土壤水分含量和接种摩西斗管囊霉(Funneliformis mosseae)对伯乐树幼苗生长的影响
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摘要
该研究测定了伯乐树在不同水分处理(40%、60%、80%、100%)和时间节点下的菌根侵染率,不同水分处理前后接种组和对照组幼苗株高、地径、叶片数等形态指标以及叶片丙二醛含量、幼苗成活率指标。结果表明:(1)相对含水量60%条件下,菌丝、丛枝和总的侵染率最高,40%低水分含量和80%以上高水分含量都不利于菌根真菌对幼苗的侵染。(2)在40%和60%低水分量含量条件下,接种幼苗的丙二醛含量显著低于对照,接种摩西斗管囊霉可提高幼苗对干旱胁迫的抗耐性,但80%和100%高水分含量条件下接种对幼苗的作用不明显。(3)幼苗在自然和接种条件下适宜生长的土壤相对含水量为80%和60%。(4)相同水分条件下,接种幼苗成活率高于对照,其中相对含水量60%下接种幼苗的成活率最高(90%),在40%低水分含量或100%饱和水分含量下,幼苗的成活率都相对较低。
In this paper,the mycorrhizal infection rates of Bretschneidera sinensis under different water treatments( 40%,60%,80%,100%) and time points were determined. Quantified morphological characteristics of the seedlings such as height,ground diameter,and number of leaves,together with malonaldehyde content and survival rate in the inoculation group and the control group before and after different water treatments were also determined. The results were as follows:( 1) Under the condition of relative soil moisture content of 60%,the infection rates of mycelium,arbuscula and the total were the highest,low moisture content( 40%) and high moisture content( ≥80%) were not conducive to mycorrhizal fungi on the seedlings infestation.( 2) The content of malondialdehyde in the inoculated seedlings was significantly lower than that of the control at 40% and 60% treatments,which suggested that inoculation with Funneliformis mosseae could increase seedling resistance to drought stress,but with no obvious effect on treatments of 80% and 100%high moisture content.( 3) The relative water content of soil suitable for seedling growth under natural and inoculated conditions was 80% and 60%,respectively.( 4) Under the same water conditions,the survival rate of inoculated seedlings was higher than that of the control,with the highest( 90%) under 60% treatment,while the survival rate of seedlings was relatively low at 40% low moisture content and 100% saturated moisture content.
In this paper,the mycorrhizal infection rates of Bretschneidera sinensis under different water treatments( 40%,60%,80%,100%) and time points were determined. Quantified morphological characteristics of the seedlings such as height,ground diameter,and number of leaves,together with malonaldehyde content and survival rate in the inoculation group and the control group before and after different water treatments were also determined. The results were as follows:( 1) Under the condition of relative soil moisture content of 60%,the infection rates of mycelium,arbuscula and the total were the highest,low moisture content( 40%) and high moisture content( ≥80%) were not conducive to mycorrhizal fungi on the seedlings infestation.( 2) The content of malondialdehyde in the inoculated seedlings was significantly lower than that of the control at 40% and 60% treatments,which suggested that inoculation with Funneliformis mosseae could increase seedling resistance to drought stress,but with no obvious effect on treatments of 80% and 100%high moisture content.( 3) The relative water content of soil suitable for seedling growth under natural and inoculated conditions was 80% and 60%,respectively.( 4) Under the same water conditions,the survival rate of inoculated seedlings was higher than that of the control,with the highest( 90%) under 60% treatment,while the survival rate of seedlings was relatively low at 40% low moisture content and 100% saturated moisture content.
引文
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GARCA I,MENDOZA R,POMAR MC,2008.Deficit and excess of soil water impact on plant growth of Lotus tenuis by affecting nutrient uptake and arbuscular mycorrhizal symbiosis[J].Plant Soil,304:117-131.
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HE XL,ZHANG HS,ZHAO LL,2008.Effects of AM fungi and water stress on drought resistance of Artemisia ordosica in different soils[J].J Plant Ecol,32(5):994-1001.[贺学礼,张焕仕,赵丽莉,2008.不同土壤中水分胁迫和AM真菌对油蒿抗旱性的影响[J].植物生态学报,32(5):994-1001.]
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HUANG JX,ZHUANG XY,2000.Mycorrhizal study on three national secondary protection plants of Chebaling nursery[J].J S Chin Agric Univ,2:38-41.[黄久香,庄雪影,2000.车八岭苗圃三种国家二级保护植物的菌根研究[J].华南农业大学学报,2:38-41.]
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LI HS,2000.Principles and techniques of plant physiology and biochemistry experiment[M].Beijing:Higher Education Press:192-194.[李合生,2000.植物生理生化实验原理和技术[M].北京:高等教育出版社:192-194.]
LI XL,CAO YP,1993.The mechanism of VA mycorrhizal absorption of mineral nutrients[J].Soil,25(5):274-277.[李晓林,曹一平,1993.VA菌根吸收矿质养分的机制[J].土壤,25(5):274-277.]
MA JQ,WEI ZL,QIN JL,et al.,2018.Investigation on types of AM fungi in rhizospheresoil of wild Artemisia annua L.in Guangxi[J].J Southern Agric,49(5):870-876.[马俊卿,韦竹立,覃京龙,等,2018.广西野生黄花蒿根际土壤AM真菌类型调查分析[J].南方农业学报,49(5):870-876.]
MO YL,2016.The mechanism of watermalon in response to drought stress and the mitigation effict of AMF[D].Yangling:Northwest A&F University:117-119.[莫言玲,2016.西瓜对干旱胁迫的响应机制及丛枝菌根真菌的缓解效应[D].杨凌:西北农林科技大学:117-119.]
QIAO Q,GUO XF,XING FW,et al.,2015.Symbiosis effect of Glomus eburneun on seed of Bretschneidera sinensis[J].JZhejiang For Sci Technol,35(3):43-46.[乔琦,郭幸飞,邢福武,等,2015.摩西球囊霉与伯乐树的共生效应研究[J].浙江林业科技,35(3):43-46.]
QIAO Q,QIN XS,XING FW,et al.,2011.Death causes and conservation strategies of the annual regenerated seedlings of rare plant,Bretschneidera sinensis[J].Acta Ecol Sin,31(16):4709-4716.[乔琦,秦新生,邢福武,等,2011.珍稀植物伯乐树一年生更新幼苗的死亡原因和保育策略[J].生态学报,31(16):4709-4716.]
QIAO Q,WEN XY,CHEN HF,et al.,2010.Ecological anatomy of the root in Bretschneidera sinensis,a threatened endemic plant in China[J].J Wuhan Bot Res,28(5):544-549.[乔琦,文香英,陈红锋,等,2010.中国特有濒危植物伯乐树根的结构特征[J].武汉植物学研究,28(5):544-549.]
SHENOY VV,KALAGUDI GM,2005.Enhancing plant phosphorus use efficiency for sustainable cropping[J].Biotechnol Adv,23:501-513.
SMITH FW,2001.S and P transport systems in plants[J].Plant Soil,232:109-118.
SMITH SE,READ DJ,2008.Mycorrhizal symbiosis[M].London:Academic Press.
WANG B,QIU YL,2006.Phylogenetic distribution and evolution of mycorrhizas in land plants[J].Mycorrhiza,16:299-363.
WANG MQ,WU PH,SHEN YK,et al.,2018.Effects of arbuscular mycorrhizal fungus on the growth and nitrogen and phosphours acquisition of salt stressed Stevia rebaudiana[J].Chin J Appl Ecol,http://kns.cnki.net/kcms/detail/51.1482.Q.20180319.1558.015.html.[王敏强,吴沛鸿,沈益康,等,2018.盐胁迫下接种AM真菌对甜菊生长和氮磷吸收的影响[J].应用生态学报,http://kns.cnki.net/kcms/detail/51.1482.Q.20180319.1558.015.html.]
WANG S,XIE Y,2004.China species red list(Vol.I)[M].Beijing:Higher Education Press.[汪松,解焱,2004.中国物种红色名录(第一卷)[M].北京:中国高等教育出版社.]
WANG YS,ZHANG SB,ZHANG MQ,2012.China arbuscular mycorrhizal fungi and resources[M].Beijing:China Agriculture Publishing House:175-176.[王幼珊,张淑彬,张美庆,2012.中国丛枝菌根真菌资源与种质资源[M].北京:中国农业出版社:175-176.]
WU MK,YANG HY,LONG W,et al.,2006.A study on the seedling cultivation of Bretschneidera sinensis Hemsl.[J].Guizhou For Sci Technol,34(4):39-41+38.[伍铭凯,杨汉远,龙舞,等,2006.伯乐树种子育苗试验[J].贵州林业科技,34(4):39-41+38.]
WU QS,2006.Study on effect and mechanism of arbuscular mycorrihizal fungi in drought resistance of citrus[D].Wuhan:Huazhong Agricultural University:36-40.[吴强盛,2006.丛枝菌根真菌对柑橘抗旱性的作用及其机理研究[D].武汉:华中农业大学:36-40.]
WU ZY,1991.The areal-types of Chinese genera of seed plants[J].Acta Bot Yunnan,13(S4):1-3.
XU PH,WANG FQ,QI YG,et al.,2017.The effects of mycorrhizal fungi on the drought resistance of Camellia sinensis L.[J].Acta Agric Boreal-Occident Sin,26(7):1033-1040.[许平辉,王飞权,齐玉岗,等,2017.丛枝菌根真菌对茶树抗旱性的影响[J].西北农业学报,26(7):1033-1040.]
YU YF,1999.List of national key protection wild plants(1st batch)[J].Life World,151(5):4-11.[于永福,1999.国家重点保护野生植物名录(第一批)[J].植物杂志,151(5):4-11.]
ZHANG F,NI QD,ZOU YN,et al.,2017.Preliminary study on the mechanism of AMF in enhancing the drought tolerance of plants[J].J Fungal Res,15(1):8-13.[张菲,倪秋丹,邹英宁,等,2017.AMF增强枳抗旱性作用机制的初步研究[J].菌物学报,15(1):8-13.]
ZHANG J,TIAN HL,WANG YQ,et al.,2011.Comparison of seeds and seedling growth for 3 different geographical provenances of Bretschneidera sinensis[J].Chin J Trop Agric,31(5):12-15.[张季,田华林,王玉奇,等,2011.3个不同地理种源的伯乐树种子和苗期生长差异比较[J].热带农业科学,31(5):12-15.]
ZHAO YP,YANG P,ZHU Y,et al.,2011.Effects of arbuscular mycorrhizal fungi on physiological characteristics of seedlings of different marigold(Tagetes erecta)varieties[J].Acta Agric Jiangxi,30(3):102-105.[赵永平,杨萍,朱亚,等,2018.丛枝菌根真菌对不同品种万寿菊幼苗生理特性的影响[J].江西农业学报,30(3):102-105.]
BRACHMANN A,PARNISKE M,2006.The most widespread symbiosis on earth[J].Plos Biol,4(7):1111-1112.
CHEN J,XIE J,TANG M,2014.Effects of arbuscular mycorrhizal fungi on the growth and drought resistance of Amorpha fruticosa under water stress[J].J Beijing For Univ,36(6):142-148.[陈婕,谢靖,唐明,2014.水分胁迫下丛枝菌根真菌对紫穗槐生长和抗旱性的影响[J].北京林业大学学报,36(6):142-148.]
CHEN WQ,1984.Bretschneideraceae[M].Beijing:Science Press:8-10.
FEI SL,FANG JY,FAN YJ,et al.,1999.Anatomical characteristics of leaves and woods of Fagus lucida and their relationship to ecological factors[J].Acta Bot Sin,41(9):1002-1009.[费松林,方精云,樊拥军,等,1999.贵州梵净山亮叶水青冈叶片和木材的解剖学特征及其与生态因子的关系[J].植物学报,41(9):1002-1009.]
GARCA I,MENDOZA R,POMAR MC,2008.Deficit and excess of soil water impact on plant growth of Lotus tenuis by affecting nutrient uptake and arbuscular mycorrhizal symbiosis[J].Plant Soil,304:117-131.
HARRISON MJ,1999.Molecular and cellular aspects of the arbuscular mycorrhizal symbiosis[J].Ann Rev Plant Biol,50:361-389.
HE XL,ZHANG HS,ZHAO LL,2008.Effects of AM fungi and water stress on drought resistance of Artemisia ordosica in different soils[J].J Plant Ecol,32(5):994-1001.[贺学礼,张焕仕,赵丽莉,2008.不同土壤中水分胁迫和AM真菌对油蒿抗旱性的影响[J].植物生态学报,32(5):994-1001.]
HUANG HG,LLX,ZHANG YM,et al.,2017.Microbeassisted drought resistance for tobacco plants:Mechanisms and applications[J].Chin J Appl Ecol,28(9):3099-3110.[黄化刚,吕立新,张艳茗,等,2017.微生物帮助烟草抗旱的机理及其应用[J].应用生态学报,28(9):3099-3110.]
HUANG JX,ZHUANG XY,2000.Mycorrhizal study on three national secondary protection plants of Chebaling nursery[J].J S Chin Agric Univ,2:38-41.[黄久香,庄雪影,2000.车八岭苗圃三种国家二级保护植物的菌根研究[J].华南农业大学学报,2:38-41.]
JIA ZY,YU J,DE Y,et al.,2017.The effect of arbuscular mycorrhiza fungi on drought resistance of Leymus chinensis[J].J Arid Land Resour Environ,31(1):132-136.[贾振宇,于洁,德英,等,2017.丛枝菌根真菌接种对羊草抗旱性的影响[J].干旱区资源与环境,31(1):132-136.]
LI HS,2000.Principles and techniques of plant physiology and biochemistry experiment[M].Beijing:Higher Education Press:192-194.[李合生,2000.植物生理生化实验原理和技术[M].北京:高等教育出版社:192-194.]
LI XL,CAO YP,1993.The mechanism of VA mycorrhizal absorption of mineral nutrients[J].Soil,25(5):274-277.[李晓林,曹一平,1993.VA菌根吸收矿质养分的机制[J].土壤,25(5):274-277.]
MA JQ,WEI ZL,QIN JL,et al.,2018.Investigation on types of AM fungi in rhizospheresoil of wild Artemisia annua L.in Guangxi[J].J Southern Agric,49(5):870-876.[马俊卿,韦竹立,覃京龙,等,2018.广西野生黄花蒿根际土壤AM真菌类型调查分析[J].南方农业学报,49(5):870-876.]
MO YL,2016.The mechanism of watermalon in response to drought stress and the mitigation effict of AMF[D].Yangling:Northwest A&F University:117-119.[莫言玲,2016.西瓜对干旱胁迫的响应机制及丛枝菌根真菌的缓解效应[D].杨凌:西北农林科技大学:117-119.]
QIAO Q,GUO XF,XING FW,et al.,2015.Symbiosis effect of Glomus eburneun on seed of Bretschneidera sinensis[J].JZhejiang For Sci Technol,35(3):43-46.[乔琦,郭幸飞,邢福武,等,2015.摩西球囊霉与伯乐树的共生效应研究[J].浙江林业科技,35(3):43-46.]
QIAO Q,QIN XS,XING FW,et al.,2011.Death causes and conservation strategies of the annual regenerated seedlings of rare plant,Bretschneidera sinensis[J].Acta Ecol Sin,31(16):4709-4716.[乔琦,秦新生,邢福武,等,2011.珍稀植物伯乐树一年生更新幼苗的死亡原因和保育策略[J].生态学报,31(16):4709-4716.]
QIAO Q,WEN XY,CHEN HF,et al.,2010.Ecological anatomy of the root in Bretschneidera sinensis,a threatened endemic plant in China[J].J Wuhan Bot Res,28(5):544-549.[乔琦,文香英,陈红锋,等,2010.中国特有濒危植物伯乐树根的结构特征[J].武汉植物学研究,28(5):544-549.]
SHENOY VV,KALAGUDI GM,2005.Enhancing plant phosphorus use efficiency for sustainable cropping[J].Biotechnol Adv,23:501-513.
SMITH FW,2001.S and P transport systems in plants[J].Plant Soil,232:109-118.
SMITH SE,READ DJ,2008.Mycorrhizal symbiosis[M].London:Academic Press.
WANG B,QIU YL,2006.Phylogenetic distribution and evolution of mycorrhizas in land plants[J].Mycorrhiza,16:299-363.
WANG MQ,WU PH,SHEN YK,et al.,2018.Effects of arbuscular mycorrhizal fungus on the growth and nitrogen and phosphours acquisition of salt stressed Stevia rebaudiana[J].Chin J Appl Ecol,http://kns.cnki.net/kcms/detail/51.1482.Q.20180319.1558.015.html.[王敏强,吴沛鸿,沈益康,等,2018.盐胁迫下接种AM真菌对甜菊生长和氮磷吸收的影响[J].应用生态学报,http://kns.cnki.net/kcms/detail/51.1482.Q.20180319.1558.015.html.]
WANG S,XIE Y,2004.China species red list(Vol.I)[M].Beijing:Higher Education Press.[汪松,解焱,2004.中国物种红色名录(第一卷)[M].北京:中国高等教育出版社.]
WANG YS,ZHANG SB,ZHANG MQ,2012.China arbuscular mycorrhizal fungi and resources[M].Beijing:China Agriculture Publishing House:175-176.[王幼珊,张淑彬,张美庆,2012.中国丛枝菌根真菌资源与种质资源[M].北京:中国农业出版社:175-176.]
WU MK,YANG HY,LONG W,et al.,2006.A study on the seedling cultivation of Bretschneidera sinensis Hemsl.[J].Guizhou For Sci Technol,34(4):39-41+38.[伍铭凯,杨汉远,龙舞,等,2006.伯乐树种子育苗试验[J].贵州林业科技,34(4):39-41+38.]
WU QS,2006.Study on effect and mechanism of arbuscular mycorrihizal fungi in drought resistance of citrus[D].Wuhan:Huazhong Agricultural University:36-40.[吴强盛,2006.丛枝菌根真菌对柑橘抗旱性的作用及其机理研究[D].武汉:华中农业大学:36-40.]
WU ZY,1991.The areal-types of Chinese genera of seed plants[J].Acta Bot Yunnan,13(S4):1-3.
XU PH,WANG FQ,QI YG,et al.,2017.The effects of mycorrhizal fungi on the drought resistance of Camellia sinensis L.[J].Acta Agric Boreal-Occident Sin,26(7):1033-1040.[许平辉,王飞权,齐玉岗,等,2017.丛枝菌根真菌对茶树抗旱性的影响[J].西北农业学报,26(7):1033-1040.]
YU YF,1999.List of national key protection wild plants(1st batch)[J].Life World,151(5):4-11.[于永福,1999.国家重点保护野生植物名录(第一批)[J].植物杂志,151(5):4-11.]
ZHANG F,NI QD,ZOU YN,et al.,2017.Preliminary study on the mechanism of AMF in enhancing the drought tolerance of plants[J].J Fungal Res,15(1):8-13.[张菲,倪秋丹,邹英宁,等,2017.AMF增强枳抗旱性作用机制的初步研究[J].菌物学报,15(1):8-13.]
ZHANG J,TIAN HL,WANG YQ,et al.,2011.Comparison of seeds and seedling growth for 3 different geographical provenances of Bretschneidera sinensis[J].Chin J Trop Agric,31(5):12-15.[张季,田华林,王玉奇,等,2011.3个不同地理种源的伯乐树种子和苗期生长差异比较[J].热带农业科学,31(5):12-15.]
ZHAO YP,YANG P,ZHU Y,et al.,2011.Effects of arbuscular mycorrhizal fungi on physiological characteristics of seedlings of different marigold(Tagetes erecta)varieties[J].Acta Agric Jiangxi,30(3):102-105.[赵永平,杨萍,朱亚,等,2018.丛枝菌根真菌对不同品种万寿菊幼苗生理特性的影响[J].江西农业学报,30(3):102-105.]