硫酸奎宁荧光探针在Fe~(3+)检测中的应用
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摘要
硫酸奎宁作为荧光试剂,最大激发波长为350 nm,最大发射峰位于450 nm处。硫酸奎宁与不同浓度的Fe~(3+)混合后,发生荧光猝灭,lg(I0/I)与c(Fe~(3+))呈良好的线性关系,基于此建立了硫酸奎宁荧光探针检测Fe~(3+)的新方法。研究表明,该方法检测Fe~(3+)浓度的线性范围为0. 7～400μmol/L,相关系数为R=0. 999 5,检测限为140 nmol/L。在实际样品分析中,当分别加入4、10和70μmol/L Fe~(3+)时,回收率为99. 4%～100. 4%,相对标准偏差(RSD)为0. 8%～2. 1%。该方法操作简便、反应速度快、灵敏度高,能用于实际样品中Fe~(3+)的检测分析。
As a fluorescent reagent,quinine sulfate has a maximum excitation wavelength of 350 nm and a maximum emission peak of 450 nm.When quinine sulfate was mixed with different concentrations of Fe~(3+),the fluorescence of quinine sulfate was quenched.It was found that lg( I0/I) showed a good linear relationship with c( Fe~(3+)).Based on this,a new method was developed for the detection of Fe~(3+)by quinine sulfate as a fluorescent probe.The linear range of Fe~(3+)concentration was 0. 7 ～ 400 μmol/L with a correlation coefficient of 0. 999 5.The limit of detection was140 nmol/L.When 4,10,70 μmol/L Fe~(3+)were added to the real samples,the recovery range was 99. 4% ～ 100. 4% and the relative standard deviation( RSD) range was 0. 8% ～ 2. 1%. This method was simple,rapid and sensitive and can be used for the detection of Fe~(3+)in real samples.
As a fluorescent reagent,quinine sulfate has a maximum excitation wavelength of 350 nm and a maximum emission peak of 450 nm.When quinine sulfate was mixed with different concentrations of Fe~(3+),the fluorescence of quinine sulfate was quenched.It was found that lg( I0/I) showed a good linear relationship with c( Fe~(3+)).Based on this,a new method was developed for the detection of Fe~(3+)by quinine sulfate as a fluorescent probe.The linear range of Fe~(3+)concentration was 0. 7 ～ 400 μmol/L with a correlation coefficient of 0. 999 5.The limit of detection was140 nmol/L.When 4,10,70 μmol/L Fe~(3+)were added to the real samples,the recovery range was 99. 4% ～ 100. 4% and the relative standard deviation( RSD) range was 0. 8% ～ 2. 1%. This method was simple,rapid and sensitive and can be used for the detection of Fe~(3+)in real samples.
引文
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[2]张文宇,常青,周雨锋,等.一步合成硫、氮共掺杂的碳量子点及其在Fe3+检测中的应用[J].发光学报,2016,37(4):410-415.
[3]HARDIKAR P S,JOSHI S M,BHAT D S,et al.Spuriously high prevalence of prediabetes diagnosed by Hb A1c in young indians partly explained by hematological factors and iron deficiency anemia[J]. Diabetes Care,2012,35(4):797-802.
[4]KALINOWSK D S,RICHARDSON D R.Future of toxicology-iron chelators and differing modes of action and toxicity:the changing face of iron chelation therapy[J].Chem.Res.Toxicol.,2007,20(5):715-720.
[5]赵冰,刘婷,王丽艳,等. Schiff碱链接的双菲并咪唑Fe3+荧光探针的合成及识别性能研究[J].化学试剂,2017,39(1):1-5.
[6]FILIPPATOS G,FARMAKIS D,COLET J C,et al.Intravenous ferric carboxymaltose in iron-deficient chronic heart failure patients with and without anaemia:a subanalysis of the FAIR-HF trial[J].Eur.J.Heart Fail.,2013,15(11):1 267-1 276.
[7]TESFALDET Z O,STADEN J F,STEFAN R I.Sequential injection spectrophotometric determination of iron as Fe(Ⅱ)in multi-vitamin preparations using 1,10-phenanthroline as complexing agent[J]. Talanta,2004,64(5):1 189-1 195.
[8]EVANS E H,DAWSON J B,FISHER A,et al.Advances in atomic emission,absorption and fluorescence spectrometry and related techniques[J]. J. Anal. Atom. Spectrom.,2002,17(6):622-651.
[9]XU H,HU H C,CAO C S,et al. Lanthanide organic framework as a regenerable luminescent probe for Fe3+[J].Inorg.Chem.,2015,54(10):4 585-4 587.
[10]MICHALSKI R. Applications of ion chromatography for the determination of inorganic cations[J].Crit.Rev.Anal.Chem.,2009,39(4):230-250.
[11]BAI R C,GAO W,BAI S D,et al. A novel fluorescent Fe3+sensor based on a europium complex[J]. Opt. Mater.,2013,35(5):833-836.
[12]LIN V S,LIPPERT A R,CHANG C J. Cell-trappable fluorescent probes for endogenous hydrogen sulfide signaling and imaging H2O2-dependent H2S production[J].P.Natl.Acad.Sci.USA,2013,110(18):7 131-7 135.
[13]尹苹苹,艾可龙,李美丽,等.基于高荧光碳纳米粒子的Fe3+超灵敏检测[J].分析化学,2014,42(10):1 427-1 433.
[14]KAGIT R,YILDIRIM M,OZAY O,et al. Phosphazene based multicentered naked-eye fluorescent sensor with high selectivity for Fe3+ion[J]. Inorg. Chem.,2014,53(4):2 144-2 151.
[15]HOU B L,TIAN D,LIU J F,et al.A water-stable metalorganic framework for highly sensitive and selective sensing of Fe3+ion[J]. Inorg. Chem.,2016,55(20):10 580-10 586.
[16]沈文通,成春文,王风贺,等.一种新型罗丹明类双通道荧光探针及其对Fe3+和Cu2+的选择性识别研究[J].化学试剂,2017,39(8):861-866.
[17]YAO Q F,LV B Z,JI C D,et al.Supramolecular hostguest system as ratiometric Fe3+ion sensor based on water-soluble pillar[5]arene[J]. Acs. Appl. Mater. Interfaces,2017,9(41):36 320-36 326.