不同产地枳壳药材中柠檬苦素、诺米林含量测定及聚类分析
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摘要
目的通过对不同产地的枳壳中活性成分柠檬苦素及诺米林进行含量比较,为不同产地枳壳药材品质评价提供依据。方法采用HPLC测定含量,色谱柱为C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水-四氢呋喃-冰醋酸(41∶59∶1∶0.2),流速1.0 m L·min-1,检测波长为225 nm。并以柠檬苦素、诺米林为指标进行聚类分析。结果不同产区的枳壳药材中类柠檬苦素类成分的含量差异较大,成分含量与产地呈相关性。结论湖南、江西的枳壳类柠檬苦素类含量较高。
Objective To determine the content of limonin and nomilin in Fructus Aurantii from different places, and to provide evidence for the quality control evaluation of Fructus Aurantii. Methods HPLC was adopted. Limonin and nomilin were separated on a C18 column(250 mm×4.6 mm, 5 μm) with the mobile phase of acetonitrile-water-tetrahydrofuran-glacial acetic acid(41∶59∶1∶0.2) at 1.0 m L·min-1, and the detection wavelength was 225 nm. Limomin and nomilin were taken as indexes for the cluster analysis. Results Contents of medicinal components in Fructus Aurantii from different localities were significantly different. Correlation between content of each component and locality was found. Conclusion Qualities and contents of Fructus Aurantii from Jiangxi and Hunan are higher than those from other places.
Objective To determine the content of limonin and nomilin in Fructus Aurantii from different places, and to provide evidence for the quality control evaluation of Fructus Aurantii. Methods HPLC was adopted. Limonin and nomilin were separated on a C18 column(250 mm×4.6 mm, 5 μm) with the mobile phase of acetonitrile-water-tetrahydrofuran-glacial acetic acid(41∶59∶1∶0.2) at 1.0 m L·min-1, and the detection wavelength was 225 nm. Limomin and nomilin were taken as indexes for the cluster analysis. Results Contents of medicinal components in Fructus Aurantii from different localities were significantly different. Correlation between content of each component and locality was found. Conclusion Qualities and contents of Fructus Aurantii from Jiangxi and Hunan are higher than those from other places.
引文
[1]中国药典2015年版.一部[S].2015:246.
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[4]郭增喜,李文庭,李兆奎.不同产地枳壳中柚皮苷和新橙皮苷的测定[J].中草药,2012,43(7):1347-1348.
[5]杨武亮,杨世林,黄加龙,等.高效液相色谱法测定不同产地枳壳中柚皮苷和新橙皮苷[J].中草药,2006,37(12):1888-1889.
[6]施学骄,刘友平.不同产地枳实、枳壳中柠檬苦素含量比较研究[J].中国食品添加剂,2011,28(5):125-128.
[7]李云,邢丽娜,周明眉,等.柑橘不同用药部位中橙皮苷、柠檬苦素及诺米林含量与其体外抗氧化和抗乳腺癌活性相关性研究[J].上海中医药杂志,2015,49(6):87-90.
[2]王一帆.是谁推高了枳壳价格[J].全国药材信息,2014,(25):6.
[3]施学骄.酸橙果实不同采收期化学成分动态变化及枳实,枳壳药材质量评价研究[D].成都:成都中医药大学,2012.
[4]郭增喜,李文庭,李兆奎.不同产地枳壳中柚皮苷和新橙皮苷的测定[J].中草药,2012,43(7):1347-1348.
[5]杨武亮,杨世林,黄加龙,等.高效液相色谱法测定不同产地枳壳中柚皮苷和新橙皮苷[J].中草药,2006,37(12):1888-1889.
[6]施学骄,刘友平.不同产地枳实、枳壳中柠檬苦素含量比较研究[J].中国食品添加剂,2011,28(5):125-128.
[7]李云,邢丽娜,周明眉,等.柑橘不同用药部位中橙皮苷、柠檬苦素及诺米林含量与其体外抗氧化和抗乳腺癌活性相关性研究[J].上海中医药杂志,2015,49(6):87-90.