海藻多糖的单糖组成对体外抗氧化活性的影响
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摘要
比较广西北部湾石莼(Ulva lactuca L.)、海带(Laminaria japonica)、裙带菜(Undaria pinnatifida Surin-gar)、紫菜(Porphyra)的单糖组成及抗氧化活性的差异,揭示多糖结构与其体外抗氧化活性的关系。利用PMP柱前衍生化HPLC分析海藻多糖的单糖组成,采用羟自由基清除试验、超氧阴离子自由基清除试验及DPPH自由基清除试验指征其体外抗氧化活性,结果表明,4种海藻多糖的单糖组成在主成分空间分布离散,石莼及紫菜主要由葡萄糖组成,海带主要由甘露糖组成,裙带菜则主要由半乳糖组成;其体外抗氧化活性存在显著差异,裙带菜多糖对DPPH的清除能力(半抑制浓度IC_(50)值为0. 56±0. 02 mg/mL)显著高于其他3种海藻多糖;石莼、裙带菜与海带对羟自由基均有较强的清除活性,而紫菜多糖对羟自由基的清除能力较差(IC_(50)值为26. 59±0. 98mg/mL);石莼与裙带菜对超氧阴离子的清除活性较强,显著高于海带与紫菜,其中石莼显著高于裙带菜,IC_(50)值分别为1. 61±0. 17、2. 73±0. 06 mg/mL。相关性分析及冗余分析结果表明,对抗氧化活性影响较为显著的因子为葡萄糖(Glc)、核糖(Rib)、木糖(Xyl)(P <0. 01)。
To discuss the difference in monosaccharide composition and antioxidant activity among different algal polysaccharides,we analyzed the Ulva lactuca L.,Laminaria japonica,Undaria pinnatifida Suringar and Porphyra of Beibu Gulf in Guangxi. The monosaccharide compositions were analyzed by HPLC applied to PMP precolumn derivation. The antioxidant activity of polysaccharides was indicated by the scavenging DPPH,hydroxyl( ·OH~-),and superoxide anions( O_2~(-·)) activity from algae polysaccharide. Results indicated that four algal polysaccharides were significantly different in monosaccharide composition by PCA analysis. Ulva lactuca L. and Porphyra were mainly composed by Glucosec,Laminaria japonica and Undaria pinnatifida Suringar was composed by Mannoseand Galactose,respectively. The scavenging capacity of DPPH of Undaria pinnatifida Suringar( the hemi-inhibitory concentration IC_(50) was 0. 56 ± 0. 02 mg/mL) was significantly higher than that of Ulva lactuca L.,Laminaria japonica and Porphyra. The scavenging ability for( ·OH-)of Porphyra polysaccharide( IC_(50) was 26. 59 ± 0. 98 mg/mL) was significantly lower than that of Ulva lactuca L.,Laminaria japonica,Undaria pinnatifida and Suringar. According to the scavenging superoxide anions( O_2~(-·)) activity,Ulva lactuca L. was significantly higher than Undaria pinnatifida Suringar,and the value of IC_(50) of Ulva lactuca L. and Undaria pinnatifida Suringar were 1. 61 ± 0. 17 mg/mL and 2. 73 ± 0. 06 mg/mL,respectively. Besides,Pearson correlation analysis and redundancy analysis both showed that glucose( Glc),ribose( Rib),xylose( Xyl) significantly influenced the antioxidant activity of algae polysaccharide( P < 0. 01).
To discuss the difference in monosaccharide composition and antioxidant activity among different algal polysaccharides,we analyzed the Ulva lactuca L.,Laminaria japonica,Undaria pinnatifida Suringar and Porphyra of Beibu Gulf in Guangxi. The monosaccharide compositions were analyzed by HPLC applied to PMP precolumn derivation. The antioxidant activity of polysaccharides was indicated by the scavenging DPPH,hydroxyl( ·OH~-),and superoxide anions( O_2~(-·)) activity from algae polysaccharide. Results indicated that four algal polysaccharides were significantly different in monosaccharide composition by PCA analysis. Ulva lactuca L. and Porphyra were mainly composed by Glucosec,Laminaria japonica and Undaria pinnatifida Suringar was composed by Mannoseand Galactose,respectively. The scavenging capacity of DPPH of Undaria pinnatifida Suringar( the hemi-inhibitory concentration IC_(50) was 0. 56 ± 0. 02 mg/mL) was significantly higher than that of Ulva lactuca L.,Laminaria japonica and Porphyra. The scavenging ability for( ·OH-)of Porphyra polysaccharide( IC_(50) was 26. 59 ± 0. 98 mg/mL) was significantly lower than that of Ulva lactuca L.,Laminaria japonica,Undaria pinnatifida and Suringar. According to the scavenging superoxide anions( O_2~(-·)) activity,Ulva lactuca L. was significantly higher than Undaria pinnatifida Suringar,and the value of IC_(50) of Ulva lactuca L. and Undaria pinnatifida Suringar were 1. 61 ± 0. 17 mg/mL and 2. 73 ± 0. 06 mg/mL,respectively. Besides,Pearson correlation analysis and redundancy analysis both showed that glucose( Glc),ribose( Rib),xylose( Xyl) significantly influenced the antioxidant activity of algae polysaccharide( P < 0. 01).
引文
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3 Usov A,Smirnova GP,Klochkova NG.Algae polysaccharides 55 polysaccharide composition of some brown Kamchatka algae[J].Bioorganicheskaia Khimiia,2001,27:444.
4 Li HM,Du Y,Min CJ,et al.Antioxidant and antimicrobial properties of polysaccharide from houttuynia cordata leaf[J].Nat Prod Res Dev(天然产物研究与开发),2017,29:1745-1751.
5 Zhao H,Xiao Z,Chen ZH,et al.Research progress on the hypolipidemic effect of marine bioactive substances[J].Farm Products Processing(农产品加工),2017,1:92-96.
6 Liu N,Sun Y,Zeng S,et al.Research progress on bioactive substances and its biological activity of algae[J].Food Safe Qual Detec Technol(食品安全质量检测学报),2015,6:2875-2879.
7 Jiang HY,Wu SC,Cen YZ.Nutrient component analysis of six kinds of economic algae in Zhanjiang sea area[J].Guangzhou Chem Ind(广州化工),2011,7:105-108.
8 Ma J,Hou P,Chen Yan,et al.Study on antioxidant activity and anti-lipid peroxidation effect of several algal polysaccharides[J].South Chin Fish Sci(南方水产科学),2017,13:97-104.
9 Wang JL,Guo HY,Zhang J,et al.Sulfated modification,characterization and structure-antioxidant relationships of Artemisia sphaerocephala polysaccha-rides[J].Carbohyd Polym,2010,81:897-905.
10 Ni LJ,Wang YY,He WY,et al.Monosaccharide composition,activity and their correlation analysis in eight polysaccharides[J].J Tianjin Univ:Sci and Tech(天津大学学报:自科与技术版),2014,47:326-330.
11 Feng XZ,Wu SG,Kong J,et al.Optimization of phenol-sulfuric acid determination conditions of polysaccharide in Ulva lactuca L.by orthogonal test[J].Strait Pharm J(海峡药学),2013,25(12):94-96.
12 Han W,Jiang RZ,Chen YH,et al.Comparison of three chromatographic methods for monosaccharide composition analysis of Tremella polysaccharide[J].Nat Prod Res Dev(天然产物研究与开发),2012,24:359-361.
13 Feng XZ,Chen Y,Wu SG.Analysis of monosaccharide compositions in Ulva lactuca L.polysaccharides[J].Sci Tech of Food Ind(食品工业科技),2014,35(7):91-94.
14 Feng XZ,Wu SG,Kong J.Study on ultrasonic assisted extraction of Ulva lactuca L.and its scavenging activity of DPPHfree radical[J].J Chin Med Mater(中药材),2013,36:1870-1872.
15 Zhang JH,Yao Z,Sun Y,et al.Optimization of polysaccharide extraction from Mulberry fungus and evaluation of its probiotic and antioxidant activities[J].Mod Food Sci and Tech(现代食品科技),2018,34:143-149.
16 Chen M,Zhang HD,Chen M.Studies on the anti-oxidative activities of different solvents extracts from Chinese Herbal Medicine[J].Acta Sci Nat Univ Sunyaseni(中山大学学报:自科版),2006,45:131-133.
17 Han SH,Zhu JB,Wang YY.Measurement of the antioxidant activity by pyrogallol autoxidation[J].China Brewing(中国酿造),2009,28:155-157.
18 Yao LY,Zhao QS,Xiao J,et al.Composition and antioxidant activity of the polysaccharides from cultivated Saussurea involucrate[J].Int J Biol Macromol,2012,50:849-853.