PTPN11基因多态性及与细毛羊重要经济性状的关联分析
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摘要
为分析中国美利奴羊(新疆型)PTPN11基因的多态性及与细毛羊重要经济性状的相关性,寻找可用于中国美利奴羊(新疆型)选育的分子遗传标记。本研究运用PCR-SSCP技术检测PTPN11基因的多态性,用SAS 8.1软件中的最小二乘方法分析PTPN11基因与羊毛性状的关联性。结果表明,PTPN11基因共检测到了AA、AB和BB 3种基因型,其基因型频率分别为0.28、0.52和0.20;A、B等位基因频率分别为0.54和0.46,A等位基因占优势。χ~2检验显示,PTPN11基因处于哈代-温伯格平衡状态(P>0.05),PTPN11基因位点的多态信息含量为0.37,PTPN11基因在该群体中处于中等程度的遗传变异。不同基因型的个体对弯曲评分有显著影响(P<0.05),对其他性状均无显著影响(P>0.05)。
The aim of this study is to analyze the polymorphism of the Chinese Merino sheep(Xinjiang type) PTPN11 gene and its correlation with the important economic traits of the fine wool sheep,and to find the molecular genetic markers that can be used for the breeding of Chinese Merino sheep(Xinjiang type).In this study, PCR-SSCP was used to detect the polymorphism of PTPN11 gene,and the least squares method in SAS8.1 software was used to analyze the correlation between PTPN11 gene and wool traits. The results showed that the three genotypes of PTPN11 gene were detected as AA,AB and BB,and their genotype frequencies were 0.28,0.52 and 0.20,respectively. The allele frequencies of A and B were 0.54 and 0.46,respectively,A allele was the dominant allele.χ~2 test showed that PTPN11 gene loci was in Hardy Weinberg equilibrium state(P>0.05). The polymorphism information content of the PTPN11 gene was 0.37,therefore, the genetic variation of the Chinese Merino(Xinjiang type) sheep population was in the medium level(P <0.01).Individuals with different genotypes had significant effects on bending score(P <0.05),but had no significant effect on other traits(P >0.05).
The aim of this study is to analyze the polymorphism of the Chinese Merino sheep(Xinjiang type) PTPN11 gene and its correlation with the important economic traits of the fine wool sheep,and to find the molecular genetic markers that can be used for the breeding of Chinese Merino sheep(Xinjiang type).In this study, PCR-SSCP was used to detect the polymorphism of PTPN11 gene,and the least squares method in SAS8.1 software was used to analyze the correlation between PTPN11 gene and wool traits. The results showed that the three genotypes of PTPN11 gene were detected as AA,AB and BB,and their genotype frequencies were 0.28,0.52 and 0.20,respectively. The allele frequencies of A and B were 0.54 and 0.46,respectively,A allele was the dominant allele.χ~2 test showed that PTPN11 gene loci was in Hardy Weinberg equilibrium state(P>0.05). The polymorphism information content of the PTPN11 gene was 0.37,therefore, the genetic variation of the Chinese Merino(Xinjiang type) sheep population was in the medium level(P <0.01).Individuals with different genotypes had significant effects on bending score(P <0.05),but had no significant effect on other traits(P >0.05).
引文
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[3] Miyamoto D,Miyamoto M,Takahashi A,et al.Isolation of a distinct class of gain-of-function SHP-2 mutants with oncogenic RAS-like transforming activity from solid tumors[J].Oncogene,2008,27(25):3508-3515.
[4] 贺慧颖,郑杰,李燕,等.SHP2和MKP5在P2Y嘌呤受体介导的人前列腺癌细胞侵袭中的调控机制研究[J].中华病理学杂志,2005,34(5): 288-292.
[5] Liu X,Qu C K.Protein Tyrosine Phosphatase SHP-2 (PTPN11) in Hematopoiesis and Leukemogenesis[J].Journal of Signal Transduction,2011,2011:1-8.
[6] 李铁求.前列腺癌转移相关基因的生物信息学分析及功能预测[D].广州:南方医科大学,2014.
[7] Gordon C,Demetrios K,B G Neel.The tyrosine phosphatase SHP-2(PTPN11) in cancer[J].Cancer Metastasis Rev,2008,27:179-192.
[8] 虞瑛姿.SHP-2的表达与人白血病细胞恶性增殖的相关性研究[D].杭州:浙江大学,2005.
[9] Zhou X,Agazie Y M.Molecular Mechanism for SHP-2 in Promoting HER2-induced Signaling and Transformation[J].Journal of Biological Chemistry,2009,284(18):12226-12234.
[10] 赵宪坤.PTPN11基因3’UTR短串联重复序列与肝细胞肝癌易感性关联研究及初步功能分析[D].苏州:苏州大学,2015.
[11] 柏妍.细毛羊毛性状候选基因遗传效应的研究[D].乌鲁木齐:新疆农业大学,2015.
[12] 田月珍.中国美利奴羊(新疆型)母羊羊毛细度相关候选基因的筛选及其关联性分析[D].乌鲁木齐:新疆农业大学,2012.
[13] 艾买提.5个KAP基因在中国美利奴羊(新疆型)的多态性及其与毛性的关联性分析[D].乌鲁木齐:新疆农业大学,2013.
[14] 何军敏,黄锡霞,田可川,等.KAP16基因对细毛羊重要经济性状的遗传效应分析[J].西南农业学报,2017,30(2):458-465.
[15] 赵冰茹,黄锡霞,田可川,等.细毛羊LAMB1基因外显子多态性及其与羊毛纤维直径的关联性分析[J].中国畜牧兽医,2016,43(9):2401-2411.
[16] 梁秀兰,胡宏波,贾安平,等.胃癌与PTPN11基因多态性及幽门螺杆菌感染的相关性研究[J].右江民族医学院学报,2011,33(3):261-263.
[17] 张丽,王芃,梁浩,等.PTPN11第3内含子基因多态性与胃癌的相关性研究[J].解放军医学杂志,2011,36(5):474-477.