碳离子束辐照水稻诱变效应及突变体的筛选
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摘要
【目的】丰富水稻Oryza sativa L.突变体库,快速创造稳定遗传的新种质,为重离子辐照诱变机理研究和水稻遗传育种提供丰富的基础材料。【方法】利用碳离子束辐照水稻品种‘IR-55’、‘IR-60’、‘三粒寸’和‘云南陆稻’原种进行诱变,并对诱变后代的多个农艺性状进行多代跟踪调查。【结果】4个水稻品种突变株系株高、剑叶长、穗长和粒长宽比等性状呈现出较大变异幅度,且具有明显的正向或负向性,结实率表现出明显的下降趋势,最低为2%。利用随机选取的SSR分子标记引物对突变体进行遗传多样性检测发现,突变株系与原种的遗传多样性表现出明显的差异,表明重离子辐照诱变引起DNA序列较大片段的改变,而不是微小的点突变。【结论】碳离子束辐照对水稻株高、剑叶长和粒型等多个农艺性状产生正向或负向的诱变效应,在诱变后代中可以筛选出一批农艺性状变异的水稻新种质。
【Objective】To enrich rice(Oryza sativa L.) mutant library, rapidly create genetically stable new germplasm,and provide valuable materials for following study of mutation mechanisms and rice genetic breeding.【Method】The breeder seeds of ‘IR55', ‘IR60', ‘Sanlicun' and ‘Yunnan Ludao' were irradiated by carbon ion beam, and the agronomic traits of induced generations were consecutively investigated.【Result】The mutants of four cultivars varied over a large range of variation in plant height, flag leaf length, spikelet length,length-width ratio of grain and other traits, and displayed obvious positive or negative mutation. Seed setting rate decreased respectively with the lowest value of 2%. Large genetic diversity variations were detected between the mutants and breeder seed populations of four cultivars using randomly selected SSR markers,indicating that heavy ion irradiation induced big fragment variations at DNA sequences, rather than subtle site mutation.【Conclusion】Carbon ion beam irradiation can cause positive or negative mutation of plant height, flag leaf length, grain shape and other agronomic traits of rice. New rice germplasms with variation in agronomic traitscan be screened out from mutation progenies.
【Objective】To enrich rice(Oryza sativa L.) mutant library, rapidly create genetically stable new germplasm,and provide valuable materials for following study of mutation mechanisms and rice genetic breeding.【Method】The breeder seeds of ‘IR55', ‘IR60', ‘Sanlicun' and ‘Yunnan Ludao' were irradiated by carbon ion beam, and the agronomic traits of induced generations were consecutively investigated.【Result】The mutants of four cultivars varied over a large range of variation in plant height, flag leaf length, spikelet length,length-width ratio of grain and other traits, and displayed obvious positive or negative mutation. Seed setting rate decreased respectively with the lowest value of 2%. Large genetic diversity variations were detected between the mutants and breeder seed populations of four cultivars using randomly selected SSR markers,indicating that heavy ion irradiation induced big fragment variations at DNA sequences, rather than subtle site mutation.【Conclusion】Carbon ion beam irradiation can cause positive or negative mutation of plant height, flag leaf length, grain shape and other agronomic traits of rice. New rice germplasms with variation in agronomic traitscan be screened out from mutation progenies.
引文
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[9]刘青芳,余丽霞,杜艳,等.12C+6离子束辐照对小麦种子萌发、幼苗生长及存活的影响[J].辐射研究与辐射工艺学报,2013,31(1):26-30.
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[14]余丽霞,李文建,杜艳,等.碳离子束辐照大豆当代诱变效应及褐皮突变体的初步研究[J].辐射研究与辐射工艺学报,2014,32(2):020402(1-7).
[15]张秋英,余丽霞,李彦生,等.重离子束辐照大豆籽粒当代效应的初步研究[J].大豆科学,2013,32(5):587-590.
[16]王悦.重离子束注入大豆种子的诱变效应研究[D].长沙:湖南农业大学,2008.
[17]耿金鹏,李多芳,曹天光,等.重离子辐射玉米自交系田间性状的统计分析[J].玉米科学,2013,21(2):66-70.
[18]刘志生,陈吉法,范广华,等.等离子束玉米自交系选育初探[J].激光生物学报,2002,11(2):142-144.
[19]罗红兵,赵葵,郭继宇,等.重离子辐照玉米种子M1代诱变效应研究[J].原子核物理评论,2004,21(3):238-242.
[20]钱平平,侯岁稳,吴大利,等.12C6+重离子辐照大葱的生物学效应[J].辐射研究与辐射工艺学报,2007,25(4):211-215.
[21]孙兰弟,张颖聪,吴大利,等.12C6+离子辐照对4种农作物种子出苗率及幼苗生长的研究[J].原子核物理评论,2008,25(2):182-186.
[22]周利斌,李文建,曲颖,等.离子束辐照结合组织培养在植物诱变中的研究进展[J].辐射研究与辐射工艺学报,2007,25(4):232-236.
[23]庄楚雄,胡维民,梅曼彤.高能重离子辐射诱导水稻半矮生突变株系的RFLP分析[J].核农学报,1997,11(2):74-78.
[24]潘映红.论植物表型组和植物表型组学的概念与范畴[J].作物学报,2015,41(2):175-186.
[2]张小冰,郝建平,裴雁曦,等.辐射诱变甜荞高产突变体过氧化物酶活性及其同工酶的研究[J].华北农学报,1998,13(1):71-73.
[3]王子宁,郭北海,张艳敏,等.小麦糯性突变体的筛选[J].华北农学报,2002,17(3):14-19.
[4]HASE Y,YOSHIHARA R,NOZAWA S,et al.Mutagenic effects of carbon ions near the range end in plants[J].Mutat Res,2012,731(1/2):41-47.
[5]曲颖,李文建,周利斌,等.重离子辐射植物的诱变效应研究及应用[J].原子核物理评论,2007,24(4):294-298.
[6]李姝汶,王晓军,段翼远,等.12C6+高能重离子辐照大葱损伤及其分子生物学效应[J].原子核物理评论,2010,27(3):328-334.
[7]梁前进,胡玉莲,张根发.拟南芥(Arabidopsis thaliana)氮、碳离子注入诱变效应分析[J].生物物理学报,2002,18(2):251-255.
[8]王曙阳,李景华,姜伯玲,等.12C6+离子辐照对苜蓿M1代低温胁迫存活率、过氧化氢酶及过氧化物酶活性的影响[J].原子核物理评论,2014,31(3):411-415.
[9]刘青芳,余丽霞,杜艳,等.12C+6离子束辐照对小麦种子萌发、幼苗生长及存活的影响[J].辐射研究与辐射工艺学报,2013,31(1):26-30.
[10]ARASE S,HASE Y,ABE J,et al.Optimization of ionbeam irradiation for mutagenesis in soybean:Effects on plant growth and production of visibly altered mutants[J].Plant Biotechnol,2011,28(3):323-329.
[11]MIRRA K,IKEDA M,MATSUBARA A,et al.Os SPL14 promotes panicle branching and higher grain productivity in rice[J].Nat Genet,2010,42(6):545-549.
[12]叶俊,吴建国,杜婧,等.水稻“9311”突变体筛选和突变体库构建[J].作物学报,2006,32(10):1525-1529.
[13]YAMAGUCHI H,HASE Y,TANAKA A,et al.Mutagenic effects of ion beam irradiation on rice[J].Breed Sci,2009,59(2):169-177.
[14]余丽霞,李文建,杜艳,等.碳离子束辐照大豆当代诱变效应及褐皮突变体的初步研究[J].辐射研究与辐射工艺学报,2014,32(2):020402(1-7).
[15]张秋英,余丽霞,李彦生,等.重离子束辐照大豆籽粒当代效应的初步研究[J].大豆科学,2013,32(5):587-590.
[16]王悦.重离子束注入大豆种子的诱变效应研究[D].长沙:湖南农业大学,2008.
[17]耿金鹏,李多芳,曹天光,等.重离子辐射玉米自交系田间性状的统计分析[J].玉米科学,2013,21(2):66-70.
[18]刘志生,陈吉法,范广华,等.等离子束玉米自交系选育初探[J].激光生物学报,2002,11(2):142-144.
[19]罗红兵,赵葵,郭继宇,等.重离子辐照玉米种子M1代诱变效应研究[J].原子核物理评论,2004,21(3):238-242.
[20]钱平平,侯岁稳,吴大利,等.12C6+重离子辐照大葱的生物学效应[J].辐射研究与辐射工艺学报,2007,25(4):211-215.
[21]孙兰弟,张颖聪,吴大利,等.12C6+离子辐照对4种农作物种子出苗率及幼苗生长的研究[J].原子核物理评论,2008,25(2):182-186.
[22]周利斌,李文建,曲颖,等.离子束辐照结合组织培养在植物诱变中的研究进展[J].辐射研究与辐射工艺学报,2007,25(4):232-236.
[23]庄楚雄,胡维民,梅曼彤.高能重离子辐射诱导水稻半矮生突变株系的RFLP分析[J].核农学报,1997,11(2):74-78.
[24]潘映红.论植物表型组和植物表型组学的概念与范畴[J].作物学报,2015,41(2):175-186.