不同浓度PEG对甜菜种子萌发的影响
|
摘要
为研究不同浓度PEG-6000在23℃时对甜菜种子萌发的影响,为甜菜种子引发研究提供参考,本研究以甜菜种子TD305为试验材料,分别采用浓度为10%、20%、40%、60%的PEG-6000,时间分别为24、48、72 h对甜菜种子进行处理,测定其发芽势、发芽率、发芽指数、活力指数。结果显示,处理时间为24 h时,40%和60%的PEG-6000对甜菜种子萌发有促进作用;处理时间为48 h时,20%和40%的PEG-6000促进甜菜种子萌发;处理时间为72 h时,10%、20%和40%的PEG-6000可促进甜菜种子萌发。PEG处理比对表明,40%PEG-6000浸泡72 h后的发芽势、发芽率和活力指数最高,60%PEG-6000浸泡24 h后的发芽指数最高。常温时,适宜浓度PEG-6000可提高甜菜种子的活力指标。
To study the effect of different concentrations of PEG-6000 on the germination of sugar beet seed at23℃, and lay the foundation for sugar beet seed initiation research, in this study, the sugar beet seed of TD305 was used as the experimental material, the concentrations of PEG-6000 were 10%, 20%, 40% and 60% and the treatment time was 24 h, 48 h and 72 h, respectively. The germination potential, germination rate,germination index and vigor index were determined. The results showed that 40% and 60% of PEG-6000 promoted the germination of sugar beet seed by 24 h. 20% and 40% of PEG-6000 promoted the germination of beet seed by 48 h. 10%, 20% and 40% of PEG-6000 promoted the germination of beet seed by 72 h. The PEG treatment showed that the germination potential, germination rate and vigor index were the highest after soaking in 40% of PEG-6000 for 72 h. The germination index was the highest after soaking in 60% of PEG-6000 for 24 h. At room temperature, the optimum concentration of PEG-6000 could improve the vigor index of sugar beet seed.
To study the effect of different concentrations of PEG-6000 on the germination of sugar beet seed at23℃, and lay the foundation for sugar beet seed initiation research, in this study, the sugar beet seed of TD305 was used as the experimental material, the concentrations of PEG-6000 were 10%, 20%, 40% and 60% and the treatment time was 24 h, 48 h and 72 h, respectively. The germination potential, germination rate,germination index and vigor index were determined. The results showed that 40% and 60% of PEG-6000 promoted the germination of sugar beet seed by 24 h. 20% and 40% of PEG-6000 promoted the germination of beet seed by 48 h. 10%, 20% and 40% of PEG-6000 promoted the germination of beet seed by 72 h. The PEG treatment showed that the germination potential, germination rate and vigor index were the highest after soaking in 40% of PEG-6000 for 72 h. The germination index was the highest after soaking in 60% of PEG-6000 for 24 h. At room temperature, the optimum concentration of PEG-6000 could improve the vigor index of sugar beet seed.
引文
[1] Rajjou L, Duval M, Gallardo K, et al. Seed germination and vigor[J]. Annu Rev Plant Biol,2012,63:507-533.
[2]张培岭,白羽嘉,黄伟,等.不同浓度外源水杨酸对甜瓜果实抗病相关酶活性的影响[J].食品科技,2016,41(7):38-43.
[3]陈贵华,张少英,朱芳慧,等.不同种子活化剂对甜菜种子萌发特性的影响[J].作物杂志,2017(3):171-174.
[4] Liu H X, Guo Z G, Wang Y R. Optimal conditions for hydro priming Lucerne seeds[J]. New Zealand Journal of Agricultutal Rsearch,2008,51:69-75.
[5]宋子叶,郭尚敬,杨万立,等.不同引发剂对小麦种子发芽的影响[J].河北农业科学,2018,22(4):13-15,21.
[6]邸娜,张耀强,李旭红,等.种子引发对盐胁迫下向日葵种子萌发和幼苗生理特性的影响[J].河南农业科学,2018,47(4):43-48.
[7]祁瑞林,张红瑞,彭涛,等.不同引发剂对贮藏夏枯草种子引发效果的影响[J].浙江农业科学,2018,59(2):283-286.
[8]李盈.聚乙二醇(PEG)处理对植物种子的影响[J].甘肃农业,2014,(14):65-66.
[9]郑安俭,王州飞,张红生.作物种子萌发生理与遗传研究进展[J].江苏农业学报,2017,33(1):218-223.
[10]董志朋,刘顼,唐玉姣,等.我国种子引发技术在作物抗旱性上的研究进展[J].种子,2016,35(10):53-58.
[11]沙红,高燕,高卫时,等.聚乙二醇对甜菜种子低温萌发的影响[J].中国糖料,2013(3):40-41,43.
[12]米兴旺,陈超,张彦龙.种子引发及其效应的研究现状[J].农业与技术,2014,34(5):31-32.
[13] Heydecker W. Germination of an idea:the priming of seeds[R].University of Nottingham School of Agriculture Report,1974:50-67.
[14]董志朋,刘顼,唐玉姣,等.我国种子引发技术在作物抗旱性上的研究进展[J].种子,2016,35(10):53-58.
[15]张峻琳,王琳,汪天宇,等.聚乙二醇引发对生菜高温下种子萌发的影响[J].北京农学院学报,2017,32(2):34-38.
[16]周缘娣,黄良伟,吴小松,等.PEG引发对镉胁迫下玉米种子萌发及幼苗生长的影响[J].现代农业科技,2017(13):13-14.
[17]杨雪桐,张永强,曹微,等.PEG引发种子对大豆产量及主要农艺性状的影响[J].黑龙江八一农垦大学学报,2016,28(4):6-8,35.
[18]李田,刘海河,车寒梅,等.聚乙二醇对茄子种子活力及幼苗生理特性的影响[J].河北农业大学学报,2016,39(3):14-18.
[19]蔡春菊,范少辉,曹帮华,等.PEG和GA3引发处理对老化毛竹种子理化特性的影响[J].南京林业大学学报:自然科学版,2018,42(2):40-46.
[20]夏方山,董秋丽,毛培胜,等.PEG引发对老化燕麦种胚细胞与线粒体结构及抗氧化性能的影响[J].草业学报,2018,27(5):170-177.
[21]季鹏,于靖,张军林,等.聚乙二醇(PEG)引发种子的机制及最适引发条件的选择[J].中国农村小康科技,2006(6):18-20.
[22] Obroucheva N V, Antipova O V. Physiology of the initiation of seed germination[J]. Russian J Plant Physiol,1997,44:250-264.
[23]徐恒恒,黎妮,刘树君,等.种子萌发及其调控的研究进展[J].作物学报,2014,40(7):1141-1156.
[24] Nonogaki H, Bassel G W, Bewley J D. Germination-still a mystery.Plant Sci,2010,179:574-581.
[25]陈宝悦,陈子敬,王倩.聚乙二醇6000引发对芹菜种子萌发及幼苗生长的影响[J].北方园艺,2016(6):10-13.
[26]姜生秀,严子柱,吴昊.PEG6000模拟干旱胁迫对2种沙冬青种子萌发的影响[J].西北林学院学报,2018,33(5):130-136.
[2]张培岭,白羽嘉,黄伟,等.不同浓度外源水杨酸对甜瓜果实抗病相关酶活性的影响[J].食品科技,2016,41(7):38-43.
[3]陈贵华,张少英,朱芳慧,等.不同种子活化剂对甜菜种子萌发特性的影响[J].作物杂志,2017(3):171-174.
[4] Liu H X, Guo Z G, Wang Y R. Optimal conditions for hydro priming Lucerne seeds[J]. New Zealand Journal of Agricultutal Rsearch,2008,51:69-75.
[5]宋子叶,郭尚敬,杨万立,等.不同引发剂对小麦种子发芽的影响[J].河北农业科学,2018,22(4):13-15,21.
[6]邸娜,张耀强,李旭红,等.种子引发对盐胁迫下向日葵种子萌发和幼苗生理特性的影响[J].河南农业科学,2018,47(4):43-48.
[7]祁瑞林,张红瑞,彭涛,等.不同引发剂对贮藏夏枯草种子引发效果的影响[J].浙江农业科学,2018,59(2):283-286.
[8]李盈.聚乙二醇(PEG)处理对植物种子的影响[J].甘肃农业,2014,(14):65-66.
[9]郑安俭,王州飞,张红生.作物种子萌发生理与遗传研究进展[J].江苏农业学报,2017,33(1):218-223.
[10]董志朋,刘顼,唐玉姣,等.我国种子引发技术在作物抗旱性上的研究进展[J].种子,2016,35(10):53-58.
[11]沙红,高燕,高卫时,等.聚乙二醇对甜菜种子低温萌发的影响[J].中国糖料,2013(3):40-41,43.
[12]米兴旺,陈超,张彦龙.种子引发及其效应的研究现状[J].农业与技术,2014,34(5):31-32.
[13] Heydecker W. Germination of an idea:the priming of seeds[R].University of Nottingham School of Agriculture Report,1974:50-67.
[14]董志朋,刘顼,唐玉姣,等.我国种子引发技术在作物抗旱性上的研究进展[J].种子,2016,35(10):53-58.
[15]张峻琳,王琳,汪天宇,等.聚乙二醇引发对生菜高温下种子萌发的影响[J].北京农学院学报,2017,32(2):34-38.
[16]周缘娣,黄良伟,吴小松,等.PEG引发对镉胁迫下玉米种子萌发及幼苗生长的影响[J].现代农业科技,2017(13):13-14.
[17]杨雪桐,张永强,曹微,等.PEG引发种子对大豆产量及主要农艺性状的影响[J].黑龙江八一农垦大学学报,2016,28(4):6-8,35.
[18]李田,刘海河,车寒梅,等.聚乙二醇对茄子种子活力及幼苗生理特性的影响[J].河北农业大学学报,2016,39(3):14-18.
[19]蔡春菊,范少辉,曹帮华,等.PEG和GA3引发处理对老化毛竹种子理化特性的影响[J].南京林业大学学报:自然科学版,2018,42(2):40-46.
[20]夏方山,董秋丽,毛培胜,等.PEG引发对老化燕麦种胚细胞与线粒体结构及抗氧化性能的影响[J].草业学报,2018,27(5):170-177.
[21]季鹏,于靖,张军林,等.聚乙二醇(PEG)引发种子的机制及最适引发条件的选择[J].中国农村小康科技,2006(6):18-20.
[22] Obroucheva N V, Antipova O V. Physiology of the initiation of seed germination[J]. Russian J Plant Physiol,1997,44:250-264.
[23]徐恒恒,黎妮,刘树君,等.种子萌发及其调控的研究进展[J].作物学报,2014,40(7):1141-1156.
[24] Nonogaki H, Bassel G W, Bewley J D. Germination-still a mystery.Plant Sci,2010,179:574-581.
[25]陈宝悦,陈子敬,王倩.聚乙二醇6000引发对芹菜种子萌发及幼苗生长的影响[J].北方园艺,2016(6):10-13.
[26]姜生秀,严子柱,吴昊.PEG6000模拟干旱胁迫对2种沙冬青种子萌发的影响[J].西北林学院学报,2018,33(5):130-136.