不同炮制方法对白术药材指标成分含量及浸出物影响研究
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摘要
目的:研究不同炮制方法对白术药材指标成分含量及浸出物含量的影响。方法:采用HPLC色谱法测定白术药材指标成分,并采用醇溶性浸出物测定法(热浸法)测定其浸出物。结果:比较不同炮制方法对白术指标成分含量及浸出物的影响,发现米泔水漂白术的白术内酯Ⅰ、白术内酯Ⅱ、白术内酯Ⅲ含量明显增高,焦炒白术和麸炒白术中白术内酯Ⅲ含量显著增高;几种不同白术炮制品中苍术酮含量变化不明显。浸出物含量变化趋势:焦白术>白术药材>麸炒白术>土炒白术>米泔水漂白术。结论:不同炮制方法对白术药材中白术内酯Ⅰ、白术内酯Ⅱ、白术内酯Ⅲ、苍术酮及浸出物含量均有一定的影响,该研究为炮制工艺改进及临床用药提供依据。
Objective: To Study the impact of index component content and extract of Atractylodes macrocephala Koidz. with different processing methods. Method: Determinating the atractylodes medicine index ingredients with HPLC,and using the method of determination of alcohol soluble extract( hot dip method) to determine its extract. Comparing the impact of different processing methods on the index component content and the extract. Results: AtractylenlideⅠ,atractylenlideⅡ and atractylenlideⅢ of Mi Ganwei Shuipiao Atractylodes increased obviously,atractylenlideⅢ of stir-baking simply and stir-baking with branincreased obviously. There was no significant change in the content of Atractylodes ketone. Extract content: The focal fried atractylodes > Atractylodes macrocephala > Stir fried atractylodes macrocephala > Fried atractylodes macrocephala > Stir-baking with bran > Mi Ganwei Shuipiao Atractylodes. Conclusion: The different processing methods can effect on the content of the raw materials,such as the AtractylenlideⅠ,atractylenlideⅡ、atractylenlideⅢ and the extract,and provide the basis for the improvement of the processing technology and the clinical medication.
Objective: To Study the impact of index component content and extract of Atractylodes macrocephala Koidz. with different processing methods. Method: Determinating the atractylodes medicine index ingredients with HPLC,and using the method of determination of alcohol soluble extract( hot dip method) to determine its extract. Comparing the impact of different processing methods on the index component content and the extract. Results: AtractylenlideⅠ,atractylenlideⅡ and atractylenlideⅢ of Mi Ganwei Shuipiao Atractylodes increased obviously,atractylenlideⅢ of stir-baking simply and stir-baking with branincreased obviously. There was no significant change in the content of Atractylodes ketone. Extract content: The focal fried atractylodes > Atractylodes macrocephala > Stir fried atractylodes macrocephala > Fried atractylodes macrocephala > Stir-baking with bran > Mi Ganwei Shuipiao Atractylodes. Conclusion: The different processing methods can effect on the content of the raw materials,such as the AtractylenlideⅠ,atractylenlideⅡ、atractylenlideⅢ and the extract,and provide the basis for the improvement of the processing technology and the clinical medication.
引文
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[2]吴慧,赵文龙,单国顺,等.HPLC波长切换法同时测定白术及其不同麸制品中白术内酯Ⅰ、Ⅱ、Ⅲ[J].中成药,2013,35(11):2484-2487.
[3]阎克里,朱秀卿,赵丽.HPLC法同时测定白术挥发油中5种抗肿瘤成分含量[J].药物分析杂志,2011,31(6):1 106-1 110.
[4]向小庆,叶红.白术抗肿瘤作用的研究及应用进展[J].中国实验方剂学杂志,2013,19(8):367-370.
[5]李雯,尹华.白术化学成分的药理作用研究进展[J].海峡药学,2012,24(3):9-11.
[6]彭腾,李鸿翔,邓赟,等.白术内酯类成分及其药理作用研究进展[J].中国药房,2012,23(39):3 732-3 734.
[7]段启,许冬谨,谢晨.HPLC法测定白术不同炮制品中白术内酯Ⅰ、Ⅱ、Ⅲ[J].中草药,2008,39(9):2 482-2 487.
[8]沈建涛,张振凌.白术炮制品中白术内酯Ⅰ、Ⅲ含量随放置时间变化的研究[J].时珍国医国药,2014,25(8):1 873-1 875.
[9]张金莲,谢日健,刘明贵,等.不同炮制方法对白术中白术内酯Ⅰ,Ⅱ,Ⅲ含量的影响[J].中国实验方剂学杂志,2016,22(21):15-18.
[10]朱国琼,高岚.白术古今炮制述要[J].基层中药杂志,1998,12(3):17.
[11]李钊,李维聪.浅析白术不同炮制的临床应用[J].四川中医,2015,33(2):189-191.
[12]费莹,王娜妮,李洪玉,等.炮制工艺对白术挥发油组成及3种白术内酯含量的影响研究[J].中华中医药学刊,2016,34(2):418-421.
[13]王文明,夏黎,段启,等.清炒白术高效液相色谱指纹图谱研究[J].现代医药卫生,2013,29(17):2 582-2 583.
[14]段启,许冬谨,刘传祥,等.白术的研究进展[J].中草药,2008,39(5):附4-附6.
[15]伏新顺.白术炮制不同功有别[N].中国中医药报,2009-09-17(5).
[16]李庆.白术的不同炮制法对临床作用影响[J].中国医药指南,2013,11(19):302-303.
[17]张钰祺,龚千锋.米泔水在中药炮制中的古今应用研究[J].江西中医药,2011,42(4):64-66.
[18]谌瑞林,何浪,费俗青,等.白术不同炮制品白术内酯含量的比较研究[J].时珍国医国药,2016,27(12):2 911-2 914.