病学杂志,2017,31(7):813.
[4] 王晓萌,张理涛.中药三伏贴所致痒疹样接触性皮炎1例[J].中国中西医结合皮肤性病学杂志,2014,11(1):17.
[5] 刘小晓,江夏,彭科,等.三伏贴所致复发性接触性皮炎1例[J].中国中西医结合皮肤性病学杂志,2017,16(6):538.
[6] 赵辨.中国临床皮肤病学[M].南京:江苏科学技术出版社,2010:717.
[7] Eric B.Brandt and Umasundari Sivaprasad.Th2 Cytokines and Atopic Dermatitis [J].J Clin Cell Immunol,2011,2(3):1.
[8] Liu YJ.Thymic stromal lymphopoietin:master switch for allergic infl ammation [J].JEM,2006,203(2):269.
[9] Herbertstrobl.Inflammatory Langerhans cell differentiation[J].Blood,2014,124(15):2319.
[10] Wang YH,Liu YJ.Thymic stromal lymphopoietin,OX40-ligand,and interleukin-25 in allergic responses[J].Clin Exp Allergy,2009,39(6):798.
[11] Seshasayee D,Lee WP,Zhou MJ,et al.In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation[J].Clin Invest,2007,117(12):3868.
[12] Marshall NB,Lukomska E,Long CM,et al.Triclosan Induces Thymic Stromal Lymphopoietin in Skin Promoting Th2 Allergic Responses [J].Toxicol Sci,2015,147(1):127.
[13] 中华医学会呼吸病学分会哮喘学组.支气管哮喘防治指南( 支气管哮喘的定义、诊断、治疗和管理方案)[J].中华结核和呼吸杂志,2008,31(3):177.
[14] 清·张璐.张氏医通[M].北京:中国中医药出版社,1995:85.
[15] Segawa R,Hirasawa N.Exacerbation of Allergic Diseases by Chemicals:Role of TSLP [J].J Pharmacol Sci,2014,124(3):301.
[16] Werfel T,Biedermann T.Current novel approaches in systemic therapy of atopic dermatitis:specific inhibition of cutaneous Th2 polarized inflammation and itch[J].Curr Opin Allergy Clin Immunol,2015,15(5):446.
[17] Satou N,Ishihara K,Hiratsuka M,et al.Induction of Thymic Stromal Lymphopoietin Production by Xylene and Exacerbation of Picryl Chloride-Induced Allergic Inflammation in Mice[J].Int Arch Allergy Immunol 2012,157:194.
[18] 袁保丰,秦黎虹,张建华.穴位贴敷次数对缓解期哮喘患者肺功能的影响[J].云南中医中药杂志,2007(9):9.
[19] 董征.消喘膏223例哮喘患10年疗效观察[J].中西医结合杂志,1998,18(6):336.
[20] 黄冬娥,郭健红,林莺.穴位贴敷治疗支气管哮喘时间因素的研究概况[J].中国针灸,2010,30(2):173.
[21] Demehri S,Liu Z,Lee J,et al.Notch-deficient skin induces a lethal systemic B-lymphoproliferative disorder by secreting TSLP,a sentinel for epidermal integrity[J].PLoS Biol,2008,6(5):e123.
[22] 陈卫华,辛坤,蔡臣安,等.中药穴位贴敷治疗支气管哮喘疗效观察[J].中国针灸,2009,29(4):272.
[2] Lu LS,Wang MM,Chen HL.Point Application of Medicated Cakes for Treatment of Asthma in Children-A Report of 60 Cases[J].J Tradit Chin Med.2006,26(3):165.
[3] 刘兰英,胡骏,王和生,等.穴位贴敷致急性接触性皮炎1例[J].中国皮肤性病学杂志,2017,31(7):813.
[4] 王晓萌,张理涛.中药三伏贴所致痒疹样接触性皮炎1例[J].中国中西医结合皮肤性病学杂志,2014,11(1):17.
[5] 刘小晓,江夏,彭科,等.三伏贴所致复发性接触性皮炎1例[J].中国中西医结合皮肤性病学杂志,2017,16(6):538.
[6] 赵辨.中国临床皮肤病学[M].南京:江苏科学技术出版社,2010:717.
[7] Eric B.Brandt and Umasundari Sivaprasad.Th2 Cytokines and Atopic Dermatitis [J].J Clin Cell Immunol,2011,2(3):1.
[8] Liu YJ.Thymic stromal lymphopoietin:master switch for allergic infl ammation [J].JEM,2006,203(2):269.
[9] Herbertstrobl.Inflammatory Langerhans cell differentiation[J].Blood,2014,124(15):2319.
[10] Wang YH,Liu YJ.Thymic stromal lymphopoietin,OX40-ligand,and interleukin-25 in allergic responses[J].Clin Exp Allergy,2009,39(6):798.
[11] Seshasayee D,Lee WP,Zhou MJ,et al.In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation[J].Clin Invest,2007,117(12):3868.
[12] Marshall NB,Lukomska E,Long CM,et al.Triclosan Induces Thymic Stromal Lymphopoietin in Skin Promoting Th2 Allergic Responses [J].Toxicol Sci,2015,147(1):127.
[13] 中华医学会呼吸病学分会哮喘学组.支气管哮喘防治指南( 支气管哮喘的定义、诊断、治疗和管理方案)[J].中华结核和呼吸杂志,2008,31(3):177.
[14] 清·张璐.张氏医通[M].北京:中国中医药出版社,1995:85.
[15] Segawa R,Hirasawa N.Exacerbation of Allergic Diseases by Chemicals:Role of TSLP [J].J Pharmacol Sci,2014,124(3):301.
[16] Werfel T,Biedermann T.Current novel approaches in systemic therapy of atopic dermatitis:specific inhibition of cutaneous Th2 polarized inflammation and itch[J].Curr Opin Allergy Clin Immunol,2015,15(5):446.
[17] Satou N,Ishihara K,Hiratsuka M,et al.Induction of Thymic Stromal Lymphopoietin Production by Xylene and Exacerbation of Picryl Chloride-Induced Allergic Inflammation in Mice[J].Int Arch Allergy Immunol 2012,157:194.
[18] 袁保丰,秦黎虹,张建华.穴位贴敷次数对缓解期哮喘患者肺功能的影响[J].云南中医中药杂志,2007(9):9.
[19] 董征.消喘膏223例哮喘患10年疗效观察[J].中西医结合杂志,1998,18(6):336.
[20] 黄冬娥,郭健红,林莺.穴位贴敷治疗支气管哮喘时间因素的研究概况[J].中国针灸,2010,30(2):173.
[21] Demehri S,Liu Z,Lee J,et al.Notch-deficient skin induces a lethal systemic B-lymphoproliferative disorder by secreting TSLP,a sentinel for epidermal integrity[J].PLoS Biol,2008,6(5):e123.
[22] 陈卫华,辛坤,蔡臣安,等.中药穴位贴敷治疗支气管哮喘疗效观察[J].中国针灸,2009,29(4):272.