星点设计-效应面法优选龙钻通痹方总生物碱提取工艺
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摘要
目的:利用星点设计-效应面法优选龙钻通痹方总生物碱的最佳提取工艺。方法:以乙醇体积分数、溶剂用量和提取时间为自变量,以提取液中总生物碱(以青藤碱计)、青藤碱含量的加权总评归一值(OD)为因变量,采用星点设计-效应面法优选龙钻通痹方总生物碱的最佳提取工艺。结果:最佳提取工艺为加入9倍量75%乙醇,回流提取2次,每次130 min。结论:采用星点设计-效应面法优选龙钻通痹方总生物碱的提取工艺方法简便,具有良好的预测性。
Objective: To optimize the extraction process for the total alkaloids of Longzuan Tongbi Fang by central composite design and response surface method. Methods: The independent variables were the ethanol contention,solvent consumption and extraction time,and the dependent variable was the weighted overall desirability of the content of total alkaloids and kukoline. The extraction process for the total alkaloids of Longzuan Tongbi Fang was optimized by central composite design and response surface method. Results: The optimum extraction conditions were as follows: 9-fold amount of solvent was used,refluxed for 130 min,extracted twice,and the ethanol concentration was 75%. Conclusion: The central composite design and response surface method used for the extraction optimization for the total alkaloids of Longzuan Tongbi Fang is very simple and highly predictive.
Objective: To optimize the extraction process for the total alkaloids of Longzuan Tongbi Fang by central composite design and response surface method. Methods: The independent variables were the ethanol contention,solvent consumption and extraction time,and the dependent variable was the weighted overall desirability of the content of total alkaloids and kukoline. The extraction process for the total alkaloids of Longzuan Tongbi Fang was optimized by central composite design and response surface method. Results: The optimum extraction conditions were as follows: 9-fold amount of solvent was used,refluxed for 130 min,extracted twice,and the ethanol concentration was 75%. Conclusion: The central composite design and response surface method used for the extraction optimization for the total alkaloids of Longzuan Tongbi Fang is very simple and highly predictive.
引文
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2 谭安蔷,刘源焕,庞宇舟,等.星点设计-效应面法优选壮药龙钻通痹颗粒的提取工艺[J].广西中医药,2014,37(1):74-76
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13 方琳乔,卢凌春,龙盛京.HPLC法同时测定两面针中氯化两面针碱与乙氧基白屈菜红碱的含量[J].西北药学杂志,2011,26(1):18-20
14 瞿发林,董文燊,刘蓉,等.不同产地青风藤中青藤碱的含量比较[J].中国药业,2010,19(18):32-33
15 吴伟,崔光华.星点设计-效应面优化法及在药学中的应用[J].国外医学:药学分册,2000,27(5):292-298
16 吴伟,崔光华,陆彬.实验设计中多指标的优化:星点设计和总评“归一值”的应用[J].中国药学杂志,2000,35(8):530-533
17 梁晋如,张新新,朱砂,等.星点设计-响应面法优化山茱萸总苷的提取及纯化工艺[J].药物分析杂志,2013,33(6):1059-1066
18 刘欣,杨新,张丽,等.星点设计-效应面法优化半枝莲总生物碱提取工艺[J].江汉大学学报(自然科学版),2016,44(5):448-455
19张金莲,谢日健,刘艳菊,等.星点设计-效应面法优选蜜糠炙黄芪的炮制工艺[J].中国实验方剂学杂志,2016,22(19):14-18
2 谭安蔷,刘源焕,庞宇舟,等.星点设计-效应面法优选壮药龙钻通痹颗粒的提取工艺[J].广西中医药,2014,37(1):74-76
3 郝小燕,彭琳,叶兰,等.飞龙掌血生物总碱抗炎镇痛作用的研究[J].中西医结合学报,2004,2(6):450-452
4 广西壮族自治区革命委员会卫生局.广西本草选编[M].南宁:广西人民出版社,1974:1382
5 张威,徐红梅,任娜,等.八角枫对佐剂性关节炎大鼠的治疗作用及毒性[J].合肥工业大学学报(自然科学版),2012,35(6):832-836
6樊洁,李海霞,王炳义,等.两面针中化学成分的分离鉴定及活性测定[J].沈阳药科大学学报,2013,30(2):100-105,131
7 刘溦溦,朱尧,汪悦.青风藤生物碱的活性成分及药理作用研究进展[J].辽宁中医杂志,2016,43(8):1765-1769
8 徐双枝,张婷,高家荣,等.多指标综合加权评分法优选补骨脂-肉豆蔻药对水提工艺研究[J].中国医院药学杂志,2016,36(18):1558-1563
9 孙嘉婧,唐灿,王子雯,等.多指标综合考察巴中产栀子提取工艺[J].中国医院药学杂志,2012,32(4):255-259
10 黄元红,卫天喜,张发生,等.星点设计-效应面法优选丹参提取工艺[J].中国实验方剂学杂志,2010,16(17):28-31
11 张振巍,白丹丹,张娜娜,等.苗药飞龙掌血中白屈菜红碱的含量测定方法[J].中国药师,2013,16(7):998-999
12 胡玉霞,和芳,余启荣,等.HPLC测定制八角枫中八角枫碱的含量[J].中国实验方剂学杂志,2011,17(12):56-58
13 方琳乔,卢凌春,龙盛京.HPLC法同时测定两面针中氯化两面针碱与乙氧基白屈菜红碱的含量[J].西北药学杂志,2011,26(1):18-20
14 瞿发林,董文燊,刘蓉,等.不同产地青风藤中青藤碱的含量比较[J].中国药业,2010,19(18):32-33
15 吴伟,崔光华.星点设计-效应面优化法及在药学中的应用[J].国外医学:药学分册,2000,27(5):292-298
16 吴伟,崔光华,陆彬.实验设计中多指标的优化:星点设计和总评“归一值”的应用[J].中国药学杂志,2000,35(8):530-533
17 梁晋如,张新新,朱砂,等.星点设计-响应面法优化山茱萸总苷的提取及纯化工艺[J].药物分析杂志,2013,33(6):1059-1066
18 刘欣,杨新,张丽,等.星点设计-效应面法优化半枝莲总生物碱提取工艺[J].江汉大学学报(自然科学版),2016,44(5):448-455
19张金莲,谢日健,刘艳菊,等.星点设计-效应面法优选蜜糠炙黄芪的炮制工艺[J].中国实验方剂学杂志,2016,22(19):14-18