海洋细菌L_1-9菌株在日本囊对虾养殖池中的定殖及其抑菌和水质净化作用
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摘要
将海洋多粘类芽孢杆菌L1-9菌株接种在日本囊对虾养殖池水体中,以不接L1-9菌株为对照组,定期检测水体中L1-9菌株、弧菌、芽孢杆菌及其他异养细菌数量以及氨氮、硝酸盐、亚硝酸盐和磷酸盐的质量浓度和日本囊对虾的成活率。试验结果表明,在日本囊对虾养殖池水体中,随着培养时间的延长,接种L1-9菌株池水中,芽孢杆菌、异养细菌和弧菌的数量以及氨氮、硝酸盐、亚硝酸盐含量显著低于对照组,磷酸盐的质量浓度显著高于对照组,其中对芽孢杆菌和氨氮的影响最为明显,培养第5d处理组芽孢杆菌的菌落数为4.60×102 cfu/mL显著低于对照组的1.80×103 cfu/mL;培养第7d处理组的氨氮质量浓度为60.2μg/L,显著低于对照组的76.7μg/L,表明L1-9菌株在水产养殖中具有一定抑菌及水质净化作用,L1-9菌株对日本囊对虾成活率没有影响。
Marine Paenibacillus polymyxa strain L1-9 was inoculated(without inoculation as control)in Japanese shrimp culture ponds to investigate the colonization,bacteriostasis and purification water of the marine P.polymyxastrain L1-9in the Japanese shrimp culture ponds.After that,the number of L1-9,Vibrio,Bacillaceae and heterotrophic bacteria was detected regularly,and the concentrations of ammonia nitrogen,nitrate,nitrite,and phosphate were detected.During the culture,the number of Vibrio,Bacillaceae,and heterotrophic bacteria and the levels of ammonia nitrogen,nitrate,and nitrite were found to be significantly lower in the Japanese shrimp culture ponds inoculated with L1-9 strain than those in the control.However,there was significantly higher concentration of phosphate in the Japanese shrimp culture ponds inoculated with L1-9 strain than that in the control pond,especially the number of Bacillaceae and the ammonia nitrogen level,the Bacillaceae reaching 4.60×102 cfu/mL on the 5th d,significantly less than that in the control(1.80×103 cfu/mL)on the 7th d.The concentration of ammonia nitrogen was found to be 60.2μg/L,less than that in the control(76.7μg/L).It is concluded that L1-9strain has obviously bacteriostasis and purification water effect in aquaculture.
Marine Paenibacillus polymyxa strain L1-9 was inoculated(without inoculation as control)in Japanese shrimp culture ponds to investigate the colonization,bacteriostasis and purification water of the marine P.polymyxastrain L1-9in the Japanese shrimp culture ponds.After that,the number of L1-9,Vibrio,Bacillaceae and heterotrophic bacteria was detected regularly,and the concentrations of ammonia nitrogen,nitrate,nitrite,and phosphate were detected.During the culture,the number of Vibrio,Bacillaceae,and heterotrophic bacteria and the levels of ammonia nitrogen,nitrate,and nitrite were found to be significantly lower in the Japanese shrimp culture ponds inoculated with L1-9 strain than those in the control.However,there was significantly higher concentration of phosphate in the Japanese shrimp culture ponds inoculated with L1-9 strain than that in the control pond,especially the number of Bacillaceae and the ammonia nitrogen level,the Bacillaceae reaching 4.60×102 cfu/mL on the 5th d,significantly less than that in the control(1.80×103 cfu/mL)on the 7th d.The concentration of ammonia nitrogen was found to be 60.2μg/L,less than that in the control(76.7μg/L).It is concluded that L1-9strain has obviously bacteriostasis and purification water effect in aquaculture.
引文
[1]Maloy K,Sahu N S,Swarnakumar K.Probiotics in aquaculture:importance and future perspectives[J].Indian Microbiol,2008(48):299-308.
[2]张云,宁丽军,谢晓晖,等.芽孢杆菌在沼虾养殖水体中益水作用的研究[J].饲料工业,2010,31(10):27-30.
[3]张博.不同种类芽孢杆菌净化水质试验[J].饲料工业,2011,32(10):55-57.
[4]黄建华,周发林,马之明,等.微生物制剂对斑节对虾亲虾池异养细菌的影响[J].生态学杂志,2007,26(6):826-830.
[5]夏振强,暴增海,周超,等.抗真菌海洋细菌L1-9菌株的几种胞外酶活性测定[J].河南农业科学,2009(7):74-77.
[6]夏振强,暴增海,王增池,等.海洋细菌L1-9菌株产蛋白酶发酵条件的优化[J].湖北农业科学,2009,48(5):1129-1131.
[7]马桂珍,王淑芳,暴增海,等.海洋多黏类芽孢杆菌L1-9菌株抗菌蛋白的分离纯化及其抗菌作用[J].食品科学,2010,31(17):335-339.
[8]马桂珍,王淑芳,暴增海,等.多粘类芽孢杆菌L1-9菌株对番茄早疫病的抑菌防病作用[J].中国蔬菜,2010(12):55-59.
[9]马桂珍,暴增海,王淑芳.海洋细菌L1-9菌株对小麦的促生防病作用研究[J].中国生物防治学报,2011,27(1):228-232.
[10]马桂珍,付泓润,王淑芳,等.海洋多粘类芽孢杆菌L1-9菌株抗菌物质产生条件及其抑菌谱研究[J].食品科学,2012,33(19):231-235.
[11]浦寅芳,孔德平,王增池,等.抗植物病原真菌海洋细菌L1-9菌株的发酵条件优化[J].安徽农业科学,2008,36(15):6385-6386.
[12]章洁香,张瑜斌,张才学,等.高位虾池水体细菌和弧菌的数量变化及影响因素[J].集美大学学报,2010,15(5):327-333.
[13]袁翠霖,李卓佳,杨莺莺,等.芽孢杆菌制剂对养殖前期罗非鱼池塘微生物群落代谢功能的影响[J].生态学杂志,2010,29(12):2464-2470.
[14]李秋芬,曲克明,陈碧鹃,等.老化虾池生态系中几类主要细菌的季节变化特征[J].海洋水产研究,2002,23(2):12-18.
[15]詹晓燕,刘臣辉,范海燕,等.水体中氨氮测定方法的比较[J].环境科学与管理,2010,35(1):132-135.
[16]尹伦甫.养殖水体氨氮超标的危害及菌藻联合调控技术[J].科学养鱼,2012(1):89.
[17]陈红菊,岳永生,丁雷,等.生物净化剂对养殖水体亚硝酸盐含量影响的研究[J].淡水渔业,2003,33(1):16-18.
[18]明晶.芽孢杆菌在改良水体环境中的作用[J].科学养鱼,2006(4):76.
[2]张云,宁丽军,谢晓晖,等.芽孢杆菌在沼虾养殖水体中益水作用的研究[J].饲料工业,2010,31(10):27-30.
[3]张博.不同种类芽孢杆菌净化水质试验[J].饲料工业,2011,32(10):55-57.
[4]黄建华,周发林,马之明,等.微生物制剂对斑节对虾亲虾池异养细菌的影响[J].生态学杂志,2007,26(6):826-830.
[5]夏振强,暴增海,周超,等.抗真菌海洋细菌L1-9菌株的几种胞外酶活性测定[J].河南农业科学,2009(7):74-77.
[6]夏振强,暴增海,王增池,等.海洋细菌L1-9菌株产蛋白酶发酵条件的优化[J].湖北农业科学,2009,48(5):1129-1131.
[7]马桂珍,王淑芳,暴增海,等.海洋多黏类芽孢杆菌L1-9菌株抗菌蛋白的分离纯化及其抗菌作用[J].食品科学,2010,31(17):335-339.
[8]马桂珍,王淑芳,暴增海,等.多粘类芽孢杆菌L1-9菌株对番茄早疫病的抑菌防病作用[J].中国蔬菜,2010(12):55-59.
[9]马桂珍,暴增海,王淑芳.海洋细菌L1-9菌株对小麦的促生防病作用研究[J].中国生物防治学报,2011,27(1):228-232.
[10]马桂珍,付泓润,王淑芳,等.海洋多粘类芽孢杆菌L1-9菌株抗菌物质产生条件及其抑菌谱研究[J].食品科学,2012,33(19):231-235.
[11]浦寅芳,孔德平,王增池,等.抗植物病原真菌海洋细菌L1-9菌株的发酵条件优化[J].安徽农业科学,2008,36(15):6385-6386.
[12]章洁香,张瑜斌,张才学,等.高位虾池水体细菌和弧菌的数量变化及影响因素[J].集美大学学报,2010,15(5):327-333.
[13]袁翠霖,李卓佳,杨莺莺,等.芽孢杆菌制剂对养殖前期罗非鱼池塘微生物群落代谢功能的影响[J].生态学杂志,2010,29(12):2464-2470.
[14]李秋芬,曲克明,陈碧鹃,等.老化虾池生态系中几类主要细菌的季节变化特征[J].海洋水产研究,2002,23(2):12-18.
[15]詹晓燕,刘臣辉,范海燕,等.水体中氨氮测定方法的比较[J].环境科学与管理,2010,35(1):132-135.
[16]尹伦甫.养殖水体氨氮超标的危害及菌藻联合调控技术[J].科学养鱼,2012(1):89.
[17]陈红菊,岳永生,丁雷,等.生物净化剂对养殖水体亚硝酸盐含量影响的研究[J].淡水渔业,2003,33(1):16-18.
[18]明晶.芽孢杆菌在改良水体环境中的作用[J].科学养鱼,2006(4):76.