刮痧对皮肤组织形态和GCS、SP、SOD表达的影响
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摘要
目的:探讨刮痧对人体皮肤组织形态及皮肤中相关化学成分表达变化的影响。方法:募集健康受试者12名,男女各半。用刮痧板沿左侧膀胱经自心俞穴至肾俞穴由上向下刮拭出痧,刮痧后在背部刮痧区和对侧非刮痧区分别采取3mm×3mm×3mm的圆柱型皮肤,分别进行肥大细胞(MC)、降钙素基因相关肽(CGRP)和鬼笔环肽(PHA)等荧光免疫组织化学染色,并以ELISA法测定皮肤中糖皮质激素(GCS)、P物质(SP)及超氧化物歧化酶(SOD)的含量。结果:刮痧后皮肤外观、表皮层厚度和血管组织形态均有明显差异,主要表现为皮肤肿胀、表皮层增厚、血管变形等;MC标记到的阳性细胞减少,并伴随脱颗粒现象;CGRP阳性神经纤维表达无显著差异。同时,与非刮痧区相比,刮痧区皮肤组织中GCS、SP、SOD含量显著上升(P<0.05)。结论:刮痧疗法对皮肤安全,刮痧局部皮肤组中MC颗粒及GCS、SOD等多种化学物质表达可能形成效应联系,发挥治疗作用。
Objective: To investigate the effect of guasha therapy on changes in the skin tissue morphology and expression of related chemical components of the human skin. Methods: A total of 12 healthy subjects, half men and half women, were scraped with guasha plate along the left side of bladder meridian from acupoint Xinshu(BL15) to Shenshu(BL23) point until the sha coming out. After scraping, the cylindrical skin tissue about 3 mm length × 3 mm width × 3 mm high on the back of sha district and non sha district were taken. Subsequently, mast cell(MC), calcitonin gene related peptide(CGRP) and palloidin(PHA) were demonstrated by using immunofluorescence staining. And glucocorticoids(GCS), substance P(SP), superoxide dismutase(SOD) were detected by using enzyme-linked immunosorbent assay(ELISA). Results: After scraping, the appearance of skin, the thickness of the epidermis and the form of vascular tissue were obviously different. The main manifestations were skin swelling, the thickening of the epidermis, and the deformation of the blood vessels. The number of positive cells marked by MC was reduced, accompanied by degranulation. There was no significant difference in the expression of CGRP positive nerve fibers. Simultaneously, compared with non sha district, the level of GCS, SP and SOD increased significantly on the back of sha district(P<0.05). Conclusion: Guasha therapy is safe to the skin. The expression of MC particles, GCS, SOD and other chemicals in gua sha skin district may form the effect of contact, which plays a therapeutic effect.
Objective: To investigate the effect of guasha therapy on changes in the skin tissue morphology and expression of related chemical components of the human skin. Methods: A total of 12 healthy subjects, half men and half women, were scraped with guasha plate along the left side of bladder meridian from acupoint Xinshu(BL15) to Shenshu(BL23) point until the sha coming out. After scraping, the cylindrical skin tissue about 3 mm length × 3 mm width × 3 mm high on the back of sha district and non sha district were taken. Subsequently, mast cell(MC), calcitonin gene related peptide(CGRP) and palloidin(PHA) were demonstrated by using immunofluorescence staining. And glucocorticoids(GCS), substance P(SP), superoxide dismutase(SOD) were detected by using enzyme-linked immunosorbent assay(ELISA). Results: After scraping, the appearance of skin, the thickness of the epidermis and the form of vascular tissue were obviously different. The main manifestations were skin swelling, the thickening of the epidermis, and the deformation of the blood vessels. The number of positive cells marked by MC was reduced, accompanied by degranulation. There was no significant difference in the expression of CGRP positive nerve fibers. Simultaneously, compared with non sha district, the level of GCS, SP and SOD increased significantly on the back of sha district(P<0.05). Conclusion: Guasha therapy is safe to the skin. The expression of MC particles, GCS, SOD and other chemicals in gua sha skin district may form the effect of contact, which plays a therapeutic effect.
引文
[1]王莹莹,杨金生.刮痧文献整理与刮痧现代研究.北京:中国医药科技出版社,2015:47
[2]曾上劼.经络刮痧前后白细胞变化分析.黑龙江中医药,2003(1):41
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[5]徐东升.刮痧对大鼠局部皮肤组织形态学和nNOS、5-HT、HA表达影响的研究.咸阳:陕西中医药大学,2015
[6]李永明.关于肥大细胞与经穴现象的“宋氏理论”及研究方向.中国针灸,2016,36(10):1063
[7]陈波,李明月,赵雪,等.P物质与针灸效应的研究进展.上海针灸杂志,2014,33(1):82
[8]程斌,石宏,吉长福,等.与急性胃黏膜损伤相关体表敏化穴位的动态分布观察.针刺研究,2010,35(3):193-197
[9]Shinbara H,Okubo M,Kimura K,et al.Participation of calcitonin gene related peptide released via axon reflex in the local increase in muscle blood flow following manual acupuncture.Acupunct Med,2013,31(1):81-87
[10]Andrzej Slominski,Jacobo Wortsman,Ralf Paus,et al.Skin as an endocrine organ:implications for its function.Drug Discov Today Dis Mech,2008,5(2):137-144
[11]Brazzini B,Ghersetich I,Hercogova J,et al.The neuro-immunecutaneous-endocrinenetwork:relationship between mind and skin.Dermatol Ther,2003,16(2):123-131
[12]崔向清.刮痧疗法对胆红素、SOD、IL-1、IL-6、白细胞、单胺类神经递质的影响初探.中医药学报,2013,41(1):35
[13]吴智聪,刘诗雅,李良慧,等.中医刮痧渗出物中免疫成分及含量的研究.广州中医药大学学报,2017,34(2):211
[14]徐唯.糖皮质激素与免疫功能的关系及在运动免疫学中的作用.中国组织工程研究与临床康复,2008,12(5):954
[15]董亮,何永志,王远亮,等.超氧化物歧化酶(SOD)的应用研究进展.中国农业科技导报,2013,15(5):53-58
[2]曾上劼.经络刮痧前后白细胞变化分析.黑龙江中医药,2003(1):41
[3]Nielsen Arya,Knoblauch Nicola T M,Dobos Gustav J,et al.The effect of Gua Sha treatment on the microcirculation of surface tissue:a pilot study in healthy subjects.Explore:The Journal of Science&Healing,2007,3(5):456-466
[4]田宇瑛,王莹莹,杨金生,等.刮痧对家兔皮肤血流灌注量及组织形态学影响的比较研究.中医外治杂志,2009,18(6):9
[5]徐东升.刮痧对大鼠局部皮肤组织形态学和nNOS、5-HT、HA表达影响的研究.咸阳:陕西中医药大学,2015
[6]李永明.关于肥大细胞与经穴现象的“宋氏理论”及研究方向.中国针灸,2016,36(10):1063
[7]陈波,李明月,赵雪,等.P物质与针灸效应的研究进展.上海针灸杂志,2014,33(1):82
[8]程斌,石宏,吉长福,等.与急性胃黏膜损伤相关体表敏化穴位的动态分布观察.针刺研究,2010,35(3):193-197
[9]Shinbara H,Okubo M,Kimura K,et al.Participation of calcitonin gene related peptide released via axon reflex in the local increase in muscle blood flow following manual acupuncture.Acupunct Med,2013,31(1):81-87
[10]Andrzej Slominski,Jacobo Wortsman,Ralf Paus,et al.Skin as an endocrine organ:implications for its function.Drug Discov Today Dis Mech,2008,5(2):137-144
[11]Brazzini B,Ghersetich I,Hercogova J,et al.The neuro-immunecutaneous-endocrinenetwork:relationship between mind and skin.Dermatol Ther,2003,16(2):123-131
[12]崔向清.刮痧疗法对胆红素、SOD、IL-1、IL-6、白细胞、单胺类神经递质的影响初探.中医药学报,2013,41(1):35
[13]吴智聪,刘诗雅,李良慧,等.中医刮痧渗出物中免疫成分及含量的研究.广州中医药大学学报,2017,34(2):211
[14]徐唯.糖皮质激素与免疫功能的关系及在运动免疫学中的作用.中国组织工程研究与临床康复,2008,12(5):954
[15]董亮,何永志,王远亮,等.超氧化物歧化酶(SOD)的应用研究进展.中国农业科技导报,2013,15(5):53-58